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The map label for this gene is ispE [H]

Identifier: 78779251

GI number: 78779251

Start: 801510

End: 802445

Strand: Direct

Name: ispE [H]

Synonym: PMT9312_0867

Alternate gene names: 78779251

Gene position: 801510-802445 (Clockwise)

Preceding gene: 78779250

Following gene: 78779252

Centisome position: 46.89

GC content: 27.88

Gene sequence:

>936_bases
ATGCAAGATTTAGCTAAAACGAAAATTAATATAAAATCTCCTGCCAAAATAAATTTGCACCTTGAAGTTATTGGTAAGAG
AGAGGATGGATTTCATGAGTTAGCAATGATTATGCAAAATATCGATCTTTCTGATTATTTAGAATTTGAAATTAATAATG
AAGGTTTAATTAAACTTGAGTCTGATTGTAATGATTTAAGCTTATCTGATGATAACTTAATTGTTAAATCGGCAAATCTA
TTAAGGAAAAAATCAAATATAGATTACGGTGCGAATATATTTTTAAGAAAAAATATTCCAATTGGCGCAGGATTAGCTGG
TGGATCCAGTAATTCAGCAGCAACATTAATTGGTCTTAATAAGTTATGGGATTTGAACTTAGGTCAAGAAGCATTATGTT
CATTAGCATCAACTTTAGGATCTGATATTCCCTTTTTTATAAATGGTGGTATTCAATTATGTTTTGGAAGAGGAGAAGTT
TTGGAGAAATTAGATTCAAACTTTGAATATGGAGTAATTCTTTTAAAAAATCCAAATGTATCAGTATCTACAGCTGAAAC
TTATAAAAAATATAGTAATAGATTTTGTCATCAATACCTTACTGATAGAGAAATGATTGAGAACATAAGAAAAAATTTAA
GAGAAAATGGTTTAAATAACTTAAATCTTGATAATCAACATTTATCTATTAAAAATGATTTGCAGTTAGTTGTTGAAAAT
GAAAATGATTCTGTAAAGCAGGCATTATATTTACTTTCTAAATTAGAAAATTGTCTCACATTTTCAATGAGTGGATCAGG
TCCTACATGCTTTGCACTCTTTAAAGATATAGAGACTGCTAAAAAAGAATTAACTGCGAATTATAAATTGTTTAGAAATA
AAGGTTACGATTCATGGGTTTGCACTTTCCTTGAAAAGGGAATAACATTCATTTAA

Upstream 100 bases:

>100_bases
TCAAAAGTTTGTTTTAATTTAAGACCACAAGATATTTCAATTCACCAATGGATTAAGCTTGCAGAAAATTGTATTAAAAT
TAAAAAAAAAATTTAAGTAT

Downstream 100 bases:

>100_bases
AAATTTTTTTATACAAAATTCTATTGTGGCTGATAATAGTAATGAGAATATTGAAAAAAACATTCCCGAAAAAGGACCAT
TAAATTTTATTGTAGGATCA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MQDLAKTKINIKSPAKINLHLEVIGKREDGFHELAMIMQNIDLSDYLEFEINNEGLIKLESDCNDLSLSDDNLIVKSANL
LRKKSNIDYGANIFLRKNIPIGAGLAGGSSNSAATLIGLNKLWDLNLGQEALCSLASTLGSDIPFFINGGIQLCFGRGEV
LEKLDSNFEYGVILLKNPNVSVSTAETYKKYSNRFCHQYLTDREMIENIRKNLRENGLNNLNLDNQHLSIKNDLQLVVEN
ENDSVKQALYLLSKLENCLTFSMSGSGPTCFALFKDIETAKKELTANYKLFRNKGYDSWVCTFLEKGITFI

Sequences:

>Translated_311_residues
MQDLAKTKINIKSPAKINLHLEVIGKREDGFHELAMIMQNIDLSDYLEFEINNEGLIKLESDCNDLSLSDDNLIVKSANL
LRKKSNIDYGANIFLRKNIPIGAGLAGGSSNSAATLIGLNKLWDLNLGQEALCSLASTLGSDIPFFINGGIQLCFGRGEV
LEKLDSNFEYGVILLKNPNVSVSTAETYKKYSNRFCHQYLTDREMIENIRKNLRENGLNNLNLDNQHLSIKNDLQLVVEN
ENDSVKQALYLLSKLENCLTFSMSGSGPTCFALFKDIETAKKELTANYKLFRNKGYDSWVCTFLEKGITFI
>Mature_311_residues
MQDLAKTKINIKSPAKINLHLEVIGKREDGFHELAMIMQNIDLSDYLEFEINNEGLIKLESDCNDLSLSDDNLIVKSANL
LRKKSNIDYGANIFLRKNIPIGAGLAGGSSNSAATLIGLNKLWDLNLGQEALCSLASTLGSDIPFFINGGIQLCFGRGEV
LEKLDSNFEYGVILLKNPNVSVSTAETYKKYSNRFCHQYLTDREMIENIRKNLRENGLNNLNLDNQHLSIKNDLQLVVEN
ENDSVKQALYLLSKLENCLTFSMSGSGPTCFALFKDIETAKKELTANYKLFRNKGYDSWVCTFLEKGITFI

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=307, Percent_Identity=30.6188925081433, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 34839; Mature: 34839

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLAKTKINIKSPAKINLHLEVIGKREDGFHELAMIMQNIDLSDYLEFEINNEGLIKLE
CCCHHHCEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEC
SDCNDLSLSDDNLIVKSANLLRKKSNIDYGANIFLRKNIPIGAGLAGGSSNSAATLIGLN
CCCCCCCCCCCCEEEECHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECC
KLWDLNLGQEALCSLASTLGSDIPFFINGGIQLCFGRGEVLEKLDSNFEYGVILLKNPNV
HHEECCCCHHHHHHHHHHHCCCCCEEEECCEEEEECCHHHHHHHCCCCCEEEEEEECCCC
SVSTAETYKKYSNRFCHQYLTDREMIENIRKNLRENGLNNLNLDNQHLSIKNDLQLVVEN
CEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEC
ENDSVKQALYLLSKLENCLTFSMSGSGPTCFALFKDIETAKKELTANYKLFRNKGYDSWV
CCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHEEECCCCCHHH
CTFLEKGITFI
HHHHHCCCCCC
>Mature Secondary Structure
MQDLAKTKINIKSPAKINLHLEVIGKREDGFHELAMIMQNIDLSDYLEFEINNEGLIKLE
CCCHHHCEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEC
SDCNDLSLSDDNLIVKSANLLRKKSNIDYGANIFLRKNIPIGAGLAGGSSNSAATLIGLN
CCCCCCCCCCCCEEEECHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECC
KLWDLNLGQEALCSLASTLGSDIPFFINGGIQLCFGRGEVLEKLDSNFEYGVILLKNPNV
HHEECCCCHHHHHHHHHHHCCCCCEEEECCEEEEECCHHHHHHHCCCCCEEEEEEECCCC
SVSTAETYKKYSNRFCHQYLTDREMIENIRKNLRENGLNNLNLDNQHLSIKNDLQLVVEN
CEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEC
ENDSVKQALYLLSKLENCLTFSMSGSGPTCFALFKDIETAKKELTANYKLFRNKGYDSWV
CCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHEEECCCCCHHH
CTFLEKGITFI
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA