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The map label for this gene is hsdM [H]

Identifier: 78357541

GI number: 78357541

Start: 2521642

End: 2523108

Strand: Reverse

Name: hsdM [H]

Synonym: Dde_2498

Alternate gene names: 78357541

Gene position: 2523108-2521642 (Counterclockwise)

Preceding gene: 78357542

Following gene: 78357540

Centisome position: 67.64

GC content: 60.05

Gene sequence:

>1467_bases
ATGTCACTCACCACCCTGATCAAAGCCATTCAGGACATCATGCGCAAGGATGTCGGCGTCGATGGCGACGCTCAGCGCAT
CAGCCAGATGGTCTGGCTGATTTTCCTGAAAATCTTCGATGACAAGGAACAGGAGTGGCAGCTCACCGTGCCCGGCTACA
AATCGCCGCTGCCCAGCCGTTTCCGCTGGTCCAGCTGGGCCAAGAACCCCGAGGGGATGACCGGTGAGGAGCTGATCGAC
TTTGTCAACAACGACCTCTTCCCGGCGCTCAAGAAACTGGCCACCGCCGCCGGTGTGTCGCCCCATGGCAAGGTGGTCGG
TTCGGTCTTCGAGGACGCCTACAACTACATGAAGTCCGGCACCCTGCTGCGCCAGGTGATCAACACCATCGAAGAAGATG
TGGACTTCAACAAATCCGGCGACCGTCACCTGTTCAACGACATCTACGAAAAGATCCTCTCCGACCTGCAATCCGCCGGG
AACGCGGGCGAGTACTACACCCCCCGCGCCGTCACCCAGTTCATGGTCGACATGCTCGACCCACAGCTTGGGCAAACCAT
TCTCGACCCGGCCTGCGGCACCGGCGGCTTTCTGACCTGCGCCATCGAGCACCTGAACAAACAGGTCAAAACCGCCGAGG
ATCGCAAGCGCCTGCAGGAGTGTATTCACGGCGTGGAGAAGAAACCGCTGCCGCACATGCTGGCCATGACCAACATGATG
CTGCACGGCATCGACGTGCCCACCAACGTCCGCCACGACAATACCCTGAGCCGCCCGCTGAAGGATTACGGCCCCCGCGA
CCGGGTGGACCTGATCATCACCAATCCGCCCTTCGGCGGTATGGAAGAAGACGGCATCGAGAACAACTTTCCGCGTAAGT
ACCAGACCCGCGAGACCGCCGACCTCTTCATGGCGCTGATCATGCACCTTTTAAAACACGACACCGGCAAGGCGGCAGTA
GTCCTGCCCGACGGTTTTCTCTTTGGTGAGGGCGTCAAAACGACGATCAAGCGCGAACTGCTGGAGGAGTTCAACCTGCA
CACCATCGTGCGCCTGCCAAAAGGCGTGTTCAGCCCCTATACCAGCATCGCCACCAACATCCTCTTTTTTGAGAAGGGCG
GGCCTACGAAAGAGGTCTGGTTCTTCGAACACCCGTATCCCGAGGGCTACAAGTCCTACTCCCGCTCCAAGCCCCTGACC
ATCGCCGAGTTCGATCTCGAAAAGGCCTGGTGGGGCGGGGCCTCCCGCCGTGGGCGCAAGACCACCGAGCAGACCTGGAA
GGTCTCGGCCAAGGAGCTCGCCGAGCGCAACTACAACCTGGACTGCAAGAACCCCCACGAGGTGGAGGTCAACCACCGCG
ATCCGGAAGAGCTGATGGCCGAATACCAGCAGATCGTCCACCAACTCGAAGCGGCGCAGGCCGCCTTGAAAGCCGAGTTG
ATGGCCTGTCTGGGGGGAAATGCGTAA

Upstream 100 bases:

>100_bases
TCCGCGCCTTTGGTGGGCTGGATCAGTATCAGCAGGCTGTCAACGAACTGGAACAGGCCCTCTATAGCGCCTGACAAAAA
GAACTGGAGATAAAACAACC

Downstream 100 bases:

>100_bases
TGAAAGAAGTCTCACACGAAGGCACGAAGACACGAAGAAAAGAGTGGTACTCACGCGAAGACGCGAAGACGCGAAGGGAT
GAAGAGAACAGCCGTCATAC

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSLTTLIKAIQDIMRKDVGVDGDAQRISQMVWLIFLKIFDDKEQEWQLTVPGYKSPLPSRFRWSSWAKNPEGMTGEELID
FVNNDLFPALKKLATAAGVSPHGKVVGSVFEDAYNYMKSGTLLRQVINTIEEDVDFNKSGDRHLFNDIYEKILSDLQSAG
NAGEYYTPRAVTQFMVDMLDPQLGQTILDPACGTGGFLTCAIEHLNKQVKTAEDRKRLQECIHGVEKKPLPHMLAMTNMM
LHGIDVPTNVRHDNTLSRPLKDYGPRDRVDLIITNPPFGGMEEDGIENNFPRKYQTRETADLFMALIMHLLKHDTGKAAV
VLPDGFLFGEGVKTTIKRELLEEFNLHTIVRLPKGVFSPYTSIATNILFFEKGGPTKEVWFFEHPYPEGYKSYSRSKPLT
IAEFDLEKAWWGGASRRGRKTTEQTWKVSAKELAERNYNLDCKNPHEVEVNHRDPEELMAEYQQIVHQLEAAQAALKAEL
MACLGGNA

Sequences:

>Translated_488_residues
MSLTTLIKAIQDIMRKDVGVDGDAQRISQMVWLIFLKIFDDKEQEWQLTVPGYKSPLPSRFRWSSWAKNPEGMTGEELID
FVNNDLFPALKKLATAAGVSPHGKVVGSVFEDAYNYMKSGTLLRQVINTIEEDVDFNKSGDRHLFNDIYEKILSDLQSAG
NAGEYYTPRAVTQFMVDMLDPQLGQTILDPACGTGGFLTCAIEHLNKQVKTAEDRKRLQECIHGVEKKPLPHMLAMTNMM
LHGIDVPTNVRHDNTLSRPLKDYGPRDRVDLIITNPPFGGMEEDGIENNFPRKYQTRETADLFMALIMHLLKHDTGKAAV
VLPDGFLFGEGVKTTIKRELLEEFNLHTIVRLPKGVFSPYTSIATNILFFEKGGPTKEVWFFEHPYPEGYKSYSRSKPLT
IAEFDLEKAWWGGASRRGRKTTEQTWKVSAKELAERNYNLDCKNPHEVEVNHRDPEELMAEYQQIVHQLEAAQAALKAEL
MACLGGNA
>Mature_487_residues
SLTTLIKAIQDIMRKDVGVDGDAQRISQMVWLIFLKIFDDKEQEWQLTVPGYKSPLPSRFRWSSWAKNPEGMTGEELIDF
VNNDLFPALKKLATAAGVSPHGKVVGSVFEDAYNYMKSGTLLRQVINTIEEDVDFNKSGDRHLFNDIYEKILSDLQSAGN
AGEYYTPRAVTQFMVDMLDPQLGQTILDPACGTGGFLTCAIEHLNKQVKTAEDRKRLQECIHGVEKKPLPHMLAMTNMML
HGIDVPTNVRHDNTLSRPLKDYGPRDRVDLIITNPPFGGMEEDGIENNFPRKYQTRETADLFMALIMHLLKHDTGKAAVV
LPDGFLFGEGVKTTIKRELLEEFNLHTIVRLPKGVFSPYTSIATNILFFEKGGPTKEVWFFEHPYPEGYKSYSRSKPLTI
AEFDLEKAWWGGASRRGRKTTEQTWKVSAKELAERNYNLDCKNPHEVEVNHRDPEELMAEYQQIVHQLEAAQAALKAELM
ACLGGNA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=442, Percent_Identity=27.1493212669683, Blast_Score=138, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55257; Mature: 55126

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTTLIKAIQDIMRKDVGVDGDAQRISQMVWLIFLKIFDDKEQEWQLTVPGYKSPLPSR
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
FRWSSWAKNPEGMTGEELIDFVNNDLFPALKKLATAAGVSPHGKVVGSVFEDAYNYMKSG
CCCHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TLLRQVINTIEEDVDFNKSGDRHLFNDIYEKILSDLQSAGNAGEYYTPRAVTQFMVDMLD
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
PQLGQTILDPACGTGGFLTCAIEHLNKQVKTAEDRKRLQECIHGVEKKPLPHMLAMTNMM
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LHGIDVPTNVRHDNTLSRPLKDYGPRDRVDLIITNPPFGGMEEDGIENNFPRKYQTRETA
HHCCCCCCCCCCCCHHHCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
DLFMALIMHLLKHDTGKAAVVLPDGFLFGEGVKTTIKRELLEEFNLHTIVRLPKGVFSPY
HHHHHHHHHHHHCCCCCEEEEECCCCEECCCHHHHHHHHHHHHHCCHHHHHCCHHHHCHH
TSIATNILFFEKGGPTKEVWFFEHPYPEGYKSYSRSKPLTIAEFDLEKAWWGGASRRGRK
HHHHHHEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCC
TTEQTWKVSAKELAERNYNLDCKNPHEVEVNHRDPEELMAEYQQIVHQLEAAQAALKAEL
CHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MACLGGNA
HHHHCCCC
>Mature Secondary Structure 
SLTTLIKAIQDIMRKDVGVDGDAQRISQMVWLIFLKIFDDKEQEWQLTVPGYKSPLPSR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
FRWSSWAKNPEGMTGEELIDFVNNDLFPALKKLATAAGVSPHGKVVGSVFEDAYNYMKSG
CCCHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TLLRQVINTIEEDVDFNKSGDRHLFNDIYEKILSDLQSAGNAGEYYTPRAVTQFMVDMLD
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
PQLGQTILDPACGTGGFLTCAIEHLNKQVKTAEDRKRLQECIHGVEKKPLPHMLAMTNMM
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LHGIDVPTNVRHDNTLSRPLKDYGPRDRVDLIITNPPFGGMEEDGIENNFPRKYQTRETA
HHCCCCCCCCCCCCHHHCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
DLFMALIMHLLKHDTGKAAVVLPDGFLFGEGVKTTIKRELLEEFNLHTIVRLPKGVFSPY
HHHHHHHHHHHHCCCCCEEEEECCCCEECCCHHHHHHHHHHHHHCCHHHHHCCHHHHCHH
TSIATNILFFEKGGPTKEVWFFEHPYPEGYKSYSRSKPLTIAEFDLEKAWWGGASRRGRK
HHHHHHEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCC
TTEQTWKVSAKELAERNYNLDCKNPHEVEVNHRDPEELMAEYQQIVHQLEAAQAALKAEL
CHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MACLGGNA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]