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The map label for this gene is zraS [H]

Identifier: 78224470

GI number: 78224470

Start: 3688154

End: 3689980

Strand: Direct

Name: zraS [H]

Synonym: Gmet_3279

Alternate gene names: 78224470

Gene position: 3688154-3689980 (Clockwise)

Preceding gene: 78224469

Following gene: 78224475

Centisome position: 92.26

GC content: 59.17

Gene sequence:

>1827_bases
ATGGCTGCTCAGAATCAATTCAAGATAGCCAGCAATGCGATTCGGATAGCCAACTCCACCGAACACCCTCACCATGTCCG
GCTGAAAGCCTTCGCGCACCTCATTACCGAAGCCTTCCACTGCTCATCGGCGACCATCTACCTGGCCGACGAGAGAGACC
ACCTGCTCCACCGAAAGATATCCACTCTCCTCCCCGACGAAATCAGCGATTGCATTATCCCCTTCGGCAAGGGAGTTGCA
GGACGATGCGCGGCGCATGCCAGACCAATCTACCGCGGAATCTCGTCATTGCACCCCCACGAGCCCTCCCTGGGCACCGA
ACACCAAGTACATGCCCACCCCATCATCGACGGAGAGCGACTGCTGGGGGTCCTGACCATCGGCCTCCCCGACACCACCC
TTCTCGACGGGGAGGATCTCGAACTTCTGCAGGTCCTCTTGATCGAGGCCGCCGGCATTATCCGCCGCAAGCGCTCCCGC
GATGACATGAACAAGCGGCTTGAGGAGTTTTCTTTCCTCTACCGCATCAGCAACGCGATGCTCTCCACCATCAAGCTGAA
CCGACTCGTGCACCTGATTCTTTCAGCCATCACGTCGGGGACCCCCCCTGTTTTCGACCGTGCCATGCTCTTTCTGGCCA
ACGAACGGACCGAGATCCTCCAGGGAATGACCGGCATCGCCCGGGCCGGGGGACCTGAACCGCCGGCGGACCTGCCACCC
GCCCCCACGCCGGATTCCCTGAACGTATCAGACGAAGAGATTGCCCTCCTACAGCAAACCGAATTTTGCAGACTTGTCAA
GGCGACGCGGCTTCCCCTGGACAGCACCCAGAACCACATCTCCAAGGCAGTGCTCGACAGGCAGCTGGTCCTTGTCCGCG
CCCCCCGTCGCGAATCCCCCATTGACCGATCCTTCAACCGGAAGTTCGGCGGTACCCCATTCGCCATCGCCCCACTCATC
GCCCACAATCAGGTCATCGGCGCCATCGTCGTCGACAACTGCACCAGCGGACGCCCCATCACCCCCGATGAACTCAATCT
GCTGCAACTGTTCACCAACCAGGCGGGAATGGCGGCGGAAAACTCCATCCTCTACAACCGGCTCGAAGAAACCAACCGCA
ATCTCCACGAGACCCAGGAGCGCCTCCTGCAGGGGGAGAAACTGGCTGCCATCGGCCAGATGGCCGCCGGAATCGCCCAC
GAGATCAAGAACCCTCTCGTTTCGGTAGGGGGCTTTGCGGGACGGCTGAAAAGGAGATTCCCCGCCGATACCGAGGAATG
GCAATACGCGGATCTCATCACCCGTGAAGTCCAGCATCTGGAAACCATGCTGACCGACATCCTCTTCTTCTCGAAGAAGA
CGACCATCTGCTACAGCCGGTGCAGCATTAACCAGATTATCGAAGACGCCCTCTCGGTGGTCGCTCTCCCCATCGAGGAA
AAAGGAATTAAGGTTGAAAAAATCCTGGCATCGCGCCTCCCCTCGGTCCTGGGCGACTACCAGCAGATCCGCCACGTTTT
CATCAACCTGTTCAGCAACGCCAATGACGTCATGGAGCCTGGGGGCGTCCTGGAAATCGAGTCCCTCCCCGCCCAGATCG
ACGGAAGCCGAGCCGTCTCAGTCAAGGTTTCCGACACAGGAGCAGGGATTCTCCTTGAACATATCCATACCCTCTTCAGC
CCCTTCTTCACCACGAAGGAAAAAGGGACCGGCCTCGGTCTGGCTATCGCCCACAAGATCATAACGGCCCATGGAGGCAC
CATCACGGTTAAAAACCGCGAGACCGGAGGAGCTGAATTCACCGTCGTCATCCCGGTATCCCCCTAA

Upstream 100 bases:

>100_bases
TGACTGCAAAATGGAGCAGGAGCGGAAAGAAAAACACCCGTAATCTTATCCTTTCGTCCCGCTTACTCCCCGTAGCATCT
ACCCGTGCCCCGGAGTCCGG

Downstream 100 bases:

>100_bases
CTTTGCCCTTTTCAAGAGAATCAGTTTACGGTATAAACACGATGTTGTGTTTGTCTAAACAGGAGCATTTACAATTCAAT
AACGGGGATGTAGCTCAGCT

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MAAQNQFKIASNAIRIANSTEHPHHVRLKAFAHLITEAFHCSSATIYLADERDHLLHRKISTLLPDEISDCIIPFGKGVA
GRCAAHARPIYRGISSLHPHEPSLGTEHQVHAHPIIDGERLLGVLTIGLPDTTLLDGEDLELLQVLLIEAAGIIRRKRSR
DDMNKRLEEFSFLYRISNAMLSTIKLNRLVHLILSAITSGTPPVFDRAMLFLANERTEILQGMTGIARAGGPEPPADLPP
APTPDSLNVSDEEIALLQQTEFCRLVKATRLPLDSTQNHISKAVLDRQLVLVRAPRRESPIDRSFNRKFGGTPFAIAPLI
AHNQVIGAIVVDNCTSGRPITPDELNLLQLFTNQAGMAAENSILYNRLEETNRNLHETQERLLQGEKLAAIGQMAAGIAH
EIKNPLVSVGGFAGRLKRRFPADTEEWQYADLITREVQHLETMLTDILFFSKKTTICYSRCSINQIIEDALSVVALPIEE
KGIKVEKILASRLPSVLGDYQQIRHVFINLFSNANDVMEPGGVLEIESLPAQIDGSRAVSVKVSDTGAGILLEHIHTLFS
PFFTTKEKGTGLGLAIAHKIITAHGGTITVKNRETGGAEFTVVIPVSP

Sequences:

>Translated_608_residues
MAAQNQFKIASNAIRIANSTEHPHHVRLKAFAHLITEAFHCSSATIYLADERDHLLHRKISTLLPDEISDCIIPFGKGVA
GRCAAHARPIYRGISSLHPHEPSLGTEHQVHAHPIIDGERLLGVLTIGLPDTTLLDGEDLELLQVLLIEAAGIIRRKRSR
DDMNKRLEEFSFLYRISNAMLSTIKLNRLVHLILSAITSGTPPVFDRAMLFLANERTEILQGMTGIARAGGPEPPADLPP
APTPDSLNVSDEEIALLQQTEFCRLVKATRLPLDSTQNHISKAVLDRQLVLVRAPRRESPIDRSFNRKFGGTPFAIAPLI
AHNQVIGAIVVDNCTSGRPITPDELNLLQLFTNQAGMAAENSILYNRLEETNRNLHETQERLLQGEKLAAIGQMAAGIAH
EIKNPLVSVGGFAGRLKRRFPADTEEWQYADLITREVQHLETMLTDILFFSKKTTICYSRCSINQIIEDALSVVALPIEE
KGIKVEKILASRLPSVLGDYQQIRHVFINLFSNANDVMEPGGVLEIESLPAQIDGSRAVSVKVSDTGAGILLEHIHTLFS
PFFTTKEKGTGLGLAIAHKIITAHGGTITVKNRETGGAEFTVVIPVSP
>Mature_607_residues
AAQNQFKIASNAIRIANSTEHPHHVRLKAFAHLITEAFHCSSATIYLADERDHLLHRKISTLLPDEISDCIIPFGKGVAG
RCAAHARPIYRGISSLHPHEPSLGTEHQVHAHPIIDGERLLGVLTIGLPDTTLLDGEDLELLQVLLIEAAGIIRRKRSRD
DMNKRLEEFSFLYRISNAMLSTIKLNRLVHLILSAITSGTPPVFDRAMLFLANERTEILQGMTGIARAGGPEPPADLPPA
PTPDSLNVSDEEIALLQQTEFCRLVKATRLPLDSTQNHISKAVLDRQLVLVRAPRRESPIDRSFNRKFGGTPFAIAPLIA
HNQVIGAIVVDNCTSGRPITPDELNLLQLFTNQAGMAAENSILYNRLEETNRNLHETQERLLQGEKLAAIGQMAAGIAHE
IKNPLVSVGGFAGRLKRRFPADTEEWQYADLITREVQHLETMLTDILFFSKKTTICYSRCSINQIIEDALSVVALPIEEK
GIKVEKILASRLPSVLGDYQQIRHVFINLFSNANDVMEPGGVLEIESLPAQIDGSRAVSVKVSDTGAGILLEHIHTLFSP
FFTTKEKGTGLGLAIAHKIITAHGGTITVKNRETGGAEFTVVIPVSP

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=251, Percent_Identity=34.2629482071713, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=31.8965517241379, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1790300, Length=313, Percent_Identity=27.1565495207668, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081816, Length=220, Percent_Identity=28.6363636363636, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=27.734375, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1786912, Length=302, Percent_Identity=26.4900662251656, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=282, Percent_Identity=24.822695035461, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788393, Length=426, Percent_Identity=22.7699530516432, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787894, Length=230, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI48994928, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790346, Length=240, Percent_Identity=25, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 66804; Mature: 66673

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQNQFKIASNAIRIANSTEHPHHVRLKAFAHLITEAFHCSSATIYLADERDHLLHRKI
CCCCCCEEHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHH
STLLPDEISDCIIPFGKGVAGRCAAHARPIYRGISSLHPHEPSLGTEHQVHAHPIIDGER
HHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCC
LLGVLTIGLPDTTLLDGEDLELLQVLLIEAAGIIRRKRSRDDMNKRLEEFSFLYRISNAM
EEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSTIKLNRLVHLILSAITSGTPPVFDRAMLFLANERTEILQGMTGIARAGGPEPPADLPP
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
APTPDSLNVSDEEIALLQQTEFCRLVKATRLPLDSTQNHISKAVLDRQLVLVRAPRRESP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEECCCCCCC
IDRSFNRKFGGTPFAIAPLIAHNQVIGAIVVDNCTSGRPITPDELNLLQLFTNQAGMAAE
CCHHHCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHH
NSILYNRLEETNRNLHETQERLLQGEKLAAIGQMAAGIAHEIKNPLVSVGGFAGRLKRRF
HHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHCC
PADTEEWQYADLITREVQHLETMLTDILFFSKKTTICYSRCSINQIIEDALSVVALPIEE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHEECCCHH
KGIKVEKILASRLPSVLGDYQQIRHVFINLFSNANDVMEPGGVLEIESLPAQIDGSRAVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEECCCCCCCCCCEEEE
VKVSDTGAGILLEHIHTLFSPFFTTKEKGTGLGLAIAHKIITAHGGTITVKNRETGGAEF
EEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEE
TVVIPVSP
EEEEECCC
>Mature Secondary Structure 
AAQNQFKIASNAIRIANSTEHPHHVRLKAFAHLITEAFHCSSATIYLADERDHLLHRKI
CCCCCEEHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHH
STLLPDEISDCIIPFGKGVAGRCAAHARPIYRGISSLHPHEPSLGTEHQVHAHPIIDGER
HHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCC
LLGVLTIGLPDTTLLDGEDLELLQVLLIEAAGIIRRKRSRDDMNKRLEEFSFLYRISNAM
EEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSTIKLNRLVHLILSAITSGTPPVFDRAMLFLANERTEILQGMTGIARAGGPEPPADLPP
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
APTPDSLNVSDEEIALLQQTEFCRLVKATRLPLDSTQNHISKAVLDRQLVLVRAPRRESP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEECCCCCCC
IDRSFNRKFGGTPFAIAPLIAHNQVIGAIVVDNCTSGRPITPDELNLLQLFTNQAGMAAE
CCHHHCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHH
NSILYNRLEETNRNLHETQERLLQGEKLAAIGQMAAGIAHEIKNPLVSVGGFAGRLKRRF
HHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHCC
PADTEEWQYADLITREVQHLETMLTDILFFSKKTTICYSRCSINQIIEDALSVVALPIEE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHEECCCHH
KGIKVEKILASRLPSVLGDYQQIRHVFINLFSNANDVMEPGGVLEIESLPAQIDGSRAVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEECCCCCCCCCCEEEE
VKVSDTGAGILLEHIHTLFSPFFTTKEKGTGLGLAIAHKIITAHGGTITVKNRETGGAEF
EEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEE
TVVIPVSP
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]