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The map label for this gene is aes [H]

Identifier: 78357055

GI number: 78357055

Start: 2053573

End: 2054550

Strand: Reverse

Name: aes [H]

Synonym: Dde_2012

Alternate gene names: 78357055

Gene position: 2054550-2053573 (Counterclockwise)

Preceding gene: 78357059

Following gene: 78357053

Centisome position: 55.08

GC content: 61.25

Gene sequence:

>978_bases
ATGGCGAACGAATCGGCAACGATACCCGTCACCTGCATTGAAGGGTATGACACTATGGACGAGGAAACCGGCGCGTTTCT
GGCCGCAGCCGCGGGCAGCGGCGTGCCGCTTGCCCGGCAGGATATTGCAGACATACGAAATGATTATGAACAGAGGCTGA
GGACAGGTATTGAGCAGGCTCCGGCCATGGCCGCGGTGGAAGAGCATGCCGTGCCTGTCTTCCGCGGCTCGATTCCGCTG
AGGATATACCGTCCGGTTGCTGATAGCTGCCTGCCGGTGATTTTGTTTCTGCACGGTGGTGGGTTTGTTAAGGGAAGCAT
TGACTCGCATGAAGCCATGTGCCGCGAGCTTGCCTTGCGCAGCGGGCTGGCCGTCGCCGCTGCGGGGTATCGCCTTGCAC
CGGAGCATCGCTATCCGGTGGCTCAGGACGACTGTATCTGCGCTTTGCACTGGCTGGGCGCCCATGCGGCAGAAGCCGGG
GTCAACGGTGGCAGAATGGTGGTCGCGGGCGATAGTGCAGGAGGCCAGCTGGCTGCCGCGCTGGCTGTGTATGCCCGGGA
TGCGGGGCTGGAGGCGTTGCGGGGGCAGATTCTTTTTTATCCGTGCGTCGATATGGCAAGCAGCGGTGCGTCACACAATC
AGTGTGCTACCGGATTCGGCCTTACGGAAGAGTCTGTCCGCTGGTATTATAACGCCTACCTGCCGCAGGGAATGGCCTGT
GATGATCCTGCCGTGTCTCCTCTTTATACAGAGAATCTGCAGGGCGTGGCTCCGGCGGCTGTGTTTAATGCTGTTTATGA
TCCGGTGCGGTCAGACGGCGAACTGTTTGTGCAGCGGCTGCGCGCGGCCGGCGTTTCCGCAAGGCTGGCTCTGTATCCCA
CGGTGCATGGCTTTGTCAGTACATACACGCGGTTCCATGCAGGCCGCAGGGCGCTGGCTGCAGCGGGAGCATTCGCCGCG
GAGGTGACAGCAGGGTGA

Upstream 100 bases:

>100_bases
TGGCAATACGTAACGATAATAGAGCTTATTTTTGTGTTGTAAACCACCGTGCTGCATATCAGACGCTGTCTGATACGACA
TATCTGTACAGGAGGCCGTC

Downstream 100 bases:

>100_bases
TGTGCACAAAAAAAGGGGGCCTGCGGCCCCCTTTGTCATTATCCGTGACGGCTACTTTTTGTTAAGTGCGTCTTTAAGCG
CCTGCCCCAGTGTGCCGAAT

Product: putative acetyl hydrolace

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MANESATIPVTCIEGYDTMDEETGAFLAAAAGSGVPLARQDIADIRNDYEQRLRTGIEQAPAMAAVEEHAVPVFRGSIPL
RIYRPVADSCLPVILFLHGGGFVKGSIDSHEAMCRELALRSGLAVAAAGYRLAPEHRYPVAQDDCICALHWLGAHAAEAG
VNGGRMVVAGDSAGGQLAAALAVYARDAGLEALRGQILFYPCVDMASSGASHNQCATGFGLTEESVRWYYNAYLPQGMAC
DDPAVSPLYTENLQGVAPAAVFNAVYDPVRSDGELFVQRLRAAGVSARLALYPTVHGFVSTYTRFHAGRRALAAAGAFAA
EVTAG

Sequences:

>Translated_325_residues
MANESATIPVTCIEGYDTMDEETGAFLAAAAGSGVPLARQDIADIRNDYEQRLRTGIEQAPAMAAVEEHAVPVFRGSIPL
RIYRPVADSCLPVILFLHGGGFVKGSIDSHEAMCRELALRSGLAVAAAGYRLAPEHRYPVAQDDCICALHWLGAHAAEAG
VNGGRMVVAGDSAGGQLAAALAVYARDAGLEALRGQILFYPCVDMASSGASHNQCATGFGLTEESVRWYYNAYLPQGMAC
DDPAVSPLYTENLQGVAPAAVFNAVYDPVRSDGELFVQRLRAAGVSARLALYPTVHGFVSTYTRFHAGRRALAAAGAFAA
EVTAG
>Mature_324_residues
ANESATIPVTCIEGYDTMDEETGAFLAAAAGSGVPLARQDIADIRNDYEQRLRTGIEQAPAMAAVEEHAVPVFRGSIPLR
IYRPVADSCLPVILFLHGGGFVKGSIDSHEAMCRELALRSGLAVAAAGYRLAPEHRYPVAQDDCICALHWLGAHAAEAGV
NGGRMVVAGDSAGGQLAAALAVYARDAGLEALRGQILFYPCVDMASSGASHNQCATGFGLTEESVRWYYNAYLPQGMACD
DPAVSPLYTENLQGVAPAAVFNAVYDPVRSDGELFVQRLRAAGVSARLALYPTVHGFVSTYTRFHAGRRALAAAGAFAAE
VTAG

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=149, Percent_Identity=38.255033557047, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI61966717, Length=148, Percent_Identity=36.4864864864865, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI68299767, Length=277, Percent_Identity=25.2707581227437, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI206597554, Length=274, Percent_Identity=25.1824817518248, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI21328446, Length=111, Percent_Identity=36.9369369369369, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=281, Percent_Identity=30.9608540925267, Blast_Score=118, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17540028, Length=176, Percent_Identity=34.6590909090909, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71996133, Length=182, Percent_Identity=31.3186813186813, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI72001146, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17567059, Length=157, Percent_Identity=29.9363057324841, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24656084, Length=119, Percent_Identity=36.1344537815126, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24656076, Length=119, Percent_Identity=36.1344537815126, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130169, Length=119, Percent_Identity=36.1344537815126, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34210; Mature: 34079

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANESATIPVTCIEGYDTMDEETGAFLAAAAGSGVPLARQDIADIRNDYEQRLRTGIEQA
CCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PAMAAVEEHAVPVFRGSIPLRIYRPVADSCLPVILFLHGGGFVKGSIDSHEAMCRELALR
HHHHHHHHHCCEEEECCCCEEEECCHHHHHHHEEHEEECCCEEECCCCHHHHHHHHHHHH
SGLAVAAAGYRLAPEHRYPVAQDDCICALHWLGAHAAEAGVNGGRMVVAGDSAGGQLAAA
CCCEEEECCCEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
LAVYARDAGLEALRGQILFYPCVDMASSGASHNQCATGFGLTEESVRWYYNAYLPQGMAC
HHHHHHHCCHHHHCCCEEEEECHHHHCCCCCCCCCCCCCCCCHHHHHHEEEECCCCCCCC
DDPAVSPLYTENLQGVAPAAVFNAVYDPVRSDGELFVQRLRAAGVSARLALYPTVHGFVS
CCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHH
TYTRFHAGRRALAAAGAFAAEVTAG
HHHHHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure 
ANESATIPVTCIEGYDTMDEETGAFLAAAAGSGVPLARQDIADIRNDYEQRLRTGIEQA
CCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PAMAAVEEHAVPVFRGSIPLRIYRPVADSCLPVILFLHGGGFVKGSIDSHEAMCRELALR
HHHHHHHHHCCEEEECCCCEEEECCHHHHHHHEEHEEECCCEEECCCCHHHHHHHHHHHH
SGLAVAAAGYRLAPEHRYPVAQDDCICALHWLGAHAAEAGVNGGRMVVAGDSAGGQLAAA
CCCEEEECCCEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
LAVYARDAGLEALRGQILFYPCVDMASSGASHNQCATGFGLTEESVRWYYNAYLPQGMAC
HHHHHHHCCHHHHCCCEEEEECHHHHCCCCCCCCCCCCCCCCHHHHHHEEEECCCCCCCC
DDPAVSPLYTENLQGVAPAAVFNAVYDPVRSDGELFVQRLRAAGVSARLALYPTVHGFVS
CCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHH
TYTRFHAGRRALAAAGAFAAEVTAG
HHHHHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA