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The map label for this gene is rho

Identifier: 78357173

GI number: 78357173

Start: 2178283

End: 2179794

Strand: Direct

Name: rho

Synonym: Dde_2130

Alternate gene names: 78357173

Gene position: 2178283-2179794 (Clockwise)

Preceding gene: 78357172

Following gene: 78357177

Centisome position: 58.4

GC content: 50.53

Gene sequence:

>1512_bases
ATGTGCACCGACTCATATTACGAGTTTCCTTTATCCTGTCCTAACCAGATACACCTTGCTTACCATACGGCTCTCCTGAC
GGAACGCTGTAGTTCGGGCTTTTACCTGAGCACTGCACAATGTGCGGCACTGTATCTGGTGCTTTTCTCACCTTCAGCCC
CCCTGCGTCTTGCAATGGAACAGCAAGAAAACACAAAACGAAAATACACTCGCAAGAAGAACACCGTACAGCATCAACCG
CCTGCAAACCCGGACAGTCATATGTATTTGTCTGAGCTGAAAACAAAAAGCATGGCGGAACTGATGGAGCTGGCAGACCA
GTATAACGTCGAAAATCCGAGTAACCTGCGCAAGCAGGAACTTATTTTCGCCCTGCTGCAGAACTGTGCTTCACAAAACG
GTGCCATCTACGGTGAAGGCGTTCTGGAAATTCTGCCCGACGGATTCGGTTTTTTACGCTCGCCCATGTACAGCTACATG
CCGGGTCCGGACGACATTTATGTTTCTCCTTCGCAGATCAGAAGATTTACCCTACGCAAAGGTGACATAGTTTCCGGACA
GATACGGCCTCCGAAAGAGGGAGAGCGATACTTCGCTCTGCTTAAAGTAACCGAAATCGGATGCCAGCCCCCCGAAAAGG
CAAAAAATCTTGTCCTTTTCGACAACCTGACCCCCGTTTACCCTGACAGACAGCTTATTATGGAAAACGGGGACAAAAAC
TTCTCAGGCAGGGTCATAGACCTTATGGCACCCATCGGGTGCGGGCAACGCGGCCTTATTGTCGCTCCGCCCAGAACAGG
TAAAACCGTCCTGCTCCAGAACCTCGCCAACTCGATCAACGCCAACAATCCTGACGTCTACCTCATTGTCCTGCTTATAG
ACGAGCGCCCCGAAGAAGTTACCGACATGGAGCGCACCGTCAAAAATGCCGAAGTCATCAGCTCCACGTTTGACGAACCG
CCCCAGAGGCATGTTCAGGTGTGTGAAATGGTACTGGAAAAGGCAAAGCGTCTGGTCGAACGCAAACGTGACGTCGTGAT
TCTTCTTGATTCCATAACCCGTCTGGGGCGCGCATATAATGCTGTAACCCCCTCTTCCGGCAGGGTTCTTTCCGGCGGAC
TAGACGCCAACGCCCTGCAGCGTCCCAAGCGGTTTTTCGGTGCTGCGCGCAATATTGAAGAAGGCGGAAGCCTCACTATC
ATCGCCACAGCTCTGATCGACACCGGTTCCCGTATGGACGAAGTGATCTTTGAAGAATTCAAAGGCACAGGCAACCTTGA
TATTTATCTTGATCGCCACCTTTCTGAAAAACGCGTATTCCCGGCCATTGATATCAACAGAACCGGTACGCGCAAAGAAG
ATCTTCTGCTTTCGGATGAAGTGCTCAACAGAGTGTGGATTCTGCGCAAAATTCTGGCACCCATGAACTCTGTCGACAGT
ATGGAGTTTTTGCTGGACAAAATGCGGGGTACCAAAAGCAATCGGGACTTTCTTATCGGGATGAATAAATAA

Upstream 100 bases:

>100_bases
AAAATCCCGAATAGCTTCTTGCCAAGCCCGCTGCTTTAGGCTAGGCTTTTCAGAATAGGCCACCATTTTGGCTGCCGCAC
TCTCCACCCGTTCAGGTGGC

Downstream 100 bases:

>100_bases
TACCCGCCTCTTAGCCCGCTCTTATCCAGAGCGGGCTTTTTTTTGGGGTGACTCCGACCTCCGACCGGTAAACCGGCAGT
TACGGTCCGGCTGAGCTGCA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MCTDSYYEFPLSCPNQIHLAYHTALLTERCSSGFYLSTAQCAALYLVLFSPSAPLRLAMEQQENTKRKYTRKKNTVQHQP
PANPDSHMYLSELKTKSMAELMELADQYNVENPSNLRKQELIFALLQNCASQNGAIYGEGVLEILPDGFGFLRSPMYSYM
PGPDDIYVSPSQIRRFTLRKGDIVSGQIRPPKEGERYFALLKVTEIGCQPPEKAKNLVLFDNLTPVYPDRQLIMENGDKN
FSGRVIDLMAPIGCGQRGLIVAPPRTGKTVLLQNLANSINANNPDVYLIVLLIDERPEEVTDMERTVKNAEVISSTFDEP
PQRHVQVCEMVLEKAKRLVERKRDVVILLDSITRLGRAYNAVTPSSGRVLSGGLDANALQRPKRFFGAARNIEEGGSLTI
IATALIDTGSRMDEVIFEEFKGTGNLDIYLDRHLSEKRVFPAIDINRTGTRKEDLLLSDEVLNRVWILRKILAPMNSVDS
MEFLLDKMRGTKSNRDFLIGMNK

Sequences:

>Translated_503_residues
MCTDSYYEFPLSCPNQIHLAYHTALLTERCSSGFYLSTAQCAALYLVLFSPSAPLRLAMEQQENTKRKYTRKKNTVQHQP
PANPDSHMYLSELKTKSMAELMELADQYNVENPSNLRKQELIFALLQNCASQNGAIYGEGVLEILPDGFGFLRSPMYSYM
PGPDDIYVSPSQIRRFTLRKGDIVSGQIRPPKEGERYFALLKVTEIGCQPPEKAKNLVLFDNLTPVYPDRQLIMENGDKN
FSGRVIDLMAPIGCGQRGLIVAPPRTGKTVLLQNLANSINANNPDVYLIVLLIDERPEEVTDMERTVKNAEVISSTFDEP
PQRHVQVCEMVLEKAKRLVERKRDVVILLDSITRLGRAYNAVTPSSGRVLSGGLDANALQRPKRFFGAARNIEEGGSLTI
IATALIDTGSRMDEVIFEEFKGTGNLDIYLDRHLSEKRVFPAIDINRTGTRKEDLLLSDEVLNRVWILRKILAPMNSVDS
MEFLLDKMRGTKSNRDFLIGMNK
>Mature_503_residues
MCTDSYYEFPLSCPNQIHLAYHTALLTERCSSGFYLSTAQCAALYLVLFSPSAPLRLAMEQQENTKRKYTRKKNTVQHQP
PANPDSHMYLSELKTKSMAELMELADQYNVENPSNLRKQELIFALLQNCASQNGAIYGEGVLEILPDGFGFLRSPMYSYM
PGPDDIYVSPSQIRRFTLRKGDIVSGQIRPPKEGERYFALLKVTEIGCQPPEKAKNLVLFDNLTPVYPDRQLIMENGDKN
FSGRVIDLMAPIGCGQRGLIVAPPRTGKTVLLQNLANSINANNPDVYLIVLLIDERPEEVTDMERTVKNAEVISSTFDEP
PQRHVQVCEMVLEKAKRLVERKRDVVILLDSITRLGRAYNAVTPSSGRVLSGGLDANALQRPKRFFGAARNIEEGGSLTI
IATALIDTGSRMDEVIFEEFKGTGNLDIYLDRHLSEKRVFPAIDINRTGTRKEDLLLSDEVLNRVWILRKILAPMNSVDS
MEFLLDKMRGTKSNRDFLIGMNK

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=64.9164677804296, Blast_Score=568, Evalue=1e-163,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 56647; Mature: 56647

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTDSYYEFPLSCPNQIHLAYHTALLTERCSSGFYLSTAQCAALYLVLFSPSAPLRLAME
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEHHHHHHEEHHEECCCCCEEEHHH
QQENTKRKYTRKKNTVQHQPPANPDSHMYLSELKTKSMAELMELADQYNVENPSNLRKQE
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LIFALLQNCASQNGAIYGEGVLEILPDGFGFLRSPMYSYMPGPDDIYVSPSQIRRFTLRK
HHHHHHHHHHCCCCCEEECCHHHHCCCCHHHHHCCHHHCCCCCCCEEECHHHHHHHHCCC
GDIVSGQIRPPKEGERYFALLKVTEIGCQPPEKAKNLVLFDNLTPVYPDRQLIMENGDKN
CCEEECCCCCCCCCCCEEEEEEEEECCCCCCHHHCCEEEECCCCCCCCCHHHHEECCCCC
FSGRVIDLMAPIGCGQRGLIVAPPRTGKTVLLQNLANSINANNPDVYLIVLLIDERPEEV
CCCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHH
TDMERTVKNAEVISSTFDEPPQRHVQVCEMVLEKAKRLVERKRDVVILLDSITRLGRAYN
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHC
AVTPSSGRVLSGGLDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEF
CCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
KGTGNLDIYLDRHLSEKRVFPAIDINRTGTRKEDLLLSDEVLNRVWILRKILAPMNSVDS
CCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MEFLLDKMRGTKSNRDFLIGMNK
HHHHHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure
MCTDSYYEFPLSCPNQIHLAYHTALLTERCSSGFYLSTAQCAALYLVLFSPSAPLRLAME
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEHHHHHHEEHHEECCCCCEEEHHH
QQENTKRKYTRKKNTVQHQPPANPDSHMYLSELKTKSMAELMELADQYNVENPSNLRKQE
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LIFALLQNCASQNGAIYGEGVLEILPDGFGFLRSPMYSYMPGPDDIYVSPSQIRRFTLRK
HHHHHHHHHHCCCCCEEECCHHHHCCCCHHHHHCCHHHCCCCCCCEEECHHHHHHHHCCC
GDIVSGQIRPPKEGERYFALLKVTEIGCQPPEKAKNLVLFDNLTPVYPDRQLIMENGDKN
CCEEECCCCCCCCCCCEEEEEEEEECCCCCCHHHCCEEEECCCCCCCCCHHHHEECCCCC
FSGRVIDLMAPIGCGQRGLIVAPPRTGKTVLLQNLANSINANNPDVYLIVLLIDERPEEV
CCCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHH
TDMERTVKNAEVISSTFDEPPQRHVQVCEMVLEKAKRLVERKRDVVILLDSITRLGRAYN
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHC
AVTPSSGRVLSGGLDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEF
CCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
KGTGNLDIYLDRHLSEKRVFPAIDINRTGTRKEDLLLSDEVLNRVWILRKILAPMNSVDS
CCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MEFLLDKMRGTKSNRDFLIGMNK
HHHHHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]