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The map label for this gene is trmE

Identifier: 78224743

GI number: 78224743

Start: 3993637

End: 3995010

Strand: Reverse

Name: trmE

Synonym: Gmet_3560

Alternate gene names: 78224743

Gene position: 3995010-3993637 (Counterclockwise)

Preceding gene: 78224744

Following gene: 78224742

Centisome position: 99.94

GC content: 63.46

Gene sequence:

>1374_bases
ATGTACCTGGAAGATACCATAGCGGCCATCGGCACGCCGGTGGGGGAGGGGGGGATCGGCATCATCCGCGTGAGCGGTCC
CGATGTCCCCGCCATTGCCCGGCGGATCGTCCGGCGGGTTAACGAGAACGGTGACTTCGTGAGTCACCGTTTTTATTATG
GCACCGTAGTGGATCCCGAGTCGCGGGACACCGTTGACGAGGTTATGGCGGTGCTGATGCGGGCACCCCGTTCCTTCACC
CGGGAGGATGTCCTTGAGATCCAGTGCCACGGGGGATACCTGGTCACCCGCCGGGTCCTGGACGCGGTGCTCCAGTGCGG
CGCCCGTCCGGCCGAGCCGGGGGAGTTTACCCGCCGGGCGTTCCTGAACGGCCGCATTGATCTCGTTCAGGCCGAGGCGG
TCATCGACGTGATCCGCTCCAAGACCGAGGCGGCTCTCAATCTGGCCCAGCACCAGCGGGAGGGGCGCCTCTCCGAGCGG
CTCAAGGCCGTGCAGGGTTGCCTGCGACATAGTCTTGCCCTTGTTGAGGCGTTCATCGATTTCCCCGATGACGAGGTGGA
TCCCGCCTCCCGGGTGGAGATCGAGGCAAAGGCCCGGGAGGCCTCGGGCCGAATTGAAGAACTCCTGGAGGGTTTCGACG
AGGGGAGGGTGCTCCGGGACGGGGTGTCGGTCCTCATTGCCGGGAAGCCCAACGTGGGGAAGTCGAGTCTCCTTAATACG
CTCCTCCAGGAGAAGCGCGCCATCGTCACGTCGGTCCCCGGCACCACCCGGGACATCATTGAGGAGGTGGTTAACGTCCG
GGGCCTGCCGCTCCGGATGCTGGACACCGCCGGCATTCGGGAGACTGAGGATGTCGTCGAGCAGGAGGGGGTCCGTCTCA
CCCTGGAGAAGATACCCCAGGCTGACCTGATCCTTTTCGTCCTCGACGGTTCCCGTCCCTTTGACGACGACGACCGGATG
ATTCTTGCCGCCCTGGCGGAACGGCGCGTAATTGTCGTCACGAACAAGAGCGATCTTCCGGTCACCCTTCGTATTCCCGG
CGAGCTGGAGGGTGTGCACACCGTTGCTATTTCCACTGCCACCGGGGCCGGCATCGACGACCTGCGGGAGGCCGTTTTCG
AAACCTTCATTCACGGTCGAGCCATTGACAGCCGGGAGTATGTGGCCCTCTCGCAGACACGGCACCGGGATGCCCTGGTA
AAGGCTCGGGGAAGAATCGCCGTATTCTTCGCCAATCTGGCGGCGGGCAACGATCTGGAAATTCTGGCCGTGGACCTCAG
GGACGCCCTTGATGCCGTTGGGGAGGTTACCGGCGAAACGACCCCCGATGATATTCTTGAGCTTATCTTCCAGCGGTTCT
GCATCGGGAAGTAG

Upstream 100 bases:

>100_bases
GGACTCGTCCTCTACTGGCTCGTGAACAACGTTCTGACCATTATCCAGCAATCCTACATCAACAAGAGCATCGCCGCGGC
CGAGGCCAAGTAGGGACACG

Downstream 100 bases:

>100_bases
GTTTGACGAACAAGGGGTGCAACGGGTATCATTGGGTAGATGTTTCACGTGGAACATTGTGGCTAACTTCTTGTTGCCAC
TGACTAATTTGCTGGGATAC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MYLEDTIAAIGTPVGEGGIGIIRVSGPDVPAIARRIVRRVNENGDFVSHRFYYGTVVDPESRDTVDEVMAVLMRAPRSFT
REDVLEIQCHGGYLVTRRVLDAVLQCGARPAEPGEFTRRAFLNGRIDLVQAEAVIDVIRSKTEAALNLAQHQREGRLSER
LKAVQGCLRHSLALVEAFIDFPDDEVDPASRVEIEAKAREASGRIEELLEGFDEGRVLRDGVSVLIAGKPNVGKSSLLNT
LLQEKRAIVTSVPGTTRDIIEEVVNVRGLPLRMLDTAGIRETEDVVEQEGVRLTLEKIPQADLILFVLDGSRPFDDDDRM
ILAALAERRVIVVTNKSDLPVTLRIPGELEGVHTVAISTATGAGIDDLREAVFETFIHGRAIDSREYVALSQTRHRDALV
KARGRIAVFFANLAAGNDLEILAVDLRDALDAVGEVTGETTPDDILELIFQRFCIGK

Sequences:

>Translated_457_residues
MYLEDTIAAIGTPVGEGGIGIIRVSGPDVPAIARRIVRRVNENGDFVSHRFYYGTVVDPESRDTVDEVMAVLMRAPRSFT
REDVLEIQCHGGYLVTRRVLDAVLQCGARPAEPGEFTRRAFLNGRIDLVQAEAVIDVIRSKTEAALNLAQHQREGRLSER
LKAVQGCLRHSLALVEAFIDFPDDEVDPASRVEIEAKAREASGRIEELLEGFDEGRVLRDGVSVLIAGKPNVGKSSLLNT
LLQEKRAIVTSVPGTTRDIIEEVVNVRGLPLRMLDTAGIRETEDVVEQEGVRLTLEKIPQADLILFVLDGSRPFDDDDRM
ILAALAERRVIVVTNKSDLPVTLRIPGELEGVHTVAISTATGAGIDDLREAVFETFIHGRAIDSREYVALSQTRHRDALV
KARGRIAVFFANLAAGNDLEILAVDLRDALDAVGEVTGETTPDDILELIFQRFCIGK
>Mature_457_residues
MYLEDTIAAIGTPVGEGGIGIIRVSGPDVPAIARRIVRRVNENGDFVSHRFYYGTVVDPESRDTVDEVMAVLMRAPRSFT
REDVLEIQCHGGYLVTRRVLDAVLQCGARPAEPGEFTRRAFLNGRIDLVQAEAVIDVIRSKTEAALNLAQHQREGRLSER
LKAVQGCLRHSLALVEAFIDFPDDEVDPASRVEIEAKAREASGRIEELLEGFDEGRVLRDGVSVLIAGKPNVGKSSLLNT
LLQEKRAIVTSVPGTTRDIIEEVVNVRGLPLRMLDTAGIRETEDVVEQEGVRLTLEKIPQADLILFVLDGSRPFDDDDRM
ILAALAERRVIVVTNKSDLPVTLRIPGELEGVHTVAISTATGAGIDDLREAVFETFIHGRAIDSREYVALSQTRHRDALV
KARGRIAVFFANLAAGNDLEILAVDLRDALDAVGEVTGETTPDDILELIFQRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=34.6153846153846, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=32.4, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=33.6980306345733, Blast_Score=209, Evalue=5e-54,
Organism=Escherichia coli, GI2367268, Length=466, Percent_Identity=37.5536480686695, Blast_Score=267, Evalue=9e-73,
Organism=Escherichia coli, GI87082120, Length=216, Percent_Identity=30.0925925925926, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=461, Percent_Identity=31.8872017353579, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=509, Percent_Identity=31.041257367387, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI45550813, Length=477, Percent_Identity=33.7526205450734, Blast_Score=215, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_GEOMG (Q39PQ9)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386490.1
- HSSP:   Q9WYA4
- ProteinModelPortal:   Q39PQ9
- SMR:   Q39PQ9
- STRING:   Q39PQ9
- GeneID:   3739859
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3560
- NMPDR:   fig|269799.3.peg.3528
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q39PQ9
- ProtClustDB:   PRK05291
- BioCyc:   GMET269799:GMET_3560-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50168; Mature: 50168

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 86-86 BINDING 125-125 BINDING 457-457

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLEDTIAAIGTPVGEGGIGIIRVSGPDVPAIARRIVRRVNENGDFVSHRFYYGTVVDPE
CCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCC
SRDTVDEVMAVLMRAPRSFTREDVLEIQCHGGYLVTRRVLDAVLQCGARPAEPGEFTRRA
CCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
FLNGRIDLVQAEAVIDVIRSKTEAALNLAQHQREGRLSERLKAVQGCLRHSLALVEAFID
HHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDDEVDPASRVEIEAKAREASGRIEELLEGFDEGRVLRDGVSVLIAGKPNVGKSSLLNT
CCCCCCCCCHHEEEEHHHHHHCCHHHHHHHCCCCCCEEECCCEEEEECCCCCCHHHHHHH
LLQEKRAIVTSVPGTTRDIIEEVVNVRGLPLRMLDTAGIRETEDVVEQEGVRLTLEKIPQ
HHHHHHHEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHCCCEEEHHHCCC
ADLILFVLDGSRPFDDDDRMILAALAERRVIVVTNKSDLPVTLRIPGELEGVHTVAISTA
CCEEEEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCEEEEEEECC
TGAGIDDLREAVFETFIHGRAIDSREYVALSQTRHRDALVKARGRIAVFFANLAAGNDLE
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCE
ILAVDLRDALDAVGEVTGETTPDDILELIFQRFCIGK
EEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYLEDTIAAIGTPVGEGGIGIIRVSGPDVPAIARRIVRRVNENGDFVSHRFYYGTVVDPE
CCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCC
SRDTVDEVMAVLMRAPRSFTREDVLEIQCHGGYLVTRRVLDAVLQCGARPAEPGEFTRRA
CCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
FLNGRIDLVQAEAVIDVIRSKTEAALNLAQHQREGRLSERLKAVQGCLRHSLALVEAFID
HHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDDEVDPASRVEIEAKAREASGRIEELLEGFDEGRVLRDGVSVLIAGKPNVGKSSLLNT
CCCCCCCCCHHEEEEHHHHHHCCHHHHHHHCCCCCCEEECCCEEEEECCCCCCHHHHHHH
LLQEKRAIVTSVPGTTRDIIEEVVNVRGLPLRMLDTAGIRETEDVVEQEGVRLTLEKIPQ
HHHHHHHEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHCCCEEEHHHCCC
ADLILFVLDGSRPFDDDDRMILAALAERRVIVVTNKSDLPVTLRIPGELEGVHTVAISTA
CCEEEEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCEEEEEEECC
TGAGIDDLREAVFETFIHGRAIDSREYVALSQTRHRDALVKARGRIAVFFANLAAGNDLE
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCE
ILAVDLRDALDAVGEVTGETTPDDILELIFQRFCIGK
EEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA