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The map label for this gene is phaD [H]

Identifier: 78484407

GI number: 78484407

Start: 72315

End: 73841

Strand: Reverse

Name: phaD [H]

Synonym: Tcr_0061

Alternate gene names: 78484407

Gene position: 73841-72315 (Counterclockwise)

Preceding gene: 78484408

Following gene: 78484406

Centisome position: 3.04

GC content: 44.34

Gene sequence:

>1527_bases
ATGATGTCATACCCTTTTTTACCCGTTTTAATCCCTTTAATTGGCGGCGTCCTTGTTTTATTGGTACGCCAAGCCGGGCT
AAATTTGCAACGTTTGTTAAGTTTTTTATTGGTTCTGGCACTGCTTGCTGTCAGCGTGGAACTCATGTTTGTCGCCAGCC
AGGGATCACCTCAAGTGTATGCATTAGGTAACTGGGTGGCGCCATATGGCATCGTTTTAGTGTTAGACCAACTGTCCGCT
TTAATGATTTTAATTACAGCCATTTTAGCGGTAGGTGCTTTATGGTATGCCATTTCCACAAAAATGGATGGACAAGGATC
CCATTTCCATGTGTTATTTCAAATTCAACTGTTTGGCTTAAACGGCGCCTTTATGACAGGAGATGTCTTTAACCTGTTTG
TTTTCTTTGAGGTATTGTTACTGGCCTCTTATGGTTTGATGCTTCATGGCGGAGGGCGTTTGAGAACCAAAGCGGGCTTA
CAATATGTTGTGATCAATTTGATTGGATCCACACTTTTCTTATTTGCCGTAGGCGCGCTTTATGGTGTGTTAGGCACGTT
GAACATTGCCGATATGGCCAATAAAGTGGCTGTCCTTTCAACAGAAGATCAAGGTGTCGTTGCAGCGGCCGGTTTATTAT
TGTTACTGGTTTTTGGTATTAAAGCGGCTATGTTTCCGCTGTATTTATGGCTTCCTCAAGCGTATTCAAATGCCGCTGCT
CCAGTCGCCGCTTTGTTTGCAATCATGACCAAAGTCGGGTTGTATGCCATTATTCGTGTCCACGGCACGGTGTTTGGTGA
AAATGCACAAGAATTGGCTTTTTTCTACACGCCTTGGTTACTCGGGCTGGGTCTGATAACCATCGTTCTAGCGGCATTAG
GCGTCATGGCCTCACGAGGTTTAAGAGAACAGGTAGCTTATCTGGTATTGGCCTCCATTGCCACACTCTTGATCGGGATA
GGACTCAATACGCCGGCCGCTTTATCCGCCAGTTTGTACTATTTAATCCATTCCACTCTGATTGCAGGTGGTTTCTTCTT
ATTAGCCGATATCATCGCCCGAGGCAAAGGTTCCTTAGAAGATCACTTTAAACCCGGCCCGGCCGTACCACAGGCCATTC
TGATCGGCTCGACTTTTATCTTTGCGGCTATCGCTATGGTGGGAATGCCGCCTTTATCGGGCTTCTTTGGAAAAGTTCTC
ATTCTTTCAGCCGCTTTGAACCATGATTACATTGGCTGGATCTTAACCTTAGTCCTTATATCGAGCTTAATAATGATTAT
TGCCATGGCTCGTTCCGGTTCCGTTTTGTTTTATCATATTCAACCCAGTACGGAGTCAACAGGAGAACCTCTGAATAAAT
TAGCTTATTTAGCCGTCATTTTATTGTTTTCAACCAGTGTGGTATTAGTGGTATTTGCCCAACCTATTACCTCAATGACC
GATACCATTGCACAACAATTATTTGACTCGAATCATTACATTCATTCGGTTCTTAATACCCCTGCTGTTGGAGAGGTGTC
GCCATGA

Upstream 100 bases:

>100_bases
ATCATGTGGACGGCTTACATCTAGCCTCCGATGAAGTCGCTTATGATAAAAAATCGACTGCGCAGAAAACGCAGCAACCC
AATAAAGAGGCCTCCCACGA

Downstream 100 bases:

>100_bases
ACATTAAAAAAATCTTACCTCATCCCATCTTAAGTTTATTTTTATGGGGAATTTGGTTATTACTGAACAACAATTTTAGC
GCAGGCCATATTGTGCTTGG

Product: NADH dehydrogenase (quinone)

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MMSYPFLPVLIPLIGGVLVLLVRQAGLNLQRLLSFLLVLALLAVSVELMFVASQGSPQVYALGNWVAPYGIVLVLDQLSA
LMILITAILAVGALWYAISTKMDGQGSHFHVLFQIQLFGLNGAFMTGDVFNLFVFFEVLLLASYGLMLHGGGRLRTKAGL
QYVVINLIGSTLFLFAVGALYGVLGTLNIADMANKVAVLSTEDQGVVAAAGLLLLLVFGIKAAMFPLYLWLPQAYSNAAA
PVAALFAIMTKVGLYAIIRVHGTVFGENAQELAFFYTPWLLGLGLITIVLAALGVMASRGLREQVAYLVLASIATLLIGI
GLNTPAALSASLYYLIHSTLIAGGFFLLADIIARGKGSLEDHFKPGPAVPQAILIGSTFIFAAIAMVGMPPLSGFFGKVL
ILSAALNHDYIGWILTLVLISSLIMIIAMARSGSVLFYHIQPSTESTGEPLNKLAYLAVILLFSTSVVLVVFAQPITSMT
DTIAQQLFDSNHYIHSVLNTPAVGEVSP

Sequences:

>Translated_508_residues
MMSYPFLPVLIPLIGGVLVLLVRQAGLNLQRLLSFLLVLALLAVSVELMFVASQGSPQVYALGNWVAPYGIVLVLDQLSA
LMILITAILAVGALWYAISTKMDGQGSHFHVLFQIQLFGLNGAFMTGDVFNLFVFFEVLLLASYGLMLHGGGRLRTKAGL
QYVVINLIGSTLFLFAVGALYGVLGTLNIADMANKVAVLSTEDQGVVAAAGLLLLLVFGIKAAMFPLYLWLPQAYSNAAA
PVAALFAIMTKVGLYAIIRVHGTVFGENAQELAFFYTPWLLGLGLITIVLAALGVMASRGLREQVAYLVLASIATLLIGI
GLNTPAALSASLYYLIHSTLIAGGFFLLADIIARGKGSLEDHFKPGPAVPQAILIGSTFIFAAIAMVGMPPLSGFFGKVL
ILSAALNHDYIGWILTLVLISSLIMIIAMARSGSVLFYHIQPSTESTGEPLNKLAYLAVILLFSTSVVLVVFAQPITSMT
DTIAQQLFDSNHYIHSVLNTPAVGEVSP
>Mature_508_residues
MMSYPFLPVLIPLIGGVLVLLVRQAGLNLQRLLSFLLVLALLAVSVELMFVASQGSPQVYALGNWVAPYGIVLVLDQLSA
LMILITAILAVGALWYAISTKMDGQGSHFHVLFQIQLFGLNGAFMTGDVFNLFVFFEVLLLASYGLMLHGGGRLRTKAGL
QYVVINLIGSTLFLFAVGALYGVLGTLNIADMANKVAVLSTEDQGVVAAAGLLLLLVFGIKAAMFPLYLWLPQAYSNAAA
PVAALFAIMTKVGLYAIIRVHGTVFGENAQELAFFYTPWLLGLGLITIVLAALGVMASRGLREQVAYLVLASIATLLIGI
GLNTPAALSASLYYLIHSTLIAGGFFLLADIIARGKGSLEDHFKPGPAVPQAILIGSTFIFAAIAMVGMPPLSGFFGKVL
ILSAALNHDYIGWILTLVLISSLIMIIAMARSGSVLFYHIQPSTESTGEPLNKLAYLAVILLFSTSVVLVVFAQPITSMT
DTIAQQLFDSNHYIHSVLNTPAVGEVSP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=385, Percent_Identity=25.974025974026, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1788613, Length=428, Percent_Identity=27.5700934579439, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788827, Length=344, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788831, Length=392, Percent_Identity=22.4489795918367, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788829, Length=386, Percent_Identity=24.8704663212435, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788614, Length=261, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 54078; Mature: 54078

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSYPFLPVLIPLIGGVLVLLVRQAGLNLQRLLSFLLVLALLAVSVELMFVASQGSPQVY
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEE
ALGNWVAPYGIVLVLDQLSALMILITAILAVGALWYAISTKMDGQGSHFHVLFQIQLFGL
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEC
NGAFMTGDVFNLFVFFEVLLLASYGLMLHGGGRLRTKAGLQYVVINLIGSTLFLFAVGAL
CCCEECHHHHHHHHHHHHHHHHHCCCEEECCCCEEHHHCHHHHHHHHHHHHHHHHHHHHH
YGVLGTLNIADMANKVAVLSTEDQGVVAAAGLLLLLVFGIKAAMFPLYLWLPQAYSNAAA
HHHHHCCCHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
PVAALFAIMTKVGLYAIIRVHGTVFGENAQELAFFYTPWLLGLGLITIVLAALGVMASRG
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LREQVAYLVLASIATLLIGIGLNTPAALSASLYYLIHSTLIAGGFFLLADIIARGKGSLE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
DHFKPGPAVPQAILIGSTFIFAAIAMVGMPPLSGFFGKVLILSAALNHDYIGWILTLVLI
HCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
SSLIMIIAMARSGSVLFYHIQPSTESTGEPLNKLAYLAVILLFSTSVVLVVFAQPITSMT
HHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTIAQQLFDSNHYIHSVLNTPAVGEVSP
HHHHHHHHCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMSYPFLPVLIPLIGGVLVLLVRQAGLNLQRLLSFLLVLALLAVSVELMFVASQGSPQVY
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEE
ALGNWVAPYGIVLVLDQLSALMILITAILAVGALWYAISTKMDGQGSHFHVLFQIQLFGL
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEC
NGAFMTGDVFNLFVFFEVLLLASYGLMLHGGGRLRTKAGLQYVVINLIGSTLFLFAVGAL
CCCEECHHHHHHHHHHHHHHHHHCCCEEECCCCEEHHHCHHHHHHHHHHHHHHHHHHHHH
YGVLGTLNIADMANKVAVLSTEDQGVVAAAGLLLLLVFGIKAAMFPLYLWLPQAYSNAAA
HHHHHCCCHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
PVAALFAIMTKVGLYAIIRVHGTVFGENAQELAFFYTPWLLGLGLITIVLAALGVMASRG
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LREQVAYLVLASIATLLIGIGLNTPAALSASLYYLIHSTLIAGGFFLLADIIARGKGSLE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
DHFKPGPAVPQAILIGSTFIFAAIAMVGMPPLSGFFGKVLILSAALNHDYIGWILTLVLI
HCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
SSLIMIIAMARSGSVLFYHIQPSTESTGEPLNKLAYLAVILLFSTSVVLVVFAQPITSMT
HHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTIAQQLFDSNHYIHSVLNTPAVGEVSP
HHHHHHHHCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]