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The map label for this gene is hydB [H]

Identifier: 78358795

GI number: 78358795

Start: 3707007

End: 3708671

Strand: Direct

Name: hydB [H]

Synonym: Dde_3756

Alternate gene names: 78358795

Gene position: 3707007-3708671 (Clockwise)

Preceding gene: 78358794

Following gene: 78358797

Centisome position: 99.38

GC content: 54.83

Gene sequence:

>1665_bases
ATGAGTCAGAAAAAAATGAACCACACCGGCAAAAGGCTGATCGTCGATCCGGTGACGCGGATCGAAGGGCACCTCAAGAT
CGAGGTGGAACTGGAAAACAACAAAGTCAAAGATGCATGGGTAAGCACACAGCTTTTCAGAGGCATTGAGATGATTCTGA
AAGGCAGACCGCCGGAAGATGCCCCGCTGTTTACCCAGCGTGCTTGCGGCGTATGCACAAACACCCATGCGCTGACCAGC
ATCCGCGCCATCGAAGACGCACTTGAGATCAAAGTCCCTCCGCTGGCACAGCTCATGCGCCACCTTATTCTCTCCGCGCT
TATCGTGCATGACCATCTGGTGCACTTTTACCACCTGCACGGGCTGGACTGGATTGATGTTGCCGCAGCCACCACGGCCG
ATCCGGCCAAGGCCGGTAAAATTGTGGGCAGCACCAGCAACAGAAGCGACGACCCTTCAGAGCTTTTCATCATTCAGAAG
CGGCTGAAGGAGTTTGTGAAATCGGGACAGCTGGGATTTCTGGAAAAGGCATATTTTCTGGGCGGCAACGATGCGTACAA
GCTGAGTCCGGAAGAAAACCTGATAATGTCGGCCCACTACTTTGAAGGGCTGCGCATTCAGCTGGAACTGGGCAGAGCCA
TGGCGCTTTTTGCCGGTAAAAACCCGCATGCGCAAAGTATGGTTGTGGGCGGCATGACCTGCTACGACAGCCTGCGTCCG
GAAGTTATCGGACACTTCCGCAAGATCTGGGAACACTGCAAGGAGTTTGTTGAAAACGCCATGATGCCCGACATCCTGCT
GATGACCAGACGCTACCCTGAAGCGCTGGCCTACGGCAGAACATCCAACTTCTTTGCCTTTGCAGATTTTCATGATCCGG
TAACAGGCAAAGACCCGTACTTCACTTCGGGCGTGCTGTGGGGCAACGATCTTACAAAGCACGAAGAGCTGGACCCCGGC
CATATCAACGAACATGTGGCGCGCAGCTGGTACGAAGGCGATACCCCGCGCACGCCCTATGAAGGCGCTACAGAGCCGCA
CTACACATCATATGAGGACAAGGAGAAGTATTCCTGGTCCAAGGCTCCGCGCTACAAAAATGAAGCAATGGAGACCGGCC
CGCTGGCACGGCGCGCGCTGGCCTACGCCCGTAAAGAGCAGGAAACAAGAACCATGCTGGATACTTTTTTCAAAGATTCC
GGCATGAAGCCTGATCAGTTGTTTTCCACCATGGGGCGTACCGTATGCCGTGTGGTGGAAACAACCATGCTTATGCGGCG
CATGCAGGGCTGGATTGACGATACCGAAGCACGCATCAAAGCCGGTGAAGACACCATATACACAGAATGGAAAATGCCGG
ACTCCGCCCGCGGCGTGGGCTTTTGCTGTGTAACCCGCGGCGCCCTGTCGCACTGGATAGACATCAAGGAAGCTAAAATA
CACAACTTCCAGATGGTCGTACCTTCTACATGGAATCTGGGGCCCCGCTGCGCCAACGGAAAACTGAGCGCGGGCGAGCA
GTCACTTATCGGATGCCCGTGTCCCGACCCGGACCGCCCTGTGGAAATTCTGCGCACCATTCATTCATTCGATCCCTGCA
TCGCCTGCTCGGTTCATCTGCTGGATGCACAGAAAAAGCCTCTCGGCAGCTTTAAGGTATTGTAG

Upstream 100 bases:

>100_bases
CAGCGAACCCAACTTCTGGGACAAATACTCGCCCTTTTTCCACCCCATTGAAGACGGCCCCGAAAGCTAACCCGCGCCGC
CCGAAAGGAGATAAGACCGA

Downstream 100 bases:

>100_bases
CCACACACAAGCTCACTCTGTCTTGTCCCGCGGCCCTGACCTTACAGTCGGGGCCGCGTTTTTTTACGGACAATACAGAC
CGTAGAAAAAGCACGCCACC

Product: periplasmic (NiFe) hydrogenase large subunit, isozyme 2

Products: NA

Alternate protein names: NiFe hydrogenlyase large chain [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSQKKMNHTGKRLIVDPVTRIEGHLKIEVELENNKVKDAWVSTQLFRGIEMILKGRPPEDAPLFTQRACGVCTNTHALTS
IRAIEDALEIKVPPLAQLMRHLILSALIVHDHLVHFYHLHGLDWIDVAAATTADPAKAGKIVGSTSNRSDDPSELFIIQK
RLKEFVKSGQLGFLEKAYFLGGNDAYKLSPEENLIMSAHYFEGLRIQLELGRAMALFAGKNPHAQSMVVGGMTCYDSLRP
EVIGHFRKIWEHCKEFVENAMMPDILLMTRRYPEALAYGRTSNFFAFADFHDPVTGKDPYFTSGVLWGNDLTKHEELDPG
HINEHVARSWYEGDTPRTPYEGATEPHYTSYEDKEKYSWSKAPRYKNEAMETGPLARRALAYARKEQETRTMLDTFFKDS
GMKPDQLFSTMGRTVCRVVETTMLMRRMQGWIDDTEARIKAGEDTIYTEWKMPDSARGVGFCCVTRGALSHWIDIKEAKI
HNFQMVVPSTWNLGPRCANGKLSAGEQSLIGCPCPDPDRPVEILRTIHSFDPCIACSVHLLDAQKKPLGSFKVL

Sequences:

>Translated_554_residues
MSQKKMNHTGKRLIVDPVTRIEGHLKIEVELENNKVKDAWVSTQLFRGIEMILKGRPPEDAPLFTQRACGVCTNTHALTS
IRAIEDALEIKVPPLAQLMRHLILSALIVHDHLVHFYHLHGLDWIDVAAATTADPAKAGKIVGSTSNRSDDPSELFIIQK
RLKEFVKSGQLGFLEKAYFLGGNDAYKLSPEENLIMSAHYFEGLRIQLELGRAMALFAGKNPHAQSMVVGGMTCYDSLRP
EVIGHFRKIWEHCKEFVENAMMPDILLMTRRYPEALAYGRTSNFFAFADFHDPVTGKDPYFTSGVLWGNDLTKHEELDPG
HINEHVARSWYEGDTPRTPYEGATEPHYTSYEDKEKYSWSKAPRYKNEAMETGPLARRALAYARKEQETRTMLDTFFKDS
GMKPDQLFSTMGRTVCRVVETTMLMRRMQGWIDDTEARIKAGEDTIYTEWKMPDSARGVGFCCVTRGALSHWIDIKEAKI
HNFQMVVPSTWNLGPRCANGKLSAGEQSLIGCPCPDPDRPVEILRTIHSFDPCIACSVHLLDAQKKPLGSFKVL
>Mature_553_residues
SQKKMNHTGKRLIVDPVTRIEGHLKIEVELENNKVKDAWVSTQLFRGIEMILKGRPPEDAPLFTQRACGVCTNTHALTSI
RAIEDALEIKVPPLAQLMRHLILSALIVHDHLVHFYHLHGLDWIDVAAATTADPAKAGKIVGSTSNRSDDPSELFIIQKR
LKEFVKSGQLGFLEKAYFLGGNDAYKLSPEENLIMSAHYFEGLRIQLELGRAMALFAGKNPHAQSMVVGGMTCYDSLRPE
VIGHFRKIWEHCKEFVENAMMPDILLMTRRYPEALAYGRTSNFFAFADFHDPVTGKDPYFTSGVLWGNDLTKHEELDPGH
INEHVARSWYEGDTPRTPYEGATEPHYTSYEDKEKYSWSKAPRYKNEAMETGPLARRALAYARKEQETRTMLDTFFKDSG
MKPDQLFSTMGRTVCRVVETTMLMRRMQGWIDDTEARIKAGEDTIYTEWKMPDSARGVGFCCVTRGALSHWIDIKEAKIH
NFQMVVPSTWNLGPRCANGKLSAGEQSLIGCPCPDPDRPVEILRTIHSFDPCIACSVHLLDAQKKPLGSFKVL

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=568, Percent_Identity=39.612676056338, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1787207, Length=588, Percent_Identity=38.265306122449, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 62633; Mature: 62501

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKKMNHTGKRLIVDPVTRIEGHLKIEVELENNKVKDAWVSTQLFRGIEMILKGRPPED
CCCCCCCCCCCEEEECCHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
APLFTQRACGVCTNTHALTSIRAIEDALEIKVPPLAQLMRHLILSALIVHDHLVHFYHLH
CCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLDWIDVAAATTADPAKAGKIVGSTSNRSDDPSELFIIQKRLKEFVKSGQLGFLEKAYFL
CCCHHHHHHCCCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHEEEE
GGNDAYKLSPEENLIMSAHYFEGLRIQLELGRAMALFAGKNPHAQSMVVGGMTCYDSLRP
CCCCCEEECCCCCEEEEEEECCCEEEEEEECCEEEEEECCCCCCCCEEECCHHHHHHCCH
EVIGHFRKIWEHCKEFVENAMMPDILLMTRRYPEALAYGRTSNFFAFADFHDPVTGKDPY
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHCCCCCCEEEEECCCCCCCCCCCC
FTSGVLWGNDLTKHEELDPGHINEHVARSWYEGDTPRTPYEGATEPHYTSYEDKEKYSWS
EECCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCC
KAPRYKNEAMETGPLARRALAYARKEQETRTMLDTFFKDSGMKPDQLFSTMGRTVCRVVE
CCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TTMLMRRMQGWIDDTEARIKAGEDTIYTEWKMPDSARGVGFCCVTRGALSHWIDIKEAKI
HHHHHHHHHCCCCCCHHHEECCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHCHHHCCC
HNFQMVVPSTWNLGPRCANGKLSAGEQSLIGCPCPDPDRPVEILRTIHSFDPCIACSVHL
CEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCHHHHEEEE
LDAQKKPLGSFKVL
ECCCCCCCCCCCCC
>Mature Secondary Structure 
SQKKMNHTGKRLIVDPVTRIEGHLKIEVELENNKVKDAWVSTQLFRGIEMILKGRPPED
CCCCCCCCCCEEEECCHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
APLFTQRACGVCTNTHALTSIRAIEDALEIKVPPLAQLMRHLILSALIVHDHLVHFYHLH
CCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLDWIDVAAATTADPAKAGKIVGSTSNRSDDPSELFIIQKRLKEFVKSGQLGFLEKAYFL
CCCHHHHHHCCCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHEEEE
GGNDAYKLSPEENLIMSAHYFEGLRIQLELGRAMALFAGKNPHAQSMVVGGMTCYDSLRP
CCCCCEEECCCCCEEEEEEECCCEEEEEEECCEEEEEECCCCCCCCEEECCHHHHHHCCH
EVIGHFRKIWEHCKEFVENAMMPDILLMTRRYPEALAYGRTSNFFAFADFHDPVTGKDPY
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHCCCCCCEEEEECCCCCCCCCCCC
FTSGVLWGNDLTKHEELDPGHINEHVARSWYEGDTPRTPYEGATEPHYTSYEDKEKYSWS
EECCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCC
KAPRYKNEAMETGPLARRALAYARKEQETRTMLDTFFKDSGMKPDQLFSTMGRTVCRVVE
CCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TTMLMRRMQGWIDDTEARIKAGEDTIYTEWKMPDSARGVGFCCVTRGALSHWIDIKEAKI
HHHHHHHHHCCCCCCHHHEECCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHCHHHCCC
HNFQMVVPSTWNLGPRCANGKLSAGEQSLIGCPCPDPDRPVEILRTIHSFDPCIACSVHL
CEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCHHHHEEEE
LDAQKKPLGSFKVL
ECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3322743; 2651421; 3322275; 7854413 [H]