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The map label for this gene is flbD [H]

Identifier: 78356450

GI number: 78356450

Start: 1421151

End: 1422584

Strand: Direct

Name: flbD [H]

Synonym: Dde_1405

Alternate gene names: 78356450

Gene position: 1421151-1422584 (Clockwise)

Preceding gene: 78356445

Following gene: 78356451

Centisome position: 38.1

GC content: 58.3

Gene sequence:

>1434_bases
ATGTCATCAAGGCGCATTCTGTTTCTCGCACCGGCTCAGTCCGTCACGCGTATTTTCCCTCAGCTGAAAGAAGCCGGCTA
TGAAGTGGGGATTGCCGAAAACCTTAAAGGTGCATCGGCGTTTATCCGCAAATCAGGGCCGTCGGTTATTTTCTCGCGTC
CGCAGCTTCCGGGGTACAAGGTCGAAGACCTGCTTTCGGTCAGTGCTGACGATTCTTCATTTCCTCCCGTCATCATTTTC
ACCGACAAAGGCTCTGCAGAAGAAGCAGAGCGTTTTTTAGGCATGGGAGCACGCGATTACTGGCTTGAGCCGCTGCAGTT
TGAAAAGGTGAAAGCGGCTGTGCCCGCGGTGCCGCGGACACCGGCATCCGCACCGGCTTCCAGCACTCTGGGCGGGCGTA
CGCCCCTGCCCGAAGGTGACGGCTCGCGTATTATCGGCTCGCATCCTGCCGTGGCACGGGTGCTGGCATTGGCAAAGCAG
GTTGCGCCTTCCCGTGCCACGGTGCTGATCTCCGGCGAGTCAGGCACAGGCAAGGAAATGTTCGCGCGGTATCTGCACCG
CAGCAGCGACAGAGCAGACAAGGCCTTTGTGGCGGTCAACTGCGCTGCTTTGCCCGAGCACCTGCTTGAAAGTGAGCTTT
TCGGTCATGAAAAAGGATCGTTTACCGGAGCCATAGCCAGAAAGCTGGGAAAATTTGAACTGGCCAGCGGCGGTACGCTG
TTGCTGGATGAAATTTCGGAGATGGATCTGGGCCTGCAGGCCAAGCTGCTGCGCGTACTGCAGGAAGGCGAGATAGACCG
CGTGGGCGGGACCGAGACCATCAAGGTTGATGTGCGGGTGCTGGCCACCACCAACCGTTCGCTTGAAGACTGGGTCAAAG
AAGGGAAGTTCCGTCAGGATTTGTACTTCCGGCTCAATGTGATTCCGCTGCGCCTGCCTGCGCTGCGCGAACGCGGCGAT
GATATTGTGCGGCTGGCCCGTTTTTTCATAGCCATGTATGTGCGCGACTACGGCCTGCCTCCGCTGGAGCTGGCTCCTGC
CGCCGTGCAGTGGCTTGAGGAATATGACTGGCCGGGCAACGTGCGGGAGCTGCAGAACCTGATGGAGCGGGCATCGCTGC
TTGCGGGCAAAGGGCCCATCACTGTTTCTCATTTTCTGCTTGATCCCGACAGCTGGCCCGTGTTTGCCGATGAAGGCTCG
CATGAGCAGCAGCCGGATCAGGAGCATTGTGACGGTGCTTCGCCGGAAAGCGTCACCGATCCTTTCAGCGGTGGAGTCAT
TCCGCTGCATGAAATGGAGCGCATAATGATTCTCAAAGGTCTGGAGCAGACGTCCGGCAACCGGACCATGGCCGCAGACC
TGCTGGGGATTTCAGTGCGTACGCTGCGTAACAAGCTTAATGAATACCGCAGCGCCGGACTGGAAATACCCTGA

Upstream 100 bases:

>100_bases
TATGCCGCCTGTACGGGTATTTTGCGTATCTGTCTGAATTGTGGCATAATGATGCATTGGGGCATTGCAACCAGTTGTTC
TTACGGGTATTGAGATAAGC

Downstream 100 bases:

>100_bases
ACGGGACCGTATGACCGGTGGCCGGCGGTTCTGGCCTGCCACGGGCCGTTTTTTTCCGTTCCGGCGAAAAGAGCATGCCG
TCCTGCGGGGCGGTTTGCGC

Product: two component Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSSRRILFLAPAQSVTRIFPQLKEAGYEVGIAENLKGASAFIRKSGPSVIFSRPQLPGYKVEDLLSVSADDSSFPPVIIF
TDKGSAEEAERFLGMGARDYWLEPLQFEKVKAAVPAVPRTPASAPASSTLGGRTPLPEGDGSRIIGSHPAVARVLALAKQ
VAPSRATVLISGESGTGKEMFARYLHRSSDRADKAFVAVNCAALPEHLLESELFGHEKGSFTGAIARKLGKFELASGGTL
LLDEISEMDLGLQAKLLRVLQEGEIDRVGGTETIKVDVRVLATTNRSLEDWVKEGKFRQDLYFRLNVIPLRLPALRERGD
DIVRLARFFIAMYVRDYGLPPLELAPAAVQWLEEYDWPGNVRELQNLMERASLLAGKGPITVSHFLLDPDSWPVFADEGS
HEQQPDQEHCDGASPESVTDPFSGGVIPLHEMERIMILKGLEQTSGNRTMAADLLGISVRTLRNKLNEYRSAGLEIP

Sequences:

>Translated_477_residues
MSSRRILFLAPAQSVTRIFPQLKEAGYEVGIAENLKGASAFIRKSGPSVIFSRPQLPGYKVEDLLSVSADDSSFPPVIIF
TDKGSAEEAERFLGMGARDYWLEPLQFEKVKAAVPAVPRTPASAPASSTLGGRTPLPEGDGSRIIGSHPAVARVLALAKQ
VAPSRATVLISGESGTGKEMFARYLHRSSDRADKAFVAVNCAALPEHLLESELFGHEKGSFTGAIARKLGKFELASGGTL
LLDEISEMDLGLQAKLLRVLQEGEIDRVGGTETIKVDVRVLATTNRSLEDWVKEGKFRQDLYFRLNVIPLRLPALRERGD
DIVRLARFFIAMYVRDYGLPPLELAPAAVQWLEEYDWPGNVRELQNLMERASLLAGKGPITVSHFLLDPDSWPVFADEGS
HEQQPDQEHCDGASPESVTDPFSGGVIPLHEMERIMILKGLEQTSGNRTMAADLLGISVRTLRNKLNEYRSAGLEIP
>Mature_476_residues
SSRRILFLAPAQSVTRIFPQLKEAGYEVGIAENLKGASAFIRKSGPSVIFSRPQLPGYKVEDLLSVSADDSSFPPVIIFT
DKGSAEEAERFLGMGARDYWLEPLQFEKVKAAVPAVPRTPASAPASSTLGGRTPLPEGDGSRIIGSHPAVARVLALAKQV
APSRATVLISGESGTGKEMFARYLHRSSDRADKAFVAVNCAALPEHLLESELFGHEKGSFTGAIARKLGKFELASGGTLL
LDEISEMDLGLQAKLLRVLQEGEIDRVGGTETIKVDVRVLATTNRSLEDWVKEGKFRQDLYFRLNVIPLRLPALRERGDD
IVRLARFFIAMYVRDYGLPPLELAPAAVQWLEEYDWPGNVRELQNLMERASLLAGKGPITVSHFLLDPDSWPVFADEGSH
EQQPDQEHCDGASPESVTDPFSGGVIPLHEMERIMILKGLEQTSGNRTMAADLLGISVRTLRNKLNEYRSAGLEIP

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=39.957264957265, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1790437, Length=445, Percent_Identity=38.6516853932584, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=37.3931623931624, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789233, Length=328, Percent_Identity=42.3780487804878, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI87082152, Length=245, Percent_Identity=51.4285714285714, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=53.8793103448276, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1789087, Length=267, Percent_Identity=47.940074906367, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1788905, Length=306, Percent_Identity=40.5228758169935, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=351, Percent_Identity=39.3162393162393, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87081872, Length=334, Percent_Identity=37.4251497005988, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=38.6018237082067, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1789828, Length=250, Percent_Identity=36, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI87081858, Length=321, Percent_Identity=29.2834890965732, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52226; Mature: 52095

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRRILFLAPAQSVTRIFPQLKEAGYEVGIAENLKGASAFIRKSGPSVIFSRPQLPGYK
CCCCEEEEEECHHHHHHHHHHHHHCCCEECHHHCCCHHHHHHHCCCCCEEEECCCCCCCC
VEDLLSVSADDSSFPPVIIFTDKGSAEEAERFLGMGARDYWLEPLQFEKVKAAVPAVPRT
HHHHHEECCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHCCCHHHHHHHHHCCCCCCC
PASAPASSTLGGRTPLPEGDGSRIIGSHPAVARVLALAKQVAPSRATVLISGESGTGKEM
CCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHH
FARYLHRSSDRADKAFVAVNCAALPEHLLESELFGHEKGSFTGAIARKLGKFELASGGTL
HHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEECCCCEE
LLDEISEMDLGLQAKLLRVLQEGEIDRVGGTETIKVDVRVLATTNRSLEDWVKEGKFRQD
EEHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHCCCCCCC
LYFRLNVIPLRLPALRERGDDIVRLARFFIAMYVRDYGLPPLELAPAAVQWLEEYDWPGN
EEEEEEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
VRELQNLMERASLLAGKGPITVSHFLLDPDSWPVFADEGSHEQQPDQEHCDGASPESVTD
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEECCCCCCCCCCCHHHCCCCCCCCCCC
PFSGGVIPLHEMERIMILKGLEQTSGNRTMAADLLGISVRTLRNKLNEYRSAGLEIP
CCCCCEEEHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSRRILFLAPAQSVTRIFPQLKEAGYEVGIAENLKGASAFIRKSGPSVIFSRPQLPGYK
CCCEEEEEECHHHHHHHHHHHHHCCCEECHHHCCCHHHHHHHCCCCCEEEECCCCCCCC
VEDLLSVSADDSSFPPVIIFTDKGSAEEAERFLGMGARDYWLEPLQFEKVKAAVPAVPRT
HHHHHEECCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHCCCHHHHHHHHHCCCCCCC
PASAPASSTLGGRTPLPEGDGSRIIGSHPAVARVLALAKQVAPSRATVLISGESGTGKEM
CCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHH
FARYLHRSSDRADKAFVAVNCAALPEHLLESELFGHEKGSFTGAIARKLGKFELASGGTL
HHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEECCCCEE
LLDEISEMDLGLQAKLLRVLQEGEIDRVGGTETIKVDVRVLATTNRSLEDWVKEGKFRQD
EEHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHCCCCCCC
LYFRLNVIPLRLPALRERGDDIVRLARFFIAMYVRDYGLPPLELAPAAVQWLEEYDWPGN
EEEEEEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
VRELQNLMERASLLAGKGPITVSHFLLDPDSWPVFADEGSHEQQPDQEHCDGASPESVTD
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEECCCCCCCCCCCHHHCCCCCCCCCCC
PFSGGVIPLHEMERIMILKGLEQTSGNRTMAADLLGISVRTLRNKLNEYRSAGLEIP
CCCCCEEEHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]