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The map label for this gene is dapF

Identifier: 78779296

GI number: 78779296

Start: 845299

End: 846159

Strand: Direct

Name: dapF

Synonym: PMT9312_0912

Alternate gene names: 78779296

Gene position: 845299-846159 (Clockwise)

Preceding gene: 78779294

Following gene: 78779297

Centisome position: 49.46

GC content: 28.69

Gene sequence:

>861_bases
ATGAAAAATATAAATTTTGAAAAATATCAAGGTAACGGAAATGATTTCGTAGTAATTGATTCTAGAGGAAATGATTTATA
TAAAAATTACAAGTCAAATAAAATATTTGATATAAAACAAATTTGCAACAGGCAATTTGGTATTGGAGCAGATGGTGTAA
TTTTTATAGAAGAACCTAATGAAGATAATTATGCAAAAATGATAATCTTTAATTCTGATGGCTCTGAAGCACAAATGTGT
GGAAACGGAATTAGGTGTTTAGTTGAGTATCTCCACGTAAATGATTCAATGAATAATAAAAATATAGAATATAAAATTGA
GACTAAAGCAGGTTTAAAAATTGCAAAATATATAAATGATGAAATTACAGTAAAAATGGGAGTTCCAATTTTAGAAAGTC
AAAATATTCCAACAACAATTGAAAAAAAAATCAATTCAATTCCTTCACACGAATTTATTGAGAAAAATTTCAATAATATG
GGTTATGCCGTAGGAATGGGAAATCCTCATTTAATATTCTTTGTAAAAGACATAGAGTCAATTGTTCTTTACAGACTAGG
TCCTATATTTGAAAAAAATGAATTATTTCCTGAAAAAACTAATGTACATTTTTGTCAAATCATAAATAGAGATAATATCA
AAGTAAAAGTATGGGAAAGAGGTGCAGGACCAACCTTAGCCTGTGGAACAGGTGCCTGTGCTATCCATGTAGCAGCTTAT
AAATTAGGACTTTGTAATCCACAAACCATAGTAACTTTACCAGGAGGTAATCTTAAAATTGATTGGTCAAAAGACGATTT
TGAAGTCATGATGACCGGTAATGCTAAAAAGGTTTTCTCAGGATCAATGTTAGTAAATTAA

Upstream 100 bases:

>100_bases
TTATTTGTTAAATTATTATTTTAATATCCATTGTATAAAATAGAAATAGTTTGTTAAAAGGAATAATTTATAATTAAAAT
TTAAAATATATTATCTACAA

Downstream 100 bases:

>100_bases
TGGAAAATAATTTTATCTATTTAGATAATGCATCCACAACTCCATTATCTAAGAATGTTTTAAATATAATTAATTCAACT
TATAGGAATTATTGGCATAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKNINFEKYQGNGNDFVVIDSRGNDLYKNYKSNKIFDIKQICNRQFGIGADGVIFIEEPNEDNYAKMIIFNSDGSEAQMC
GNGIRCLVEYLHVNDSMNNKNIEYKIETKAGLKIAKYINDEITVKMGVPILESQNIPTTIEKKINSIPSHEFIEKNFNNM
GYAVGMGNPHLIFFVKDIESIVLYRLGPIFEKNELFPEKTNVHFCQIINRDNIKVKVWERGAGPTLACGTGACAIHVAAY
KLGLCNPQTIVTLPGGNLKIDWSKDDFEVMMTGNAKKVFSGSMLVN

Sequences:

>Translated_286_residues
MKNINFEKYQGNGNDFVVIDSRGNDLYKNYKSNKIFDIKQICNRQFGIGADGVIFIEEPNEDNYAKMIIFNSDGSEAQMC
GNGIRCLVEYLHVNDSMNNKNIEYKIETKAGLKIAKYINDEITVKMGVPILESQNIPTTIEKKINSIPSHEFIEKNFNNM
GYAVGMGNPHLIFFVKDIESIVLYRLGPIFEKNELFPEKTNVHFCQIINRDNIKVKVWERGAGPTLACGTGACAIHVAAY
KLGLCNPQTIVTLPGGNLKIDWSKDDFEVMMTGNAKKVFSGSMLVN
>Mature_286_residues
MKNINFEKYQGNGNDFVVIDSRGNDLYKNYKSNKIFDIKQICNRQFGIGADGVIFIEEPNEDNYAKMIIFNSDGSEAQMC
GNGIRCLVEYLHVNDSMNNKNIEYKIETKAGLKIAKYINDEITVKMGVPILESQNIPTTIEKKINSIPSHEFIEKNFNNM
GYAVGMGNPHLIFFVKDIESIVLYRLGPIFEKNELFPEKTNVHFCQIINRDNIKVKVWERGAGPTLACGTGACAIHVAAY
KLGLCNPQTIVTLPGGNLKIDWSKDDFEVMMTGNAKKVFSGSMLVN

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=282, Percent_Identity=32.9787234042553, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 32141; Mature: 32141

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNINFEKYQGNGNDFVVIDSRGNDLYKNYKSNKIFDIKQICNRQFGIGADGVIFIEEPN
CCCCCCEEEECCCCEEEEEECCCCHHHHHCCCCCEEEHHHHCCCCCCCCCCCEEEEECCC
EDNYAKMIIFNSDGSEAQMCGNGIRCLVEYLHVNDSMNNKNIEYKIETKAGLKIAKYIND
CCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCC
EITVKMGVPILESQNIPTTIEKKINSIPSHEFIEKNFNNMGYAVGMGNPHLIFFVKDIES
EEEEEECCCEEECCCCCHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCEEEEEEEHHHH
IVLYRLGPIFEKNELFPEKTNVHFCQIINRDNIKVKVWERGAGPTLACGTGACAIHVAAY
HHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEECCCCEEEEEEEE
KLGLCNPQTIVTLPGGNLKIDWSKDDFEVMMTGNAKKVFSGSMLVN
EEECCCCCEEEEECCCCEEEEECCCCEEEEEECCCCEEECCCEEEC
>Mature Secondary Structure
MKNINFEKYQGNGNDFVVIDSRGNDLYKNYKSNKIFDIKQICNRQFGIGADGVIFIEEPN
CCCCCCEEEECCCCEEEEEECCCCHHHHHCCCCCEEEHHHHCCCCCCCCCCCEEEEECCC
EDNYAKMIIFNSDGSEAQMCGNGIRCLVEYLHVNDSMNNKNIEYKIETKAGLKIAKYIND
CCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCC
EITVKMGVPILESQNIPTTIEKKINSIPSHEFIEKNFNNMGYAVGMGNPHLIFFVKDIES
EEEEEECCCEEECCCCCHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCEEEEEEEHHHH
IVLYRLGPIFEKNELFPEKTNVHFCQIINRDNIKVKVWERGAGPTLACGTGACAIHVAAY
HHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEECCCCEEEEEEEE
KLGLCNPQTIVTLPGGNLKIDWSKDDFEVMMTGNAKKVFSGSMLVN
EEECCCCCEEEEECCCCEEEEECCCCEEEEEECCCCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA