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The map label for this gene is rpfG [H]

Identifier: 78356985

GI number: 78356985

Start: 1990462

End: 1991553

Strand: Reverse

Name: rpfG [H]

Synonym: Dde_1942

Alternate gene names: 78356985

Gene position: 1991553-1990462 (Counterclockwise)

Preceding gene: 78356986

Following gene: 78356984

Centisome position: 53.39

GC content: 51.1

Gene sequence:

>1092_bases
ATGATGAAAAGAACCAACCGGATTCTCATAGTTGATGACAACCATGTGAATATTCTTATCCTTAAGCAGATTCTGGAACA
GGAGCATCAGGTGTTTGCTGCTGTAAACGGATACGAGGCTCTTGAACTGGCCTCGGGGCCGATGAAGCCCGACATTATTC
TGCTGGATGTGCTGATGCCTGAAATGGACGGGTACGAAGTGTGCCGCAGATTAAAGGAAAATGACGAGACGCGCGATATT
CCTGTTCTTTTTGTAACTACACAGGGAGAAGAAGAGGATGAGGCTGCCGGTCTTGCTCTGGGTGCTGTGGATTACATAGT
CAAGCCGGTACGCCCTTCCATAGTGCTTGCGCGGGTAAGGAATCATCTGCAACTGAAGACGCATCAGGATCAGCTGGAAG
ATCTGGTGCGGGACAGAACCCGTGAGCTGCAGCTGACTCAGGATGTGACTATTTTTTCATTGGCCTGCCTTGCCGAAATG
CGTGACAACGAACTTGGTGGGCATTTTAAGCGGACGCAGAACTATGTCCGCCTGCTTGCTTCCGCCGCCAAGGCCAAAGG
TGCCTATAAGGAATATTGGCGTCAGATTTCTGTAAGTATGCTGCACAAATCTGCCCCCCTGCATGACGTGGGCAAGGTTG
GCGTACCCGACAATATTCTGCTGAAGCCGGGCAAGCTTACAGAAGAAGAATTTGCCATCATGAAGCTGCATACGGTGTAT
GGCAGGGACTCTCTGGAGCGAGCGGAGTCGTTGCTGCAGTACAAGTCTTTTCTGAGCGTTGCCAAAGAAATAGCTTATTC
GCATCACGAGCGGTGGGACGGCCGTGGCTACCCTGAAGGACTTGCCGGCGATGCTATTCCCGTTTCGGGCAGGATTATGG
CTGTTGCCGATGTGTATGACGCACTTATCAGCAAAAGAGTGTATAAAGATCCCCTTCCCCATTCCACTGCCGTGCAGATA
GTGCTGGAGGGCAGCGGCAATCATTTTGATCCTGTGTTGTGTGAACTGTTTGAACGACATCAGGAAGAAATCCGCGCCAT
AGCCATCGCAGACGCAGATCTGGAAGAAGAGCGTGAGGCATTGAGGAGATGA

Upstream 100 bases:

>100_bases
ACGATGATCTTGTCCGCGCGCTGAATAAGTTCGACTTTAAGGCTGCGCTGAAGCTTCTTGACCGTATACATCTGCAAACC
GAAGAGAACGGGATGGTGCA

Downstream 100 bases:

>100_bases
CCGTTCAGGGCGGTGCCATATGATACATACCGCAGAACGCTTTTCCGTGCTTTGTGTGGATGATATGCCAGCCAATCTTG
CCGTTCTTGATGGTATCCTG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MMKRTNRILIVDDNHVNILILKQILEQEHQVFAAVNGYEALELASGPMKPDIILLDVLMPEMDGYEVCRRLKENDETRDI
PVLFVTTQGEEEDEAAGLALGAVDYIVKPVRPSIVLARVRNHLQLKTHQDQLEDLVRDRTRELQLTQDVTIFSLACLAEM
RDNELGGHFKRTQNYVRLLASAAKAKGAYKEYWRQISVSMLHKSAPLHDVGKVGVPDNILLKPGKLTEEEFAIMKLHTVY
GRDSLERAESLLQYKSFLSVAKEIAYSHHERWDGRGYPEGLAGDAIPVSGRIMAVADVYDALISKRVYKDPLPHSTAVQI
VLEGSGNHFDPVLCELFERHQEEIRAIAIADADLEEEREALRR

Sequences:

>Translated_363_residues
MMKRTNRILIVDDNHVNILILKQILEQEHQVFAAVNGYEALELASGPMKPDIILLDVLMPEMDGYEVCRRLKENDETRDI
PVLFVTTQGEEEDEAAGLALGAVDYIVKPVRPSIVLARVRNHLQLKTHQDQLEDLVRDRTRELQLTQDVTIFSLACLAEM
RDNELGGHFKRTQNYVRLLASAAKAKGAYKEYWRQISVSMLHKSAPLHDVGKVGVPDNILLKPGKLTEEEFAIMKLHTVY
GRDSLERAESLLQYKSFLSVAKEIAYSHHERWDGRGYPEGLAGDAIPVSGRIMAVADVYDALISKRVYKDPLPHSTAVQI
VLEGSGNHFDPVLCELFERHQEEIRAIAIADADLEEEREALRR
>Mature_363_residues
MMKRTNRILIVDDNHVNILILKQILEQEHQVFAAVNGYEALELASGPMKPDIILLDVLMPEMDGYEVCRRLKENDETRDI
PVLFVTTQGEEEDEAAGLALGAVDYIVKPVRPSIVLARVRNHLQLKTHQDQLEDLVRDRTRELQLTQDVTIFSLACLAEM
RDNELGGHFKRTQNYVRLLASAAKAKGAYKEYWRQISVSMLHKSAPLHDVGKVGVPDNILLKPGKLTEEEFAIMKLHTVY
GRDSLERAESLLQYKSFLSVAKEIAYSHHERWDGRGYPEGLAGDAIPVSGRIMAVADVYDALISKRVYKDPLPHSTAVQI
VLEGSGNHFDPVLCELFERHQEEIRAIAIADADLEEEREALRR

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=129, Percent_Identity=34.1085271317829, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=40, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788550, Length=135, Percent_Identity=34.8148148148148, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788394, Length=130, Percent_Identity=35.3846153846154, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=34.1463414634146, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082012, Length=121, Percent_Identity=36.3636363636364, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786784, Length=139, Percent_Identity=33.8129496402878, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790863, Length=122, Percent_Identity=34.4262295081967, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 41110; Mature: 41110

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRTNRILIVDDNHVNILILKQILEQEHQVFAAVNGYEALELASGPMKPDIILLDVLMP
CCCCCCEEEEEECCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCC
EMDGYEVCRRLKENDETRDIPVLFVTTQGEEEDEAAGLALGAVDYIVKPVRPSIVLARVR
CCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NHLQLKTHQDQLEDLVRDRTRELQLTQDVTIFSLACLAEMRDNELGGHFKRTQNYVRLLA
HHHEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SAAKAKGAYKEYWRQISVSMLHKSAPLHDVGKVGVPDNILLKPGKLTEEEFAIMKLHTVY
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
GRDSLERAESLLQYKSFLSVAKEIAYSHHERWDGRGYPEGLAGDAIPVSGRIMAVADVYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEHHHHHHH
ALISKRVYKDPLPHSTAVQIVLEGSGNHFDPVLCELFERHQEEIRAIAIADADLEEEREA
HHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHHH
LRR
HCC
>Mature Secondary Structure
MMKRTNRILIVDDNHVNILILKQILEQEHQVFAAVNGYEALELASGPMKPDIILLDVLMP
CCCCCCEEEEEECCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCC
EMDGYEVCRRLKENDETRDIPVLFVTTQGEEEDEAAGLALGAVDYIVKPVRPSIVLARVR
CCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NHLQLKTHQDQLEDLVRDRTRELQLTQDVTIFSLACLAEMRDNELGGHFKRTQNYVRLLA
HHHEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SAAKAKGAYKEYWRQISVSMLHKSAPLHDVGKVGVPDNILLKPGKLTEEEFAIMKLHTVY
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
GRDSLERAESLLQYKSFLSVAKEIAYSHHERWDGRGYPEGLAGDAIPVSGRIMAVADVYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEHHHHHHH
ALISKRVYKDPLPHSTAVQIVLEGSGNHFDPVLCELFERHQEEIRAIAIADADLEEEREA
HHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHHH
LRR
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]