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The map label for this gene is hyaA [C]

Identifier: 78357177

GI number: 78357177

Start: 2181260

End: 2182144

Strand: Direct

Name: hyaA [C]

Synonym: Dde_2134

Alternate gene names: 78357177

Gene position: 2181260-2182144 (Clockwise)

Preceding gene: 78357173

Following gene: 78357178

Centisome position: 58.48

GC content: 56.61

Gene sequence:

>885_bases
ATGTTCCATCCCGCAATTCGCGACGCACTGGCTGATACGCTGAAAGGCGACCGTCCGCCCGTGCTCTGGCTGCAGGGTCA
GGGCTGTACCGGGTGCTCGGTCTCGCTGCTCAACAGCGTCCACCCCAGCATCAAGGACGTTCTTCTTGATGTGATCAGTC
TTGAGTTCCACCCCACGGTCATGGCGTGGGAAGGTGAACACGCCATCGAACACATGATGAAGATCGCAGAGAAGTACGAA
GGCAAGTTCTTTGTGGTTGTTGAAGGTTCCATTCCGCTTGCTGCGGACGGCAAATACTGCATCATCGGCGATATAGACCA
CAAAGAAATCTCCATCGCCGACGCCATGCGCGACATCGCCGCAAAATCCGCTGCTGTTCTTGCAGTGGGCACCTGTGCAG
CCTACGGCGGCATTCCCGCGGCCGAAGGCAACCTGACCGAAGCAGTTTCCGTTCAGCAGTTCTTTGAGCAGGAAGGCATA
AAAACTCCTGTTGTCAATATTCCCGGCTGTCCGCCCCACCCCGACTGGATTGTGGGTACGGTCGTAGTCGCGCTTGACGC
CATCAAAAAGCACGGCCTGCAGGGCGGTCTGGGCGAAGTTGTCAAGCTGCTCGACGGTGACGGACGCCCCACTCCCTTCT
ACGGTACAAACATCCACGACAACTGTCCGTACCTGCCGCAGTTTGAAGAATACGTCATGTCTGAAACATTTACTCAGAAA
GACGGCTGCCGGTACGAACTGGGATGTAAAGGCCCCTCCACCATGGCAGACTGCTACAAGCGCAAATGGAACAACGGCGT
CAACTGGTGCATCAGCAACGCTGTCTGCATCGGCTGTGTGGAACCCGACTTCCCCGACGGCAAGTCCCCGTTCTACGAGT
CTTAA

Upstream 100 bases:

>100_bases
CATAAACGCCCAAAGTTTGAAGGAGGCAGGGATGAGTCTCAGCAGGCGTAATTTTGTAAAACTGTGCACAGGCACAGTGG
CGGGCTTTGGGGTATCCCAG

Downstream 100 bases:

>100_bases
CAAGCGCTTGACCAACATCAGGAGGAGACCACATGTCTGGTTGCACGAATAAAATGGCAGCCGGTGGCGTGAGTGGCAAA
ACTAAAATTGCCATCGACCC

Product: hydrogenase (NiFe) small subunit (hydA)

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MFHPAIRDALADTLKGDRPPVLWLQGQGCTGCSVSLLNSVHPSIKDVLLDVISLEFHPTVMAWEGEHAIEHMMKIAEKYE
GKFFVVVEGSIPLAADGKYCIIGDIDHKEISIADAMRDIAAKSAAVLAVGTCAAYGGIPAAEGNLTEAVSVQQFFEQEGI
KTPVVNIPGCPPHPDWIVGTVVVALDAIKKHGLQGGLGEVVKLLDGDGRPTPFYGTNIHDNCPYLPQFEEYVMSETFTQK
DGCRYELGCKGPSTMADCYKRKWNNGVNWCISNAVCIGCVEPDFPDGKSPFYES

Sequences:

>Translated_294_residues
MFHPAIRDALADTLKGDRPPVLWLQGQGCTGCSVSLLNSVHPSIKDVLLDVISLEFHPTVMAWEGEHAIEHMMKIAEKYE
GKFFVVVEGSIPLAADGKYCIIGDIDHKEISIADAMRDIAAKSAAVLAVGTCAAYGGIPAAEGNLTEAVSVQQFFEQEGI
KTPVVNIPGCPPHPDWIVGTVVVALDAIKKHGLQGGLGEVVKLLDGDGRPTPFYGTNIHDNCPYLPQFEEYVMSETFTQK
DGCRYELGCKGPSTMADCYKRKWNNGVNWCISNAVCIGCVEPDFPDGKSPFYES
>Mature_294_residues
MFHPAIRDALADTLKGDRPPVLWLQGQGCTGCSVSLLNSVHPSIKDVLLDVISLEFHPTVMAWEGEHAIEHMMKIAEKYE
GKFFVVVEGSIPLAADGKYCIIGDIDHKEISIADAMRDIAAKSAAVLAVGTCAAYGGIPAAEGNLTEAVSVQQFFEQEGI
KTPVVNIPGCPPHPDWIVGTVVVALDAIKKHGLQGGLGEVVKLLDGDGRPTPFYGTNIHDNCPYLPQFEEYVMSETFTQK
DGCRYELGCKGPSTMADCYKRKWNNGVNWCISNAVCIGCVEPDFPDGKSPFYES

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=282, Percent_Identity=35.8156028368794, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789371, Length=271, Percent_Identity=36.90036900369, Blast_Score=155, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 31862; Mature: 31862

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHPAIRDALADTLKGDRPPVLWLQGQGCTGCSVSLLNSVHPSIKDVLLDVISLEFHPTV
CCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCEE
MAWEGEHAIEHMMKIAEKYEGKFFVVVEGSIPLAADGKYCIIGDIDHKEISIADAMRDIA
EEECCHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCEEEEECCCCCHHHHHHHHHHHH
AKSAAVLAVGTCAAYGGIPAAEGNLTEAVSVQQFFEQEGIKTPVVNIPGCPPHPDWIVGT
HHHHHEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHH
VVVALDAIKKHGLQGGLGEVVKLLDGDGRPTPFYGTNIHDNCPYLPQFEEYVMSETFTQK
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DGCRYELGCKGPSTMADCYKRKWNNGVNWCISNAVCIGCVEPDFPDGKSPFYES
CCCEEEECCCCCHHHHHHHHHHCCCCHHEEECCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MFHPAIRDALADTLKGDRPPVLWLQGQGCTGCSVSLLNSVHPSIKDVLLDVISLEFHPTV
CCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCEE
MAWEGEHAIEHMMKIAEKYEGKFFVVVEGSIPLAADGKYCIIGDIDHKEISIADAMRDIA
EEECCHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCEEEEECCCCCHHHHHHHHHHHH
AKSAAVLAVGTCAAYGGIPAAEGNLTEAVSVQQFFEQEGIKTPVVNIPGCPPHPDWIVGT
HHHHHEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHH
VVVALDAIKKHGLQGGLGEVVKLLDGDGRPTPFYGTNIHDNCPYLPQFEEYVMSETFTQK
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DGCRYELGCKGPSTMADCYKRKWNNGVNWCISNAVCIGCVEPDFPDGKSPFYES
CCCEEEECCCCCHHHHHHHHHHCCCCHHEEECCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]