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The map label for this gene is radA [H]

Identifier: 78224468

GI number: 78224468

Start: 3686289

End: 3687638

Strand: Direct

Name: radA [H]

Synonym: Gmet_3277

Alternate gene names: 78224468

Gene position: 3686289-3687638 (Clockwise)

Preceding gene: 78224465

Following gene: 78224469

Centisome position: 92.22

GC content: 67.11

Gene sequence:

>1350_bases
TTGAAAACCATGACATTGTATGTCTGCCAGAACTGCGGATACCAGTCGGCCAAGTGGCTCGGTAAGTGCCCCGACTGCTC
CGCCTGGAACAGCCTCGTGGAAGAGGTGCGGGGCAAGGGGAAAGGCCCCTCCGCCCCCCTCTCCGGCGTGGCGCCGGTCC
CCATCTGCGACGTGGCGGGGGAGGCGGAAGAACGGCTCCCCTGCGGCATATCCGAGTTCGACCGGGTCCTGGGCGGAGGG
TTAGTGGCCGGCTCCGTCATCCTTATCGGCGGCGATCCCGGCATCGGAAAATCAACCCTTCTCTTGCAAGTGATGGACCA
CTTGGCGCGCACCGTCGGACCGGTCCTCTACGTTTCCGGCGAAGAGTCGGCCCGGCAGGCGAGGCTGAGGGGAGAGCGGC
TCGGTATCCGGGCAAAGGAGCTTCTCATCCTGGCCGAAACTGCCCTCGAAACAGTGCTGGCCCAGATCGGCGCCGTGGCC
CCCCGGGCCGTGGTCATCGACTCCATCCAGACCATGTACGCCCCGGGGCTCGAATCGGCCCCCGGAAGCGTGAGCCAGGT
GCGGGAGTGCGCGGGGCGCCTCATGGTGGCGGCGAAACGGAGCGCCATCCCCACCTTCATTGTAGGGCACGTCACCAAGG
ACGGCTCCATCGCCGGCCCCCGGGTCCTGGAGCACATGGTCGACACGGTCCTCTACTTCGAGGGGGACCGGGGGCACGCC
TTCCGGATCCTGCGGGCGGTCAAAAACCGCTTCGGCTCCACCAACGAGATCGGCGTTTTCGAGATGAAGGAGGGGGGACT
CTGCGAGGTCCGCAACCCCTCGGAAATATTCCTTTCGGAGCGCCCCCTGGGGGTGTCCGGCTCCGCCGTGGTCACCACCA
TCGAGGGAAGCCGTCCGCTCCTGGTGGAGCTCCAGGCCCTGGTCACCCCTTCCTCCCTGGGGGTTCCCCGCCGCACCACC
CTGGGGGTCGACGCCAACCGTCTTGCCCTCCTGGTGGCCGTCCTCGACAAGAAGGTGGGACTCCACATGGCGGGGCAGGA
CATCTTCCTGAATGCCGCCGGCGGGGCGAGGGTAAGCGAACCGGCCGCCGACCTCGGTATGGTGGCCGCCCTCGCCTCCA
GCCATCTGGACAAGGTCATCGATCCCCAGACGGTGGTTGTGGGCGAGGTGGGACTGGCCGGAGAGGTGCGGGCCATAACC
CAGCCGGAAACCCGGGTACGGGAGGCGGCCAAGCTCGGCTTTACCCGGGCCGTCATCCCCGCCGGCAGCCTCAAGCAGGT
GACCGCCGTGGAGGGGATGGAAGTAATCGGGGTCAAATCGGTTGAAGAGGCCCTGGACCGGCTCCTGTGA

Upstream 100 bases:

>100_bases
CTGACACGGGTTGTCCTGTGGCTCCGTTGACAGACACTCCGAATCGGTTTAGAACTGAGCATTGCGCCACCCCTCGAACC
CGTTGCGGAGGATAATCCGT

Downstream 100 bases:

>100_bases
GCCTCTCCCCATTACCGGCGTCACATACTTTTTACTTTACTTGACCGCACAATCCCTTTATTATCTGTACGATTAAGCGT
CATAAAGGGGAGACACGCGG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKTMTLYVCQNCGYQSAKWLGKCPDCSAWNSLVEEVRGKGKGPSAPLSGVAPVPICDVAGEAEERLPCGISEFDRVLGGG
LVAGSVILIGGDPGIGKSTLLLQVMDHLARTVGPVLYVSGEESARQARLRGERLGIRAKELLILAETALETVLAQIGAVA
PRAVVIDSIQTMYAPGLESAPGSVSQVRECAGRLMVAAKRSAIPTFIVGHVTKDGSIAGPRVLEHMVDTVLYFEGDRGHA
FRILRAVKNRFGSTNEIGVFEMKEGGLCEVRNPSEIFLSERPLGVSGSAVVTTIEGSRPLLVELQALVTPSSLGVPRRTT
LGVDANRLALLVAVLDKKVGLHMAGQDIFLNAAGGARVSEPAADLGMVAALASSHLDKVIDPQTVVVGEVGLAGEVRAIT
QPETRVREAAKLGFTRAVIPAGSLKQVTAVEGMEVIGVKSVEEALDRLL

Sequences:

>Translated_449_residues
MKTMTLYVCQNCGYQSAKWLGKCPDCSAWNSLVEEVRGKGKGPSAPLSGVAPVPICDVAGEAEERLPCGISEFDRVLGGG
LVAGSVILIGGDPGIGKSTLLLQVMDHLARTVGPVLYVSGEESARQARLRGERLGIRAKELLILAETALETVLAQIGAVA
PRAVVIDSIQTMYAPGLESAPGSVSQVRECAGRLMVAAKRSAIPTFIVGHVTKDGSIAGPRVLEHMVDTVLYFEGDRGHA
FRILRAVKNRFGSTNEIGVFEMKEGGLCEVRNPSEIFLSERPLGVSGSAVVTTIEGSRPLLVELQALVTPSSLGVPRRTT
LGVDANRLALLVAVLDKKVGLHMAGQDIFLNAAGGARVSEPAADLGMVAALASSHLDKVIDPQTVVVGEVGLAGEVRAIT
QPETRVREAAKLGFTRAVIPAGSLKQVTAVEGMEVIGVKSVEEALDRLL
>Mature_449_residues
MKTMTLYVCQNCGYQSAKWLGKCPDCSAWNSLVEEVRGKGKGPSAPLSGVAPVPICDVAGEAEERLPCGISEFDRVLGGG
LVAGSVILIGGDPGIGKSTLLLQVMDHLARTVGPVLYVSGEESARQARLRGERLGIRAKELLILAETALETVLAQIGAVA
PRAVVIDSIQTMYAPGLESAPGSVSQVRECAGRLMVAAKRSAIPTFIVGHVTKDGSIAGPRVLEHMVDTVLYFEGDRGHA
FRILRAVKNRFGSTNEIGVFEMKEGGLCEVRNPSEIFLSERPLGVSGSAVVTTIEGSRPLLVELQALVTPSSLGVPRRTT
LGVDANRLALLVAVLDKKVGLHMAGQDIFLNAAGGARVSEPAADLGMVAALASSHLDKVIDPQTVVVGEVGLAGEVRAIT
QPETRVREAAKLGFTRAVIPAGSLKQVTAVEGMEVIGVKSVEEALDRLL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=448, Percent_Identity=47.7678571428571, Blast_Score=396, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 47132; Mature: 47132

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMTLYVCQNCGYQSAKWLGKCPDCSAWNSLVEEVRGKGKGPSAPLSGVAPVPICDVAG
CCEEEEEEECCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCC
EAEERLPCGISEFDRVLGGGLVAGSVILIGGDPGIGKSTLLLQVMDHLARTVGPVLYVSG
CCHHCCCCCHHHHHHHHCCCEEECEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEECC
EESARQARLRGERLGIRAKELLILAETALETVLAQIGAVAPRAVVIDSIQTMYAPGLESA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
PGSVSQVRECAGRLMVAAKRSAIPTFIVGHVTKDGSIAGPRVLEHMVDTVLYFEGDRGHA
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHEEEECCCCHH
FRILRAVKNRFGSTNEIGVFEMKEGGLCEVRNPSEIFLSERPLGVSGSAVVTTIEGSRPL
HHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCHHHEEECCCCCCCCCEEEEEECCCCCE
LVELQALVTPSSLGVPRRTTLGVDANRLALLVAVLDKKVGLHMAGQDIFLNAAGGARVSE
EEEHHHHCCCHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCC
PAADLGMVAALASSHLDKVIDPQTVVVGEVGLAGEVRAITQPETRVREAAKLGFTRAVIP
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCHHHHHHHHHHCCCEEECC
AGSLKQVTAVEGMEVIGVKSVEEALDRLL
CCCCHHHHHHCCCCEECHHHHHHHHHHCC
>Mature Secondary Structure
MKTMTLYVCQNCGYQSAKWLGKCPDCSAWNSLVEEVRGKGKGPSAPLSGVAPVPICDVAG
CCEEEEEEECCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCC
EAEERLPCGISEFDRVLGGGLVAGSVILIGGDPGIGKSTLLLQVMDHLARTVGPVLYVSG
CCHHCCCCCHHHHHHHHCCCEEECEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEECC
EESARQARLRGERLGIRAKELLILAETALETVLAQIGAVAPRAVVIDSIQTMYAPGLESA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
PGSVSQVRECAGRLMVAAKRSAIPTFIVGHVTKDGSIAGPRVLEHMVDTVLYFEGDRGHA
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHEEEECCCCHH
FRILRAVKNRFGSTNEIGVFEMKEGGLCEVRNPSEIFLSERPLGVSGSAVVTTIEGSRPL
HHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCHHHEEECCCCCCCCCEEEEEECCCCCE
LVELQALVTPSSLGVPRRTTLGVDANRLALLVAVLDKKVGLHMAGQDIFLNAAGGARVSE
EEEHHHHCCCHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCC
PAADLGMVAALASSHLDKVIDPQTVVVGEVGLAGEVRAITQPETRVREAAKLGFTRAVIP
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCHHHHHHHHHHCCCEEECC
AGSLKQVTAVEGMEVIGVKSVEEALDRLL
CCCCHHHHHHCCCCEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]