BacMap

Definition	Nitrosospira multiformis ATCC 25196 chromosome, complete genome.
Accession	NC_007614
Length	3,184,243

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The map label for this gene is gmk

Identifier: 82701187

GI number: 82701187

Start: 59209

End: 59841

Strand: Reverse

Name: gmk

Synonym: Nmul_A0052

Alternate gene names: 82701187

Gene position: 59841-59209 (Counterclockwise)

Preceding gene: 82701188

Following gene: 82701186

Centisome position: 1.88

GC content: 56.24

Gene sequence:

>633_bases
ATGACTACAACGCCGGGTACTCTCTATATCATCAGCGCGCCGTCGGGAGCTGGCAAGACCAGTCTGGTCAAGGCCCTGCT
GCAAACGGGTATCGACCTCAGCCTGTCTATTTCATACACCTCACGGCAGCCTCGCCCCGAAGAAATGGACGGACGCGATT
ATCATTTCGTAACGCGCCAAGTATTCGAGCAGAAACTGCAAGAGGACGAGTTCCTGGAAAGCGCGGAACTCTACGGGAAT
TTCTACGGCACTTCGAAAAAATGGATCAATGAAACCATGACGTCGGGGCGCGACATTCTGCTGGAAATAGACAGTCAGGG
GGCGCGTCAAGTGCGGGCGGTTTTCCCCGTGGCGGTGGGCATTTTTGTTTTGCCGCCATCACTCGAAGTACTGGAAATGC
GCTTGCGCCAGCGAGCCCAGGACAGCCTCGAAGCGATCAGCCGCCGGCTGGCGGCGGCGCGTGAGGAGTTGAGTCATGCG
GGAGAGTATAACTACATCATCATCAACGACAAGCTGGACAGGGCGTTGCAGGACCTGAAATGTATCATACAGGCGGAGCG
CCTGAAAACGGCAAAGCAGCTTGTCCGCCACCATGGTCTGCTGGCGCAGCTTACCCGCACGTCCGTTTTATAA

Upstream 100 bases:

>100_bases
CTTGACCGGGCGGAAGCCTTCGGAAAGAGCGCGAGGCAATCAGAAACAAAAACCGTAACACTCACGTATTGCTGAAGAGG
GATTTCAACAGGCCTGACCA

Downstream 100 bases:

>100_bases
ACCGCACAAGCACGCGGACATACCCACCTCTGCCTCCCCCGAAAGTTTCATAGAACCATCAGCGGTAGCAACAGCCTATA
CCTTTGTCGTGTAAAATGAA

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTTTPGTLYIISAPSGAGKTSLVKALLQTGIDLSLSISYTSRQPRPEEMDGRDYHFVTRQVFEQKLQEDEFLESAELYGN
FYGTSKKWINETMTSGRDILLEIDSQGARQVRAVFPVAVGIFVLPPSLEVLEMRLRQRAQDSLEAISRRLAAAREELSHA
GEYNYIIINDKLDRALQDLKCIIQAERLKTAKQLVRHHGLLAQLTRTSVL

Sequences:

>Translated_210_residues
MTTTPGTLYIISAPSGAGKTSLVKALLQTGIDLSLSISYTSRQPRPEEMDGRDYHFVTRQVFEQKLQEDEFLESAELYGN
FYGTSKKWINETMTSGRDILLEIDSQGARQVRAVFPVAVGIFVLPPSLEVLEMRLRQRAQDSLEAISRRLAAAREELSHA
GEYNYIIINDKLDRALQDLKCIIQAERLKTAKQLVRHHGLLAQLTRTSVL
>Mature_209_residues
TTTPGTLYIISAPSGAGKTSLVKALLQTGIDLSLSISYTSRQPRPEEMDGRDYHFVTRQVFEQKLQEDEFLESAELYGNF
YGTSKKWINETMTSGRDILLEIDSQGARQVRAVFPVAVGIFVLPPSLEVLEMRLRQRAQDSLEAISRRLAAAREELSHAG
EYNYIIINDKLDRALQDLKCIIQAERLKTAKQLVRHHGLLAQLTRTSVL

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874831, Length=189, Percent_Identity=42.8571428571429, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI226874833, Length=189, Percent_Identity=42.8571428571429, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI4504221, Length=189, Percent_Identity=42.8571428571429, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI38570142, Length=189, Percent_Identity=31.7460317460317, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI21361598, Length=201, Percent_Identity=29.8507462686567, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI52630447, Length=198, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI148539628, Length=154, Percent_Identity=29.2207792207792, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI148539578, Length=154, Percent_Identity=29.2207792207792, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4557529, Length=150, Percent_Identity=29.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI192447426, Length=150, Percent_Identity=29.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI218156340, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI262118259, Length=175, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI262118255, Length=175, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI91199538, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI218156338, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI4505237, Length=175, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI262118263, Length=175, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI14780902, Length=193, Percent_Identity=23.3160621761658, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI119943106, Length=154, Percent_Identity=29.2207792207792, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI119943108, Length=151, Percent_Identity=28.476821192053, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI218156270, Length=155, Percent_Identity=30.9677419354839, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI261862237, Length=151, Percent_Identity=28.476821192053, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI111154074, Length=185, Percent_Identity=26.4864864864865, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1790080, Length=201, Percent_Identity=51.7412935323383, Blast_Score=216, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI193203132, Length=194, Percent_Identity=34.5360824742268, Blast_Score=123, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17508835, Length=194, Percent_Identity=34.5360824742268, Blast_Score=123, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17557440, Length=183, Percent_Identity=27.3224043715847, Blast_Score=73, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71988041, Length=179, Percent_Identity=25.1396648044693, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988048, Length=179, Percent_Identity=25.1396648044693, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71984092, Length=103, Percent_Identity=30.0970873786408, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320662, Length=182, Percent_Identity=39.5604395604396, Blast_Score=143, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21356141, Length=198, Percent_Identity=37.3737373737374, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24640542, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45554489, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI116007136, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI161077626, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45554481, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI85724806, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24641280, Length=159, Percent_Identity=29.559748427673, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281360700, Length=159, Percent_Identity=29.559748427673, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24641282, Length=159, Percent_Identity=29.559748427673, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45554818, Length=159, Percent_Identity=29.559748427673, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45554804, Length=159, Percent_Identity=29.559748427673, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45554791, Length=155, Percent_Identity=29.6774193548387, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45554813, Length=155, Percent_Identity=29.6774193548387, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI161077752, Length=155, Percent_Identity=29.6774193548387, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI161077754, Length=155, Percent_Identity=29.6774193548387, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_NITMU (Q2YD10)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_410753.1
- HSSP:   P60546
- ProteinModelPortal:   Q2YD10
- STRING:   Q2YD10
- GeneID:   3786395
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0052
- eggNOG:   COG0194
- HOGENOM:   HBG671982
- OMA:   DIDWQGT
- PhylomeDB:   Q2YD10
- ProtClustDB:   PRK00300
- BioCyc:   NMUL323848:NMUL_A0052-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 23665; Mature: 23534

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPGTLYIISAPSGAGKTSLVKALLQTGIDLSLSISYTSRQPRPEEMDGRDYHFVTRQ
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
VFEQKLQEDEFLESAELYGNFYGTSKKWINETMTSGRDILLEIDSQGARQVRAVFPVAVG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
IFVLPPSLEVLEMRLRQRAQDSLEAISRRLAAAREELSHAGEYNYIIINDKLDRALQDLK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
CIIQAERLKTAKQLVRHHGLLAQLTRTSVL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
TTTPGTLYIISAPSGAGKTSLVKALLQTGIDLSLSISYTSRQPRPEEMDGRDYHFVTRQ
CCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
VFEQKLQEDEFLESAELYGNFYGTSKKWINETMTSGRDILLEIDSQGARQVRAVFPVAVG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
IFVLPPSLEVLEMRLRQRAQDSLEAISRRLAAAREELSHAGEYNYIIINDKLDRALQDLK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
CIIQAERLKTAKQLVRHHGLLAQLTRTSVL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA