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The map label for this gene is phoR [H]

Identifier: 78224572

GI number: 78224572

Start: 3795711

End: 3797123

Strand: Reverse

Name: phoR [H]

Synonym: Gmet_3382

Alternate gene names: 78224572

Gene position: 3797123-3795711 (Counterclockwise)

Preceding gene: 78224573

Following gene: 78224571

Centisome position: 94.99

GC content: 62.92

Gene sequence:

>1413_bases
TTGTTCTTCCGGTCGATCCGCTTTTCCCTTACCCTCTGGTATGCGGTCACCCTGGCGGTCATCCTCGTTCTGTTCAGCTC
GTTCATCTACCTTATCCTTCAGAACCAGCTCACCAAGGAGATCGACCGGGAACTTCTGACCGTTGCCGAGGCCGTGGCGA
GCCCGACGCTGGAGCCCTTCCGGCGTGCGGGCCCGTCGGTCTTCGATCAGGTTCTGGAGGACTTCATCGGCACCCGTCTG
ACCGGTAAGTTCGTCCAGGTCCTCGACGGGGGTGGGCGGATCACTGCGTCGTCCAAGGGGGCCGAGGGGCTCCGGACTCC
CCTTGGCAAGGGAGCTTCCCGCCGGGCGTGGGCCGGTAAGGTGACCTACGAAACCAAGGTCAGCCTCGATCTCTATCCGG
TCCGCACCATTACCTATCCGATCATGGATAACGGCAGGCTACTGCAGATCGTCCAGGTCGGCACCTCCATGCGGGCCGCT
GCCGAAACCCTCGACAAGGTTCTCGTCGTCTTTGCCGTTTCCATTCCCTTGGCGCTTATCCTCTGGAGCGTCGGGGGATG
GTTCCTGGCGGGACGGGCGCTGAAACCGGTCGATTTCATCACCCGCAGCGCCCAGAAGATCACGGCCGAAAACCTGGGGC
TGCGGCTTGAAGTGGTCAATCCCCAAGACGAAATCGGGCGTCTCGCGATAACCTTCAACGACACCCTTGAGCGCCTTGAG
GACGCCTTCCGGCGGGTGAAGCAGTTCACCGGCGACGTCTCCCACGAGCTTCGGACCCCCCTCACCATCCTCCGGGGAGA
GACCGAGGTGGGGCTGCGCTGGGCTCGGGAGCCGGAGGAGTTCCGGGAGCTTTTCCGGAGCAACCTGGAAGAGATCAACC
GGATGTCGAAGATCATCGAGGCGCTCCTGGAGCTATCCCGTGCCGAAGAGGGAGGGCTCAAGCTGGAGCGTGTCCCCATT
GAACTGGAGGACCTCCTGGGAGAGCTGGTCCAGCAGTCCCGCCTCATTGACCCGGAAAAGGGACTCAAGATTGCCTTCTC
GGCCGTGACGCCGGTCACTGTCACGGGGGACTGGCTCCGGCTGCGTCAGGTATTCATGAACCTCCTCGACAACGCCGTCA
CCTATACCCCTGCCGGCGGAGAGGTGTCGGTGGTCCTCGACGCCAGCGGCGGATTTGCCCGGGTGACCGTCATCGACAGC
GGCGTCGGCATCCCGGCCGATGACCTCCCCCACATCTTCGAGCGATTCTACCGGGTCGACAAGGCCCGTAACCGGGCCGA
CGGTGGCTGTGGTCTCGGTCTCTCTCTGGTTCGCACTATCACGGAGGCCCATGGCGGAAAGATCGACGTGGTGAGTGAGG
TGGGGAAGGGGAGCGTCTTTACGGTGTATCTCCCCCTTCAGGGTACGGTCTGA

Upstream 100 bases:

>100_bases
GTGAACTACCTGCGGAAGAAGATCGACCGCGAGGCCGACAAGAAGCTGATCCACACGGTACGGGGCGTGGGCTACATCCT
GAAGGAAGAGGAGTGACCCG

Downstream 100 bases:

>100_bases
CGGAGCGCACTCCCCCATGGATTCCATTATCATTGCCGCGGCAACCAGTCTGGAGCTTTCCCTCCTCGTCCGGAGCATCG
GTGCCCGGGAGGTCAGGAGA

Product: heavy metal sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MFFRSIRFSLTLWYAVTLAVILVLFSSFIYLILQNQLTKEIDRELLTVAEAVASPTLEPFRRAGPSVFDQVLEDFIGTRL
TGKFVQVLDGGGRITASSKGAEGLRTPLGKGASRRAWAGKVTYETKVSLDLYPVRTITYPIMDNGRLLQIVQVGTSMRAA
AETLDKVLVVFAVSIPLALILWSVGGWFLAGRALKPVDFITRSAQKITAENLGLRLEVVNPQDEIGRLAITFNDTLERLE
DAFRRVKQFTGDVSHELRTPLTILRGETEVGLRWAREPEEFRELFRSNLEEINRMSKIIEALLELSRAEEGGLKLERVPI
ELEDLLGELVQQSRLIDPEKGLKIAFSAVTPVTVTGDWLRLRQVFMNLLDNAVTYTPAGGEVSVVLDASGGFARVTVIDS
GVGIPADDLPHIFERFYRVDKARNRADGGCGLGLSLVRTITEAHGGKIDVVSEVGKGSVFTVYLPLQGTV

Sequences:

>Translated_470_residues
MFFRSIRFSLTLWYAVTLAVILVLFSSFIYLILQNQLTKEIDRELLTVAEAVASPTLEPFRRAGPSVFDQVLEDFIGTRL
TGKFVQVLDGGGRITASSKGAEGLRTPLGKGASRRAWAGKVTYETKVSLDLYPVRTITYPIMDNGRLLQIVQVGTSMRAA
AETLDKVLVVFAVSIPLALILWSVGGWFLAGRALKPVDFITRSAQKITAENLGLRLEVVNPQDEIGRLAITFNDTLERLE
DAFRRVKQFTGDVSHELRTPLTILRGETEVGLRWAREPEEFRELFRSNLEEINRMSKIIEALLELSRAEEGGLKLERVPI
ELEDLLGELVQQSRLIDPEKGLKIAFSAVTPVTVTGDWLRLRQVFMNLLDNAVTYTPAGGEVSVVLDASGGFARVTVIDS
GVGIPADDLPHIFERFYRVDKARNRADGGCGLGLSLVRTITEAHGGKIDVVSEVGKGSVFTVYLPLQGTV
>Mature_470_residues
MFFRSIRFSLTLWYAVTLAVILVLFSSFIYLILQNQLTKEIDRELLTVAEAVASPTLEPFRRAGPSVFDQVLEDFIGTRL
TGKFVQVLDGGGRITASSKGAEGLRTPLGKGASRRAWAGKVTYETKVSLDLYPVRTITYPIMDNGRLLQIVQVGTSMRAA
AETLDKVLVVFAVSIPLALILWSVGGWFLAGRALKPVDFITRSAQKITAENLGLRLEVVNPQDEIGRLAITFNDTLERLE
DAFRRVKQFTGDVSHELRTPLTILRGETEVGLRWAREPEEFRELFRSNLEEINRMSKIIEALLELSRAEEGGLKLERVPI
ELEDLLGELVQQSRLIDPEKGLKIAFSAVTPVTVTGDWLRLRQVFMNLLDNAVTYTPAGGEVSVVLDASGGFARVTVIDS
GVGIPADDLPHIFERFYRVDKARNRADGGCGLGLSLVRTITEAHGGKIDVVSEVGKGSVFTVYLPLQGTV

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=335, Percent_Identity=31.9402985074627, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1788393, Length=276, Percent_Identity=37.3188405797101, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI1786600, Length=257, Percent_Identity=33.0739299610895, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=32.9059829059829, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788279, Length=295, Percent_Identity=28.8135593220339, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1789149, Length=237, Percent_Identity=31.6455696202532, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1787894, Length=309, Percent_Identity=30.0970873786408, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI145693157, Length=278, Percent_Identity=29.4964028776978, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786912, Length=255, Percent_Identity=29.4117647058824, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=31.9327731092437, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790346, Length=274, Percent_Identity=30.2919708029197, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1790861, Length=334, Percent_Identity=27.5449101796407, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1789403, Length=289, Percent_Identity=30.7958477508651, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790551, Length=299, Percent_Identity=28.7625418060201, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788549, Length=257, Percent_Identity=28.7937743190661, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082128, Length=313, Percent_Identity=27.4760383386581, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081816, Length=251, Percent_Identity=30.6772908366534, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787374, Length=322, Percent_Identity=26.3975155279503, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788713, Length=226, Percent_Identity=24.3362831858407, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789808, Length=242, Percent_Identity=29.7520661157025, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790300, Length=328, Percent_Identity=26.8292682926829, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51891; Mature: 51891

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFRSIRFSLTLWYAVTLAVILVLFSSFIYLILQNQLTKEIDRELLTVAEAVASPTLEPF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RRAGPSVFDQVLEDFIGTRLTGKFVQVLDGGGRITASSKGAEGLRTPLGKGASRRAWAGK
HHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCHHCCCCCCCCCCCCCCC
VTYETKVSLDLYPVRTITYPIMDNGRLLQIVQVGTSMRAAAETLDKVLVVFAVSIPLALI
EEEEEEEEEEEEEEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LWSVGGWFLAGRALKPVDFITRSAQKITAENLGLRLEVVNPQDEIGRLAITFNDTLERLE
HHHHCHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCEEEEEEHHHHHHHH
DAFRRVKQFTGDVSHELRTPLTILRGETEVGLRWAREPEEFRELFRSNLEEINRMSKIIE
HHHHHHHHHHCHHHHHHHCCHHEECCCHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHH
ALLELSRAEEGGLKLERVPIELEDLLGELVQQSRLIDPEKGLKIAFSAVTPVTVTGDWLR
HHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEECHHHHH
LRQVFMNLLDNAVTYTPAGGEVSVVLDASGGFARVTVIDSGVGIPADDLPHIFERFYRVD
HHHHHHHHHCCCEEECCCCCEEEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHH
KARNRADGGCGLGLSLVRTITEAHGGKIDVVSEVGKGSVFTVYLPLQGTV
HHCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MFFRSIRFSLTLWYAVTLAVILVLFSSFIYLILQNQLTKEIDRELLTVAEAVASPTLEPF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RRAGPSVFDQVLEDFIGTRLTGKFVQVLDGGGRITASSKGAEGLRTPLGKGASRRAWAGK
HHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCHHCCCCCCCCCCCCCCC
VTYETKVSLDLYPVRTITYPIMDNGRLLQIVQVGTSMRAAAETLDKVLVVFAVSIPLALI
EEEEEEEEEEEEEEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LWSVGGWFLAGRALKPVDFITRSAQKITAENLGLRLEVVNPQDEIGRLAITFNDTLERLE
HHHHCHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCEEEEEEHHHHHHHH
DAFRRVKQFTGDVSHELRTPLTILRGETEVGLRWAREPEEFRELFRSNLEEINRMSKIIE
HHHHHHHHHHCHHHHHHHCCHHEECCCHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHH
ALLELSRAEEGGLKLERVPIELEDLLGELVQQSRLIDPEKGLKIAFSAVTPVTVTGDWLR
HHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEECHHHHH
LRQVFMNLLDNAVTYTPAGGEVSVVLDASGGFARVTVIDSGVGIPADDLPHIFERFYRVD
HHHHHHHHHCCCEEECCCCCEEEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHH
KARNRADGGCGLGLSLVRTITEAHGGKIDVVSEVGKGSVFTVYLPLQGTV
HHCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]