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The map label for this gene is exoA [H]

Identifier: 82701358

GI number: 82701358

Start: 238833

End: 239612

Strand: Reverse

Name: exoA [H]

Synonym: Nmul_A0224

Alternate gene names: 82701358

Gene position: 239612-238833 (Counterclockwise)

Preceding gene: 82701360

Following gene: 82701357

Centisome position: 7.52

GC content: 55.38

Gene sequence:

>780_bases
GTGCGAATTATAACGCTTAACCTCAACGGAATACGCTCTGCTGCCAACAAGGGTTTTTTCCGATGGCTGCCTTTGCAGGA
TGCGGATATCGTCTGCGTACAGGAACTCAAGGCTCAGGTGGGCGATATTACCGGAGAAGTGGTCTCCCCCGATGGCTACC
ACGGCTACTTCCACTGCGCTGAAAAAAGAGGATATAGCGGAGTGGGCATTTACACACGCCAGAAACCGGGGGGCGTGACC
GAGGGCCTCGGTGTACCGGAGATCGATGCGGAGGGGCGTTACCTGCGCGTCGATTTCGGGCAGTTGAGCATCATCTCGAT
CTATTTTCCGTCCGGTTCGGCGGGTGAACATCGGCAGGCCGTCAAATATTTCTTCATGGAGCAATTTTTTCCCATGCTGG
AGAAACTGGCAGCATGCGGCAGCGAGATTATTTTGTGCGGAGACTGGAACATCGCCCACAAGGAAATCGATCTCAGGAAT
TGGCGTTCCAATCAGAAGAATTCCGGCTTCCTGCCACAGGAGCGCGCCTGGCTCACCGATGTGTTTCAGGAGCTTGGATT
CGTTGATGTCTTCCGCCGGATAAATCCGGAGCCGGACCAGTATACCTGGTGGTCCAACCGCGGGCAGGCCTGGGCGAAGA
ACGTCGGCTGGCGCATCGATTATCAGATCGCCACCCCCGGGATCGCGGGCAAGGCCAACGCGGTTTCGATTTACAAGACC
GAGCGTTTCTCCGATCATTCCCCGCTGATCATTGATTACGATTACAGCTTATCCGCCTGA

Upstream 100 bases:

>100_bases
TTGGTTTGGAATTCGCCAAAGCACAGAACATTCCGGCCGATGGCGAACTCGATGAACTCCTTTCGAAAATCGGACATGAT
AATGGCTTCGGGAAATCGAC

Downstream 100 bases:

>100_bases
TCCGACGCCTTGCCGCGAACCACTGATAATGCCGGAAATCCCGTCAGAATGACTGCCCCAGAATGATGCCAGCTACCCTT
TCGAGCTGGCTGCACGCTTT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRIITLNLNGIRSAANKGFFRWLPLQDADIVCVQELKAQVGDITGEVVSPDGYHGYFHCAEKRGYSGVGIYTRQKPGGVT
EGLGVPEIDAEGRYLRVDFGQLSIISIYFPSGSAGEHRQAVKYFFMEQFFPMLEKLAACGSEIILCGDWNIAHKEIDLRN
WRSNQKNSGFLPQERAWLTDVFQELGFVDVFRRINPEPDQYTWWSNRGQAWAKNVGWRIDYQIATPGIAGKANAVSIYKT
ERFSDHSPLIIDYDYSLSA

Sequences:

>Translated_259_residues
MRIITLNLNGIRSAANKGFFRWLPLQDADIVCVQELKAQVGDITGEVVSPDGYHGYFHCAEKRGYSGVGIYTRQKPGGVT
EGLGVPEIDAEGRYLRVDFGQLSIISIYFPSGSAGEHRQAVKYFFMEQFFPMLEKLAACGSEIILCGDWNIAHKEIDLRN
WRSNQKNSGFLPQERAWLTDVFQELGFVDVFRRINPEPDQYTWWSNRGQAWAKNVGWRIDYQIATPGIAGKANAVSIYKT
ERFSDHSPLIIDYDYSLSA
>Mature_259_residues
MRIITLNLNGIRSAANKGFFRWLPLQDADIVCVQELKAQVGDITGEVVSPDGYHGYFHCAEKRGYSGVGIYTRQKPGGVT
EGLGVPEIDAEGRYLRVDFGQLSIISIYFPSGSAGEHRQAVKYFFMEQFFPMLEKLAACGSEIILCGDWNIAHKEIDLRN
WRSNQKNSGFLPQERAWLTDVFQELGFVDVFRRINPEPDQYTWWSNRGQAWAKNVGWRIDYQIATPGIAGKANAVSIYKT
ERFSDHSPLIIDYDYSLSA

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=254, Percent_Identity=35.0393700787402, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI18375503, Length=254, Percent_Identity=35.0393700787402, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI18375501, Length=254, Percent_Identity=35.0393700787402, Blast_Score=158, Evalue=4e-39,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=26.4925373134328, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71989536, Length=260, Percent_Identity=31.9230769230769, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI221330655, Length=252, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17136678, Length=252, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=4e-33,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29345; Mature: 29345

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIITLNLNGIRSAANKGFFRWLPLQDADIVCVQELKAQVGDITGEVVSPDGYHGYFHCA
CEEEEEECCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCEEEEH
EKRGYSGVGIYTRQKPGGVTEGLGVPEIDAEGRYLRVDFGQLSIISIYFPSGSAGEHRQA
HHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHH
VKYFFMEQFFPMLEKLAACGSEIILCGDWNIAHKEIDLRNWRSNQKNSGFLPQERAWLTD
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHH
VFQELGFVDVFRRINPEPDQYTWWSNRGQAWAKNVGWRIDYQIATPGIAGKANAVSIYKT
HHHHCCHHHHHHHCCCCCCCEEEECCCCCCHHHCCCEEEEEEEECCCCCCCCCEEEEEEE
ERFSDHSPLIIDYDYSLSA
CCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MRIITLNLNGIRSAANKGFFRWLPLQDADIVCVQELKAQVGDITGEVVSPDGYHGYFHCA
CEEEEEECCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCEEEEH
EKRGYSGVGIYTRQKPGGVTEGLGVPEIDAEGRYLRVDFGQLSIISIYFPSGSAGEHRQA
HHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHH
VKYFFMEQFFPMLEKLAACGSEIILCGDWNIAHKEIDLRNWRSNQKNSGFLPQERAWLTD
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHH
VFQELGFVDVFRRINPEPDQYTWWSNRGQAWAKNVGWRIDYQIATPGIAGKANAVSIYKT
HHHHCCHHHHHHHCCCCCCCEEEECCCCCCHHHCCCEEEEEEEECCCCCCCCCEEEEEEE
ERFSDHSPLIIDYDYSLSA
CCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]