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The map label for this gene is ttcA

Identifier: 78224527

GI number: 78224527

Start: 3750195

End: 3750989

Strand: Reverse

Name: ttcA

Synonym: Gmet_3336

Alternate gene names: 78224527

Gene position: 3750989-3750195 (Counterclockwise)

Preceding gene: 78224532

Following gene: 78224526

Centisome position: 93.84

GC content: 63.65

Gene sequence:

>795_bases
TTGTGGAAGGAGAAGGAATCCGTGGAGTTTGTGGACGAAAAGCGGTATCGGAAAATAAAGAACGGGGTCGGCCGGGCCGT
CGCCGACTTCAACCTCATCGAGGAAGGGGACCGGATTGCGGTGGCGGTCTCGGGGGGGAAGGATTCCTACACTCTCCTCC
ATATCCTGGAGGCGCTCCGGCGCCGGGCGCCGGTGAAGTATGATCTGGTGGCGTTTACCATCGACTCTGGGTACCCCGGC
TTCCGCAGCGACGTCATCGCTGCCCATCTCAAGGAGCATGGCTTCAGCCACCACGTGGAGGCCACCACCCACTACGACAT
CATCAAGGAAAAGCGGCGCCCCGGCTCCTCCTACTGTTCCATCTGCGCCCGGCTCAAGCGGGGCGTCCTCTACACCCTGG
CTCAGCAGCACGGCTGCAACAAGCTGGCCCTGGGGCACCATCTGGACGACTTCGTGGAGACCCTCCTCCTCAACCAGTTC
TTCGTGGGGACCCTCAAGGCCATGGCGCCGCGGATGCTGGCCGACAACGGCGCGACCACGGTGATCCGTCCCCTGGTCTA
CGTTGAGGAGCGGGAGATCATCCCCTTTGCCCGGGAGAACAGCTTTCCCGTGGTCTGCTGCTGCTGCCCGGTCTGCGGCA
AGGCTGACATCCAGCGCAAGCGGATGAAGGAGCTTCTGAAGGAGTTGGAGCGGGAGAATCCCGCTGTCAAGCGGAGCCTT
CTGCGGGCATTGGCCAACGTGCAGCCGCGGCACCTCCTCGACCGGGAGCTGCAGCGGGTCTGCGAGGTCCCCTGA

Upstream 100 bases:

>100_bases
GCGTTACGGGAATAATAGCAGGACGGTCCCGGGGGAACAAGGGAAAGGGAGGGGCACACTGTCTTGACTTGGGCAAGACC
GGTGGGGTACCGTAGTCGAA

Downstream 100 bases:

>100_bases
CGACAGCCTTTGCCCGGGCCGTCTCCCGCTTGGCGCTGCGGGCCGGTGCCCGGTCCCGGATCATGTCCATGATGCAGATA
TAGGCGCCCCAGGCCACGAG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MWKEKESVEFVDEKRYRKIKNGVGRAVADFNLIEEGDRIAVAVSGGKDSYTLLHILEALRRRAPVKYDLVAFTIDSGYPG
FRSDVIAAHLKEHGFSHHVEATTHYDIIKEKRRPGSSYCSICARLKRGVLYTLAQQHGCNKLALGHHLDDFVETLLLNQF
FVGTLKAMAPRMLADNGATTVIRPLVYVEEREIIPFARENSFPVVCCCCPVCGKADIQRKRMKELLKELERENPAVKRSL
LRALANVQPRHLLDRELQRVCEVP

Sequences:

>Translated_264_residues
MWKEKESVEFVDEKRYRKIKNGVGRAVADFNLIEEGDRIAVAVSGGKDSYTLLHILEALRRRAPVKYDLVAFTIDSGYPG
FRSDVIAAHLKEHGFSHHVEATTHYDIIKEKRRPGSSYCSICARLKRGVLYTLAQQHGCNKLALGHHLDDFVETLLLNQF
FVGTLKAMAPRMLADNGATTVIRPLVYVEEREIIPFARENSFPVVCCCCPVCGKADIQRKRMKELLKELERENPAVKRSL
LRALANVQPRHLLDRELQRVCEVP
>Mature_264_residues
MWKEKESVEFVDEKRYRKIKNGVGRAVADFNLIEEGDRIAVAVSGGKDSYTLLHILEALRRRAPVKYDLVAFTIDSGYPG
FRSDVIAAHLKEHGFSHHVEATTHYDIIKEKRRPGSSYCSICARLKRGVLYTLAQQHGCNKLALGHHLDDFVETLLLNQF
FVGTLKAMAPRMLADNGATTVIRPLVYVEEREIIPFARENSFPVVCCCCPVCGKADIQRKRMKELLKELERENPAVKRSL
LRALANVQPRHLLDRELQRVCEVP

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=245, Percent_Identity=42.0408163265306, Blast_Score=220, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI193211090, Length=245, Percent_Identity=35.5102040816327, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17540090, Length=260, Percent_Identity=27.3076923076923, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24651972, Length=227, Percent_Identity=28.6343612334802, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_GEOMG (Q39QC5)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386274.1
- ProteinModelPortal:   Q39QC5
- STRING:   Q39QC5
- GeneID:   3740651
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3336
- NMPDR:   fig|269799.3.peg.3312
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- PhylomeDB:   Q39QC5
- ProtClustDB:   PRK10696
- BioCyc:   GMET269799:GMET_3336-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 30026; Mature: 30026

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKEKESVEFVDEKRYRKIKNGVGRAVADFNLIEEGDRIAVAVSGGKDSYTLLHILEALR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHH
RRAPVKYDLVAFTIDSGYPGFRSDVIAAHLKEHGFSHHVEATTHYDIIKEKRRPGSSYCS
HHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHH
ICARLKRGVLYTLAQQHGCNKLALGHHLDDFVETLLLNQFFVGTLKAMAPRMLADNGATT
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VIRPLVYVEEREIIPFARENSFPVVCCCCPVCGKADIQRKRMKELLKELERENPAVKRSL
HHHHHHHCCCCCCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
LRALANVQPRHLLDRELQRVCEVP
HHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWKEKESVEFVDEKRYRKIKNGVGRAVADFNLIEEGDRIAVAVSGGKDSYTLLHILEALR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHH
RRAPVKYDLVAFTIDSGYPGFRSDVIAAHLKEHGFSHHVEATTHYDIIKEKRRPGSSYCS
HHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHH
ICARLKRGVLYTLAQQHGCNKLALGHHLDDFVETLLLNQFFVGTLKAMAPRMLADNGATT
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VIRPLVYVEEREIIPFARENSFPVVCCCCPVCGKADIQRKRMKELLKELERENPAVKRSL
HHHHHHHCCCCCCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
LRALANVQPRHLLDRELQRVCEVP
HHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA