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The map label for this gene is aspA

Identifier: 78356706

GI number: 78356706

Start: 1716328

End: 1717752

Strand: Direct

Name: aspA

Synonym: Dde_1663

Alternate gene names: 78356706

Gene position: 1716328-1717752 (Clockwise)

Preceding gene: 78356705

Following gene: 78356707

Centisome position: 46.01

GC content: 53.47

Gene sequence:

>1425_bases
ATGACCTACCGTACGGAACATGACAGTCTTGGCGAACGCAAGGTTCCCGCACAGGCTTACTACGGCATTCAGACACTGAG
GGCTCTGGAGAATTTTGAAATCACAACTATTCCTGTTTCGCATTACAGCAGGTTCATACAGGCGCTGGCTGTGGTTAAAA
AAGCTGCGGCCATGGCCAATATGGAGTTGGGAGAACTCGATTCCGCCGTCGGCGAAGCTATTATCCATGCCTGCAAAGAC
GTGCGGCAGGGCAAGCTGGATGACCAGTTTGTAGTGGATGTCATTCAGGGCGGGGCGGGAACGTCGGTAAACATGAACGC
CAATGAAGTGATCTGCAACAGGGCTCTTGAGCTGAGCGGGCATGAAAAAGGGAGGTATGACATTATCCATCCGCTCAATC
ATGTGAATCTTTCGCAGTCCACCAATGATGTTTACCCCACGGCTTTGCGCATAGCGCTTGTGTGGTACAGCCGCGAACTG
CTGCACGGGCTGCGCGAACTGCGCGACGCTTTGAGCCGCAAAGGTGCAGAATTCGGCAATGTCATTAAAATGGCCCGCAC
ACAGCTGCAGGATGCCGTTCCCATCACGCTCGGAGCCGAGTTTGCGGCATATGCTGTTACCGTCAATGAAGACATTGACC
GTCTGGAAGAGGTGATGCGCCTGCTTTGCGAAGTCAATCTGGGGGCTACGGCAGTGGGAACAGGCATCAATTCCGTTCCG
GGCTACGGAGCCCGCGCCTGCGCCATTCTGGCTGATGTGACAGGCCTGCCCATATCTCAGTCGGCGAATCTGGTAGAAGC
AACCAGCGATGCCGGCGCATATGTGACTCTTTCAGGACTGCTGAAACGCACCAGTGTGAAACTTTCAAAGATATGTAATG
ATCTCAGATTGATGTCTTCAGGGCCGTTTACCGGCCTGCACGAAATCAATCTGCCGCCGGTGCAGCCCGGATCATCCATA
ATGCCGGGCAAGGTCAACCCTGTCATTCCTGAAGTGGTCACCCAGATATGCTACCAGATTATCGGCAATGACATGACAGT
GACCATGGCTGCGGAGGCAGGGCAGCTTGAGCTTAATGTTTTCGGACCCATTATCGCATTTAATCTGTTTCAGTCACTGG
AAATTCTGACCAGAGGTGTGCACACCCTGAGAAAGCGGTGCATCGAGGGCATAACCGCTAATGATGAGCGTTGTCTTGAA
TTGCTCAAAGGTTCTCTGGGGGTGGTGACGGCTTTAGCTCCCGCCATAGGATATGAGGCGGCTGCAAAAGTGGTCAAACA
GGCACAGGAAACGCGCAAGCCTGTGGGACAGGTGCTTGACGAGCAGGGGCTTATGACCGCCGAAGAGTATAACGAGCTGC
TGGATCCGAGCAAGATGCTTGCTCCGAGAAAGTTGCCGAACCGTAAAGCGGCAGAGGATAAATGA

Upstream 100 bases:

>100_bases
TTTTTTTGCGGTTGTCAGATGTGTTTGTGTTGCAATAAGCTGCTGTGGCACGTGGAAACCGGTATTTTTATCCAACCCCA
CCCGGAAACAGCGAGGCAGC

Downstream 100 bases:

>100_bases
CCAGAACTGCTTTCTGCTGCTCGCATCCTGTTTCTTCCGCCATGCCGCACTGGCCGGAAGATCCGCCCACTGTGTTCACT
CCGTGGTCGGACATGAACAC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTYRTEHDSLGERKVPAQAYYGIQTLRALENFEITTIPVSHYSRFIQALAVVKKAAAMANMELGELDSAVGEAIIHACKD
VRQGKLDDQFVVDVIQGGAGTSVNMNANEVICNRALELSGHEKGRYDIIHPLNHVNLSQSTNDVYPTALRIALVWYSREL
LHGLRELRDALSRKGAEFGNVIKMARTQLQDAVPITLGAEFAAYAVTVNEDIDRLEEVMRLLCEVNLGATAVGTGINSVP
GYGARACAILADVTGLPISQSANLVEATSDAGAYVTLSGLLKRTSVKLSKICNDLRLMSSGPFTGLHEINLPPVQPGSSI
MPGKVNPVIPEVVTQICYQIIGNDMTVTMAAEAGQLELNVFGPIIAFNLFQSLEILTRGVHTLRKRCIEGITANDERCLE
LLKGSLGVVTALAPAIGYEAAAKVVKQAQETRKPVGQVLDEQGLMTAEEYNELLDPSKMLAPRKLPNRKAAEDK

Sequences:

>Translated_474_residues
MTYRTEHDSLGERKVPAQAYYGIQTLRALENFEITTIPVSHYSRFIQALAVVKKAAAMANMELGELDSAVGEAIIHACKD
VRQGKLDDQFVVDVIQGGAGTSVNMNANEVICNRALELSGHEKGRYDIIHPLNHVNLSQSTNDVYPTALRIALVWYSREL
LHGLRELRDALSRKGAEFGNVIKMARTQLQDAVPITLGAEFAAYAVTVNEDIDRLEEVMRLLCEVNLGATAVGTGINSVP
GYGARACAILADVTGLPISQSANLVEATSDAGAYVTLSGLLKRTSVKLSKICNDLRLMSSGPFTGLHEINLPPVQPGSSI
MPGKVNPVIPEVVTQICYQIIGNDMTVTMAAEAGQLELNVFGPIIAFNLFQSLEILTRGVHTLRKRCIEGITANDERCLE
LLKGSLGVVTALAPAIGYEAAAKVVKQAQETRKPVGQVLDEQGLMTAEEYNELLDPSKMLAPRKLPNRKAAEDK
>Mature_473_residues
TYRTEHDSLGERKVPAQAYYGIQTLRALENFEITTIPVSHYSRFIQALAVVKKAAAMANMELGELDSAVGEAIIHACKDV
RQGKLDDQFVVDVIQGGAGTSVNMNANEVICNRALELSGHEKGRYDIIHPLNHVNLSQSTNDVYPTALRIALVWYSRELL
HGLRELRDALSRKGAEFGNVIKMARTQLQDAVPITLGAEFAAYAVTVNEDIDRLEEVMRLLCEVNLGATAVGTGINSVPG
YGARACAILADVTGLPISQSANLVEATSDAGAYVTLSGLLKRTSVKLSKICNDLRLMSSGPFTGLHEINLPPVQPGSSIM
PGKVNPVIPEVVTQICYQIIGNDMTVTMAAEAGQLELNVFGPIIAFNLFQSLEILTRGVHTLRKRCIEGITANDERCLEL
LKGSLGVVTALAPAIGYEAAAKVVKQAQETRKPVGQVLDEQGLMTAEEYNELLDPSKMLAPRKLPNRKAAEDK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=463, Percent_Identity=43.4125269978402, Blast_Score=374, Evalue=1e-103,
Organism=Escherichia coli, GI87082375, Length=472, Percent_Identity=52.1186440677966, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1787896, Length=466, Percent_Identity=40.343347639485, Blast_Score=346, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17553882, Length=470, Percent_Identity=42.3404255319149, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565146, Length=331, Percent_Identity=40.785498489426, Blast_Score=262, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6324993, Length=463, Percent_Identity=43.4125269978402, Blast_Score=385, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640177, Length=467, Percent_Identity=43.0406852248394, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640179, Length=467, Percent_Identity=43.0406852248394, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78710009, Length=467, Percent_Identity=42.1841541755889, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=41.7204301075269, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24583245, Length=465, Percent_Identity=38.7096774193548, Blast_Score=328, Evalue=5e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51317; Mature: 51186

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYRTEHDSLGERKVPAQAYYGIQTLRALENFEITTIPVSHYSRFIQALAVVKKAAAMAN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
MELGELDSAVGEAIIHACKDVRQGKLDDQFVVDVIQGGAGTSVNMNANEVICNRALELSG
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHCCC
HEKGRYDIIHPLNHVNLSQSTNDVYPTALRIALVWYSRELLHGLRELRDALSRKGAEFGN
CCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VIKMARTQLQDAVPITLGAEFAAYAVTVNEDIDRLEEVMRLLCEVNLGATAVGTGINSVP
HHHHHHHHHHHCCCEEECCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCC
GYGARACAILADVTGLPISQSANLVEATSDAGAYVTLSGLLKRTSVKLSKICNDLRLMSS
CCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
GPFTGLHEINLPPVQPGSSIMPGKVNPVIPEVVTQICYQIIGNDMTVTMAAEAGQLELNV
CCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEH
FGPIIAFNLFQSLEILTRGVHTLRKRCIEGITANDERCLELLKGSLGVVTALAPAIGYEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHCHHH
AAKVVKQAQETRKPVGQVLDEQGLMTAEEYNELLDPSKMLAPRKLPNRKAAEDK
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TYRTEHDSLGERKVPAQAYYGIQTLRALENFEITTIPVSHYSRFIQALAVVKKAAAMAN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
MELGELDSAVGEAIIHACKDVRQGKLDDQFVVDVIQGGAGTSVNMNANEVICNRALELSG
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHCCC
HEKGRYDIIHPLNHVNLSQSTNDVYPTALRIALVWYSRELLHGLRELRDALSRKGAEFGN
CCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VIKMARTQLQDAVPITLGAEFAAYAVTVNEDIDRLEEVMRLLCEVNLGATAVGTGINSVP
HHHHHHHHHHHCCCEEECCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCC
GYGARACAILADVTGLPISQSANLVEATSDAGAYVTLSGLLKRTSVKLSKICNDLRLMSS
CCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
GPFTGLHEINLPPVQPGSSIMPGKVNPVIPEVVTQICYQIIGNDMTVTMAAEAGQLELNV
CCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEH
FGPIIAFNLFQSLEILTRGVHTLRKRCIEGITANDERCLELLKGSLGVVTALAPAIGYEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHCHHH
AAKVVKQAQETRKPVGQVLDEQGLMTAEEYNELLDPSKMLAPRKLPNRKAAEDK
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]