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The map label for this gene is speB [C]

Identifier: 78780164

GI number: 78780164

Start: 1670486

End: 1671367

Strand: Direct

Name: speB [C]

Synonym: PMT9312_1779

Alternate gene names: 78780164

Gene position: 1670486-1671367 (Clockwise)

Preceding gene: 78780163

Following gene: 78780165

Centisome position: 97.73

GC content: 33.11

Gene sequence:

>882_bases
ATGATAAAAAATTTATTTGATAACGAAAATGCGATTTTTATGGGAGCAAAAAGAAGCCCTAATGATTGCGTAATTGGTAT
CTTTGGAGTCAATTATGATGGGACATGTTCGTTCAAACCAGGAGCTAGATTTGGTCCAGAAGCGATAAGACAAGTCAGTT
CTTGTTTAGAAACATATTGTCCAAAACTCAATAAAGACTTAGAGGATATTATGTATGTTGATTTTGGGTCAATACTAATT
GATAAAAATGACTCAAAGTCCGTTATTGAATCAGTCAAATTAGCAACAAATTTTTTAATTAATAAACGCCTTAGTCCTAT
TATGCTTGGAGGCGAACACTCTATTACAACGGGTGCTATTGAAGCATTAGTAAAAAAATATCCAGATTTGATATTGGTTC
AACTTGATGCTCATGCAGATTTAAGAGAATCATATATAGGAAATCAATATAGTCATGCTTGTGCCATGAAAAGATGCTTA
GAGGTGCTTCCTGAAAAGAAAATTTTGCAAGTAGGAATAAGAAGTGGGACTAAAGAAGAATTTCAAATTATGCATAACAA
CAATCAATTAGTTAACTTTTATCCAGGCGGAAATGCACAAGAGTTAAAAAAAGCTCTTCTACCATACTCTAAGTCTCCAA
TCTATTTAACAATAGATTTAGATTGGTTTGATCCCAGTTTATTAGCAGGGACGGGAACTCCAGAACCGGGAGGATTTTTT
TGGAATGATTTTGAGGAAATCCTGAAAACTTTAAAAGACTTTAGAATTGTGGCTTCAGATATTGTGGAATTATCTCCAGA
AATTGATAAAAGCGGAGTTAGTAGCATAGTTGCAGCCAAAGTACTTAGAAGCTTAATTTTGTCATTAGAAAATATGCAAT
AA

Upstream 100 bases:

>100_bases
CTGAGAATAGAAAAAAGTTGTGAAATTTGGAATTTAAATTTTCAAAATGCAGCATTCAAAATGATGCCAAATAAAATTGT
AAAAGAACTAAATTCATAGA

Downstream 100 bases:

>100_bases
AAAATTTCTAAACTAAAGTGTAAAAATACTAAATTAAAATTAAATATTTCATGGATTTGCTTAAAAGTCCTTTATATGCA
AAATATGTTCAATCCAATGC

Product: arginase family

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MIKNLFDNENAIFMGAKRSPNDCVIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYCPKLNKDLEDIMYVDFGSILI
DKNDSKSVIESVKLATNFLINKRLSPIMLGGEHSITTGAIEALVKKYPDLILVQLDAHADLRESYIGNQYSHACAMKRCL
EVLPEKKILQVGIRSGTKEEFQIMHNNNQLVNFYPGGNAQELKKALLPYSKSPIYLTIDLDWFDPSLLAGTGTPEPGGFF
WNDFEEILKTLKDFRIVASDIVELSPEIDKSGVSSIVAAKVLRSLILSLENMQ

Sequences:

>Translated_293_residues
MIKNLFDNENAIFMGAKRSPNDCVIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYCPKLNKDLEDIMYVDFGSILI
DKNDSKSVIESVKLATNFLINKRLSPIMLGGEHSITTGAIEALVKKYPDLILVQLDAHADLRESYIGNQYSHACAMKRCL
EVLPEKKILQVGIRSGTKEEFQIMHNNNQLVNFYPGGNAQELKKALLPYSKSPIYLTIDLDWFDPSLLAGTGTPEPGGFF
WNDFEEILKTLKDFRIVASDIVELSPEIDKSGVSSIVAAKVLRSLILSLENMQ
>Mature_293_residues
MIKNLFDNENAIFMGAKRSPNDCVIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYCPKLNKDLEDIMYVDFGSILI
DKNDSKSVIESVKLATNFLINKRLSPIMLGGEHSITTGAIEALVKKYPDLILVQLDAHADLRESYIGNQYSHACAMKRCL
EVLPEKKILQVGIRSGTKEEFQIMHNNNQLVNFYPGGNAQELKKALLPYSKSPIYLTIDLDWFDPSLLAGTGTPEPGGFF
WNDFEEILKTLKDFRIVASDIVELSPEIDKSGVSSIVAAKVLRSLILSLENMQ

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=287, Percent_Identity=28.2229965156794, Blast_Score=133, Evalue=1e-31,
Organism=Escherichia coli, GI1789306, Length=250, Percent_Identity=28.8, Blast_Score=97, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6325146, Length=232, Percent_Identity=28.448275862069, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32593; Mature: 32593

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00147 ARGINASE_1 ; PS00148 ARGINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNLFDNENAIFMGAKRSPNDCVIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYC
CCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHC
PKLNKDLEDIMYVDFGSILIDKNDSKSVIESVKLATNFLINKRLSPIMLGGEHSITTGAI
HHCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHH
EALVKKYPDLILVQLDAHADLRESYIGNQYSHACAMKRCLEVLPEKKILQVGIRSGTKEE
HHHHHHCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHH
FQIMHNNNQLVNFYPGGNAQELKKALLPYSKSPIYLTIDLDWFDPSLLAGTGTPEPGGFF
HEEEECCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCEECCCCCCCCCCC
WNDFEEILKTLKDFRIVASDIVELSPEIDKSGVSSIVAAKVLRSLILSLENMQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKNLFDNENAIFMGAKRSPNDCVIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYC
CCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHC
PKLNKDLEDIMYVDFGSILIDKNDSKSVIESVKLATNFLINKRLSPIMLGGEHSITTGAI
HHCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHH
EALVKKYPDLILVQLDAHADLRESYIGNQYSHACAMKRCLEVLPEKKILQVGIRSGTKEE
HHHHHHCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHH
FQIMHNNNQLVNFYPGGNAQELKKALLPYSKSPIYLTIDLDWFDPSLLAGTGTPEPGGFF
HEEEECCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCEECCCCCCCCCCC
WNDFEEILKTLKDFRIVASDIVELSPEIDKSGVSSIVAAKVLRSLILSLENMQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]