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The map label for this gene is aegA [H]

Identifier: 78356297

GI number: 78356297

Start: 1263741

End: 1265171

Strand: Reverse

Name: aegA [H]

Synonym: Dde_1250

Alternate gene names: 78356297

Gene position: 1265171-1263741 (Counterclockwise)

Preceding gene: 78356298

Following gene: 78356295

Centisome position: 33.92

GC content: 59.89

Gene sequence:

>1431_bases
ATGAAAGCAAGTAAAAAGCCCGTCAGACCCAGAGTGCCCATGCCCGTGCAGCCGCCCGAAGAGCGGATACGCAATTTTGA
CGAAGTAGCAACGGGTTACACGCTGGAAATGGCCGCAGAAGAAGCCGCGCGCTGTCTGCAGTGCAAAAAACCAAAATGTG
TCAAAGGCTGTCCTGTGGAAGTGCCCATTCCGCAATTTATCGCGCATCTGGCGGCGGGCGACATAGACGCGGCCTACCGC
ACCCTGAAAAGCACCAACAGTCTGCCGGCAGTCTGCGGACGGGTGTGCCCGCAGGAGGTGCAATGCGAAGGCGAATGCAT
CCTGACAGCCAAAGGGCAGCCGGTGGCCATAGGCCGTCTGGAACGGTTTGTGGCAGACGAATACATGCACCGCGATGCCT
GCGGGCTTATTTCCGGCAAGCCGGAATGCCCGCTGATTGATCCGCACAAAAAGGTTGCCTGCATCGGCAGCGGACCGGCC
AGCCTGACAGCTGCCGGATATCTGGCGGCCAGAGGCTGCAAGGTGACCGTGTTTGAGGCTCTGCACGAACTGGGCGGGGT
GCTGGTCTACGGTATTCCGGAATTCCGACTGCCCAAAGAAAAAATAGTAGCCAAAGAAGTGAATGCCCTGCGCGAACTGG
AAGTGGACTTTGTCCGTAACCGTGTGGGCGGCAAGACATTCTCCATAGGCGAACTGTTCGCACAGGGGTACAGGGCGGTC
TTCATCGGCGTAGGAGCCGGTCTTCCCAGATTTCTGGGTATTCCCGGTGAAAATCTCAACGGGGTTTTTTCCGCCAATGA
ATACCTCACACGGGTCAATCTGGGACGGGCATACAGCTTTCCCGACTATGACACACCCATCATCCGCGGACGCCGTGTTA
CCGTTTTCGGGGGCGGCAACGTAGCCATGGACGCGGCCCGTACAGCTCTGCGGCTGGGTGCGGATTCTGTGCACATCGTC
TACAGGCGCACCGTGGCCGAAATGCCCGCCCGCCATGAAGAAGTGGAACACGCCATGGAAGAAGGCGTGAAAATGCAGGA
GCTGTGCGCTCCCATTGCGTTTGTGCCCGATGCGGAGGGCTGCCTGAGTGGTGTACGCCTGCAGCAGATGCAGCTGGGAG
AGCCTGATCAGTCCGGCAGGCGGCGTCCGGTGCCGGTGGAGGGAAGCGAACTGCTTCTGCCCACGGATCTGGCCGTCATT
GCGGTGGGAACCCGCTCCAATCCGGTACTGCTGGAGGCCGAACCGGAGCTTGACCTGAACAGATGGGGCTACATCAGCAT
AAACAGCGACACAGGCGAAACCAGCATACCCGACGTGTTTGCCGGCGGCGACATAGTCACCGGCGCAGCCACCGTGATAC
TGGCCATGGGCGCAGGACGCACAGCCGCCAAGGAAATTGCCCGCAGGCTCGGTTGTCAGCCCGACGACTAG

Upstream 100 bases:

>100_bases
GACGGCCATCAGGTGGACTTTGCCGAACTGCGGCAACGCCTGAACGCTTTCCGTCCGCTTGAGCGCGAATCCTATGACCA
TCACTGCAGGTGCAAATGCC

Downstream 100 bases:

>100_bases
CCCTCACGCCCCTGCCCGCTGCCGCCACGTCCAGCCCGGCGGCAGCGGGTCCGGTGCGGGGCAGACGGCAGCACCGGCGG
CTCCCTCTGGCACCGGCAGA

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKASKKPVRPRVPMPVQPPEERIRNFDEVATGYTLEMAAEEAARCLQCKKPKCVKGCPVEVPIPQFIAHLAAGDIDAAYR
TLKSTNSLPAVCGRVCPQEVQCEGECILTAKGQPVAIGRLERFVADEYMHRDACGLISGKPECPLIDPHKKVACIGSGPA
SLTAAGYLAARGCKVTVFEALHELGGVLVYGIPEFRLPKEKIVAKEVNALRELEVDFVRNRVGGKTFSIGELFAQGYRAV
FIGVGAGLPRFLGIPGENLNGVFSANEYLTRVNLGRAYSFPDYDTPIIRGRRVTVFGGGNVAMDAARTALRLGADSVHIV
YRRTVAEMPARHEEVEHAMEEGVKMQELCAPIAFVPDAEGCLSGVRLQQMQLGEPDQSGRRRPVPVEGSELLLPTDLAVI
AVGTRSNPVLLEAEPELDLNRWGYISINSDTGETSIPDVFAGGDIVTGAATVILAMGAGRTAAKEIARRLGCQPDD

Sequences:

>Translated_476_residues
MKASKKPVRPRVPMPVQPPEERIRNFDEVATGYTLEMAAEEAARCLQCKKPKCVKGCPVEVPIPQFIAHLAAGDIDAAYR
TLKSTNSLPAVCGRVCPQEVQCEGECILTAKGQPVAIGRLERFVADEYMHRDACGLISGKPECPLIDPHKKVACIGSGPA
SLTAAGYLAARGCKVTVFEALHELGGVLVYGIPEFRLPKEKIVAKEVNALRELEVDFVRNRVGGKTFSIGELFAQGYRAV
FIGVGAGLPRFLGIPGENLNGVFSANEYLTRVNLGRAYSFPDYDTPIIRGRRVTVFGGGNVAMDAARTALRLGADSVHIV
YRRTVAEMPARHEEVEHAMEEGVKMQELCAPIAFVPDAEGCLSGVRLQQMQLGEPDQSGRRRPVPVEGSELLLPTDLAVI
AVGTRSNPVLLEAEPELDLNRWGYISINSDTGETSIPDVFAGGDIVTGAATVILAMGAGRTAAKEIARRLGCQPDD
>Mature_476_residues
MKASKKPVRPRVPMPVQPPEERIRNFDEVATGYTLEMAAEEAARCLQCKKPKCVKGCPVEVPIPQFIAHLAAGDIDAAYR
TLKSTNSLPAVCGRVCPQEVQCEGECILTAKGQPVAIGRLERFVADEYMHRDACGLISGKPECPLIDPHKKVACIGSGPA
SLTAAGYLAARGCKVTVFEALHELGGVLVYGIPEFRLPKEKIVAKEVNALRELEVDFVRNRVGGKTFSIGELFAQGYRAV
FIGVGAGLPRFLGIPGENLNGVFSANEYLTRVNLGRAYSFPDYDTPIIRGRRVTVFGGGNVAMDAARTALRLGADSVHIV
YRRTVAEMPARHEEVEHAMEEGVKMQELCAPIAFVPDAEGCLSGVRLQQMQLGEPDQSGRRRPVPVEGSELLLPTDLAVI
AVGTRSNPVLLEAEPELDLNRWGYISINSDTGETSIPDVFAGGDIVTGAATVILAMGAGRTAAKEIARRLGCQPDD

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=476, Percent_Identity=31.7226890756302, Blast_Score=189, Evalue=4e-48,
Organism=Escherichia coli, GI1788811, Length=459, Percent_Identity=38.3442265795207, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI87082180, Length=477, Percent_Identity=37.1069182389937, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1789606, Length=466, Percent_Identity=36.2660944206009, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1788468, Length=445, Percent_Identity=32.3595505617978, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1789243, Length=465, Percent_Identity=33.9784946236559, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71984108, Length=470, Percent_Identity=32.1276595744681, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17570289, Length=480, Percent_Identity=28.3333333333333, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6320030, Length=481, Percent_Identity=30.7692307692308, Blast_Score=163, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24665547, Length=490, Percent_Identity=31.0204081632653, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24665543, Length=490, Percent_Identity=31.0204081632653, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=31.0204081632653, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=31.0204081632653, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24640763, Length=488, Percent_Identity=29.3032786885246, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18858217, Length=488, Percent_Identity=29.3032786885246, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 51243; Mature: 51243

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASKKPVRPRVPMPVQPPEERIRNFDEVATGYTLEMAAEEAARCLQCKKPKCVKGCPVE
CCCCCCCCCCCCCCCCCCHHHHHHCHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCCCCC
VPIPQFIAHLAAGDIDAAYRTLKSTNSLPAVCGRVCPQEVQCEGECILTAKGQPVAIGRL
CCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCCEEEEECCCCEEHHHH
ERFVADEYMHRDACGLISGKPECPLIDPHKKVACIGSGPASLTAAGYLAARGCKVTVFEA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEHHHHH
LHELGGVLVYGIPEFRLPKEKIVAKEVNALRELEVDFVRNRVGGKTFSIGELFAQGYRAV
HHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCEEE
FIGVGAGLPRFLGIPGENLNGVFSANEYLTRVNLGRAYSFPDYDTPIIRGRRVTVFGGGN
EEECCCCCHHHHCCCCCCCCCEEECCHHHHEEECCCEECCCCCCCCEECCCEEEEEECCC
VAMDAARTALRLGADSVHIVYRRTVAEMPARHEEVEHAMEEGVKMQELCAPIAFVPDAEG
EEHHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCEEECCCCHH
CLSGVRLQQMQLGEPDQSGRRRPVPVEGSELLLPTDLAVIAVGTRSNPVLLEAEPELDLN
HHHCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCCCCC
RWGYISINSDTGETSIPDVFAGGDIVTGAATVILAMGAGRTAAKEIARRLGCQPDD
CEEEEEEECCCCCCCCCCEECCCCCHHHHHHHHHEECCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKASKKPVRPRVPMPVQPPEERIRNFDEVATGYTLEMAAEEAARCLQCKKPKCVKGCPVE
CCCCCCCCCCCCCCCCCCHHHHHHCHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCCCCC
VPIPQFIAHLAAGDIDAAYRTLKSTNSLPAVCGRVCPQEVQCEGECILTAKGQPVAIGRL
CCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCCEEEEECCCCEEHHHH
ERFVADEYMHRDACGLISGKPECPLIDPHKKVACIGSGPASLTAAGYLAARGCKVTVFEA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEHHHHH
LHELGGVLVYGIPEFRLPKEKIVAKEVNALRELEVDFVRNRVGGKTFSIGELFAQGYRAV
HHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCEEE
FIGVGAGLPRFLGIPGENLNGVFSANEYLTRVNLGRAYSFPDYDTPIIRGRRVTVFGGGN
EEECCCCCHHHHCCCCCCCCCEEECCHHHHEEECCCEECCCCCCCCEECCCEEEEEECCC
VAMDAARTALRLGADSVHIVYRRTVAEMPARHEEVEHAMEEGVKMQELCAPIAFVPDAEG
EEHHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCEEECCCCHH
CLSGVRLQQMQLGEPDQSGRRRPVPVEGSELLLPTDLAVIAVGTRSNPVLLEAEPELDLN
HHHCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCCCCC
RWGYISINSDTGETSIPDVFAGGDIVTGAATVILAMGAGRTAAKEIARRLGCQPDD
CEEEEEEECCCCCCCCCCEECCCCCHHHHHHHHHEECCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]