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The map label for this gene is hynB2 [H]

Identifier: 78358794

GI number: 78358794

Start: 3706011

End: 3706976

Strand: Direct

Name: hynB2 [H]

Synonym: Dde_3755

Alternate gene names: 78358794

Gene position: 3706011-3706976 (Clockwise)

Preceding gene: 78358793

Following gene: 78358795

Centisome position: 99.35

GC content: 56.42

Gene sequence:

>966_bases
ATGCCCAACGGTAACAGATTTGATGCACTGAAAATGACCGTAGGAACCAGAGAGGTCAGCCGCCGTGATTTTATGAAGTT
CTGCGGTGTCATGGCTACTTTTCTGGGCATGGGGCCCGCATTTGCCCCGCAGATAGCCCATGCGCTGATGACCAAAAAAC
GCCCTTCGGTGGTGTACCTGCACTGCGCGGAATGCACCGGCTGCACAGAAGGACTGCTCAGAGCGTATGAGCCGTATTTT
GACGAAATAATCATGAACACCATCTCGCTGGACTATTGCGAGACAGTCATGGCTGCCGCAGGCGATGCAGCCCACGCAGC
GCTGGAAAAAGCCCTCATCAATCCGGAAGGATATATCTGCGTCATCGAAGGCGGCATACCCACCCGTCATGGCGGAGAAT
ACGGCAAAGTGGGCGGAGAAACCATGTTTCAGCTGTGCGCGCGCGTGGCCTCCAAAGCCATGGCCACCATCGCCATGGGG
TCTTGTGCCTGTTTTGGCGGTGTGCAGGCTGCCGCGCCCAATCCTTCGGGAGCCAAAGGGGTGAACGAAGCCCTGAAAGC
AGTGGGGGTGAATGCCATCAACATTGCCGGTTGCCCGCCCAACCCCATGAACTTTGTGGGCACAGTGGTGCACCTGCTTA
CCAAGGGTATGCCTGAACTTGATACCTGGAACCGCCCGCTGCTGTTTTTCGGTGATACCGTGCACGATCACTGCCCGCGG
CAGAAGCATTTTAACAAAGGCGAATTTGCCCCGGGCTTTGGCAGTCCCGAAGCCAAAAAAGGCTGGTGCCTGTATCAGCT
GGGCTGCAAAGGGCCGTACACCTATAACAACTGCCCCACAGCGCTTTTCAATCAGGTCAACTGGCCGGTAAAGGCCGGAG
CCCCCTGTATAGGCTGCAGCGAACCCAACTTCTGGGACAAATACTCGCCCTTTTTCCACCCCATTGAAGACGGCCCCGAA
AGCTAA

Upstream 100 bases:

>100_bases
CTGTCTGGGCTGTCACACGCTGATGCGCAGCAAAGGCGGCAAAAATCCGGTGGGCTGCACCTCTGCCTGCCACAAGCCCC
GGTAGCCACAGGAGGATATC

Downstream 100 bases:

>100_bases
CCCGCGCCGCCCGAAAGGAGATAAGACCGAATGAGTCAGAAAAAAATGAACCACACCGGCAAAAGGCTGATCGTCGATCC
GGTGACGCGGATCGAAGGGC

Product: hydrogenase (NiFe) small subunit (hydA)

Products: NA

Alternate protein names: NiFe hydrogenlyase small chain 2 [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MPNGNRFDALKMTVGTREVSRRDFMKFCGVMATFLGMGPAFAPQIAHALMTKKRPSVVYLHCAECTGCTEGLLRAYEPYF
DEIIMNTISLDYCETVMAAAGDAAHAALEKALINPEGYICVIEGGIPTRHGGEYGKVGGETMFQLCARVASKAMATIAMG
SCACFGGVQAAAPNPSGAKGVNEALKAVGVNAINIAGCPPNPMNFVGTVVHLLTKGMPELDTWNRPLLFFGDTVHDHCPR
QKHFNKGEFAPGFGSPEAKKGWCLYQLGCKGPYTYNNCPTALFNQVNWPVKAGAPCIGCSEPNFWDKYSPFFHPIEDGPE
S

Sequences:

>Translated_321_residues
MPNGNRFDALKMTVGTREVSRRDFMKFCGVMATFLGMGPAFAPQIAHALMTKKRPSVVYLHCAECTGCTEGLLRAYEPYF
DEIIMNTISLDYCETVMAAAGDAAHAALEKALINPEGYICVIEGGIPTRHGGEYGKVGGETMFQLCARVASKAMATIAMG
SCACFGGVQAAAPNPSGAKGVNEALKAVGVNAINIAGCPPNPMNFVGTVVHLLTKGMPELDTWNRPLLFFGDTVHDHCPR
QKHFNKGEFAPGFGSPEAKKGWCLYQLGCKGPYTYNNCPTALFNQVNWPVKAGAPCIGCSEPNFWDKYSPFFHPIEDGPE
S
>Mature_320_residues
PNGNRFDALKMTVGTREVSRRDFMKFCGVMATFLGMGPAFAPQIAHALMTKKRPSVVYLHCAECTGCTEGLLRAYEPYFD
EIIMNTISLDYCETVMAAAGDAAHAALEKALINPEGYICVIEGGIPTRHGGEYGKVGGETMFQLCARVASKAMATIAMGS
CACFGGVQAAAPNPSGAKGVNEALKAVGVNAINIAGCPPNPMNFVGTVVHLLTKGMPELDTWNRPLLFFGDTVHDHCPRQ
KHFNKGEFAPGFGSPEAKKGWCLYQLGCKGPYTYNNCPTALFNQVNWPVKAGAPCIGCSEPNFWDKYSPFFHPIEDGPES

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=287, Percent_Identity=44.9477351916376, Blast_Score=241, Evalue=4e-65,
Organism=Escherichia coli, GI1787206, Length=305, Percent_Identity=40.327868852459, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 34565; Mature: 34434

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNGNRFDALKMTVGTREVSRRDFMKFCGVMATFLGMGPAFAPQIAHALMTKKRPSVVYL
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEE
HCAECTGCTEGLLRAYEPYFDEIIMNTISLDYCETVMAAAGDAAHAALEKALINPEGYIC
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEE
VIEGGIPTRHGGEYGKVGGETMFQLCARVASKAMATIAMGSCACFGGVQAAAPNPSGAKG
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHH
VNEALKAVGVNAINIAGCPPNPMNFVGTVVHLLTKGMPELDTWNRPLLFFGDTVHDHCPR
HHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHCCC
QKHFNKGEFAPGFGSPEAKKGWCLYQLGCKGPYTYNNCPTALFNQVNWPVKAGAPCIGCS
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCHHCCCCEECCC
EPNFWDKYSPFFHPIEDGPES
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PNGNRFDALKMTVGTREVSRRDFMKFCGVMATFLGMGPAFAPQIAHALMTKKRPSVVYL
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEE
HCAECTGCTEGLLRAYEPYFDEIIMNTISLDYCETVMAAAGDAAHAALEKALINPEGYIC
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEE
VIEGGIPTRHGGEYGKVGGETMFQLCARVASKAMATIAMGSCACFGGVQAAAPNPSGAKG
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHH
VNEALKAVGVNAINIAGCPPNPMNFVGTVVHLLTKGMPELDTWNRPLLFFGDTVHDHCPR
HHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHCCC
QKHFNKGEFAPGFGSPEAKKGWCLYQLGCKGPYTYNNCPTALFNQVNWPVKAGAPCIGCS
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCHHCCCCEECCC
EPNFWDKYSPFFHPIEDGPES
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA