Click here to switch to the map view.

The map label for this gene is iscS [H]

Identifier: 78779297

GI number: 78779297

Start: 846159

End: 847331

Strand: Direct

Name: iscS [H]

Synonym: PMT9312_0913

Alternate gene names: 78779297

Gene position: 846159-847331 (Clockwise)

Preceding gene: 78779296

Following gene: 78779298

Centisome position: 49.51

GC content: 26.26

Gene sequence:

>1173_bases
ATGGAAAATAATTTTATCTATTTAGATAATGCATCCACAACTCCATTATCTAAGAATGTTTTAAATATAATTAATTCAAC
TTATAGGAATTATTGGCATAACCCTTCATCTACATATGAGCTAGGGATAAAATGTTCTACATATCTTGAAAAAATTAGAT
CTAAAATTGCACATATATTTGAAGCAGATCCAGAGGATATAATTTTTACATCTGGTTCTTCGGAATCAACAAATATTGTA
TTTAATAATATTTATGAGAACTTTAAAAATGGTAGAGTTGTTATTTCGAATGTTGAACATCAAGCAACAACTATTTGTGC
TAATAAACTAAGAAAACAAAATTGGGATATTTACGAATGGACGGTAAATAATGATGGAATTTTAAATATTTCTAATATCG
ATGAAATTTTAACAAATGAGACTAAGATTGTATCAATTATTTGGGGACAAAGTGAAATTGGGACAATACAACCGGTTCAA
TTTATAGGTTCCAAATGTGAAGAATTAAATATAATGTTTCATTTAGATGGAACTCAAATATTAAGTAATGGAATATTCAG
TTGGAAAGATCTTAAATGTGATCTTTTAAGTTTATCTGCTCATAAATTTGGAGGTCCAAAAGGTATCGGTATTCTTTTAA
CAAAAGAAAAGTCTAGACAAATTTTAAAAAATAAAGACATATCACTTACTCAGGAATTTTCAATTAGACAAGGTACACAA
GCTTTGCCATTAATCGCTGGAATGTATGAATCATTAAAGAATATTAAAGGAAAAATAAAGTTATGTGATTACATAACTGA
ATTTCCTTCTAATAATATTAATAAACTTAAAAATTATTTTTTTAAAAAAATTAAGGACAATAATCATATAAAGATTACGG
GTAGTATTAACCATAGACTTCCCAATCATATTTCGTTTCTACTATTAAATAAACTATTTGAGCCTATAAGGGCCTATAAG
ATTGTTAATTTCATGTCTGAAAATAAAATAGCCATAAGTAGTGGAAGTGCTTGTTCAAGCTCATCTGGGAAACCTAGCTC
AACACTTAAAAATATTGGTTTAAAAGATGATGAACTATATTCAAATATTCGTGTTACTTTAGGTTCAATAAACAATAAGT
CAGAAATAGATAAATTTTTAGAACTTATTCAAATATGTATTGACAAATTTTAA

Upstream 100 bases:

>100_bases
CAGGAGGTAATCTTAAAATTGATTGGTCAAAAGACGATTTTGAAGTCATGATGACCGGTAATGCTAAAAAGGTTTTCTCA
GGATCAATGTTAGTAAATTA

Downstream 100 bases:

>100_bases
TGGAAACCAATTACAGACTACCTAAAGATTTAAATGAATCTCTTAAGAATATGGAGGATGCAATTATTCCAAGTTTATTA
GATTCAAATAAAAGATTTAC

Product: class-V aminotransferase family cysteine desulfurase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MENNFIYLDNASTTPLSKNVLNIINSTYRNYWHNPSSTYELGIKCSTYLEKIRSKIAHIFEADPEDIIFTSGSSESTNIV
FNNIYENFKNGRVVISNVEHQATTICANKLRKQNWDIYEWTVNNDGILNISNIDEILTNETKIVSIIWGQSEIGTIQPVQ
FIGSKCEELNIMFHLDGTQILSNGIFSWKDLKCDLLSLSAHKFGGPKGIGILLTKEKSRQILKNKDISLTQEFSIRQGTQ
ALPLIAGMYESLKNIKGKIKLCDYITEFPSNNINKLKNYFFKKIKDNNHIKITGSINHRLPNHISFLLLNKLFEPIRAYK
IVNFMSENKIAISSGSACSSSSGKPSSTLKNIGLKDDELYSNIRVTLGSINNKSEIDKFLELIQICIDKF

Sequences:

>Translated_390_residues
MENNFIYLDNASTTPLSKNVLNIINSTYRNYWHNPSSTYELGIKCSTYLEKIRSKIAHIFEADPEDIIFTSGSSESTNIV
FNNIYENFKNGRVVISNVEHQATTICANKLRKQNWDIYEWTVNNDGILNISNIDEILTNETKIVSIIWGQSEIGTIQPVQ
FIGSKCEELNIMFHLDGTQILSNGIFSWKDLKCDLLSLSAHKFGGPKGIGILLTKEKSRQILKNKDISLTQEFSIRQGTQ
ALPLIAGMYESLKNIKGKIKLCDYITEFPSNNINKLKNYFFKKIKDNNHIKITGSINHRLPNHISFLLLNKLFEPIRAYK
IVNFMSENKIAISSGSACSSSSGKPSSTLKNIGLKDDELYSNIRVTLGSINNKSEIDKFLELIQICIDKF
>Mature_390_residues
MENNFIYLDNASTTPLSKNVLNIINSTYRNYWHNPSSTYELGIKCSTYLEKIRSKIAHIFEADPEDIIFTSGSSESTNIV
FNNIYENFKNGRVVISNVEHQATTICANKLRKQNWDIYEWTVNNDGILNISNIDEILTNETKIVSIIWGQSEIGTIQPVQ
FIGSKCEELNIMFHLDGTQILSNGIFSWKDLKCDLLSLSAHKFGGPKGIGILLTKEKSRQILKNKDISLTQEFSIRQGTQ
ALPLIAGMYESLKNIKGKIKLCDYITEFPSNNINKLKNYFFKKIKDNNHIKITGSINHRLPNHISFLLLNKLFEPIRAYK
IVNFMSENKIAISSGSACSSSSGKPSSTLKNIGLKDDELYSNIRVTLGSINNKSEIDKFLELIQICIDKF

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=380, Percent_Identity=28.6842105263158, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI156713448, Length=437, Percent_Identity=24.9427917620137, Blast_Score=125, Evalue=9e-29,
Organism=Escherichia coli, GI48994898, Length=391, Percent_Identity=30.690537084399, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787970, Length=394, Percent_Identity=23.0964467005076, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=387, Percent_Identity=30.2325581395349, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17533177, Length=353, Percent_Identity=25.7790368271955, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=31.5104166666667, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=29.8701298701299, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44212; Mature: 44212

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNFIYLDNASTTPLSKNVLNIINSTYRNYWHNPSSTYELGIKCSTYLEKIRSKIAHIF
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHH
EADPEDIIFTSGSSESTNIVFNNIYENFKNGRVVISNVEHQATTICANKLRKQNWDIYEW
CCCCCCEEEECCCCCCCEEEEHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEE
TVNNDGILNISNIDEILTNETKIVSIIWGQSEIGTIQPVQFIGSKCEELNIMFHLDGTQI
EECCCCEEEECCHHHHHCCCCEEEEEEECCCCCCCCCHHHHHCCCCCEEEEEEEECCHHH
LSNGIFSWKDLKCDLLSLSAHKFGGPKGIGILLTKEKSRQILKNKDISLTQEFSIRQGTQ
HHCCCCCCCCCCEEEEEECHHHCCCCCCEEEEEECHHHHHHHHCCCCCEEEHHHHCCCCC
ALPLIAGMYESLKNIKGKIKLCDYITEFPSNNINKLKNYFFKKIKDNNHIKITGSINHRL
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCC
PNHISFLLLNKLFEPIRAYKIVNFMSENKIAISSGSACSSSSGKPSSTLKNIGLKDDELY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCHHHH
SNIRVTLGSINNKSEIDKFLELIQICIDKF
CCEEEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENNFIYLDNASTTPLSKNVLNIINSTYRNYWHNPSSTYELGIKCSTYLEKIRSKIAHIF
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHH
EADPEDIIFTSGSSESTNIVFNNIYENFKNGRVVISNVEHQATTICANKLRKQNWDIYEW
CCCCCCEEEECCCCCCCEEEEHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEE
TVNNDGILNISNIDEILTNETKIVSIIWGQSEIGTIQPVQFIGSKCEELNIMFHLDGTQI
EECCCCEEEECCHHHHHCCCCEEEEEEECCCCCCCCCHHHHHCCCCCEEEEEEEECCHHH
LSNGIFSWKDLKCDLLSLSAHKFGGPKGIGILLTKEKSRQILKNKDISLTQEFSIRQGTQ
HHCCCCCCCCCCEEEEEECHHHCCCCCCEEEEEECHHHHHHHHCCCCCEEEHHHHCCCCC
ALPLIAGMYESLKNIKGKIKLCDYITEFPSNNINKLKNYFFKKIKDNNHIKITGSINHRL
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCC
PNHISFLLLNKLFEPIRAYKIVNFMSENKIAISSGSACSSSSGKPSSTLKNIGLKDDELY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCHHHH
SNIRVTLGSINNKSEIDKFLELIQICIDKF
CCEEEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA