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The map label for this gene is gidA

Identifier: 78224742

GI number: 78224742

Start: 3991612

End: 3993486

Strand: Reverse

Name: gidA

Synonym: Gmet_3559

Alternate gene names: 78224742

Gene position: 3993486-3991612 (Counterclockwise)

Preceding gene: 78224743

Following gene: 78224741

Centisome position: 99.9

GC content: 66.13

Gene sequence:

>1875_bases
ATGGAATTCATCGACTACGATAAACAGTACGACGTCATCGTGGTGGGGGCGGGCCATGCCGGCTGCGAGGCGGCCCTGGC
CGCTGCCCGCATGGGGTGCGAGACGCTGCTTCTCACCATTAATCTGGACGCCATTGCCCTCATGTCGTGCAACCCTGCCA
TCGGCGGGTCGGCCAAGGGGCATCTGGTGAAGGAGATCGATGCCCTCGGCGGCGAGATGGGAAAGAATATCGACGCCACC
GGCATCCAGTTCCGCATCCTCAATACCCGCAAGGGGCCGGCGGTCCGGGCCTCCCGGGCCCAGGCCGACAAGCAGCTCTA
CCGTCTCCGGATGAAACACGTCATGGAGCAGCAGGAACGCCTCTCCCTCAAGCAGGCCGAGGTGACCGGCATTGTGGTTG
AGGATGGCGAGGTACGCGGTGTCGATACGAAGGTCGGGGTCCGCTACCTCGGCGCCACCGTCATCCTCACCACCGGCACC
TTCATGCGGGGGCTCATCCATGTGGGGCTCACCAACTACCCCGGGGGGAGGGCGGGGGATCTACCGTCCGTGGGGCTGTC
GGACCAGCTCCGGGAGCTGGGCTTTGCCGTGGGCCGTCTCAAGACGGGCACCCCGGCCCGGCTCGACGGCCGCACCATTG
ATTTCACCCGCCTGGAGCCCCAGCACGGGGATGACCCGCCAGTACCGTTCTCTTTCTCCACGGAGCGGATCGACCAGCCC
CAGGTGCCGTGCTACATCGCCTACACCAACCCCCGCTCCCACGAGATCATCCGCTCGGGGCTCGACCGCTCGCCCCTCTA
TGCCGGCGTGATCGAAGGAATCGGTCCCCGCTATTGCCCCTCCATCGAGGACAAGGTGGTCCGCTTCCCCGAGAAGGACC
GTCACCAGACCTTTCTGGAGCCCGAGGGGCGGGACACGGTGGAGTACTATCCCTCGGGGCTCTCCACCTCGCTCCCCATC
GATATCCAGTGGGCCTTCTACCGGAGCATCGAGGGGCTGGAGCGGGTGGAGATCATGCGGCCCGCCTATGCCATCGAGTA
CGATTACGTGGACCCGATCCAGCTCCACGCCTCCCTGGAGACCAAGCTTGTCCGTAACCTCTACCACGCCGGCCAGATTA
ACGGCACCTCCGGCTACGAAGAGGCGGCGGGGCAGGGGCTCATGGCGGGGATCAACGCCGCCCTGCGGGTGCGGGACAAA
GAACCGCTGATTCTGGGCCGGAGCGAGGCTTATATCGGCGTCATGATCGACGACCTTGTTACCCTCGGCACCCGGGAACC
CTACCGGATGTTCACCTCACGGGCCGAGTACCGGCTCCTCCTGCGGGAGGACAACGCGGACCTGCGGCTCCGGGAGCGGG
GCCACGCCGTGGGGCTCGTGCCGGAAGGAGCGTACCAACGGTTCCTGGAGAAACGGGAGCGGATCGGTGCCGAGCTTGCT
CGGCTCAAGGGAACGAAACTCCTCCCCTCCGAAGCGGATCCGGATTTCCTGGCCGCGTTCTCCCTGACAGCTCTCCAGAA
TGCCCTCACCTATGAGCAGCTCCTGCGGCGTCCCGATATAACCTACGAGGAATTATGCCGCATCGATCCCCGGGCGGCGG
AGGTGCCGCCGGTGGTTCGGGAGCAGGTGGAGATCCAGATCAAGTACCAGGGGTACATCGAGCGGCAGCTGGACCAGGTG
GAGCGGGCCCGGAAGCTGGAAGGGACCCGTGTTCCCGCCGGCTTCGACTACGGGGCCCTCCCCGGCCTCTCGGCCGAGGT
GCGGGAGAAGCTGGCCAAATTCCGTCCCGACACCCTGGGGCAGGCCTCCCGCATCCAGGGGGTAACCCCGGCGGCGGTGG
GGATTCTGTCCCTTGCCCTCAAGGCCCGGGGGTAG

Upstream 100 bases:

>100_bases
GGAACATTGTGGCTAACTTCTTGTTGCCACTGACTAATTTGCTGGGATACCGTATCCATTATCGCCTCCTGTGAAACAGG
ATTGAGGTCATACGCAAGAG

Downstream 100 bases:

>100_bases
CATGGACCAGGAAGCCAAGGACCTCCTGGTGCGCGGAGCGGCCCAGCTGGGTGTGGAACTCACGTCGGCCCAAGTGGGGA
AATTTGCTCTCTTTGCCGCT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MEFIDYDKQYDVIVVGAGHAGCEAALAAARMGCETLLLTINLDAIALMSCNPAIGGSAKGHLVKEIDALGGEMGKNIDAT
GIQFRILNTRKGPAVRASRAQADKQLYRLRMKHVMEQQERLSLKQAEVTGIVVEDGEVRGVDTKVGVRYLGATVILTTGT
FMRGLIHVGLTNYPGGRAGDLPSVGLSDQLRELGFAVGRLKTGTPARLDGRTIDFTRLEPQHGDDPPVPFSFSTERIDQP
QVPCYIAYTNPRSHEIIRSGLDRSPLYAGVIEGIGPRYCPSIEDKVVRFPEKDRHQTFLEPEGRDTVEYYPSGLSTSLPI
DIQWAFYRSIEGLERVEIMRPAYAIEYDYVDPIQLHASLETKLVRNLYHAGQINGTSGYEEAAGQGLMAGINAALRVRDK
EPLILGRSEAYIGVMIDDLVTLGTREPYRMFTSRAEYRLLLREDNADLRLRERGHAVGLVPEGAYQRFLEKRERIGAELA
RLKGTKLLPSEADPDFLAAFSLTALQNALTYEQLLRRPDITYEELCRIDPRAAEVPPVVREQVEIQIKYQGYIERQLDQV
ERARKLEGTRVPAGFDYGALPGLSAEVREKLAKFRPDTLGQASRIQGVTPAAVGILSLALKARG

Sequences:

>Translated_624_residues
MEFIDYDKQYDVIVVGAGHAGCEAALAAARMGCETLLLTINLDAIALMSCNPAIGGSAKGHLVKEIDALGGEMGKNIDAT
GIQFRILNTRKGPAVRASRAQADKQLYRLRMKHVMEQQERLSLKQAEVTGIVVEDGEVRGVDTKVGVRYLGATVILTTGT
FMRGLIHVGLTNYPGGRAGDLPSVGLSDQLRELGFAVGRLKTGTPARLDGRTIDFTRLEPQHGDDPPVPFSFSTERIDQP
QVPCYIAYTNPRSHEIIRSGLDRSPLYAGVIEGIGPRYCPSIEDKVVRFPEKDRHQTFLEPEGRDTVEYYPSGLSTSLPI
DIQWAFYRSIEGLERVEIMRPAYAIEYDYVDPIQLHASLETKLVRNLYHAGQINGTSGYEEAAGQGLMAGINAALRVRDK
EPLILGRSEAYIGVMIDDLVTLGTREPYRMFTSRAEYRLLLREDNADLRLRERGHAVGLVPEGAYQRFLEKRERIGAELA
RLKGTKLLPSEADPDFLAAFSLTALQNALTYEQLLRRPDITYEELCRIDPRAAEVPPVVREQVEIQIKYQGYIERQLDQV
ERARKLEGTRVPAGFDYGALPGLSAEVREKLAKFRPDTLGQASRIQGVTPAAVGILSLALKARG
>Mature_624_residues
MEFIDYDKQYDVIVVGAGHAGCEAALAAARMGCETLLLTINLDAIALMSCNPAIGGSAKGHLVKEIDALGGEMGKNIDAT
GIQFRILNTRKGPAVRASRAQADKQLYRLRMKHVMEQQERLSLKQAEVTGIVVEDGEVRGVDTKVGVRYLGATVILTTGT
FMRGLIHVGLTNYPGGRAGDLPSVGLSDQLRELGFAVGRLKTGTPARLDGRTIDFTRLEPQHGDDPPVPFSFSTERIDQP
QVPCYIAYTNPRSHEIIRSGLDRSPLYAGVIEGIGPRYCPSIEDKVVRFPEKDRHQTFLEPEGRDTVEYYPSGLSTSLPI
DIQWAFYRSIEGLERVEIMRPAYAIEYDYVDPIQLHASLETKLVRNLYHAGQINGTSGYEEAAGQGLMAGINAALRVRDK
EPLILGRSEAYIGVMIDDLVTLGTREPYRMFTSRAEYRLLLREDNADLRLRERGHAVGLVPEGAYQRFLEKRERIGAELA
RLKGTKLLPSEADPDFLAAFSLTALQNALTYEQLLRRPDITYEELCRIDPRAAEVPPVVREQVEIQIKYQGYIERQLDQV
ERARKLEGTRVPAGFDYGALPGLSAEVREKLAKFRPDTLGQASRIQGVTPAAVGILSLALKARG

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=623, Percent_Identity=44.7833065810594, Blast_Score=494, Evalue=1e-140,
Organism=Homo sapiens, GI19882217, Length=648, Percent_Identity=43.0555555555556, Blast_Score=481, Evalue=1e-135,
Organism=Homo sapiens, GI183227703, Length=663, Percent_Identity=42.0814479638009, Blast_Score=476, Evalue=1e-134,
Organism=Escherichia coli, GI2367273, Length=621, Percent_Identity=57.0048309178744, Blast_Score=689, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=639, Percent_Identity=40.2190923317684, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6321202, Length=619, Percent_Identity=44.5880452342488, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24658174, Length=634, Percent_Identity=43.6908517350158, Blast_Score=471, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_GEOMG (Q39PR0)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386489.1
- ProteinModelPortal:   Q39PR0
- SMR:   Q39PR0
- STRING:   Q39PR0
- GeneID:   3739858
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3559
- NMPDR:   fig|269799.3.peg.3527
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   Q39PR0
- ProtClustDB:   PRK05192
- BioCyc:   GMET269799:GMET_3559-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69063; Mature: 69063

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 128-128 BINDING 183-183 BINDING 372-372

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFIDYDKQYDVIVVGAGHAGCEAALAAARMGCETLLLTINLDAIALMSCNPAIGGSAKG
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCEEEEEECCCCCCCCCCC
HLVKEIDALGGEMGKNIDATGIQFRILNTRKGPAVRASRAQADKQLYRLRMKHVMEQQER
HHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSLKQAEVTGIVVEDGEVRGVDTKVGVRYLGATVILTTGTFMRGLIHVGLTNYPGGRAGD
HHHHHEEEEEEEEECCCEECCCHHHHHHHHCEEEEEECCHHHHHHHHHCCCCCCCCCCCC
LPSVGLSDQLRELGFAVGRLKTGTPARLDGRTIDFTRLEPQHGDDPPVPFSFSTERIDQP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCC
QVPCYIAYTNPRSHEIIRSGLDRSPLYAGVIEGIGPRYCPSIEDKVVRFPEKDRHQTFLE
CCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCC
PEGRDTVEYYPSGLSTSLPIDIQWAFYRSIEGLERVEIMRPAYAIEYDYVDPIQLHASLE
CCCCCCHHCCCCCCCCCCCEEEEHHHHHHHCHHHHHHHHCCCEEEEECCCCHHHHHHHHH
TKLVRNLYHAGQINGTSGYEEAAGQGLMAGINAALRVRDKEPLILGRSEAYIGVMIDDLV
HHHHHHHHHCCCCCCCCCHHHHHCCCHHHCCCEEEEEECCCCEEEECCCCEEEEEHHHHH
TLGTREPYRMFTSRAEYRLLLREDNADLRLRERGHAVGLVPEGAYQRFLEKRERIGAELA
HHCCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHH
RLKGTKLLPSEADPDFLAAFSLTALQNALTYEQLLRRPDITYEELCRIDPRAAEVPPVVR
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHC
EQVEIQIKYQGYIERQLDQVERARKLEGTRVPAGFDYGALPGLSAEVREKLAKFRPDTLG
CCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
QASRIQGVTPAAVGILSLALKARG
CHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFIDYDKQYDVIVVGAGHAGCEAALAAARMGCETLLLTINLDAIALMSCNPAIGGSAKG
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCEEEEEECCCCCCCCCCC
HLVKEIDALGGEMGKNIDATGIQFRILNTRKGPAVRASRAQADKQLYRLRMKHVMEQQER
HHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSLKQAEVTGIVVEDGEVRGVDTKVGVRYLGATVILTTGTFMRGLIHVGLTNYPGGRAGD
HHHHHEEEEEEEEECCCEECCCHHHHHHHHCEEEEEECCHHHHHHHHHCCCCCCCCCCCC
LPSVGLSDQLRELGFAVGRLKTGTPARLDGRTIDFTRLEPQHGDDPPVPFSFSTERIDQP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCC
QVPCYIAYTNPRSHEIIRSGLDRSPLYAGVIEGIGPRYCPSIEDKVVRFPEKDRHQTFLE
CCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCC
PEGRDTVEYYPSGLSTSLPIDIQWAFYRSIEGLERVEIMRPAYAIEYDYVDPIQLHASLE
CCCCCCHHCCCCCCCCCCCEEEEHHHHHHHCHHHHHHHHCCCEEEEECCCCHHHHHHHHH
TKLVRNLYHAGQINGTSGYEEAAGQGLMAGINAALRVRDKEPLILGRSEAYIGVMIDDLV
HHHHHHHHHCCCCCCCCCHHHHHCCCHHHCCCEEEEEECCCCEEEECCCCEEEEEHHHHH
TLGTREPYRMFTSRAEYRLLLREDNADLRLRERGHAVGLVPEGAYQRFLEKRERIGAELA
HHCCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHH
RLKGTKLLPSEADPDFLAAFSLTALQNALTYEQLLRRPDITYEELCRIDPRAAEVPPVVR
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHC
EQVEIQIKYQGYIERQLDQVERARKLEGTRVPAGFDYGALPGLSAEVREKLAKFRPDTLG
CCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
QASRIQGVTPAAVGILSLALKARG
CHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA