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The map label for this gene is speE

Identifier: 78780163

GI number: 78780163

Start: 1669633

End: 1670484

Strand: Direct

Name: speE

Synonym: PMT9312_1778

Alternate gene names: 78780163

Gene position: 1669633-1670484 (Clockwise)

Preceding gene: 78780162

Following gene: 78780164

Centisome position: 97.68

GC content: 30.16

Gene sequence:

>852_bases
ATGACTCATATTTCAACATGGATAGATGAATATCATAAAGGCTCAAGATTCGGCCTAAATGGAGAAATTTTAATTAAACA
AAACTCAAAATATCAAGAAATTATTGTTATTGAAAATGAATATTATGGCAGAGCTTTAATGCTAGATGGGTGTTGGATGA
CATCATTAAAAGACGAAAAATATTATCATGAGTGTCTTGTGCATCCAGCATTAAGTAGCATTGACGAAAAATCTAATGTA
CTAATTATTGGTGGAGGAGACGGTGGTACTGCAAGAGAATGCGTTAAATACCCTCAAATATCAAAAATTGATCTAGTAGA
GATTGATGAAGAAGTAATCAAAATATCTAAAAAATTTTTAAAAGAAATTGGAGGCGAAGCATGGAGTGACAAAAGATTAA
AAATTCATATTGATGATGGTGTTCAATGGGTAAAAAAAACAAGAGATAATTTTTACGACGTTATATTTATAGATTGTTCA
GATCCCTCAGAATTATCAAATTTATTATTTTCAGATTCGTTTTATAGAGAATGCAAAAGAATTCTTACAAAAAATGGTAT
CTTAGCAACGCAAAGCGAATCACCTGAGTCCTTCAAAAATATTCACATAAGTATTTTGAAAACCCTGAAAAAATTCTTTA
GAGTCTCTGAAACTATGTATTCTTTTGTGCCTATATATCCAAGCGGTATTTGGAGTTGGACATTCGCTTCTGAAGAAGTT
CTACATTTATCAAAGCGAAATTGCAATGAAGCCCTGAGAATAGAAAAAAGTTGTGAAATTTGGAATTTAAATTTTCAAAA
TGCAGCATTCAAAATGATGCCAAATAAAATTGTAAAAGAACTAAATTCATAG

Upstream 100 bases:

>100_bases
CATGCAGGAGATAATATTAAGAAACAAACTCCTAAAGATTTTCAACGGCTTTGGCAAATAGCCAAGCAAAAACTTAAAAG
AAAGAATTCTTAAAAAGCAA

Downstream 100 bases:

>100_bases
AATGATAAAAAATTTATTTGATAACGAAAATGCGATTTTTATGGGAGCAAAAAGAAGCCCTAATGATTGCGTAATTGGTA
TCTTTGGAGTCAATTATGAT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTHISTWIDEYHKGSRFGLNGEILIKQNSKYQEIIVIENEYYGRALMLDGCWMTSLKDEKYYHECLVHPALSSIDEKSNV
LIIGGGDGGTARECVKYPQISKIDLVEIDEEVIKISKKFLKEIGGEAWSDKRLKIHIDDGVQWVKKTRDNFYDVIFIDCS
DPSELSNLLFSDSFYRECKRILTKNGILATQSESPESFKNIHISILKTLKKFFRVSETMYSFVPIYPSGIWSWTFASEEV
LHLSKRNCNEALRIEKSCEIWNLNFQNAAFKMMPNKIVKELNS

Sequences:

>Translated_283_residues
MTHISTWIDEYHKGSRFGLNGEILIKQNSKYQEIIVIENEYYGRALMLDGCWMTSLKDEKYYHECLVHPALSSIDEKSNV
LIIGGGDGGTARECVKYPQISKIDLVEIDEEVIKISKKFLKEIGGEAWSDKRLKIHIDDGVQWVKKTRDNFYDVIFIDCS
DPSELSNLLFSDSFYRECKRILTKNGILATQSESPESFKNIHISILKTLKKFFRVSETMYSFVPIYPSGIWSWTFASEEV
LHLSKRNCNEALRIEKSCEIWNLNFQNAAFKMMPNKIVKELNS
>Mature_282_residues
THISTWIDEYHKGSRFGLNGEILIKQNSKYQEIIVIENEYYGRALMLDGCWMTSLKDEKYYHECLVHPALSSIDEKSNVL
IIGGGDGGTARECVKYPQISKIDLVEIDEEVIKISKKFLKEIGGEAWSDKRLKIHIDDGVQWVKKTRDNFYDVIFIDCSD
PSELSNLLFSDSFYRECKRILTKNGILATQSESPESFKNIHISILKTLKKFFRVSETMYSFVPIYPSGIWSWTFASEEVL
HLSKRNCNEALRIEKSCEIWNLNFQNAAFKMMPNKIVKELNS

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=273, Percent_Identity=33.6996336996337, Blast_Score=148, Evalue=4e-36,
Organism=Escherichia coli, GI1786312, Length=282, Percent_Identity=31.5602836879433, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI212645616, Length=227, Percent_Identity=33.920704845815, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25154106, Length=258, Percent_Identity=32.9457364341085, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325326, Length=268, Percent_Identity=34.7014925373134, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323175, Length=287, Percent_Identity=31.3588850174216, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24645443, Length=226, Percent_Identity=35.3982300884956, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI21355941, Length=159, Percent_Identity=36.4779874213836, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_PROM9 (Q317V6)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_398275.1
- ProteinModelPortal:   Q317V6
- SMR:   Q317V6
- STRING:   Q317V6
- GeneID:   3766605
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1778
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   WSWTFAS
- ProtClustDB:   PRK00811
- BioCyc:   PMAR74546:PMT9312_1778-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 32780; Mature: 32649

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 32-32 BINDING 87-87 BINDING 107-107 BINDING 158-158 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHISTWIDEYHKGSRFGLNGEILIKQNSKYQEIIVIENEYYGRALMLDGCWMTSLKDEK
CCCHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCEEEECCCEECCCCCHH
YYHECLVHPALSSIDEKSNVLIIGGGDGGTARECVKYPQISKIDLVEIDEEVIKISKKFL
HHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHH
KEIGGEAWSDKRLKIHIDDGVQWVKKTRDNFYDVIFIDCSDPSELSNLLFSDSFYRECKR
HHHCCCCCCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCHHHHHHHHH
ILTKNGILATQSESPESFKNIHISILKTLKKFFRVSETMYSFVPIYPSGIWSWTFASEEV
HHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCHHH
LHLSKRNCNEALRIEKSCEIWNLNFQNAAFKMMPNKIVKELNS
HHHHHCCCHHHHEECCCCCEEECCCCCHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
THISTWIDEYHKGSRFGLNGEILIKQNSKYQEIIVIENEYYGRALMLDGCWMTSLKDEK
CCHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCEEEECCCEECCCCCHH
YYHECLVHPALSSIDEKSNVLIIGGGDGGTARECVKYPQISKIDLVEIDEEVIKISKKFL
HHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHH
KEIGGEAWSDKRLKIHIDDGVQWVKKTRDNFYDVIFIDCSDPSELSNLLFSDSFYRECKR
HHHCCCCCCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCHHHHHHHHH
ILTKNGILATQSESPESFKNIHISILKTLKKFFRVSETMYSFVPIYPSGIWSWTFASEEV
HHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCHHH
LHLSKRNCNEALRIEKSCEIWNLNFQNAAFKMMPNKIVKELNS
HHHHHCCCHHHHEECCCCCEEECCCCCHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA