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The map label for this gene is hybC [C]

Identifier: 78357178

GI number: 78357178

Start: 2182177

End: 2183643

Strand: Direct

Name: hybC [C]

Synonym: Dde_2135

Alternate gene names: 78357178

Gene position: 2182177-2183643 (Clockwise)

Preceding gene: 78357177

Following gene: 78357179

Centisome position: 58.5

GC content: 57.6

Gene sequence:

>1467_bases
ATGTCTGGTTGCACGAATAAAATGGCAGCCGGTGGCGTGAGTGGCAAAACTAAAATTGCCATCGACCCGGTTACCAGAAT
CGAAGGCCACCTGAAGGCCGAAGTCGTCGTGGAAGGGGGCAAAGTCGTTGACGCCCACATTTCCGGCGGCATGTTCCGAG
GCTTTGAGAACATTCTGCGCGGGCGCGATCCGCGCGATGCGTCTCAGATTGTACAGCGTATCTGCGGCGTATGCCCCACT
GCACACTCGACCGCTTCGGTCATGGCTCTGGATAAGGCTTTCGGTGTAAAGGTGACCACAAACGGCCGCCTGACCAGAAA
CCTGATTCTGGGTGCCAACTACATGCAGTCGCATATTCTGCATTTCTATCATCTGGCCGCACTGGACTTTGTGCAGGGGC
CGGAAACCGCACCTTTTGTCCCCCGCTTCAAAAATCCCGACCTGCGCCTGCCTTCCGCTGTGAATCAGGTGGCTGTCGAC
CAGTATCTTGAAGCACTTGAAGTCCGCCGCATCTGCCACGAAATGGTGGCCATTTTCGGCGGCAGAATGCCTCACGTACA
GGGTCAGGTTGTAGGCGGCACCACAGAAATTCCCACCAAGGAAGCTCTTGTCGAATACGCAGCCCGCTTCAAAAAAGTAC
GCAAATTCGTGGAAGAAAAATACGTTCCCGTTGTGTACCTTGTGGGCAGCGTCTACAAAGACCTTTTCGCTTTCGGTCAG
GGCTACAAGAACTGCGTCGCTTTCGGCGTGTTCCCGCTCAATGACGAAGGCACCGAATTCGCCCTGAAGCCCGGCGTATA
CATGGATGGCGAGGACAAGCCCTTTGATGCCCGCCTCATCAAGGAATACGTCAAATACAGCTGGTTCGACGACAGCACAA
CCGGCCTGCACTACACTGAAGGCCAGACCATACCTGACCCCGAAAAGCCCGGCGCATACAGCTTCGTCAAGGCTCCCCGC
TACAACGGCAAGCCCTGTGAAGTGGGCCCGCTGGCCCGTATGTGGGTGGCCAACCCCGAACTGAGCCCCATGGGCCAGAA
GATGCTCAAGGAACACTACGGCATCGACGCCAAGCGCTTCCGCGATATCGGTGCCGAAGCCGCCTTTTCGCTGATGGGCC
GCCACGTGGCCCGCGCAGAAGAAACCTGGCTGACCACCAACTACATAGAGCGCTGGCTCAAGGAAGTGGTGCCGGGAGCT
GAAACCTACGTGCCTTCCGAAATTCCCGAACAGGCCGAAGGAACGGGCTTCACCGAAGCTCCCCGCGGTTCGCTGCTGCA
CTACATCGACATCAAGGATTCGGTGATCAGCAACTACCAGATAGTCTCTGCCACACTGTGGAACTGCAACCCCCGTGACG
ACATGGGCAACCGCGGTCCCGTGGAAGAAGCTCTCATCGGCACCCCCGTGCCGGATCTGGAAAACCCGGTGAACATCGCG
CGGCTCATACGCGCGTTTGACCCGTGA

Upstream 100 bases:

>100_bases
ACGCTGTCTGCATCGGCTGTGTGGAACCCGACTTCCCCGACGGCAAGTCCCCGTTCTACGAGTCTTAACAAGCGCTTGAC
CAACATCAGGAGGAGACCAC

Downstream 100 bases:

>100_bases
CTGGGCTGCGCCGTGCACGTGCTGCACGCAGAGTCCGGTAAGGTTTCCGTAGTCGAATTCTAGTCGGATACGCACCTTGC
TTCGTAAGGGGCCTTCGGGC

Product: periplasmic (NiFeSe) hydrogenase large subunit, selenocysteine-containing

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSGCTNKMAAGGVSGKTKIAIDPVTRIEGHLKAEVVVEGGKVVDAHISGGMFRGFENILRGRDPRDASQIVQRICGVCPT
AHSTASVMALDKAFGVKVTTNGRLTRNLILGANYMQSHILHFYHLAALDFVQGPETAPFVPRFKNPDLRLPSAVNQVAVD
QYLEALEVRRICHEMVAIFGGRMPHVQGQVVGGTTEIPTKEALVEYAARFKKVRKFVEEKYVPVVYLVGSVYKDLFAFGQ
GYKNCVAFGVFPLNDEGTEFALKPGVYMDGEDKPFDARLIKEYVKYSWFDDSTTGLHYTEGQTIPDPEKPGAYSFVKAPR
YNGKPCEVGPLARMWVANPELSPMGQKMLKEHYGIDAKRFRDIGAEAAFSLMGRHVARAEETWLTTNYIERWLKEVVPGA
ETYVPSEIPEQAEGTGFTEAPRGSLLHYIDIKDSVISNYQIVSATLWNCNPRDDMGNRGPVEEALIGTPVPDLENPVNIA
RLIRAFDP

Sequences:

>Translated_488_residues
MSGCTNKMAAGGVSGKTKIAIDPVTRIEGHLKAEVVVEGGKVVDAHISGGMFRGFENILRGRDPRDASQIVQRICGVCPT
AHSTASVMALDKAFGVKVTTNGRLTRNLILGANYMQSHILHFYHLAALDFVQGPETAPFVPRFKNPDLRLPSAVNQVAVD
QYLEALEVRRICHEMVAIFGGRMPHVQGQVVGGTTEIPTKEALVEYAARFKKVRKFVEEKYVPVVYLVGSVYKDLFAFGQ
GYKNCVAFGVFPLNDEGTEFALKPGVYMDGEDKPFDARLIKEYVKYSWFDDSTTGLHYTEGQTIPDPEKPGAYSFVKAPR
YNGKPCEVGPLARMWVANPELSPMGQKMLKEHYGIDAKRFRDIGAEAAFSLMGRHVARAEETWLTTNYIERWLKEVVPGA
ETYVPSEIPEQAEGTGFTEAPRGSLLHYIDIKDSVISNYQIVSATLWNCNPRDDMGNRGPVEEALIGTPVPDLENPVNIA
RLIRAFDP
>Mature_487_residues
SGCTNKMAAGGVSGKTKIAIDPVTRIEGHLKAEVVVEGGKVVDAHISGGMFRGFENILRGRDPRDASQIVQRICGVCPTA
HSTASVMALDKAFGVKVTTNGRLTRNLILGANYMQSHILHFYHLAALDFVQGPETAPFVPRFKNPDLRLPSAVNQVAVDQ
YLEALEVRRICHEMVAIFGGRMPHVQGQVVGGTTEIPTKEALVEYAARFKKVRKFVEEKYVPVVYLVGSVYKDLFAFGQG
YKNCVAFGVFPLNDEGTEFALKPGVYMDGEDKPFDARLIKEYVKYSWFDDSTTGLHYTEGQTIPDPEKPGAYSFVKAPRY
NGKPCEVGPLARMWVANPELSPMGQKMLKEHYGIDAKRFRDIGAEAAFSLMGRHVARAEETWLTTNYIERWLKEVVPGAE
TYVPSEIPEQAEGTGFTEAPRGSLLHYIDIKDSVISNYQIVSATLWNCNPRDDMGNRGPVEEALIGTPVPDLENPVNIAR
LIRAFDP

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=544, Percent_Identity=34.9264705882353, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1787207, Length=565, Percent_Identity=30.2654867256637, Blast_Score=236, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 53991; Mature: 53860

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00086 CYTOCHROME_P450 ; PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGCTNKMAAGGVSGKTKIAIDPVTRIEGHLKAEVVVEGGKVVDAHISGGMFRGFENILR
CCCCCCCHHCCCCCCCCEEEECCHHHHCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHC
GRDPRDASQIVQRICGVCPTAHSTASVMALDKAFGVKVTTNGRLTRNLILGANYMQSHIL
CCCCCCHHHHHHHHHCCCCCCCCCHHHHEEHHHCCEEEECCCCEEEHHEECCHHHHHHHH
HFYHLAALDFVQGPETAPFVPRFKNPDLRLPSAVNQVAVDQYLEALEVRRICHEMVAIFG
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRMPHVQGQVVGGTTEIPTKEALVEYAARFKKVRKFVEEKYVPVVYLVGSVYKDLFAFGQ
CCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
GYKNCVAFGVFPLNDEGTEFALKPGVYMDGEDKPFDARLIKEYVKYSWFDDSTTGLHYTE
CHHHEEEEEEEECCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECC
GQTIPDPEKPGAYSFVKAPRYNGKPCEVGPLARMWVANPELSPMGQKMLKEHYGIDAKRF
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHCCCHHHH
RDIGAEAAFSLMGRHVARAEETWLTTNYIERWLKEVVPGAETYVPSEIPEQAEGTGFTEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCC
PRGSLLHYIDIKDSVISNYQIVSATLWNCNPRDDMGNRGPVEEALIGTPVPDLENPVNIA
CCCCEEEEEECCHHHHCCEEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHH
RLIRAFDP
HHHHHCCC
>Mature Secondary Structure 
SGCTNKMAAGGVSGKTKIAIDPVTRIEGHLKAEVVVEGGKVVDAHISGGMFRGFENILR
CCCCCCHHCCCCCCCCEEEECCHHHHCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHC
GRDPRDASQIVQRICGVCPTAHSTASVMALDKAFGVKVTTNGRLTRNLILGANYMQSHIL
CCCCCCHHHHHHHHHCCCCCCCCCHHHHEEHHHCCEEEECCCCEEEHHEECCHHHHHHHH
HFYHLAALDFVQGPETAPFVPRFKNPDLRLPSAVNQVAVDQYLEALEVRRICHEMVAIFG
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRMPHVQGQVVGGTTEIPTKEALVEYAARFKKVRKFVEEKYVPVVYLVGSVYKDLFAFGQ
CCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
GYKNCVAFGVFPLNDEGTEFALKPGVYMDGEDKPFDARLIKEYVKYSWFDDSTTGLHYTE
CHHHEEEEEEEECCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECC
GQTIPDPEKPGAYSFVKAPRYNGKPCEVGPLARMWVANPELSPMGQKMLKEHYGIDAKRF
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHCCCHHHH
RDIGAEAAFSLMGRHVARAEETWLTTNYIERWLKEVVPGAETYVPSEIPEQAEGTGFTEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCC
PRGSLLHYIDIKDSVISNYQIVSATLWNCNPRDDMGNRGPVEEALIGTPVPDLENPVNIA
CCCCEEEEEECCHHHHCCEEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHH
RLIRAFDP
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]