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The map label for this gene is insD1 [C]

Identifier: 82545351

GI number: 82545351

Start: 2966025

End: 2966681

Strand: Reverse

Name: insD1 [C]

Synonym: SBO_2957

Alternate gene names: 82545351

Gene position: 2966681-2966025 (Counterclockwise)

Preceding gene: 82545352

Following gene: 82545350

Centisome position: 65.64

GC content: 54.34

Gene sequence:

>657_bases
ATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCTTGAGCGAAAGCCTGCTGTACC
GCCATCGAAACGGGCACATACAGGCAGAGTGGCCGTGAAAGAAAGCAATCAGCGATGGTGCTCTGACGGGTTCGAGTTCT
GCTGTGATAACGGAGAGAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGGTGACT
ACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCAGTGGAACGCCGCTTCGGCAACGATCTTCCGTC
GTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGAC
TTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGAC
TACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATG
GCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACA
GATGTCTGGAAATATAG

Upstream 100 bases:

>100_bases
GGATGTGCTTCTCCGTATACACCATGTTATCGGAGAGCTGCCCACGTATGGTTAGCGTCGGGTATGGGCGCTGCTTCGCA
GACAGGCAGAACTTGATGGT

Downstream 100 bases:

>100_bases
GGGCAAATCCAATGGCGATCAAACGTCATGGCAGTTATCGCATACCTTTTATAATGATTCATTCAGCCATAAAGAACTTG
GCGTAACCGTCGGAGGCCTG

Product: IS2 ORF2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVT
TGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRD
YISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI

Sequences:

>Translated_218_residues
MPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVT
TGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRD
YISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI
>Mature_217_residues
PAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTT
GGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDY
ISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI

Specific function: Involved in the transposition of the insertion sequence is2 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790724, Length=218, Percent_Identity=100, Blast_Score=459, Evalue=1e-131,
Organism=Escherichia coli, GI1789424, Length=218, Percent_Identity=100, Blast_Score=459, Evalue=1e-131,
Organism=Escherichia coli, GI1789224, Length=218, Percent_Identity=100, Blast_Score=459, Evalue=1e-131,
Organism=Escherichia coli, GI1788305, Length=218, Percent_Identity=100, Blast_Score=459, Evalue=1e-131,
Organism=Escherichia coli, GI1787669, Length=218, Percent_Identity=100, Blast_Score=459, Evalue=1e-131,
Organism=Escherichia coli, GI1786558, Length=218, Percent_Identity=100, Blast_Score=459, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 24724; Mature: 24593

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGER
CCCCCHHHHHHHHHHCHHEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCE
LRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCY
EEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCEE
RANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEA
ECHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHH
FEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI
HHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEECC
>Mature Secondary Structure 
PAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGER
CCCCHHHHHHHHHHCHHEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCE
LRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCY
EEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCEE
RANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEA
ECHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHH
FEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI
HHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA