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The map label for this gene is purN [H]

Identifier: 78357876

GI number: 78357876

Start: 2822067

End: 2822741

Strand: Reverse

Name: purN [H]

Synonym: Dde_2835

Alternate gene names: 78357876

Gene position: 2822741-2822067 (Counterclockwise)

Preceding gene: 78357877

Following gene: 78357875

Centisome position: 75.67

GC content: 63.26

Gene sequence:

>675_bases
ATGCCGCTGCAACTGGCGGTGCTGGCATCGGGCAACGGGTCAAACCTGCAGGCCGTGCTGGACCGCGCTGCACAGGGTGT
GCTGGATGTAGAGGTGCGCCTTGTGGCGTCCAACAAGGAAGACGCCTGCGCCCTTGATCGGGCGCGGCGTGCAGGCATTC
CCGTGTGGGCGCGCAATCACGGCAGTTTTGCCGGCAGAGAGGAATTTGACGCGGCGCTGGTGGACGCCATACGGGCCAGC
GGGGCCGACACCATCATGCTGGCGGGATACATGCGTCTGCTTACACCGTATTTTCTCAATGCCTTTCCGGGCAGGGTGCT
CAATGTGCATCCGGCCCTGCTGCCCAGCTTTCCCGGAGTAAGGGGAGTAGCCGACGCCGTGGAGTACGGTGTGCGTGTGG
CAGGGTGCACAGTGCATTTTGTTGATGAAATAATGGACCACGGACCGGTGATCATTCAGGCGGCTGTTCCCGTGTCTGCC
TGCGATTCCCGCGATGACGTGCTGCAGCGGGTGCATGCGGCAGAGCACCGCATTTATCCTCAGGCATTGCAGTGGCTGGC
CGAAGGCCGGCTGTCGCTGCAGGGCCGGGTGGTGCATCTGGCACCGTCACGGCGTCCTGCGGCCGGTGCAGCGGCGGATA
TGCTGGTTTCGCCCGCGCTGGAACAAGGTTTTTAA

Upstream 100 bases:

>100_bases
GGTGATCCCGTGCATGTGTTGTGATACGAGATGATGTTGTTACGGCTGCCCGTCATGTGCGGCGTGCAGTGTCTTTGATT
ATAGCAGGACAGGAGGCATT

Downstream 100 bases:

>100_bases
CTCTTCCGGGCAGAGTGGCTGCCGCGGTGTGCCGTGGCGGCATGTGCACCCGTGCGGCCGCTGCGGCCGAGGTCCGGTGC
CGTTTGCAGCGGTGCATGGT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPLQLAVLASGNGSNLQAVLDRAAQGVLDVEVRLVASNKEDACALDRARRAGIPVWARNHGSFAGREEFDAALVDAIRAS
GADTIMLAGYMRLLTPYFLNAFPGRVLNVHPALLPSFPGVRGVADAVEYGVRVAGCTVHFVDEIMDHGPVIIQAAVPVSA
CDSRDDVLQRVHAAEHRIYPQALQWLAEGRLSLQGRVVHLAPSRRPAAGAAADMLVSPALEQGF

Sequences:

>Translated_224_residues
MPLQLAVLASGNGSNLQAVLDRAAQGVLDVEVRLVASNKEDACALDRARRAGIPVWARNHGSFAGREEFDAALVDAIRAS
GADTIMLAGYMRLLTPYFLNAFPGRVLNVHPALLPSFPGVRGVADAVEYGVRVAGCTVHFVDEIMDHGPVIIQAAVPVSA
CDSRDDVLQRVHAAEHRIYPQALQWLAEGRLSLQGRVVHLAPSRRPAAGAAADMLVSPALEQGF
>Mature_223_residues
PLQLAVLASGNGSNLQAVLDRAAQGVLDVEVRLVASNKEDACALDRARRAGIPVWARNHGSFAGREEFDAALVDAIRASG
ADTIMLAGYMRLLTPYFLNAFPGRVLNVHPALLPSFPGVRGVADAVEYGVRVAGCTVHFVDEIMDHGPVIIQAAVPVSAC
DSRDDVLQRVHAAEHRIYPQALQWLAEGRLSLQGRVVHLAPSRRPAAGAAADMLVSPALEQGF

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=44.7368421052632, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=44.7368421052632, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=44.7368421052632, Blast_Score=163, Evalue=1e-40,
Organism=Escherichia coli, GI1788846, Length=211, Percent_Identity=45.0236966824645, Blast_Score=186, Evalue=9e-49,
Organism=Escherichia coli, GI1787483, Length=192, Percent_Identity=32.2916666666667, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=40.3314917127072, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=30.6532663316583, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=47.0899470899471, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23873; Mature: 23741

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLQLAVLASGNGSNLQAVLDRAAQGVLDVEVRLVASNKEDACALDRARRAGIPVWARNH
CCEEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHCCCCEEECCC
GSFAGREEFDAALVDAIRASGADTIMLAGYMRLLTPYFLNAFPGRVLNVHPALLPSFPGV
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHCCCCCCH
RGVADAVEYGVRVAGCTVHFVDEIMDHGPVIIQAAVPVSACDSRDDVLQRVHAAEHRIYP
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHCCCH
QALQWLAEGRLSLQGRVVHLAPSRRPAAGAAADMLVSPALEQGF
HHHHHHHCCCCEECCEEEEECCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLQLAVLASGNGSNLQAVLDRAAQGVLDVEVRLVASNKEDACALDRARRAGIPVWARNH
CEEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHCCCCEEECCC
GSFAGREEFDAALVDAIRASGADTIMLAGYMRLLTPYFLNAFPGRVLNVHPALLPSFPGV
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHCCCCCCH
RGVADAVEYGVRVAGCTVHFVDEIMDHGPVIIQAAVPVSACDSRDDVLQRVHAAEHRIYP
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHCCCH
QALQWLAEGRLSLQGRVVHLAPSRRPAAGAAADMLVSPALEQGF
HHHHHHHCCCCEECCEEEEECCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]