Click here to switch to the map view.

The map label for this gene is ksgA

Identifier: 78779250

GI number: 78779250

Start: 800675

End: 801505

Strand: Direct

Name: ksgA

Synonym: PMT9312_0866

Alternate gene names: 78779250

Gene position: 800675-801505 (Clockwise)

Preceding gene: 78779244

Following gene: 78779251

Centisome position: 46.84

GC content: 25.27

Gene sequence:

>831_bases
ATGAATTCTAAAAACTATCATCAAAAAAAAAGATTTGGACAACACTGGTTGGTAAATAAAAAAATATTAGAAAAAATTAA
AGAAATTGCTGTTCTTAATGAAAATGACTTTATTTTAGAAATTGGTCCTGGTAAAGGAGCTTTAACCTCTAAGTTGTTAG
ATTCAGAAATTAAAAAATTACATGCAATTGAGTTAGATAAAGATTTAATAAATTTATTAAATGATAAATTCAATAATAAT
GATAAGTTTTCACTGCAGCAGGGAGATATTCTTTCTGTAAATTTAGATTCGATTAATAAGAAGATTACAAAAGTGATTGC
AAATATTCCTTACAATATAACTGGCCCAATATTGGATATTTTCATAGGTCGATTGGGCATTATAAGAAACTATAATTACG
AAAAAATAATATTTTTAATGCAGAAAGATGTTGTAGATAGGATTTTGTCAAAAGAAGGTAGTCCCAATGCTGGTGCGCTT
AGTATAAGAATACAACTTTTATCAAAAATAAAAAGAATATGTGATGTTCCGCCTTCATCATTTAGTCCGCCTCCAAAAGT
TTTTTCTTCTTTAGTAGTTTTCGAACCAATTAAAAATGATTTAAGATTAGATATTAGTCTAGAAAAATATATAGATAAAC
TTCTTCGAATTTCATTTAATTCAAGAAGAAAAATGCTTAGAAATACTCTTAATTCAATACTTTCAAATGAAGAGATAAAT
GAATTATCTGAATCTTCAAAAGTTTGTTTTAATTTAAGACCACAAGATATTTCAATTCACCAATGGATTAAGCTTGCAGA
AAATTGTATTAAAATTAAAAAAAAAATTTAA

Upstream 100 bases:

>100_bases
TTAAATTTATTGAGTTTATCTAAATTTTTTTTTGATAAGCTCATGATATAAAGTATTAAATAAATTCATATTTAATTTAA
ACATATTATTTATCTTTTAG

Downstream 100 bases:

>100_bases
GTATATGCAAGATTTAGCTAAAACGAAAATTAATATAAAATCTCCTGCCAAAATAAATTTGCACCTTGAAGTTATTGGTA
AGAGAGAGGATGGATTTCAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNSKNYHQKKRFGQHWLVNKKILEKIKEIAVLNENDFILEIGPGKGALTSKLLDSEIKKLHAIELDKDLINLLNDKFNNN
DKFSLQQGDILSVNLDSINKKITKVIANIPYNITGPILDIFIGRLGIIRNYNYEKIIFLMQKDVVDRILSKEGSPNAGAL
SIRIQLLSKIKRICDVPPSSFSPPPKVFSSLVVFEPIKNDLRLDISLEKYIDKLLRISFNSRRKMLRNTLNSILSNEEIN
ELSESSKVCFNLRPQDISIHQWIKLAENCIKIKKKI

Sequences:

>Translated_276_residues
MNSKNYHQKKRFGQHWLVNKKILEKIKEIAVLNENDFILEIGPGKGALTSKLLDSEIKKLHAIELDKDLINLLNDKFNNN
DKFSLQQGDILSVNLDSINKKITKVIANIPYNITGPILDIFIGRLGIIRNYNYEKIIFLMQKDVVDRILSKEGSPNAGAL
SIRIQLLSKIKRICDVPPSSFSPPPKVFSSLVVFEPIKNDLRLDISLEKYIDKLLRISFNSRRKMLRNTLNSILSNEEIN
ELSESSKVCFNLRPQDISIHQWIKLAENCIKIKKKI
>Mature_276_residues
MNSKNYHQKKRFGQHWLVNKKILEKIKEIAVLNENDFILEIGPGKGALTSKLLDSEIKKLHAIELDKDLINLLNDKFNNN
DKFSLQQGDILSVNLDSINKKITKVIANIPYNITGPILDIFIGRLGIIRNYNYEKIIFLMQKDVVDRILSKEGSPNAGAL
SIRIQLLSKIKRICDVPPSSFSPPPKVFSSLVVFEPIKNDLRLDISLEKYIDKLLRISFNSRRKMLRNTLNSILSNEEIN
ELSESSKVCFNLRPQDISIHQWIKLAENCIKIKKKI

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=235, Percent_Identity=33.1914893617021, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI156415992, Length=282, Percent_Identity=23.4042553191489, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=28.735632183908, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25146882, Length=243, Percent_Identity=30.4526748971193, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25141369, Length=291, Percent_Identity=26.8041237113402, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=212, Percent_Identity=29.7169811320755, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21358017, Length=236, Percent_Identity=32.2033898305085, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357273, Length=277, Percent_Identity=25.6317689530686, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PROM9 (Q31B19)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397362.1
- ProteinModelPortal:   Q31B19
- SMR:   Q31B19
- STRING:   Q31B19
- GeneID:   3765667
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0866
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   PMAR74546:PMT9312_0866-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31706; Mature: 31706

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 15-15 BINDING 17-17 BINDING 42-42 BINDING 64-64 BINDING 89-89 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKNYHQKKRFGQHWLVNKKILEKIKEIAVLNENDFILEIGPGKGALTSKLLDSEIKKL
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHH
HAIELDKDLINLLNDKFNNNDKFSLQQGDILSVNLDSINKKITKVIANIPYNITGPILDI
HHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHH
FIGRLGIIRNYNYEKIIFLMQKDVVDRILSKEGSPNAGALSIRIQLLSKIKRICDVPPSS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
FSPPPKVFSSLVVFEPIKNDLRLDISLEKYIDKLLRISFNSRRKMLRNTLNSILSNEEIN
CCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHH
ELSESSKVCFNLRPQDISIHQWIKLAENCIKIKKKI
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSKNYHQKKRFGQHWLVNKKILEKIKEIAVLNENDFILEIGPGKGALTSKLLDSEIKKL
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHH
HAIELDKDLINLLNDKFNNNDKFSLQQGDILSVNLDSINKKITKVIANIPYNITGPILDI
HHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHH
FIGRLGIIRNYNYEKIIFLMQKDVVDRILSKEGSPNAGALSIRIQLLSKIKRICDVPPSS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
FSPPPKVFSSLVVFEPIKNDLRLDISLEKYIDKLLRISFNSRRKMLRNTLNSILSNEEIN
CCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHH
ELSESSKVCFNLRPQDISIHQWIKLAENCIKIKKKI
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA