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The map label for this gene is ampG4 [H]

Identifier: 82701357

GI number: 82701357

Start: 237385

End: 238770

Strand: Reverse

Name: ampG4 [H]

Synonym: Nmul_A0223

Alternate gene names: 82701357

Gene position: 238770-237385 (Counterclockwise)

Preceding gene: 82701358

Following gene: 82701348

Centisome position: 7.5

GC content: 59.31

Gene sequence:

>1386_bases
ATGATGCCAGCTACCCTTTCGAGCTGGCTGCACGCTTTTCGCATCTATACCCACCCCCGCGTGCTGGGAATGCTGTCTCT
CGGCTTCTCGGCGGGATTGCCCCTGCTCCTGGTGATGGGCACGCTTTCCTTCTGGCTGCGGGAAGCGGGAATCGACCGTT
CCACCATCGGCCATCTGAGCTGGATCGGGCTGGCTTACGGCTTCAAATGGATGTGGTCCCCACTCGTGGATCGCCTGCCT
TTGCCATTACTGACACAATGGCTGGGCCGGCGCCGCGCATGGCTTTTATTGTCACAAATCGTTATCGCGCTTGCCCTCAT
CGGCATCGCGCTTACCGATCCACTCCGAAACCTTTCGCATACAGTCTTTTTTGCCCTGGCGGCGGCCTTCGCCTCCGCAA
CCCAGGATATTGCCCTGGATGCATACCGGATCGAGGCGGTGGCACCCAGGCTCCAGGGGGCAATGGCCGCGACCTACCAA
GCAGGGTATCGGCTGGCAATGATTCTGGCTTCCGCCGGCGCGCTGTGGATAGCCGCCGCCGCAGGCTCATCAGCGGATAT
CTACGACTACGCGACCTGGCGTATCGCTTACCTGAGCATGGCCGCCTGCATGTCGGTGGGGATCATCACTACCCTCATCA
TCCGCGAGCCGGAGGTTCCTTTCACCCGCCTGATTTCAGAAAATGAAGATCGCGCGCGAGCCGCCATTGCGCATTGGCGG
ATCAGCGCGGCACTCAAGCAGATGCTGGCCTGGCTGTATGGAGCCGTGATCGCCCCCTTCCGAGACTTTATTGTCCGCTA
TGGGGGGCAGGCCCTGCTGATTCTCGCCCTGATCGCGGTCTACCGCATTTCCGACGTGGTAATGGGCGTCATGAGCAACC
CTTTCTACGTCGACATGGGGTATACGAAAGATGAGGTGGCGACGGTTTCCAAGGTCTATGGCGTCCTCATGACTATCGCG
GGCGCAGCGCTGGGTGGCGTTCTGACAGCAAAAATCGGCATCATGCGCACACTGTTTCTGGGAGCGATTTTGTCCGCGAC
CACCAATCTGCTGTTTGTCTGGCTGGCGGCGCGCGGGCATGACTTGACCGGCCTGATCTTCACCGTATCGGCGGACAATC
TCTCCGCCGGCATAGCATCCTCCGCTTTTATCGCTTATCTTTCCGGGCTGACCAATTCCGCCTATTCCGCTACCCAATAC
GCCCTGTTCAGCTCAGTGATGCTGCTATTGCCGAAATTTATCGCCGGATTCAGCGGAGACTTCGTGGATGCTTACGGTTA
TGCCAGCTTCTTTACCGGCACGGCGCTGCTCGGCCTGCCGGTGCTGGTTCTGGTCTGGCTGGCGGGACGTGCAAGATTCA
AGCGTGCAGAATCTCCGGAGAATTGA

Upstream 100 bases:

>100_bases
CGCTGATCATTGATTACGATTACAGCTTATCCGCCTGATCCGACGCCTTGCCGCGAACCACTGATAATGCCGGAAATCCC
GTCAGAATGACTGCCCCAGA

Downstream 100 bases:

>100_bases
AGCTTTTCCGGAAAACAGCTTTCACGAAGTACCTATTTCTGCTGTTCGAACCGGTAAAAGGCTAACATTCCCAGCAAGTT
GGGCATGAAAGTGATCTGGC

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MMPATLSSWLHAFRIYTHPRVLGMLSLGFSAGLPLLLVMGTLSFWLREAGIDRSTIGHLSWIGLAYGFKWMWSPLVDRLP
LPLLTQWLGRRRAWLLLSQIVIALALIGIALTDPLRNLSHTVFFALAAAFASATQDIALDAYRIEAVAPRLQGAMAATYQ
AGYRLAMILASAGALWIAAAAGSSADIYDYATWRIAYLSMAACMSVGIITTLIIREPEVPFTRLISENEDRARAAIAHWR
ISAALKQMLAWLYGAVIAPFRDFIVRYGGQALLILALIAVYRISDVVMGVMSNPFYVDMGYTKDEVATVSKVYGVLMTIA
GAALGGVLTAKIGIMRTLFLGAILSATTNLLFVWLAARGHDLTGLIFTVSADNLSAGIASSAFIAYLSGLTNSAYSATQY
ALFSSVMLLLPKFIAGFSGDFVDAYGYASFFTGTALLGLPVLVLVWLAGRARFKRAESPEN

Sequences:

>Translated_461_residues
MMPATLSSWLHAFRIYTHPRVLGMLSLGFSAGLPLLLVMGTLSFWLREAGIDRSTIGHLSWIGLAYGFKWMWSPLVDRLP
LPLLTQWLGRRRAWLLLSQIVIALALIGIALTDPLRNLSHTVFFALAAAFASATQDIALDAYRIEAVAPRLQGAMAATYQ
AGYRLAMILASAGALWIAAAAGSSADIYDYATWRIAYLSMAACMSVGIITTLIIREPEVPFTRLISENEDRARAAIAHWR
ISAALKQMLAWLYGAVIAPFRDFIVRYGGQALLILALIAVYRISDVVMGVMSNPFYVDMGYTKDEVATVSKVYGVLMTIA
GAALGGVLTAKIGIMRTLFLGAILSATTNLLFVWLAARGHDLTGLIFTVSADNLSAGIASSAFIAYLSGLTNSAYSATQY
ALFSSVMLLLPKFIAGFSGDFVDAYGYASFFTGTALLGLPVLVLVWLAGRARFKRAESPEN
>Mature_461_residues
MMPATLSSWLHAFRIYTHPRVLGMLSLGFSAGLPLLLVMGTLSFWLREAGIDRSTIGHLSWIGLAYGFKWMWSPLVDRLP
LPLLTQWLGRRRAWLLLSQIVIALALIGIALTDPLRNLSHTVFFALAAAFASATQDIALDAYRIEAVAPRLQGAMAATYQ
AGYRLAMILASAGALWIAAAAGSSADIYDYATWRIAYLSMAACMSVGIITTLIIREPEVPFTRLISENEDRARAAIAHWR
ISAALKQMLAWLYGAVIAPFRDFIVRYGGQALLILALIAVYRISDVVMGVMSNPFYVDMGYTKDEVATVSKVYGVLMTIA
GAALGGVLTAKIGIMRTLFLGAILSATTNLLFVWLAARGHDLTGLIFTVSADNLSAGIASSAFIAYLSGLTNSAYSATQY
ALFSSVMLLLPKFIAGFSGDFVDAYGYASFFTGTALLGLPVLVLVWLAGRARFKRAESPEN

Specific function: Probably Acts As A Permease In The Beta-Lactamase Induction System And In Peptidoglycan Recycling. [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786636, Length=451, Percent_Identity=33.2594235033259, Blast_Score=219, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49911; Mature: 49911

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPATLSSWLHAFRIYTHPRVLGMLSLGFSAGLPLLLVMGTLSFWLREAGIDRSTIGHLS
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
WIGLAYGFKWMWSPLVDRLPLPLLTQWLGRRRAWLLLSQIVIALALIGIALTDPLRNLSH
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVFFALAAAFASATQDIALDAYRIEAVAPRLQGAMAATYQAGYRLAMILASAGALWIAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEE
AGSSADIYDYATWRIAYLSMAACMSVGIITTLIIREPEVPFTRLISENEDRARAAIAHWR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHH
ISAALKQMLAWLYGAVIAPFRDFIVRYGGQALLILALIAVYRISDVVMGVMSNPFYVDMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
YTKDEVATVSKVYGVLMTIAGAALGGVLTAKIGIMRTLFLGAILSATTNLLFVWLAARGH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLTGLIFTVSADNLSAGIASSAFIAYLSGLTNSAYSATQYALFSSVMLLLPKFIAGFSGD
CCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
FVDAYGYASFFTGTALLGLPVLVLVWLAGRARFKRAESPEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMPATLSSWLHAFRIYTHPRVLGMLSLGFSAGLPLLLVMGTLSFWLREAGIDRSTIGHLS
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
WIGLAYGFKWMWSPLVDRLPLPLLTQWLGRRRAWLLLSQIVIALALIGIALTDPLRNLSH
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVFFALAAAFASATQDIALDAYRIEAVAPRLQGAMAATYQAGYRLAMILASAGALWIAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEE
AGSSADIYDYATWRIAYLSMAACMSVGIITTLIIREPEVPFTRLISENEDRARAAIAHWR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHH
ISAALKQMLAWLYGAVIAPFRDFIVRYGGQALLILALIAVYRISDVVMGVMSNPFYVDMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
YTKDEVATVSKVYGVLMTIAGAALGGVLTAKIGIMRTLFLGAILSATTNLLFVWLAARGH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLTGLIFTVSADNLSAGIASSAFIAYLSGLTNSAYSATQYALFSSVMLLLPKFIAGFSGD
CCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
FVDAYGYASFFTGTALLGLPVLVLVWLAGRARFKRAESPEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]