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The map label for this gene is aspS

Identifier: 78780166

GI number: 78780166

Start: 1672586

End: 1674382

Strand: Direct

Name: aspS

Synonym: PMT9312_1781

Alternate gene names: 78780166

Gene position: 1672586-1674382 (Clockwise)

Preceding gene: 78780165

Following gene: 78780167

Centisome position: 97.86

GC content: 33.11

Gene sequence:

>1797_bases
ATGAGAAACAAAATTTGTGATGAACTCAATAATACAGATATTGGTAAATTAGTTAATCTATGTGGATGGGTAGATAGAAG
AAGGGATCATGGTGGTGTAATTTTTATTGATTTAAGAGACCATAGTGGATTCTTACAAATAACAATTAACCCCGATGATG
GAGCAAATCTATTTAAACAGGCAGAAACTCTAAGAAATGAGACGGTAATAATGGTTAGCGGAATTATTAATGAAAGGCCC
AAAGATTCAATAAATAAAAATTTAAGTACTGGAAAGTTAGAGCTTAAGGTTAAAGATTTGCAAATTCTCAACCAAATCAA
AAACAACTTACCTTTTCCAGTTTCTATACATGATTATGAAAATACAAAAGAGGAACTTAGATTAAAATATAGATACCTTG
ATTTAAGAAGGGGAAAATTACTAAAAAATTTAAAAACAAGGCATAAGATTATTAAAGTTGCAAGAGAATTTCTTGATAAT
TTTGGATTTACAGAAGTAGAGACTCCATTACTAACAAAATCAACGCCAGAAGGCGCTCGCGATTTTCTTATTCCTTCACG
TCTTTCGAATGGAGAATTTTTTGCTCTACCTCAATCCCCACAACTATTTAAACAACTTTTAATGGTTGGAGGCTTGGACA
AGTATTATCAAATTGCAAAATGTTTCCGTGATGAAGACTTAAGGGCAGATAGACAGCCGGAGTTTACTCAATTAGATATT
GAGATGAGCTTTATTAGTGAAGAAGAAATAATTTCTTTTAATGAAAGTCTTATAAAAAAAATATGGAAAGAAGTGTTAAA
TATTAATTTTGACAATGCTTTTCCAAGAATGAAATGGCAAGCAGCAATGGATAATTACGGCACTGATAGACCAGATACTA
GATATCAAATGTTACTAAAAGATTTAGGAGAAGTATTAGGTGATATTGGCTTTAATATTTTCACCAAGGCAATTAAGTCT
GGAGGTTCCATAAAATCCATAACAGTCAAAGGAGGTAATTCAAGTATTAGCAACGTAAGAATCAAACCAGGAGGTGATAT
CTTCCAAGTAGCTCAAGATGCAGGAGCTGGTGGTTTGGCCTTTATAAGGGTTAAAGGAGATGAGCTTGAGACTATTGGGG
CAATTAAAAATAATTTAAGTGAAGAGCATATATCTGATATTTTAAGAATCACAGAAGCAGAAGATGGAGACTTAATCCTC
TTGGGAGCTGGAGATAAACAAATTGTCAACCAGTCATTAGATAGAGTGAGGCAATACATCGCAAAAGACTTAAATCTCAT
AGATACAAGTAAATGGAATTTCTTATGGGTAACTGATTTCCCGCTGTTTGAAAGAAATGAAGAGGAAAATAGATATGAAG
CTTTACATCATCCTTTTTGTTCTCCAAAAAATATAAAGTCTAAAGATTCTGAAAACTTGAAAAAAGCAATTGAGAACTCT
ATAGCCAATGCTTATGACTTAGTTCTTAATGGATTGGAGTTAGGAGGTGGCTCTTTACGTATTCATGAAGCGAACTTGCA
ACGAGAGGTTCTGAAAACCGTAGGACTTACTGATAAAGAGATTGATGAAAAATTTGGATTTTTAATAGAAGCCTTAGAAA
TGGGTGCTCCTCCTCATGGTGGAATAGCGTTTGGATTAGATCGTATTACCATGCTGATCATTGGTGCAGATTCAATCAGA
GAAACCATTGCTTTTCCAAAAAATCAACAAGCAAAATGTCTTCTCACAAATGCACCTTCAAATGTCTCTGAATCACAATT
AAAAGAATTGGATATTGAAATAACAATTGATGAATAA

Upstream 100 bases:

>100_bases
AAAGGTTGCATAACAAAAAGATCGTTTTATAAAAAGAATTATTAACTAAATTTACCCTTAAAGAATTATTATAAGTTATT
GATCAATAAATCATACTTAG

Downstream 100 bases:

>100_bases
GAATATATATGGATGTTCTAAAAGTTTTTTGTTTAAATAAAATATAAGGAGGCTGTGCTTAATTAAAAATATTTAATGTC
AAAATTTGTTTTTGTCACCG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MRNKICDELNNTDIGKLVNLCGWVDRRRDHGGVIFIDLRDHSGFLQITINPDDGANLFKQAETLRNETVIMVSGIINERP
KDSINKNLSTGKLELKVKDLQILNQIKNNLPFPVSIHDYENTKEELRLKYRYLDLRRGKLLKNLKTRHKIIKVAREFLDN
FGFTEVETPLLTKSTPEGARDFLIPSRLSNGEFFALPQSPQLFKQLLMVGGLDKYYQIAKCFRDEDLRADRQPEFTQLDI
EMSFISEEEIISFNESLIKKIWKEVLNINFDNAFPRMKWQAAMDNYGTDRPDTRYQMLLKDLGEVLGDIGFNIFTKAIKS
GGSIKSITVKGGNSSISNVRIKPGGDIFQVAQDAGAGGLAFIRVKGDELETIGAIKNNLSEEHISDILRITEAEDGDLIL
LGAGDKQIVNQSLDRVRQYIAKDLNLIDTSKWNFLWVTDFPLFERNEEENRYEALHHPFCSPKNIKSKDSENLKKAIENS
IANAYDLVLNGLELGGGSLRIHEANLQREVLKTVGLTDKEIDEKFGFLIEALEMGAPPHGGIAFGLDRITMLIIGADSIR
ETIAFPKNQQAKCLLTNAPSNVSESQLKELDIEITIDE

Sequences:

>Translated_598_residues
MRNKICDELNNTDIGKLVNLCGWVDRRRDHGGVIFIDLRDHSGFLQITINPDDGANLFKQAETLRNETVIMVSGIINERP
KDSINKNLSTGKLELKVKDLQILNQIKNNLPFPVSIHDYENTKEELRLKYRYLDLRRGKLLKNLKTRHKIIKVAREFLDN
FGFTEVETPLLTKSTPEGARDFLIPSRLSNGEFFALPQSPQLFKQLLMVGGLDKYYQIAKCFRDEDLRADRQPEFTQLDI
EMSFISEEEIISFNESLIKKIWKEVLNINFDNAFPRMKWQAAMDNYGTDRPDTRYQMLLKDLGEVLGDIGFNIFTKAIKS
GGSIKSITVKGGNSSISNVRIKPGGDIFQVAQDAGAGGLAFIRVKGDELETIGAIKNNLSEEHISDILRITEAEDGDLIL
LGAGDKQIVNQSLDRVRQYIAKDLNLIDTSKWNFLWVTDFPLFERNEEENRYEALHHPFCSPKNIKSKDSENLKKAIENS
IANAYDLVLNGLELGGGSLRIHEANLQREVLKTVGLTDKEIDEKFGFLIEALEMGAPPHGGIAFGLDRITMLIIGADSIR
ETIAFPKNQQAKCLLTNAPSNVSESQLKELDIEITIDE
>Mature_598_residues
MRNKICDELNNTDIGKLVNLCGWVDRRRDHGGVIFIDLRDHSGFLQITINPDDGANLFKQAETLRNETVIMVSGIINERP
KDSINKNLSTGKLELKVKDLQILNQIKNNLPFPVSIHDYENTKEELRLKYRYLDLRRGKLLKNLKTRHKIIKVAREFLDN
FGFTEVETPLLTKSTPEGARDFLIPSRLSNGEFFALPQSPQLFKQLLMVGGLDKYYQIAKCFRDEDLRADRQPEFTQLDI
EMSFISEEEIISFNESLIKKIWKEVLNINFDNAFPRMKWQAAMDNYGTDRPDTRYQMLLKDLGEVLGDIGFNIFTKAIKS
GGSIKSITVKGGNSSISNVRIKPGGDIFQVAQDAGAGGLAFIRVKGDELETIGAIKNNLSEEHISDILRITEAEDGDLIL
LGAGDKQIVNQSLDRVRQYIAKDLNLIDTSKWNFLWVTDFPLFERNEEENRYEALHHPFCSPKNIKSKDSENLKKAIENS
IANAYDLVLNGLELGGGSLRIHEANLQREVLKTVGLTDKEIDEKFGFLIEALEMGAPPHGGIAFGLDRITMLIIGADSIR
ETIAFPKNQQAKCLLTNAPSNVSESQLKELDIEITIDE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=607, Percent_Identity=38.8797364085667, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI5031815, Length=265, Percent_Identity=27.5471698113208, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI194272210, Length=265, Percent_Identity=27.5471698113208, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI45439306, Length=242, Percent_Identity=26.8595041322314, Blast_Score=83, Evalue=8e-16,
Organism=Escherichia coli, GI1788173, Length=599, Percent_Identity=47.245409015025, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1789256, Length=291, Percent_Identity=27.8350515463918, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790571, Length=299, Percent_Identity=26.7558528428094, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=605, Percent_Identity=35.5371900826446, Blast_Score=325, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI71994340, Length=255, Percent_Identity=26.2745098039216, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=255, Percent_Identity=26.2745098039216, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=255, Percent_Identity=26.2745098039216, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=309, Percent_Identity=24.2718446601942, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=656, Percent_Identity=33.0792682926829, Blast_Score=286, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6321807, Length=268, Percent_Identity=27.9850746268657, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=261, Percent_Identity=27.2030651340996, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=258, Percent_Identity=25.968992248062, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=153, Percent_Identity=32.0261437908497, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24584738, Length=609, Percent_Identity=37.4384236453202, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17136276, Length=248, Percent_Identity=29.4354838709677, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=27.4509803921569, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640851, Length=255, Percent_Identity=27.4509803921569, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921528, Length=282, Percent_Identity=25.886524822695, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67615; Mature: 67615

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKICDELNNTDIGKLVNLCGWVDRRRDHGGVIFIDLRDHSGFLQITINPDDGANLFKQ
CCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
AETLRNETVIMVSGIINERPKDSINKNLSTGKLELKVKDLQILNQIKNNLPFPVSIHDYE
HHHHCCCEEEEEECCCCCCCCHHHCCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEECCCC
NTKEELRLKYRYLDLRRGKLLKNLKTRHKIIKVAREFLDNFGFTEVETPLLTKSTPEGAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
DFLIPSRLSNGEFFALPQSPQLFKQLLMVGGLDKYYQIAKCFRDEDLRADRQPEFTQLDI
HCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEE
EMSFISEEEIISFNESLIKKIWKEVLNINFDNAFPRMKWQAAMDNYGTDRPDTRYQMLLK
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
DLGEVLGDIGFNIFTKAIKSGGSIKSITVKGGNSSISNVRIKPGGDIFQVAQDAGAGGLA
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCEE
FIRVKGDELETIGAIKNNLSEEHISDILRITEAEDGDLILLGAGDKQIVNQSLDRVRQYI
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHH
AKDLNLIDTSKWNFLWVTDFPLFERNEEENRYEALHHPFCSPKNIKSKDSENLKKAIENS
HHCCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
IANAYDLVLNGLELGGGSLRIHEANLQREVLKTVGLTDKEIDEKFGFLIEALEMGAPPHG
HHHHHHHHHHHHEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
GIAFGLDRITMLIIGADSIRETIAFPKNQQAKCLLTNAPSNVSESQLKELDIEITIDE
CCEECHHHEEEEEECCHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCEEEEECC
>Mature Secondary Structure
MRNKICDELNNTDIGKLVNLCGWVDRRRDHGGVIFIDLRDHSGFLQITINPDDGANLFKQ
CCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
AETLRNETVIMVSGIINERPKDSINKNLSTGKLELKVKDLQILNQIKNNLPFPVSIHDYE
HHHHCCCEEEEEECCCCCCCCHHHCCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEECCCC
NTKEELRLKYRYLDLRRGKLLKNLKTRHKIIKVAREFLDNFGFTEVETPLLTKSTPEGAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
DFLIPSRLSNGEFFALPQSPQLFKQLLMVGGLDKYYQIAKCFRDEDLRADRQPEFTQLDI
HCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEE
EMSFISEEEIISFNESLIKKIWKEVLNINFDNAFPRMKWQAAMDNYGTDRPDTRYQMLLK
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
DLGEVLGDIGFNIFTKAIKSGGSIKSITVKGGNSSISNVRIKPGGDIFQVAQDAGAGGLA
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCEE
FIRVKGDELETIGAIKNNLSEEHISDILRITEAEDGDLILLGAGDKQIVNQSLDRVRQYI
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHH
AKDLNLIDTSKWNFLWVTDFPLFERNEEENRYEALHHPFCSPKNIKSKDSENLKKAIENS
HHCCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
IANAYDLVLNGLELGGGSLRIHEANLQREVLKTVGLTDKEIDEKFGFLIEALEMGAPPHG
HHHHHHHHHHHHEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
GIAFGLDRITMLIIGADSIRETIAFPKNQQAKCLLTNAPSNVSESQLKELDIEITIDE
CCEECHHHEEEEEECCHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA