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The map label for this gene is nirB [C]

Identifier: 78224667

GI number: 78224667

Start: 3911233

End: 3912918

Strand: Direct

Name: nirB [C]

Synonym: Gmet_3484

Alternate gene names: 78224667

Gene position: 3911233-3912918 (Clockwise)

Preceding gene: 78224657

Following gene: 78224668

Centisome position: 97.84

GC content: 61.33

Gene sequence:

>1686_bases
ATGAAACTGCGAGTTGTCGTGATCGGAGCCAACGCTGCGGGGGCAAAGGCCGCCTCCAAGGCAAAAAGGATGAACCCCCA
TGCGGAGGTTACTCTGATCGAGCGGGGCAATTTTATTTCTTACGGCGCCTGCGGTATTCCCTATTTCGTTTCGGATACCG
TAGCCGATGTAAAAGAGTTGATGAGCACCCCGGTCGGAGTGGTGCGAGATGCAGCCTTTTTCAACAAGGTGAAGGGAGTC
CACGTTATCACCGGAACCGAGGTGGTCAGCCTCGACCGGCAGTCGAGGACGGTCCAGCTGTTCGAAAAGGAGAGCGCGAA
AGCCTTCTCCCTTGAATACGACCGGCTGGTTCTGGCCACGGGGAGCTCGCCGTCCCTTCCACCGTTGAAGAACCGGGATC
TTGGCAACATCCTGACGGTCAAGGCCATTGAGGATGCGGAACTGCTGAAAGAGCGGGCCGTGACGGGAAAGAGAGCCTGT
ATCGTCGGCGGCGGTCTGATCGGCCTGGAAACGGCAGAGGCCTTGAGGCAGAAGGGGTTGGAGGTCACGATCGTCGAGAT
GCGCGACCAGCTTCTGCCGGGAATACTGGACCCGGAGATGGCGAGTCTGGTTGAAAGGCATATCCGTCAGAACGGTGTCG
AGGTTGAGACTTCTTGCCGGGTGGCCGGTTTTGACGGCACCGGAGTAGTGAATAAAGTTCTGACCGAACGGGGAGAGATC
CCGGCTGACGTGGTGGTACTGGCACCAGGGGTGACGCCGAACGTGAAGCTGGCGAAGGAGGCCGGATTGGAGATCGGCCC
CACTGGCGCCATCGCCGTCGACTCCCGCATGGGGACCAGCGACCCGGCAATCTACGCCTGCGGTGACTGCTGCGAAACCA
CCCACTTGGTTACCGGGAAAAAGGTTCATGTCCCCCTCGGCAGCACCGCCAACAAGCAGGGGAGGGTCGCCGGGATAAAC
GCATCCGGGGGGGAGGCAACCTTTGCCGGAATCCTCGGCACCAGCATCCTCAGGGTCTTCAGTGTCAACGCCGGGAAGAC
CGGCCTCACTGAATCCGAGGCGAGGGCACAGGGGTATGACGTCGAAACCGTCCTCTCGCCGGCCCCCGACAAAGCGCACT
TTTTCCCCGGCGCGAAACCGATCGCCCTAAAGCTCGTGGCAGACCGGGCTACCGGGAGGATTCTCGGGCTTCAGGCTGTC
GGCGAGGGGGCCGTGGACAAGCGGGTGGACGCCGCAGCCGCGGCCATTACGTTCGGCGCCACCGCCGACCAGCTTTCGCA
CCTGGATCTGGCCTACGCACCCCCCTACTCAGCCGCCATGGACAACCTCATCGTGGCCGCGGATATCCTGAAGAACAAAC
TGGCCGGCCACGCCAGGGGTATCTCCCCCCGAAAGGTGAGGCAGATGCTGGATGATGGCGACGACTTCATCCTCCTCGAC
GTCCGCTCTCCCGCGGAACATGCAGAGGTCGGCATCGAGGGGGCCACGCTTATCCCCCTGGGGGCATTGAGGGAAAAGCT
GGAGAGTCTCCCCAAGGACAAAGAGATCGTCACCTTCTGCAAAATCAGCCTCAGAGGCTACGAGGCCCAGAAGATCCTCG
ATGCTGCCGGCTTCAGGGATGTCAAGTTCCTGGATGGCGGCATAATGACGTGGCCCTATGAACTTCGACGCGGCGGAGGA
GCCTGA

Upstream 100 bases:

>100_bases
TGTAACTGACAAAAGACGGTTTCAAAAATGACCTCAAAATGGTATTAGCTATATATTCGAATTGCACCATTCACAAGACT
TTAAGCTACGGAGGTCTCTC

Downstream 100 bases:

>100_bases
TCAGCCGGGCAATGCCGACACTCCCGCAAAATCCTGCTTTAGGAAGGGACTGATCCGCTGGCTGTTTGTCACCCGGGACT
TCAAGAATACTATTGACAAA

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: CoA-disulfide reductase; CoADR [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MKLRVVVIGANAAGAKAASKAKRMNPHAEVTLIERGNFISYGACGIPYFVSDTVADVKELMSTPVGVVRDAAFFNKVKGV
HVITGTEVVSLDRQSRTVQLFEKESAKAFSLEYDRLVLATGSSPSLPPLKNRDLGNILTVKAIEDAELLKERAVTGKRAC
IVGGGLIGLETAEALRQKGLEVTIVEMRDQLLPGILDPEMASLVERHIRQNGVEVETSCRVAGFDGTGVVNKVLTERGEI
PADVVVLAPGVTPNVKLAKEAGLEIGPTGAIAVDSRMGTSDPAIYACGDCCETTHLVTGKKVHVPLGSTANKQGRVAGIN
ASGGEATFAGILGTSILRVFSVNAGKTGLTESEARAQGYDVETVLSPAPDKAHFFPGAKPIALKLVADRATGRILGLQAV
GEGAVDKRVDAAAAAITFGATADQLSHLDLAYAPPYSAAMDNLIVAADILKNKLAGHARGISPRKVRQMLDDGDDFILLD
VRSPAEHAEVGIEGATLIPLGALREKLESLPKDKEIVTFCKISLRGYEAQKILDAAGFRDVKFLDGGIMTWPYELRRGGG
A

Sequences:

>Translated_561_residues
MKLRVVVIGANAAGAKAASKAKRMNPHAEVTLIERGNFISYGACGIPYFVSDTVADVKELMSTPVGVVRDAAFFNKVKGV
HVITGTEVVSLDRQSRTVQLFEKESAKAFSLEYDRLVLATGSSPSLPPLKNRDLGNILTVKAIEDAELLKERAVTGKRAC
IVGGGLIGLETAEALRQKGLEVTIVEMRDQLLPGILDPEMASLVERHIRQNGVEVETSCRVAGFDGTGVVNKVLTERGEI
PADVVVLAPGVTPNVKLAKEAGLEIGPTGAIAVDSRMGTSDPAIYACGDCCETTHLVTGKKVHVPLGSTANKQGRVAGIN
ASGGEATFAGILGTSILRVFSVNAGKTGLTESEARAQGYDVETVLSPAPDKAHFFPGAKPIALKLVADRATGRILGLQAV
GEGAVDKRVDAAAAAITFGATADQLSHLDLAYAPPYSAAMDNLIVAADILKNKLAGHARGISPRKVRQMLDDGDDFILLD
VRSPAEHAEVGIEGATLIPLGALREKLESLPKDKEIVTFCKISLRGYEAQKILDAAGFRDVKFLDGGIMTWPYELRRGGG
A
>Mature_561_residues
MKLRVVVIGANAAGAKAASKAKRMNPHAEVTLIERGNFISYGACGIPYFVSDTVADVKELMSTPVGVVRDAAFFNKVKGV
HVITGTEVVSLDRQSRTVQLFEKESAKAFSLEYDRLVLATGSSPSLPPLKNRDLGNILTVKAIEDAELLKERAVTGKRAC
IVGGGLIGLETAEALRQKGLEVTIVEMRDQLLPGILDPEMASLVERHIRQNGVEVETSCRVAGFDGTGVVNKVLTERGEI
PADVVVLAPGVTPNVKLAKEAGLEIGPTGAIAVDSRMGTSDPAIYACGDCCETTHLVTGKKVHVPLGSTANKQGRVAGIN
ASGGEATFAGILGTSILRVFSVNAGKTGLTESEARAQGYDVETVLSPAPDKAHFFPGAKPIALKLVADRATGRILGLQAV
GEGAVDKRVDAAAAAITFGATADQLSHLDLAYAPPYSAAMDNLIVAADILKNKLAGHARGISPRKVRQMLDDGDDFILLD
VRSPAEHAEVGIEGATLIPLGALREKLESLPKDKEIVTFCKISLRGYEAQKILDAAGFRDVKFLDGGIMTWPYELRRGGG
A

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=330, Percent_Identity=26.0606060606061, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI226437568, Length=330, Percent_Identity=26.0606060606061, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI65787454, Length=330, Percent_Identity=26.0606060606061, Blast_Score=110, Evalue=5e-24,
Organism=Escherichia coli, GI1789765, Length=406, Percent_Identity=27.0935960591133, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1789065, Length=290, Percent_Identity=28.9655172413793, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788892, Length=221, Percent_Identity=30.316742081448, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87082354, Length=305, Percent_Identity=28.5245901639344, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1786307, Length=441, Percent_Identity=26.3038548752834, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17559934, Length=224, Percent_Identity=29.4642857142857, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24639257, Length=323, Percent_Identity=22.9102167182663, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281359715, Length=323, Percent_Identity=22.9102167182663, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281359713, Length=323, Percent_Identity=22.9102167182663, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24639250, Length=323, Percent_Identity=22.9102167182663, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI18543267, Length=323, Percent_Identity=22.9102167182663, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24639252, Length=323, Percent_Identity=22.9102167182663, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24585130, Length=331, Percent_Identity=21.7522658610272, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017758
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.14 [H]

Molecular weight: Translated: 59144; Mature: 59144

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRVVVIGANAAGAKAASKAKRMNPHAEVTLIERGNFISYGACGIPYFVSDTVADVKEL
CEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHH
MSTPVGVVRDAAFFNKVKGVHVITGTEVVSLDRQSRTVQLFEKESAKAFSLEYDRLVLAT
HHCCHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCEEEEEECCCCCEEEEEECEEEEEE
GSSPSLPPLKNRDLGNILTVKAIEDAELLKERAVTGKRACIVGGGLIGLETAEALRQKGL
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEECCEEEHHHHHHHHHCCC
EVTIVEMRDQLLPGILDPEMASLVERHIRQNGVEVETSCRVAGFDGTGVVNKVLTERGEI
EEEEEEHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECEEEECCCCCHHHHHHHHHCCCC
PADVVVLAPGVTPNVKLAKEAGLEIGPTGAIAVDSRMGTSDPAIYACGDCCETTHLVTGK
CCCEEEECCCCCCCCEEHHHCCCEECCCCEEEECCCCCCCCCCEEECCCCCCCCEEECCC
KVHVPLGSTANKQGRVAGINASGGEATFAGILGTSILRVFSVNAGKTGLTESEARAQGYD
EEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCHHHHCCCC
VETVLSPAPDKAHFFPGAKPIALKLVADRATGRILGLQAVGEGAVDKRVDAAAAAITFGA
CCEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHEEECC
TADQLSHLDLAYAPPYSAAMDNLIVAADILKNKLAGHARGISPRKVRQMLDDGDDFILLD
CHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEE
VRSPAEHAEVGIEGATLIPLGALREKLESLPKDKEIVTFCKISLRGYEAQKILDAAGFRD
ECCCHHHHHCCCCCCEEECHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHCCCCC
VKFLDGGIMTWPYELRRGGGA
EEEECCCEEECCHHHHCCCCC
>Mature Secondary Structure
MKLRVVVIGANAAGAKAASKAKRMNPHAEVTLIERGNFISYGACGIPYFVSDTVADVKEL
CEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHH
MSTPVGVVRDAAFFNKVKGVHVITGTEVVSLDRQSRTVQLFEKESAKAFSLEYDRLVLAT
HHCCHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCEEEEEECCCCCEEEEEECEEEEEE
GSSPSLPPLKNRDLGNILTVKAIEDAELLKERAVTGKRACIVGGGLIGLETAEALRQKGL
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEECCEEEHHHHHHHHHCCC
EVTIVEMRDQLLPGILDPEMASLVERHIRQNGVEVETSCRVAGFDGTGVVNKVLTERGEI
EEEEEEHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECEEEECCCCCHHHHHHHHHCCCC
PADVVVLAPGVTPNVKLAKEAGLEIGPTGAIAVDSRMGTSDPAIYACGDCCETTHLVTGK
CCCEEEECCCCCCCCEEHHHCCCEECCCCEEEECCCCCCCCCCEEECCCCCCCCEEECCC
KVHVPLGSTANKQGRVAGINASGGEATFAGILGTSILRVFSVNAGKTGLTESEARAQGYD
EEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCHHHHCCCC
VETVLSPAPDKAHFFPGAKPIALKLVADRATGRILGLQAVGEGAVDKRVDAAAAAITFGA
CCEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHEEECC
TADQLSHLDLAYAPPYSAAMDNLIVAADILKNKLAGHARGISPRKVRQMLDDGDDFILLD
CHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEE
VRSPAEHAEVGIEGATLIPLGALREKLESLPKDKEIVTFCKISLRGYEAQKILDAAGFRD
ECCCHHHHHCCCCCCEEECHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHCCCCC
VKFLDGGIMTWPYELRRGGGA
EEEECCCEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA