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The map label for this gene is nuoM [H]

Identifier: 78224533

GI number: 78224533

Start: 3759037

End: 3760596

Strand: Reverse

Name: nuoM [H]

Synonym: Gmet_3343

Alternate gene names: 78224533

Gene position: 3760596-3759037 (Counterclockwise)

Preceding gene: 78224534

Following gene: 78224532

Centisome position: 94.08

GC content: 50.64

Gene sequence:

>1560_bases
ATGAGTCAGTTTCCCTTAATCAGCGTGATGACCTTTCTACCACTTCTAGGGGTCGTACTGCTCTTCTTCGTTCCCAAGCA
GAGCCATTCTGCACAGCGGGGGCTTGCACTTCTGGTGGCTCTTGCGACGTTTGTCGTATCACTTCCGCTTGTTACCGGCT
TCCAGAGCAACGCTGAGTTCCAGTTTGTGGAGAAGGTACCCTGGATCGCTGCCGGCCCGTACCAGATGAATTATCATGTT
GCCATCGATGGAATCAGTCTCTGGCTCGTGATTCTGACAACCTTCATCGTGCCAATCGCCATTCTTTCAACCTGGACAGC
CGTTGAGAACAAGGTGAAGGAATACATGATCTGTCTCCTCCTCCTTGAGGTCGGGATGCTTGGCGCTTTCGTTTCACTTG
ACCTGTTCCTCTTCTACATATTTTGGGAGGTCATGCTCATTCCAATGTACTTCATCATCGGTATATGGGGTGGGAAAAAC
AAGATATACGCTGCGCTCAAGTTCTTTATCTATACAATGGTCGGTTCGCTCCTGATGCTGATCGCTCTTGTCGTCCTTTA
CTTTAAGGGCGGAGCCGGCGACTTCAGTGTGCTTCGCTTCTATGATCTCGCTCTCGATCCTGCGACCCAGACCTGGATGT
TCCTTGCGTTCGCTTTGGCCTTTGCCATTAAAGTACCGATGTTCCCGCTGCATACGTGGTTGCCGGATGCACATACCGAA
GCCCCGACCGCTGGTTCCGTAATTCTGGCTGCCGTTCTCCTTAAGATGGGAACCTATGGTTTTGTACGCTTCGCCATGCC
GCTTTTCCCGGACGCTACAGCTCAGTTTACTCCTCTCTTTGCAGTCCTTTCAGTGATAGGTATTGTGTATGCTGCCCTTG
TGGCTATGGTTCAGGAGGACGTAAAAAAACTTGTTGCCTACTCCTCAGTTGCACACCTTGGCTATGTTATGCTCGGCGTG
TTTGCGCTCAACCAGCAAGGACTCTCGGGCGGAATGATTCAGATGCTCAATCACGGTGTTTCCACTGGCGCACTGTTCCT
TATAGTTGGTTTCATCTATGAGCGTCGTCACACTCGTCTCATAACTGATTTCGGCGGATTGGCCAAGCAGATGCCGGTTT
TTGCTACAATTTTCATGATTGTCACCCTTTCTTCCATCGGTCTTCCGGGAACCAACGGCTTTGTTGGAGAGTTCCTTATT
CTCATCGGTGCGTATGAAAGTGAACTGCGTTGGTATGCTGTAATCGGGACGAGCGGCGTGATTCTTTCCGCAGTTTATAT
GCTCTGGATGTTCCAGCGTGTCATGTTTGGAGAGCTGAATAACCCCAAGAACCAGACGCTTACAGATTTGAATGCACGCG
AAGTGGTGATTATGCTGCCGTTGCTCGTGTTGATCTTCGTTATGGGCGTGTATCCTACCCCCTTCATCGACAAAATGAGC
CCATCGCTTGAGAAATTGATCGTTCACGTCAAGTCGAAGCAGACCGCCAAGGCACAGATTCCTCAGATCCCGGCACCGCA
GGCTCTTCCTGCCGCCGTGCTGCCTGTTGAGCACAAGTAA

Upstream 100 bases:

>100_bases
ACAACTATGCCCTTGGCATGTCACTCGGTGTTGTAGTTATCGTTGCCTTCTATCTCTTCCGCTAACTCTGAATTACCAAT
AGGACTAAAGGAGCAGATTC

Downstream 100 bases:

>100_bases
TCGGTTTCAGAAAAGCCACCGGAGGGAACCTAGATGGAAACAATTGCAATTCCTGCCATTAACTTCGCACCAATAATGCC
AGAGGTCATACTTTCGATCT

Product: proton-translocating NADH-quinone oxidoreductase, chain M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSQFPLISVMTFLPLLGVVLLFFVPKQSHSAQRGLALLVALATFVVSLPLVTGFQSNAEFQFVEKVPWIAAGPYQMNYHV
AIDGISLWLVILTTFIVPIAILSTWTAVENKVKEYMICLLLLEVGMLGAFVSLDLFLFYIFWEVMLIPMYFIIGIWGGKN
KIYAALKFFIYTMVGSLLMLIALVVLYFKGGAGDFSVLRFYDLALDPATQTWMFLAFALAFAIKVPMFPLHTWLPDAHTE
APTAGSVILAAVLLKMGTYGFVRFAMPLFPDATAQFTPLFAVLSVIGIVYAALVAMVQEDVKKLVAYSSVAHLGYVMLGV
FALNQQGLSGGMIQMLNHGVSTGALFLIVGFIYERRHTRLITDFGGLAKQMPVFATIFMIVTLSSIGLPGTNGFVGEFLI
LIGAYESELRWYAVIGTSGVILSAVYMLWMFQRVMFGELNNPKNQTLTDLNAREVVIMLPLLVLIFVMGVYPTPFIDKMS
PSLEKLIVHVKSKQTAKAQIPQIPAPQALPAAVLPVEHK

Sequences:

>Translated_519_residues
MSQFPLISVMTFLPLLGVVLLFFVPKQSHSAQRGLALLVALATFVVSLPLVTGFQSNAEFQFVEKVPWIAAGPYQMNYHV
AIDGISLWLVILTTFIVPIAILSTWTAVENKVKEYMICLLLLEVGMLGAFVSLDLFLFYIFWEVMLIPMYFIIGIWGGKN
KIYAALKFFIYTMVGSLLMLIALVVLYFKGGAGDFSVLRFYDLALDPATQTWMFLAFALAFAIKVPMFPLHTWLPDAHTE
APTAGSVILAAVLLKMGTYGFVRFAMPLFPDATAQFTPLFAVLSVIGIVYAALVAMVQEDVKKLVAYSSVAHLGYVMLGV
FALNQQGLSGGMIQMLNHGVSTGALFLIVGFIYERRHTRLITDFGGLAKQMPVFATIFMIVTLSSIGLPGTNGFVGEFLI
LIGAYESELRWYAVIGTSGVILSAVYMLWMFQRVMFGELNNPKNQTLTDLNAREVVIMLPLLVLIFVMGVYPTPFIDKMS
PSLEKLIVHVKSKQTAKAQIPQIPAPQALPAAVLPVEHK
>Mature_518_residues
SQFPLISVMTFLPLLGVVLLFFVPKQSHSAQRGLALLVALATFVVSLPLVTGFQSNAEFQFVEKVPWIAAGPYQMNYHVA
IDGISLWLVILTTFIVPIAILSTWTAVENKVKEYMICLLLLEVGMLGAFVSLDLFLFYIFWEVMLIPMYFIIGIWGGKNK
IYAALKFFIYTMVGSLLMLIALVVLYFKGGAGDFSVLRFYDLALDPATQTWMFLAFALAFAIKVPMFPLHTWLPDAHTEA
PTAGSVILAAVLLKMGTYGFVRFAMPLFPDATAQFTPLFAVLSVIGIVYAALVAMVQEDVKKLVAYSSVAHLGYVMLGVF
ALNQQGLSGGMIQMLNHGVSTGALFLIVGFIYERRHTRLITDFGGLAKQMPVFATIFMIVTLSSIGLPGTNGFVGEFLIL
IGAYESELRWYAVIGTSGVILSAVYMLWMFQRVMFGELNNPKNQTLTDLNAREVVIMLPLLVLIFVMGVYPTPFIDKMSP
SLEKLIVHVKSKQTAKAQIPQIPAPQALPAAVLPVEHK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=371, Percent_Identity=32.8840970350404, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI251831117, Length=456, Percent_Identity=25.4385964912281, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788613, Length=498, Percent_Identity=37.3493975903614, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1788827, Length=293, Percent_Identity=31.0580204778157, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1788831, Length=368, Percent_Identity=28.804347826087, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788614, Length=476, Percent_Identity=27.3109243697479, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI145693160, Length=435, Percent_Identity=25.0574712643678, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788829, Length=399, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI2367154, Length=283, Percent_Identity=27.208480565371, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 57261; Mature: 57129

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQFPLISVMTFLPLLGVVLLFFVPKQSHSAQRGLALLVALATFVVSLPLVTGFQSNAEF
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
QFVEKVPWIAAGPYQMNYHVAIDGISLWLVILTTFIVPIAILSTWTAVENKVKEYMICLL
EHHHHCCCEECCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEVGMLGAFVSLDLFLFYIFWEVMLIPMYFIIGIWGGKNKIYAALKFFIYTMVGSLLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IALVVLYFKGGAGDFSVLRFYDLALDPATQTWMFLAFALAFAIKVPMFPLHTWLPDAHTE
HHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
APTAGSVILAAVLLKMGTYGFVRFAMPLFPDATAQFTPLFAVLSVIGIVYAALVAMVQED
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VKKLVAYSSVAHLGYVMLGVFALNQQGLSGGMIQMLNHGVSTGALFLIVGFIYERRHTRL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ITDFGGLAKQMPVFATIFMIVTLSSIGLPGTNGFVGEFLILIGAYESELRWYAVIGTSGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHCCEEEEEECCCHH
ILSAVYMLWMFQRVMFGELNNPKNQTLTDLNAREVVIMLPLLVLIFVMGVYPTPFIDKMS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCC
PSLEKLIVHVKSKQTAKAQIPQIPAPQALPAAVLPVEHK
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]