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The map label for this gene is leuC [H]

Identifier: 78356451

GI number: 78356451

Start: 1422936

End: 1424867

Strand: Direct

Name: leuC [H]

Synonym: Dde_1406

Alternate gene names: 78356451

Gene position: 1422936-1424867 (Clockwise)

Preceding gene: 78356450

Following gene: 78356452

Centisome position: 38.15

GC content: 61.02

Gene sequence:

>1932_bases
ATGACTATGAATCTGACACAAAAGATCATTGCCGCCCATCTTGTAAGCGGCGAGATGACACAGGGCGCCGAAATAGGGCT
GCGCATAGACCAGACGCTGACGCAGGATGCCACCGGCACCATGGCGTATCTGCAGTTTGAAGCCATGGGCGTGGACAGGG
TGCGCACTGACCTGTCGGTCAGCTATGTGGACCACAACACGCTGCAGATGGGATTCCGCAATCCGGATGACCACCGTTTT
CTGCGCACGGTGGCGCAGAAGTACGGCATTGTCTATTCACCGGCGGGCACGGGCATCTGTCATCAGCTGCATCTGGAAAA
TTTTGCGGTGCCCGGTTCCACGCTGGTGGGGTCGGACAGCCACACGCCCACGGCGGGCGGTATAGGTGCCCTTGCCATGG
GGGCCGGCGGGCTTTCCGTTGCTCTGGCCATGGCAGGAGAGCCGTATTTCATCACCATGCCCCGCGTGGTGAAAGTGCGT
CTGGAAGGCCGCCTGAAGGGGTGGGCCACGGCAAAGGATGTCATTCTGCATCTGCTGGGGCTGCTGACAGTCAAGGGCGG
TGTGGGCAAGGTGTTTGAATACGCCGGTCCCGGTGTCGAAACCCTCAGCGTGCCCGAGCGTGCCACCATCACCAACATGG
GGGCCGAACTGGGCGCCACCACGTCGGTGTTCCCCAGCGATGAGCGCACCCGCGATTTTCTGAAGGCCATGGGCCGTGCT
GATGTATGGAAGCCGCTGGCGGCGGATGACGGGGCGCAATACGACGAAGAGGTCGTCATTGACCTTTCGGCACTGCAGCC
GCTGGCTGCGCAGCCGCACATGCCCGACAGGGTGGTGCCGGTGGCCAGTCTGGACGGTCTGAAGGTTGATCAGGTGGCCA
TCGGGTCGTGCACCAATTCATCATACGCCGACCTTAAAGGTGCGGTAAGCCTGCTGGCAGGCAACAGGGTATGCGCCGGC
ACCGATACGCTGGTTTCCCCCGGTTCCAAGCAGGTGCTGAAGATGCTTGCGCGTGAAGGGCTGGTCGAGCCGCTGCTTGA
TTCCGGCGCACGGCTGCTGGAGTGTTCATGCGGGCCGTGTATCGGCATGGGGGGGTCCCCTGTCAGCGGGGGCGTGTCGG
CCCGTACCTTCAACCGCAATTTTGAAGGCCGCAGCGGTACCAAAGACGGGCAGGTTTATCTTGTCAGTCCGCTGACCGCA
GCCATGGCTGCTTTGCGCGGCTGTTTCACCGATCCCGCCACATGGGGAGAAGCTCCCGCGGTGGCCGATCTGCCCGCCGA
AGTCCCTTCCATCGCTGATTTGTTTGCCTATCCGCCGCAGGACGGAGCAGGCACAGAGGTTGTCTACGGGCCCAACATCG
TGGCGCTGGAGCGTTTTGAACCGCTGCCGGAGCAGCTGCAGGCCGGTGTGCTTATCCGGCTGGGTGACGATATAACCACC
GACCACATTATGCCCGCCGGTGCGGAAATAACCTCGCTGCGGTCCAATGTGCCCGCCATTGCGCGGCATGTTTTCGGCCG
GGTGGACGAAGATTTTGTCACGCGGGCCGATGCCTTGGAAAACGGGGTTATCGTGGCAGGTGAAAATTACGGACAGGGAT
CCAGTCGCGAACATGCGGCCCTTGCTCCGCGCCATCTGGGTGTGCGTGCGGTCATAGCCAAGTCGCTTGCCCGTATCCAC
AGAGCCAATCTTGTGAACTTCGGCATTCTGCCGCTGATTCTGGAAGATAAAGCCGACTATGCGCAGCTGGAACAGGGCTG
CACCGTCACTCTGGACACCACGCTGCTGGCTCCGGGAGCCGTCGTGCAGGTAACAGTGGACGGCGGCGCTGCAGTGGCTG
TCAGGAATGATTTGACTGCTAAGGAATTGGACATTATTCGGGCAGGCGGATTGCTTAATTATGTACGTCTCTCGAAAACG
GCTGCTGCTTAG

Upstream 100 bases:

>100_bases
CGTGGCTTTGTGCAAGCCGGACGGGGCGGCATGCTTCCCCCGTGCGGCGGCACGCAGAGCGCGGCAACTTTTTTTATCCG
TGAGTACGAGGGAATTAGCC

Downstream 100 bases:

>100_bases
CGGCGGCCCATCTGCTTTGAAAAAACGGAGTGCTCATGCTGGACTCAATAAGACAGAACGCCCAGTCGTGGGGCGTTAAA
ATCGCCTTCGGGCTTATCAT

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MTMNLTQKIIAAHLVSGEMTQGAEIGLRIDQTLTQDATGTMAYLQFEAMGVDRVRTDLSVSYVDHNTLQMGFRNPDDHRF
LRTVAQKYGIVYSPAGTGICHQLHLENFAVPGSTLVGSDSHTPTAGGIGALAMGAGGLSVALAMAGEPYFITMPRVVKVR
LEGRLKGWATAKDVILHLLGLLTVKGGVGKVFEYAGPGVETLSVPERATITNMGAELGATTSVFPSDERTRDFLKAMGRA
DVWKPLAADDGAQYDEEVVIDLSALQPLAAQPHMPDRVVPVASLDGLKVDQVAIGSCTNSSYADLKGAVSLLAGNRVCAG
TDTLVSPGSKQVLKMLAREGLVEPLLDSGARLLECSCGPCIGMGGSPVSGGVSARTFNRNFEGRSGTKDGQVYLVSPLTA
AMAALRGCFTDPATWGEAPAVADLPAEVPSIADLFAYPPQDGAGTEVVYGPNIVALERFEPLPEQLQAGVLIRLGDDITT
DHIMPAGAEITSLRSNVPAIARHVFGRVDEDFVTRADALENGVIVAGENYGQGSSREHAALAPRHLGVRAVIAKSLARIH
RANLVNFGILPLILEDKADYAQLEQGCTVTLDTTLLAPGAVVQVTVDGGAAVAVRNDLTAKELDIIRAGGLLNYVRLSKT
AAA

Sequences:

>Translated_643_residues
MTMNLTQKIIAAHLVSGEMTQGAEIGLRIDQTLTQDATGTMAYLQFEAMGVDRVRTDLSVSYVDHNTLQMGFRNPDDHRF
LRTVAQKYGIVYSPAGTGICHQLHLENFAVPGSTLVGSDSHTPTAGGIGALAMGAGGLSVALAMAGEPYFITMPRVVKVR
LEGRLKGWATAKDVILHLLGLLTVKGGVGKVFEYAGPGVETLSVPERATITNMGAELGATTSVFPSDERTRDFLKAMGRA
DVWKPLAADDGAQYDEEVVIDLSALQPLAAQPHMPDRVVPVASLDGLKVDQVAIGSCTNSSYADLKGAVSLLAGNRVCAG
TDTLVSPGSKQVLKMLAREGLVEPLLDSGARLLECSCGPCIGMGGSPVSGGVSARTFNRNFEGRSGTKDGQVYLVSPLTA
AMAALRGCFTDPATWGEAPAVADLPAEVPSIADLFAYPPQDGAGTEVVYGPNIVALERFEPLPEQLQAGVLIRLGDDITT
DHIMPAGAEITSLRSNVPAIARHVFGRVDEDFVTRADALENGVIVAGENYGQGSSREHAALAPRHLGVRAVIAKSLARIH
RANLVNFGILPLILEDKADYAQLEQGCTVTLDTTLLAPGAVVQVTVDGGAAVAVRNDLTAKELDIIRAGGLLNYVRLSKT
AAA
>Mature_642_residues
TMNLTQKIIAAHLVSGEMTQGAEIGLRIDQTLTQDATGTMAYLQFEAMGVDRVRTDLSVSYVDHNTLQMGFRNPDDHRFL
RTVAQKYGIVYSPAGTGICHQLHLENFAVPGSTLVGSDSHTPTAGGIGALAMGAGGLSVALAMAGEPYFITMPRVVKVRL
EGRLKGWATAKDVILHLLGLLTVKGGVGKVFEYAGPGVETLSVPERATITNMGAELGATTSVFPSDERTRDFLKAMGRAD
VWKPLAADDGAQYDEEVVIDLSALQPLAAQPHMPDRVVPVASLDGLKVDQVAIGSCTNSSYADLKGAVSLLAGNRVCAGT
DTLVSPGSKQVLKMLAREGLVEPLLDSGARLLECSCGPCIGMGGSPVSGGVSARTFNRNFEGRSGTKDGQVYLVSPLTAA
MAALRGCFTDPATWGEAPAVADLPAEVPSIADLFAYPPQDGAGTEVVYGPNIVALERFEPLPEQLQAGVLIRLGDDITTD
HIMPAGAEITSLRSNVPAIARHVFGRVDEDFVTRADALENGVIVAGENYGQGSSREHAALAPRHLGVRAVIAKSLARIHR
ANLVNFGILPLILEDKADYAQLEQGCTVTLDTTLLAPGAVVQVTVDGGAAVAVRNDLTAKELDIIRAGGLLNYVRLSKTA
AA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=718, Percent_Identity=35.2367688022284, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI8659555, Length=832, Percent_Identity=27.8846153846154, Blast_Score=197, Evalue=4e-50,
Organism=Homo sapiens, GI41352693, Length=418, Percent_Identity=27.511961722488, Blast_Score=116, Evalue=6e-26,
Organism=Escherichia coli, GI87081781, Length=728, Percent_Identity=29.1208791208791, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1787531, Length=831, Percent_Identity=28.8808664259928, Blast_Score=196, Evalue=5e-51,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=29.9568965517241, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI2367097, Length=424, Percent_Identity=25.2358490566038, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=718, Percent_Identity=35.2367688022284, Blast_Score=325, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI32564738, Length=681, Percent_Identity=35.3891336270191, Blast_Score=315, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI25149342, Length=562, Percent_Identity=35.0533807829181, Blast_Score=262, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI17568399, Length=819, Percent_Identity=28.3272283272283, Blast_Score=200, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6322261, Length=736, Percent_Identity=33.1521739130435, Blast_Score=348, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6323335, Length=714, Percent_Identity=33.7535014005602, Blast_Score=330, Evalue=5e-91,
Organism=Saccharomyces cerevisiae, GI6320440, Length=620, Percent_Identity=28.5483870967742, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321429, Length=664, Percent_Identity=28.6144578313253, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI281365315, Length=718, Percent_Identity=34.8189415041783, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI17864292, Length=718, Percent_Identity=34.8189415041783, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI161076999, Length=682, Percent_Identity=35.4838709677419, Blast_Score=325, Evalue=7e-89,
Organism=Drosophila melanogaster, GI28571643, Length=716, Percent_Identity=33.9385474860335, Blast_Score=323, Evalue=3e-88,
Organism=Drosophila melanogaster, GI17137564, Length=824, Percent_Identity=27.7912621359223, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24645686, Length=823, Percent_Identity=27.2174969623329, Blast_Score=182, Evalue=9e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 67551; Mature: 67420

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMNLTQKIIAAHLVSGEMTQGAEIGLRIDQTLTQDATGTMAYLQFEAMGVDRVRTDLSV
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECHHHHCCCCCCEEEEEEEECCHHHHHCCCEE
SYVDHNTLQMGFRNPDDHRFLRTVAQKYGIVYSPAGTGICHQLHLENFAVPGSTLVGSDS
EEECCCEEEECCCCCCHHHHHHHHHHHCCEEECCCCCCCEEEEECCCCCCCCCEEECCCC
HTPTAGGIGALAMGAGGLSVALAMAGEPYFITMPRVVKVRLEGRLKGWATAKDVILHLLG
CCCCCCCCHHHHCCCCCCEEEEEECCCCEEEECCEEEEEEECCCCCCCCHHHHHHHHHHH
LLTVKGGVGKVFEYAGPGVETLSVPERATITNMGAELGATTSVFPSDERTRDFLKAMGRA
HHHCCCCCHHHHHHCCCCCEEECCCCCHHHHHCCHHHCCCCCCCCCCHHHHHHHHHCCCC
DVWKPLAADDGAQYDEEVVIDLSALQPLAAQPHMPDRVVPVASLDGLKVDQVAIGSCTNS
HHCCCCCCCCCCCCCCEEEEEHHHCCCHHCCCCCCCCEEEEECCCCCEECEEEECCCCCC
SYADLKGAVSLLAGNRVCAGTDTLVSPGSKQVLKMLAREGLVEPLLDSGARLLECSCGPC
CHHHHHHHHHHHCCCEEECCCCHHCCCCHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCE
IGMGGSPVSGGVSARTFNRNFEGRSGTKDGQVYLVSPLTAAMAALRGCFTDPATWGEAPA
ECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCCC
VADLPAEVPSIADLFAYPPQDGAGTEVVYGPNIVALERFEPLPEQLQAGVLIRLGDDITT
CCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCEEEECCCCCCCHHHCCCEEEEECCCCCC
DHIMPAGAEITSLRSNVPAIARHVFGRVDEDFVTRADALENGVIVAGENYGQGSSREHAA
CCCCCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCHH
LAPRHLGVRAVIAKSLARIHRANLVNFGILPLILEDKADYAQLEQGCTVTLDTTLLAPGA
CCCHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCEEEEEHEEECCCE
VVQVTVDGGAAVAVRNDLTAKELDIIRAGGLLNYVRLSKTAAA
EEEEEECCCEEEEEECCCCHHHHHEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TMNLTQKIIAAHLVSGEMTQGAEIGLRIDQTLTQDATGTMAYLQFEAMGVDRVRTDLSV
CCCHHHHHHHHHHHCCCCCCCCEEEEEECHHHHCCCCCCEEEEEEEECCHHHHHCCCEE
SYVDHNTLQMGFRNPDDHRFLRTVAQKYGIVYSPAGTGICHQLHLENFAVPGSTLVGSDS
EEECCCEEEECCCCCCHHHHHHHHHHHCCEEECCCCCCCEEEEECCCCCCCCCEEECCCC
HTPTAGGIGALAMGAGGLSVALAMAGEPYFITMPRVVKVRLEGRLKGWATAKDVILHLLG
CCCCCCCCHHHHCCCCCCEEEEEECCCCEEEECCEEEEEEECCCCCCCCHHHHHHHHHHH
LLTVKGGVGKVFEYAGPGVETLSVPERATITNMGAELGATTSVFPSDERTRDFLKAMGRA
HHHCCCCCHHHHHHCCCCCEEECCCCCHHHHHCCHHHCCCCCCCCCCHHHHHHHHHCCCC
DVWKPLAADDGAQYDEEVVIDLSALQPLAAQPHMPDRVVPVASLDGLKVDQVAIGSCTNS
HHCCCCCCCCCCCCCCEEEEEHHHCCCHHCCCCCCCCEEEEECCCCCEECEEEECCCCCC
SYADLKGAVSLLAGNRVCAGTDTLVSPGSKQVLKMLAREGLVEPLLDSGARLLECSCGPC
CHHHHHHHHHHHCCCEEECCCCHHCCCCHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCE
IGMGGSPVSGGVSARTFNRNFEGRSGTKDGQVYLVSPLTAAMAALRGCFTDPATWGEAPA
ECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCCC
VADLPAEVPSIADLFAYPPQDGAGTEVVYGPNIVALERFEPLPEQLQAGVLIRLGDDITT
CCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCEEEECCCCCCCHHHCCCEEEEECCCCCC
DHIMPAGAEITSLRSNVPAIARHVFGRVDEDFVTRADALENGVIVAGENYGQGSSREHAA
CCCCCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCHH
LAPRHLGVRAVIAKSLARIHRANLVNFGILPLILEDKADYAQLEQGCTVTLDTTLLAPGA
CCCHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCEEEEEHEEECCCE
VVQVTVDGGAAVAVRNDLTAKELDIIRAGGLLNYVRLSKTAAA
EEEEEECCCEEEEEECCCCHHHHHEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA