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The map label for this gene is mrdA [H]

Identifier: 82701451

GI number: 82701451

Start: 341633

End: 343573

Strand: Reverse

Name: mrdA [H]

Synonym: Nmul_A0317

Alternate gene names: 82701451

Gene position: 343573-341633 (Counterclockwise)

Preceding gene: 82701452

Following gene: 82701450

Centisome position: 10.79

GC content: 56.21

Gene sequence:

>1941_bases
ATGAATCACAGAATCGAGCTTCGAAGCTACTCGCGTGAGTTACACAATTTCCGCCTGCGGCTGGCGATAAGCGCCGGCTT
CATGCTGCTGCTCTTCCTGCTGCTGTTCGCACGCTTCTTTTACCTGCAGGTTTCTCAGCGTGAGCATTATCACACGCTGG
CGGAAGCAAATCGCATTTCCATTGCGCCGATCGTTCCCAACCGCGGCCTGATTTTTGACCGCAATGGCGTCGTGCTCGCT
CATAATTATTCTGCCTATACACTCGAAATCGTTCCGAGCAGGATCGGAAATCTCGAGCGCCTCATCAATGAGCTGACGAC
GGTAGTGGAGATCTCCGCGCGTGACCGCAAGCGCTTCAAGAAATTGATGGAGGAGAACAGAAGATTCGAAAGCTTGCCTA
TTCGCACGCGATTATCGGACGTGGAGGTTGCCCGCTTCGCCGCCAACCGCTATCGCTTTCCGGGAGTGGAAATCAAGGCG
CGGCCGTTCCGCCAGTATCCCAAAGGCGAGTTCGCCTCTCACGTGGTGGGTTACATAGGGCGCATCAATGACAAGGATCT
GGAACGGCTGGAGGCTGACAGCGAACTCGCCAATTACCGCGGTTCCCAGCACATGGGAAAAATCGGCATCGAACAGAGCT
ATGAGAGGGAATTGCACGGCATCACCGGAATCGAGGAGATGGAAACCGACGCAGGCGGCCGTGCAATTCGCGTCATATCC
CGCACACCGCCGATTGCGGGCAGGAATCTCAAGCTATCGCTCGATTTCAAATTGCAGGAAGTCGCGGAAAAGGCGTTTGG
CAACCGGCGTGGCGCACTTGTCGCCATAGAACCGGCAACGGGAGACGTGCTCGCATTCGTGAGCAGACCGGGGTTCGATC
CGAATCTTTTTGTAGACGGGATCGACACCGAGAATTGGGAATTGCTGAACAATTCCATCGATCGGCCGCTGAACAATCGG
GCTTTGCGTGGCGTATACCCGCCCGGCTCGACGTTCAAACCCTTCATGGCTCTGGCTGGTCTGGAACTCAAGAAGCGTTG
GCCGGGAAGCGCGATCAGCGATCCCGGCTACTTCAGCCTGCCCGGAAGCACTCACCGTTTTCGCGACTGGAAAGCCGGCG
GTCACGGCCTCGTGGATCTCCACAAATCGCTGGTTATCTCTTGCGACACTTATTACTATTCGCTGGCTCATGACCTCGGG
ATCGATAATATCTACAATTTTACCAGTCAGTTCGGGTTGGGGAAAAAGACCGGGATCGATATCGAAGGCGAAGCCGCCGG
CCTGTTGCCCTCTCAGGAGTGGAAAATGCAACGGCATAAACAGAACTGGTTTGCGGGCGATACGATTTCTGTGGGGATTG
GCCAGGGTTATAATCTGACCACGCCGCTGCAACTGGCATTTGCAACCGCTGTGCTTGCCGGCAACGGCGCTGCGTTCCGT
CCCCATCTCGTCAAGCAGGTCCTGGACAGTAACAACCGGATCGTACGGGAATTCGGCACAAGTCCGCTGTACCGGCTCAA
ACTCGATCCCGCCAATTTGAAGGCGGTACATAATGCGCTGGTCGATGTAACCCGTCCGGGCGGCACAGCCGCGGTCGCCG
GTGCAGGGGCCGCTTATACCTTTGCCGGAAAAACGGGGACTGCACAGGTAGTGGGGGTAAAACAGGGGGAAAAGTATGAG
GAAAGCAAGCTCGATGAACGGCATCGTGATCATGCGCTATTTATAAGCTATGCTCCCGCTGAAAACCCCAAAATTGCGCT
GTCCGTTCTGGTGGAAAATGGCGGCCATGGCGGTTCGGCAGCGGCACCGATTGCCCGCGTGGTGATGGATTACTATCTGC
TGGGTAAACTGCCTAAGGAAGCAGCTGCACAGCCTGTCGATCAGGTGGACGAAGAGGAGGATGAGGAGCACCAGCAGAAC
CGGGAGGACGGGCATGGCTAG

Upstream 100 bases:

>100_bases
GGAACTTCTTTCTTGGCAGCATTGTCGGCATGTTACTGTGGCCGCTTCTCTCAGCCATATTGAGGGTGCCGCAGAAACCC
TGGTCTGCTTCCGATGCAAG

Downstream 100 bases:

>100_bases
ACTTCCACACGCAGGGCACTACTTTACGCGCTACATCGATGGTCTTCTGCTTTGTGGAATTCTCCTGCTCATGGCAACGG
GACTTATCACACTTTTCAGC

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MNHRIELRSYSRELHNFRLRLAISAGFMLLLFLLLFARFFYLQVSQREHYHTLAEANRISIAPIVPNRGLIFDRNGVVLA
HNYSAYTLEIVPSRIGNLERLINELTTVVEISARDRKRFKKLMEENRRFESLPIRTRLSDVEVARFAANRYRFPGVEIKA
RPFRQYPKGEFASHVVGYIGRINDKDLERLEADSELANYRGSQHMGKIGIEQSYERELHGITGIEEMETDAGGRAIRVIS
RTPPIAGRNLKLSLDFKLQEVAEKAFGNRRGALVAIEPATGDVLAFVSRPGFDPNLFVDGIDTENWELLNNSIDRPLNNR
ALRGVYPPGSTFKPFMALAGLELKKRWPGSAISDPGYFSLPGSTHRFRDWKAGGHGLVDLHKSLVISCDTYYYSLAHDLG
IDNIYNFTSQFGLGKKTGIDIEGEAAGLLPSQEWKMQRHKQNWFAGDTISVGIGQGYNLTTPLQLAFATAVLAGNGAAFR
PHLVKQVLDSNNRIVREFGTSPLYRLKLDPANLKAVHNALVDVTRPGGTAAVAGAGAAYTFAGKTGTAQVVGVKQGEKYE
ESKLDERHRDHALFISYAPAENPKIALSVLVENGGHGGSAAAPIARVVMDYYLLGKLPKEAAAQPVDQVDEEEDEEHQQN
REDGHG

Sequences:

>Translated_646_residues
MNHRIELRSYSRELHNFRLRLAISAGFMLLLFLLLFARFFYLQVSQREHYHTLAEANRISIAPIVPNRGLIFDRNGVVLA
HNYSAYTLEIVPSRIGNLERLINELTTVVEISARDRKRFKKLMEENRRFESLPIRTRLSDVEVARFAANRYRFPGVEIKA
RPFRQYPKGEFASHVVGYIGRINDKDLERLEADSELANYRGSQHMGKIGIEQSYERELHGITGIEEMETDAGGRAIRVIS
RTPPIAGRNLKLSLDFKLQEVAEKAFGNRRGALVAIEPATGDVLAFVSRPGFDPNLFVDGIDTENWELLNNSIDRPLNNR
ALRGVYPPGSTFKPFMALAGLELKKRWPGSAISDPGYFSLPGSTHRFRDWKAGGHGLVDLHKSLVISCDTYYYSLAHDLG
IDNIYNFTSQFGLGKKTGIDIEGEAAGLLPSQEWKMQRHKQNWFAGDTISVGIGQGYNLTTPLQLAFATAVLAGNGAAFR
PHLVKQVLDSNNRIVREFGTSPLYRLKLDPANLKAVHNALVDVTRPGGTAAVAGAGAAYTFAGKTGTAQVVGVKQGEKYE
ESKLDERHRDHALFISYAPAENPKIALSVLVENGGHGGSAAAPIARVVMDYYLLGKLPKEAAAQPVDQVDEEEDEEHQQN
REDGHG
>Mature_646_residues
MNHRIELRSYSRELHNFRLRLAISAGFMLLLFLLLFARFFYLQVSQREHYHTLAEANRISIAPIVPNRGLIFDRNGVVLA
HNYSAYTLEIVPSRIGNLERLINELTTVVEISARDRKRFKKLMEENRRFESLPIRTRLSDVEVARFAANRYRFPGVEIKA
RPFRQYPKGEFASHVVGYIGRINDKDLERLEADSELANYRGSQHMGKIGIEQSYERELHGITGIEEMETDAGGRAIRVIS
RTPPIAGRNLKLSLDFKLQEVAEKAFGNRRGALVAIEPATGDVLAFVSRPGFDPNLFVDGIDTENWELLNNSIDRPLNNR
ALRGVYPPGSTFKPFMALAGLELKKRWPGSAISDPGYFSLPGSTHRFRDWKAGGHGLVDLHKSLVISCDTYYYSLAHDLG
IDNIYNFTSQFGLGKKTGIDIEGEAAGLLPSQEWKMQRHKQNWFAGDTISVGIGQGYNLTTPLQLAFATAVLAGNGAAFR
PHLVKQVLDSNNRIVREFGTSPLYRLKLDPANLKAVHNALVDVTRPGGTAAVAGAGAAYTFAGKTGTAQVVGVKQGEKYE
ESKLDERHRDHALFISYAPAENPKIALSVLVENGGHGGSAAAPIARVVMDYYLLGKLPKEAAAQPVDQVDEEEDEEHQQN
REDGHG

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=636, Percent_Identity=41.1949685534591, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI1786272, Length=657, Percent_Identity=24.6575342465753, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 71902; Mature: 71902

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHRIELRSYSRELHNFRLRLAISAGFMLLLFLLLFARFFYLQVSQREHYHTLAEANRIS
CCCEEEHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEE
IAPIVPNRGLIFDRNGVVLAHNYSAYTLEIVPSRIGNLERLINELTTVVEISARDRKRFK
EEEECCCCCEEEECCCEEEEECCCEEEEEECCHHHCCHHHHHHHHHHHEEECHHHHHHHH
KLMEENRRFESLPIRTRLSDVEVARFAANRYRFPGVEIKARPFRQYPKGEFASHVVGYIG
HHHHHCCCCCCCCCHHCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHH
RINDKDLERLEADSELANYRGSQHMGKIGIEQSYERELHGITGIEEMETDAGGRAIRVIS
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCEEEEEE
RTPPIAGRNLKLSLDFKLQEVAEKAFGNRRGALVAIEPATGDVLAFVSRPGFDPNLFVDG
CCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCEEEEC
IDTENWELLNNSIDRPLNNRALRGVYPPGSTFKPFMALAGLELKKRWPGSAISDPGYFSL
CCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHCCHHHHCCCCCCCCCCCEEEC
PGSTHRFRDWKAGGHGLVDLHKSLVISCDTYYYSLAHDLGIDNIYNFTSQFGLGKKTGID
CCCCCCCCCCCCCCCCHHHCCCCEEEEECHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
IEGEAAGLLPSQEWKMQRHKQNWFAGDTISVGIGQGYNLTTPLQLAFATAVLAGNGAAFR
CCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCC
PHLVKQVLDSNNRIVREFGTSPLYRLKLDPANLKAVHNALVDVTRPGGTAAVAGAGAAYT
HHHHHHHHCCCCEEEHHHCCCCEEEEEECCHHHHHHHHHHHEEECCCCCEEEECCCCEEE
FAGKTGTAQVVGVKQGEKYEESKLDERHRDHALFISYAPAENPKIALSVLVENGGHGGSA
ECCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCH
AAPIARVVMDYYLLGKLPKEAAAQPVDQVDEEEDEEHQQNREDGHG
HHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MNHRIELRSYSRELHNFRLRLAISAGFMLLLFLLLFARFFYLQVSQREHYHTLAEANRIS
CCCEEEHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEE
IAPIVPNRGLIFDRNGVVLAHNYSAYTLEIVPSRIGNLERLINELTTVVEISARDRKRFK
EEEECCCCCEEEECCCEEEEECCCEEEEEECCHHHCCHHHHHHHHHHHEEECHHHHHHHH
KLMEENRRFESLPIRTRLSDVEVARFAANRYRFPGVEIKARPFRQYPKGEFASHVVGYIG
HHHHHCCCCCCCCCHHCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHH
RINDKDLERLEADSELANYRGSQHMGKIGIEQSYERELHGITGIEEMETDAGGRAIRVIS
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCEEEEEE
RTPPIAGRNLKLSLDFKLQEVAEKAFGNRRGALVAIEPATGDVLAFVSRPGFDPNLFVDG
CCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCEEEEC
IDTENWELLNNSIDRPLNNRALRGVYPPGSTFKPFMALAGLELKKRWPGSAISDPGYFSL
CCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHCCHHHHCCCCCCCCCCCEEEC
PGSTHRFRDWKAGGHGLVDLHKSLVISCDTYYYSLAHDLGIDNIYNFTSQFGLGKKTGID
CCCCCCCCCCCCCCCCHHHCCCCEEEEECHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
IEGEAAGLLPSQEWKMQRHKQNWFAGDTISVGIGQGYNLTTPLQLAFATAVLAGNGAAFR
CCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCC
PHLVKQVLDSNNRIVREFGTSPLYRLKLDPANLKAVHNALVDVTRPGGTAAVAGAGAAYT
HHHHHHHHCCCCEEEHHHCCCCEEEEEECCHHHHHHHHHHHEEECCCCCEEEECCCCEEE
FAGKTGTAQVVGVKQGEKYEESKLDERHRDHALFISYAPAENPKIALSVLVENGGHGGSA
ECCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCH
AAPIARVVMDYYLLGKLPKEAAAQPVDQVDEEEDEEHQQNREDGHG
HHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]