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The map label for this gene is nuoN [H]

Identifier: 78224532

GI number: 78224532

Start: 3757549

End: 3759003

Strand: Reverse

Name: nuoN [H]

Synonym: Gmet_3342

Alternate gene names: 78224532

Gene position: 3759003-3757549 (Counterclockwise)

Preceding gene: 78224533

Following gene: 78224527

Centisome position: 94.04

GC content: 49.14

Gene sequence:

>1455_bases
ATGGAAACAATTGCAATTCCTGCCATTAACTTCGCACCAATAATGCCAGAGGTCATACTTTCGATCTTTGCGATGGTGCT
GCTCCTTGTAAACGTATTCGTTCCGAGTGAGCACAAGGCCTATCTGGGATATTTGAGTCTCGTAGGCGTCATTGTCGCCG
GATTCACGCTCATTGGTGGATGGGGGGTACCTCTCTCGGCGTTCAATGATTCTGTTGTTCAGGATAACTTCTCTATATTT
TTCAAGGGCATTTTCCTCCTCACGGTTGCGATGAGCATTCTTATCTCGGATCAATATTTCAAGCGTGAAGAATGCAATCT
CGGGGAACTTTACCCACTGCTTCTCTTTGCCACGGTTGGTATGATGCTTATGGCGTCTGGTACGGATTTGATGACTATTT
TTCTTGGTCTCGAAGTGCTCTCCGTATCTCTGTATATTTTGGCAGGCTTTAACAGGGCCAATGTTAAGTCGAATGAAGCT
GGCCTCAAGTACTTTCTTCTTGGCGCGTTTTCAACTGGATTCCTCCTGTACGGAATGGCCCTCACCTACGGCGCTACCGG
CACTACGCGCGTAGTGAAGATTGCTGAGTATGTGTCGCAAAACGGAGTCGTGGCGCAGAATCCTATGTTTGTGATCGGAA
TGCTGCTGATGGCGGTAGGCTTTTCCTTCAAGATCGCCGCTGCTCCGTTCCATATGTGGACTCCGGATGTCTACGAGGGT
GCTCCGACTCCAATGACCGCTTTCATGTCAGCGGGGCCGAAGGCAGCCGGTTTCGCAGCATTTATGCGCGTTGTTATTAT
TGCTTTCCCTTCGCTTAAGGCTGACTGGTCCGATCTCCTCTGGATTCTTGCCGTTCTTACCATGACCATTGGTAACCTGA
TTGCTCTCAATCAGGACAATATCAAGAGGATGCTCGCATACTCATCGATTGCGCACGCTGGGTATGCTCTGGTCGGTTTT
GCCGCCGGAAATGCAGAGGGGACGGCAGGCATTCTCTTCTACATGCTTTCCTATGCTTTTATGAATATAGGGGCCTTTGC
CATTATCGTTCTTGTTGGCAAGAAGGGCGAAGCAAACAATAATGTTATGGATTACGCAGGGTTCGGAACTAAGCACCCGG
TGCTGGCCCTGGCAATGGCAGTCTTCCTCTTCTCGCTGGCTGGCATACCGCCGACTGCTGGTTTTATCGGTAAGTTCTAC
CTCTTCTCTGGCGCCATAAAGGCTGGGTATGTATGGCTTGCCATCATCGGGGTACTGAATAGTGCCGCGTCGGTATATTA
CTATCTCCGTGTGATGGTTTATATGTATATGAAGGATCCTGTTGAAGAGTTTGACTGGATGAAGCTTACTCCGGCAGTTG
CCATCTGCATTATTATTGCTGTGGTTGGTGTCCTGTTGCCAGGAGTTGTTCCAGCGTTTCTCCTCAACCTTGCACAGCAG
GCAGTTCTCCTTTAA

Upstream 100 bases:

>100_bases
ACAGATTCCTCAGATCCCGGCACCGCAGGCTCTTCCTGCCGCCGTGCTGCCTGTTGAGCACAAGTAATCGGTTTCAGAAA
AGCCACCGGAGGGAACCTAG

Downstream 100 bases:

>100_bases
CTAGAAGTAAGTAATAGGCTTTTCCCTCAGGGCTCCTTTATATGAAGGAGCCCTTTTGTATTTTGTAAGAAGAATCGGCA
TGGCACGGAATAACTGGGTG

Product: proton-translocating NADH-quinone oxidoreductase, chain N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
METIAIPAINFAPIMPEVILSIFAMVLLLVNVFVPSEHKAYLGYLSLVGVIVAGFTLIGGWGVPLSAFNDSVVQDNFSIF
FKGIFLLTVAMSILISDQYFKREECNLGELYPLLLFATVGMMLMASGTDLMTIFLGLEVLSVSLYILAGFNRANVKSNEA
GLKYFLLGAFSTGFLLYGMALTYGATGTTRVVKIAEYVSQNGVVAQNPMFVIGMLLMAVGFSFKIAAAPFHMWTPDVYEG
APTPMTAFMSAGPKAAGFAAFMRVVIIAFPSLKADWSDLLWILAVLTMTIGNLIALNQDNIKRMLAYSSIAHAGYALVGF
AAGNAEGTAGILFYMLSYAFMNIGAFAIIVLVGKKGEANNNVMDYAGFGTKHPVLALAMAVFLFSLAGIPPTAGFIGKFY
LFSGAIKAGYVWLAIIGVLNSAASVYYYLRVMVYMYMKDPVEEFDWMKLTPAVAICIIIAVVGVLLPGVVPAFLLNLAQQ
AVLL

Sequences:

>Translated_484_residues
METIAIPAINFAPIMPEVILSIFAMVLLLVNVFVPSEHKAYLGYLSLVGVIVAGFTLIGGWGVPLSAFNDSVVQDNFSIF
FKGIFLLTVAMSILISDQYFKREECNLGELYPLLLFATVGMMLMASGTDLMTIFLGLEVLSVSLYILAGFNRANVKSNEA
GLKYFLLGAFSTGFLLYGMALTYGATGTTRVVKIAEYVSQNGVVAQNPMFVIGMLLMAVGFSFKIAAAPFHMWTPDVYEG
APTPMTAFMSAGPKAAGFAAFMRVVIIAFPSLKADWSDLLWILAVLTMTIGNLIALNQDNIKRMLAYSSIAHAGYALVGF
AAGNAEGTAGILFYMLSYAFMNIGAFAIIVLVGKKGEANNNVMDYAGFGTKHPVLALAMAVFLFSLAGIPPTAGFIGKFY
LFSGAIKAGYVWLAIIGVLNSAASVYYYLRVMVYMYMKDPVEEFDWMKLTPAVAICIIIAVVGVLLPGVVPAFLLNLAQQ
AVLL
>Mature_484_residues
METIAIPAINFAPIMPEVILSIFAMVLLLVNVFVPSEHKAYLGYLSLVGVIVAGFTLIGGWGVPLSAFNDSVVQDNFSIF
FKGIFLLTVAMSILISDQYFKREECNLGELYPLLLFATVGMMLMASGTDLMTIFLGLEVLSVSLYILAGFNRANVKSNEA
GLKYFLLGAFSTGFLLYGMALTYGATGTTRVVKIAEYVSQNGVVAQNPMFVIGMLLMAVGFSFKIAAAPFHMWTPDVYEG
APTPMTAFMSAGPKAAGFAAFMRVVIIAFPSLKADWSDLLWILAVLTMTIGNLIALNQDNIKRMLAYSSIAHAGYALVGF
AAGNAEGTAGILFYMLSYAFMNIGAFAIIVLVGKKGEANNNVMDYAGFGTKHPVLALAMAVFLFSLAGIPPTAGFIGKFY
LFSGAIKAGYVWLAIIGVLNSAASVYYYLRVMVYMYMKDPVEEFDWMKLTPAVAICIIIAVVGVLLPGVVPAFLLNLAQQ
AVLL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=418, Percent_Identity=35.4066985645933, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1788831, Length=392, Percent_Identity=23.9795918367347, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788614, Length=386, Percent_Identity=23.8341968911917, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788827, Length=374, Percent_Identity=23.7967914438503, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788613, Length=196, Percent_Identity=25, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI2367154, Length=147, Percent_Identity=29.2517006802721, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 52097; Mature: 52097

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIAIPAINFAPIMPEVILSIFAMVLLLVNVFVPSEHKAYLGYLSLVGVIVAGFTLIGG
CCCEEECCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
WGVPLSAFNDSVVQDNFSIFFKGIFLLTVAMSILISDQYFKREECNLGELYPLLLFATVG
CCCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MMLMASGTDLMTIFLGLEVLSVSLYILAGFNRANVKSNEAGLKYFLLGAFSTGFLLYGMA
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
LTYGATGTTRVVKIAEYVSQNGVVAQNPMFVIGMLLMAVGFSFKIAAAPFHMWTPDVYEG
HHCCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHCCC
APTPMTAFMSAGPKAAGFAAFMRVVIIAFPSLKADWSDLLWILAVLTMTIGNLIALNQDN
CCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCEEEECHHH
IKRMLAYSSIAHAGYALVGFAAGNAEGTAGILFYMLSYAFMNIGAFAIIVLVGKKGEANN
HHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
NVMDYAGFGTKHPVLALAMAVFLFSLAGIPPTAGFIGKFYLFSGAIKAGYVWLAIIGVLN
CEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAASVYYYLRVMVYMYMKDPVEEFDWMKLTPAVAICIIIAVVGVLLPGVVPAFLLNLAQQ
HHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLL
HHCC
>Mature Secondary Structure
METIAIPAINFAPIMPEVILSIFAMVLLLVNVFVPSEHKAYLGYLSLVGVIVAGFTLIGG
CCCEEECCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
WGVPLSAFNDSVVQDNFSIFFKGIFLLTVAMSILISDQYFKREECNLGELYPLLLFATVG
CCCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MMLMASGTDLMTIFLGLEVLSVSLYILAGFNRANVKSNEAGLKYFLLGAFSTGFLLYGMA
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
LTYGATGTTRVVKIAEYVSQNGVVAQNPMFVIGMLLMAVGFSFKIAAAPFHMWTPDVYEG
HHCCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHCCC
APTPMTAFMSAGPKAAGFAAFMRVVIIAFPSLKADWSDLLWILAVLTMTIGNLIALNQDN
CCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCEEEECHHH
IKRMLAYSSIAHAGYALVGFAAGNAEGTAGILFYMLSYAFMNIGAFAIIVLVGKKGEANN
HHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
NVMDYAGFGTKHPVLALAMAVFLFSLAGIPPTAGFIGKFYLFSGAIKAGYVWLAIIGVLN
CEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAASVYYYLRVMVYMYMKDPVEEFDWMKLTPAVAICIIIAVVGVLLPGVVPAFLLNLAQQ
HHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA