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The map label for this gene is purH [H]

Identifier: 78355221

GI number: 78355221

Start: 158211

End: 159602

Strand: Reverse

Name: purH [H]

Synonym: Dde_0174

Alternate gene names: 78355221

Gene position: 159602-158211 (Counterclockwise)

Preceding gene: 78355222

Following gene: 78355220

Centisome position: 4.28

GC content: 60.99

Gene sequence:

>1392_bases
GTGGTACCCTGCCCGCCTCGCATGACCGCATGCGGCGGGGCGCCGGTTCACGCGCCGCCGCCATCCAATCAATCTGCACG
GGCGCGCGGCGTCCGTCTAGCCGGAGTTCACAGCAGCATGAGCGACCTCAAGAAAATGTACAGCACCCTGCAGGAAGATC
CGTTTCCTGCTGAAATGACCCTGCGTCTGGGCGATCAGGAACTGCATTACACCAAGCGCACCTGGACCATCGACGGTCAG
GAAAAAGGCCTGCGCTACGGCGAAAACCCCGACCAGCCCGCAGCCCTGTACCAGCTGGACAAGGGCAGCCTGACACTGGG
CGGGGTAACCTTCCGCGGGCCGGGTGCCGGTCTTGTCTCCTCCCTTGAGGAAAAGCACATGATTCAGGCCGGCAAGCACC
CCGGCAAGACCAATTTCACCGACGTGGACAACGCGCTGAATATCCTGCAATACCTTGATGCAAAGCCCGCTGCCCTCATC
ATGAAACATAACAACCCCAGCGGTGCAGCATGGAGCGGTGACGGCGTGGCAACCGCGCTGGAACGCGCCTTCTGGTGCGA
CCGCATCGCGGCTTTCGGCGGCGCGGTCATTGTCAACCGTCCGCTGGACAAAAAAGCAGCCGAGCTTATCAGTTCATGCT
ATTTCGAAGTGGTGGCGGCTCCTGATTTTGAAGAAGGCGTGGTTGAAATGCTCAAAAGCAGGAAAAATCTGCGTATTCTG
CAGATTCCCGGCATCAGCCGTCTGCCCGAACTGGCCCGCATGCCGTTCATCGACCTGAAGTCGCTGCAGGACGGCGGCAT
CATCATGCAGCACTCTTTCCGCAACCGCATTCTGAGCGCAGATGATTTTCTGCCTGCAACGGCGGAAAAAGACGGCGTCA
CCGTCAATGCGCGCAAACCTTCCCCGCAGGAAGCCGACGACCTGCTTTTCGCATGGGCTGTCGAAGCGGGCGTCACGTCC
AACTCTGTCATTTTTGCACGCAACGGCGCCACCGTATCCATCGGCACCGGCGAACAGGACCGCGTGGGCTGCGTGGAACT
GGCAGTGCACAAAGCCTATACCAAATATGCCGACACGCTGGCCTTCCGCGAACAGGGCTGTTCGCTGTACGAACTGAAGC
AGAAGGCAGCCGGCGATTCCGCAGCAGCCGAAGCTCTTGAAGACATCGAAGCCCGCACACGCGCCGACAGGGGCGGTCTG
CCCGGTACCGCCATGGTGTCCGACGGCTTTTTCCCCTTCCGCGACGGAGTGGATGCAGCCATTGCCCACGGGGTGGCCGC
CATTGCCCAGCCCGGCGGTTCCATACGCGACCACGAGGTCATCATGGCCTGCAACGAGGCTGCCCCGCAGGTAGCCATGG
TCTTCACCGGCCAGCGGTCTTTCAAGCATTAA

Upstream 100 bases:

>100_bases
GCTGCGGTCCGCACACGCCGGCCGGTAAAGGCCGGACGCGGTCCCACGCTACGCCAAACGGGGCGGGGCTTCAAATGGAA
ATTGCCCACCGCAACGCCCT

Downstream 100 bases:

>100_bases
CCGGCAGCGGAGGGCGGCATGCCGCCCTCCGCATACCGCTGAAACAAGCAACCACGCGCAAACACAAGCAGGAATCATGA
GCGCTACCTCCCCTCTGGTC

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MVPCPPRMTACGGAPVHAPPPSNQSARARGVRLAGVHSSMSDLKKMYSTLQEDPFPAEMTLRLGDQELHYTKRTWTIDGQ
EKGLRYGENPDQPAALYQLDKGSLTLGGVTFRGPGAGLVSSLEEKHMIQAGKHPGKTNFTDVDNALNILQYLDAKPAALI
MKHNNPSGAAWSGDGVATALERAFWCDRIAAFGGAVIVNRPLDKKAAELISSCYFEVVAAPDFEEGVVEMLKSRKNLRIL
QIPGISRLPELARMPFIDLKSLQDGGIIMQHSFRNRILSADDFLPATAEKDGVTVNARKPSPQEADDLLFAWAVEAGVTS
NSVIFARNGATVSIGTGEQDRVGCVELAVHKAYTKYADTLAFREQGCSLYELKQKAAGDSAAAEALEDIEARTRADRGGL
PGTAMVSDGFFPFRDGVDAAIAHGVAAIAQPGGSIRDHEVIMACNEAAPQVAMVFTGQRSFKH

Sequences:

>Translated_463_residues
MVPCPPRMTACGGAPVHAPPPSNQSARARGVRLAGVHSSMSDLKKMYSTLQEDPFPAEMTLRLGDQELHYTKRTWTIDGQ
EKGLRYGENPDQPAALYQLDKGSLTLGGVTFRGPGAGLVSSLEEKHMIQAGKHPGKTNFTDVDNALNILQYLDAKPAALI
MKHNNPSGAAWSGDGVATALERAFWCDRIAAFGGAVIVNRPLDKKAAELISSCYFEVVAAPDFEEGVVEMLKSRKNLRIL
QIPGISRLPELARMPFIDLKSLQDGGIIMQHSFRNRILSADDFLPATAEKDGVTVNARKPSPQEADDLLFAWAVEAGVTS
NSVIFARNGATVSIGTGEQDRVGCVELAVHKAYTKYADTLAFREQGCSLYELKQKAAGDSAAAEALEDIEARTRADRGGL
PGTAMVSDGFFPFRDGVDAAIAHGVAAIAQPGGSIRDHEVIMACNEAAPQVAMVFTGQRSFKH
>Mature_463_residues
MVPCPPRMTACGGAPVHAPPPSNQSARARGVRLAGVHSSMSDLKKMYSTLQEDPFPAEMTLRLGDQELHYTKRTWTIDGQ
EKGLRYGENPDQPAALYQLDKGSLTLGGVTFRGPGAGLVSSLEEKHMIQAGKHPGKTNFTDVDNALNILQYLDAKPAALI
MKHNNPSGAAWSGDGVATALERAFWCDRIAAFGGAVIVNRPLDKKAAELISSCYFEVVAAPDFEEGVVEMLKSRKNLRIL
QIPGISRLPELARMPFIDLKSLQDGGIIMQHSFRNRILSADDFLPATAEKDGVTVNARKPSPQEADDLLFAWAVEAGVTS
NSVIFARNGATVSIGTGEQDRVGCVELAVHKAYTKYADTLAFREQGCSLYELKQKAAGDSAAAEALEDIEARTRADRGGL
PGTAMVSDGFFPFRDGVDAAIAHGVAAIAQPGGSIRDHEVIMACNEAAPQVAMVFTGQRSFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=444, Percent_Identity=28.8288288288288, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI1790439, Length=397, Percent_Identity=32.9974811083123, Blast_Score=176, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71985556, Length=319, Percent_Identity=26.9592476489028, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985564, Length=434, Percent_Identity=26.4976958525346, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71985574, Length=371, Percent_Identity=24.2587601078167, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323056, Length=429, Percent_Identity=26.1072261072261, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323768, Length=413, Percent_Identity=27.3607748184019, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24649832, Length=436, Percent_Identity=27.7522935779816, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 49719; Mature: 49719

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPCPPRMTACGGAPVHAPPPSNQSARARGVRLAGVHSSMSDLKKMYSTLQEDPFPAEMT
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEE
LRLGDQELHYTKRTWTIDGQEKGLRYGENPDQPAALYQLDKGSLTLGGVTFRGPGAGLVS
EEECCCHHEEEEEEEEECCHHHCCCCCCCCCCCCEEEEECCCCEEECCEEEECCCCHHHH
SLEEKHMIQAGKHPGKTNFTDVDNALNILQYLDAKPAALIMKHNNPSGAAWSGDGVATAL
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHH
ERAFWCDRIAAFGGAVIVNRPLDKKAAELISSCYFEVVAAPDFEEGVVEMLKSRKNLRIL
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCEEEE
QIPGISRLPELARMPFIDLKSLQDGGIIMQHSFRNRILSADDFLPATAEKDGVTVNARKP
ECCCCHHCHHHHHCCCHHHCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCEEEECCCC
SPQEADDLLFAWAVEAGVTSNSVIFARNGATVSIGTGEQDRVGCVELAVHKAYTKYADTL
CCCCHHHEEEEEEHHCCCCCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
AFREQGCSLYELKQKAAGDSAAAEALEDIEARTRADRGGLPGTAMVSDGFFPFRDGVDAA
HHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHH
IAHGVAAIAQPGGSIRDHEVIMACNEAAPQVAMVFTGQRSFKH
HHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEECCHHCCC
>Mature Secondary Structure
MVPCPPRMTACGGAPVHAPPPSNQSARARGVRLAGVHSSMSDLKKMYSTLQEDPFPAEMT
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEE
LRLGDQELHYTKRTWTIDGQEKGLRYGENPDQPAALYQLDKGSLTLGGVTFRGPGAGLVS
EEECCCHHEEEEEEEEECCHHHCCCCCCCCCCCCEEEEECCCCEEECCEEEECCCCHHHH
SLEEKHMIQAGKHPGKTNFTDVDNALNILQYLDAKPAALIMKHNNPSGAAWSGDGVATAL
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHH
ERAFWCDRIAAFGGAVIVNRPLDKKAAELISSCYFEVVAAPDFEEGVVEMLKSRKNLRIL
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCEEEE
QIPGISRLPELARMPFIDLKSLQDGGIIMQHSFRNRILSADDFLPATAEKDGVTVNARKP
ECCCCHHCHHHHHCCCHHHCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCEEEECCCC
SPQEADDLLFAWAVEAGVTSNSVIFARNGATVSIGTGEQDRVGCVELAVHKAYTKYADTL
CCCCHHHEEEEEEHHCCCCCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
AFREQGCSLYELKQKAAGDSAAAEALEDIEARTRADRGGLPGTAMVSDGFFPFRDGVDAA
HHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHH
IAHGVAAIAQPGGSIRDHEVIMACNEAAPQVAMVFTGQRSFKH
HHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA