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The map label for this gene is mltF [H]

Identifier: 78355222

GI number: 78355222

Start: 160021

End: 161682

Strand: Reverse

Name: mltF [H]

Synonym: Dde_0175

Alternate gene names: 78355222

Gene position: 161682-160021 (Counterclockwise)

Preceding gene: 78355228

Following gene: 78355221

Centisome position: 4.33

GC content: 61.67

Gene sequence:

>1662_bases
ATGCAGGTCGCCTTTCCGACGCAAAACAGGAGTTCGGCGCACGGCATAAGGGATCATTACCTACATGCCGCCAATAGAAA
AGTCAAACGGATGGCCACCGGAAAAGGCCCCGCAACCTGCTTCGGACGGGCTCTCCTCTGTACGTTTCTCGCCTCGCATC
CCGTCCGGACACGTCACCTTCTCATGAAACAATCACATACACGCATGGGCAAGCGCCTGCTCGCCGCACTGTCAGTGACG
GTGCTGAGCATCCTGCTCTCATGCTCCGAGCGCGAAAAGCAGCCCGTGCAGCCCGACAACACGCTGCGCGTGGTGGCCCC
GCACAGGGAACGTGTTTCCAGCGCTCTGTCCCCTTACGGCCCGGGTTACGAACATGAACTTGTTATGCATTTTGCCCGCC
AGGCCGGCTATGATATTGAATGGATCGAAGCTTCCACCATGGAAGACGCCCTGCAGGCGCTGGCCGAAAGACGCGGCGAC
CTCATTGTGGGATACGGCGGCGACATAACCGCCGCTCCGCAGACAACAACCCGCCACAACGACGCACCGCCGCCGTCACC
CGTGGTGTCCGGCCCGGCGTACGGCCATTACAACCCGGTGCTGGTGCACAGCACGCGCCGCTACGGCCTGCGCCGCGGCA
ATGAAATGTGCGACACTCCGGTACTGGTCACCGCAGACCCCGAACTGGAAGAAATACTGGAAGCCAAGGCCGACACGCTG
GAATGCGTGCCCATGACCGAGGCTTCACAAGACGTCTACCTGACGCCGATTCTCCAGTCGCTCGACACCAACAGCGCCCG
GTTCGCCATGCTGGACAATCTGGGGTACAGCCTATGGCAGCCATTTTACCCCGGTGTGCGCACCGCCAAGGTGCTCACCC
AGAAAATACCATACCGCTGGTTCTGGCGCGGCGACGACGCGCGCCTCAACGAGGCTGCGCTGTCTTTCTGGGCCCAGCGC
GAAAACGACTCTCTGCTGGACGACATGCAGGAGCGGTATTTCGGCTTTCTGCCGGAAGAGGTGGACCGCTATGACGTGGC
ACTGCTGCGCCGCGCTCTGGAAAGCAAAGTACCGCAGTATGCACAGGCCATCGGCAACGCTGCCAGAGAACAGAGCATCG
ATCCGCTGCTGCTCATCGCCGTCATCTATCAGGAATCACGCTTTGACGCCGACGCACGCAGCAAAACAGGTGTGCGCGGC
CTGATGATGGTAACGCAGGACACCGCCCGGCTGCTGGGCATCAACCGCCTTGACCCTGAGCAGTCCATACGGGGCGGCGC
CCGCTACCTGCGCATGCTGTGGGATTCGCTGGAAGACATGGAGCTTGACCCCTGGAACCGCTGGTTTTTCACACTGGCGG
CATACAATCAGGGGCCCGGCCATCTGTTCGACGCCATCAAGCTCAGCCGCGCCCGCGGCGGCACCGGCCGCACGTGGCGC
GAACTGAAAGAGGTGTATCCTCTGCTTGCATGGGAACGCTGGTACAGCCGCACCAAGCACGGCTACTGTCGCGGGTTCGA
GGCGGTAGCCTATGTGGAGAACATCCGCTTCTACTATTACATCCTCAACGGATTACTCACTCTCGCGCGGCCTGAAACTG
AGGATCTTGGCCCTCTTCTGAGCGGGCTGCCCCCCGACTGGCCCGTCGGCGCCGGAACGTGA

Upstream 100 bases:

>100_bases
TCTATTTCCCGGGAAAGCAGGTCGACGATCTTACCTTTTCCTTCGTCGCCCCACTGTGCTCCCATGATTACCACGTTTGA
CATTTCCATCTCCTTGTGAC

Downstream 100 bases:

>100_bases
CGCTCCCTCGAACAGGCTGATATCGCCGTCGTAACCGGAAAGCGGCGGCAGTCCGTCATCGGGGCGGGCCATTTCGGACA
TATGGCTGTTATGCAGCTGG

Product: transglycosylase SLT domain-containing protein

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MQVAFPTQNRSSAHGIRDHYLHAANRKVKRMATGKGPATCFGRALLCTFLASHPVRTRHLLMKQSHTRMGKRLLAALSVT
VLSILLSCSEREKQPVQPDNTLRVVAPHRERVSSALSPYGPGYEHELVMHFARQAGYDIEWIEASTMEDALQALAERRGD
LIVGYGGDITAAPQTTTRHNDAPPPSPVVSGPAYGHYNPVLVHSTRRYGLRRGNEMCDTPVLVTADPELEEILEAKADTL
ECVPMTEASQDVYLTPILQSLDTNSARFAMLDNLGYSLWQPFYPGVRTAKVLTQKIPYRWFWRGDDARLNEAALSFWAQR
ENDSLLDDMQERYFGFLPEEVDRYDVALLRRALESKVPQYAQAIGNAAREQSIDPLLLIAVIYQESRFDADARSKTGVRG
LMMVTQDTARLLGINRLDPEQSIRGGARYLRMLWDSLEDMELDPWNRWFFTLAAYNQGPGHLFDAIKLSRARGGTGRTWR
ELKEVYPLLAWERWYSRTKHGYCRGFEAVAYVENIRFYYYILNGLLTLARPETEDLGPLLSGLPPDWPVGAGT

Sequences:

>Translated_553_residues
MQVAFPTQNRSSAHGIRDHYLHAANRKVKRMATGKGPATCFGRALLCTFLASHPVRTRHLLMKQSHTRMGKRLLAALSVT
VLSILLSCSEREKQPVQPDNTLRVVAPHRERVSSALSPYGPGYEHELVMHFARQAGYDIEWIEASTMEDALQALAERRGD
LIVGYGGDITAAPQTTTRHNDAPPPSPVVSGPAYGHYNPVLVHSTRRYGLRRGNEMCDTPVLVTADPELEEILEAKADTL
ECVPMTEASQDVYLTPILQSLDTNSARFAMLDNLGYSLWQPFYPGVRTAKVLTQKIPYRWFWRGDDARLNEAALSFWAQR
ENDSLLDDMQERYFGFLPEEVDRYDVALLRRALESKVPQYAQAIGNAAREQSIDPLLLIAVIYQESRFDADARSKTGVRG
LMMVTQDTARLLGINRLDPEQSIRGGARYLRMLWDSLEDMELDPWNRWFFTLAAYNQGPGHLFDAIKLSRARGGTGRTWR
ELKEVYPLLAWERWYSRTKHGYCRGFEAVAYVENIRFYYYILNGLLTLARPETEDLGPLLSGLPPDWPVGAGT
>Mature_553_residues
MQVAFPTQNRSSAHGIRDHYLHAANRKVKRMATGKGPATCFGRALLCTFLASHPVRTRHLLMKQSHTRMGKRLLAALSVT
VLSILLSCSEREKQPVQPDNTLRVVAPHRERVSSALSPYGPGYEHELVMHFARQAGYDIEWIEASTMEDALQALAERRGD
LIVGYGGDITAAPQTTTRHNDAPPPSPVVSGPAYGHYNPVLVHSTRRYGLRRGNEMCDTPVLVTADPELEEILEAKADTL
ECVPMTEASQDVYLTPILQSLDTNSARFAMLDNLGYSLWQPFYPGVRTAKVLTQKIPYRWFWRGDDARLNEAALSFWAQR
ENDSLLDDMQERYFGFLPEEVDRYDVALLRRALESKVPQYAQAIGNAAREQSIDPLLLIAVIYQESRFDADARSKTGVRG
LMMVTQDTARLLGINRLDPEQSIRGGARYLRMLWDSLEDMELDPWNRWFFTLAAYNQGPGHLFDAIKLSRARGGTGRTWR
ELKEVYPLLAWERWYSRTKHGYCRGFEAVAYVENIRFYYYILNGLLTLARPETEDLGPLLSGLPPDWPVGAGT

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=440, Percent_Identity=31.8181818181818, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 62578; Mature: 62578

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVAFPTQNRSSAHGIRDHYLHAANRKVKRMATGKGPATCFGRALLCTFLASHPVRTRHL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHH
LMKQSHTRMGKRLLAALSVTVLSILLSCSEREKQPVQPDNTLRVVAPHRERVSSALSPYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHCCCCC
PGYEHELVMHFARQAGYDIEWIEASTMEDALQALAERRGDLIVGYGGDITAAPQTTTRHN
CCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
DAPPPSPVVSGPAYGHYNPVLVHSTRRYGLRRGNEMCDTPVLVTADPELEEILEAKADTL
CCCCCCCCCCCCCCCCCCCEEEECHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHCCCCE
ECVPMTEASQDVYLTPILQSLDTNSARFAMLDNLGYSLWQPFYPGVRTAKVLTQKIPYRW
EEEECCCCCCCEEHHHHHHHCCCCCCCEEEHHHCCHHHHCCCCCCHHHHHHHHHHCCEEE
FWRGDDARLNEAALSFWAQRENDSLLDDMQERYFGFLPEEVDRYDVALLRRALESKVPQY
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AQAIGNAAREQSIDPLLLIAVIYQESRFDADARSKTGVRGLMMVTQDTARLLGINRLDPE
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEEHHHHHHHCCCCCCHH
QSIRGGARYLRMLWDSLEDMELDPWNRWFFTLAAYNQGPGHLFDAIKLSRARGGTGRTWR
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHH
ELKEVYPLLAWERWYSRTKHGYCRGFEAVAYVENIRFYYYILNGLLTLARPETEDLGPLL
HHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SGLPPDWPVGAGT
HCCCCCCCCCCCC
>Mature Secondary Structure
MQVAFPTQNRSSAHGIRDHYLHAANRKVKRMATGKGPATCFGRALLCTFLASHPVRTRHL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHH
LMKQSHTRMGKRLLAALSVTVLSILLSCSEREKQPVQPDNTLRVVAPHRERVSSALSPYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHCCCCC
PGYEHELVMHFARQAGYDIEWIEASTMEDALQALAERRGDLIVGYGGDITAAPQTTTRHN
CCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
DAPPPSPVVSGPAYGHYNPVLVHSTRRYGLRRGNEMCDTPVLVTADPELEEILEAKADTL
CCCCCCCCCCCCCCCCCCCEEEECHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHCCCCE
ECVPMTEASQDVYLTPILQSLDTNSARFAMLDNLGYSLWQPFYPGVRTAKVLTQKIPYRW
EEEECCCCCCCEEHHHHHHHCCCCCCCEEEHHHCCHHHHCCCCCCHHHHHHHHHHCCEEE
FWRGDDARLNEAALSFWAQRENDSLLDDMQERYFGFLPEEVDRYDVALLRRALESKVPQY
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AQAIGNAAREQSIDPLLLIAVIYQESRFDADARSKTGVRGLMMVTQDTARLLGINRLDPE
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEEHHHHHHHCCCCCCHH
QSIRGGARYLRMLWDSLEDMELDPWNRWFFTLAAYNQGPGHLFDAIKLSRARGGTGRTWR
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHH
ELKEVYPLLAWERWYSRTKHGYCRGFEAVAYVENIRFYYYILNGLLTLARPETEDLGPLL
HHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SGLPPDWPVGAGT
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA