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The map label for this gene is yjbJ [H]

Identifier: 78224453

GI number: 78224453

Start: 3671554

End: 3672150

Strand: Reverse

Name: yjbJ [H]

Synonym: Gmet_3262

Alternate gene names: 78224453

Gene position: 3672150-3671554 (Counterclockwise)

Preceding gene: 78224454

Following gene: 78224452

Centisome position: 91.86

GC content: 61.14

Gene sequence:

>597_bases
ATGCGGCTCGACATGATGCGGAGCGCCTTCACTCTTGGCGACTCCGAAGGTGGGGGGAATCCGCCGGCAATGGGGCGTGC
CGTGGAATTCATGCTCAAGAGCTTTGCCGAAAACAGCCGGGAACCTGTTGATCCGGCATCTCCCGTGGCTGCCGGGGGAT
CTTCTGCTGCCCCGGTTGCTTCTGCCGCTCCCGAGCCTCTGCCCGCAAAGGGCAGTGGCAACTGGCTCGACGACGTCGTC
AATCGTGCTTCACGCCGCCATGGCGTCGAGGTTGGACTCATCAAGGCAGTCATCAAGGCCGAAAGCAACTTCAATCCCAA
CGCCGTCTCCCCGGTGGGCGCCCAGGGGCTCATGCAGCTCATGCCTGCCACCGCCAAGGGACTCGGGGTCACCAACTCCT
TCGATCCCGAGCAGAATGTCATGGCCGGCACAAAGTTTCTCAAGGATCTCCTGGCCCGTTACGGCGGGAATGTGGACAAG
GCCCTTGCCGCCTACAATTGGGGGCCGGGCAATGTCGATCGCAAGCCCCATCTCCTTCCCCGGGAAACCCGCGAATATCT
TGCCAAGGTGAAAGATTACTACAATCAGTACGCCTGA

Upstream 100 bases:

>100_bases
GGCCGGTGCAGGCCTCGTCTTCGACGAGTTCCTGGCTGCCGGTACCGGTATCGACGTGCAGCCGGGAGCACGTGCCTCCG
CTGCCGCTGCTGCGGAATTG

Downstream 100 bases:

>100_bases
TGGCGCCACTTGATCCCCGTCGGTCGTCAACGGCCCTTTCGCGTGATGCGAGGGGGCCTTTTTCGTGGTAAACATTCTCC
GTAGCGCAGCATCGCTTTTG

Product: lytic transglycosylase, catalytic

Products: N-Acetylmuramic Acid Residues; N-Acetylglucosamine Residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MRLDMMRSAFTLGDSEGGGNPPAMGRAVEFMLKSFAENSREPVDPASPVAAGGSSAAPVASAAPEPLPAKGSGNWLDDVV
NRASRRHGVEVGLIKAVIKAESNFNPNAVSPVGAQGLMQLMPATAKGLGVTNSFDPEQNVMAGTKFLKDLLARYGGNVDK
ALAAYNWGPGNVDRKPHLLPRETREYLAKVKDYYNQYA

Sequences:

>Translated_198_residues
MRLDMMRSAFTLGDSEGGGNPPAMGRAVEFMLKSFAENSREPVDPASPVAAGGSSAAPVASAAPEPLPAKGSGNWLDDVV
NRASRRHGVEVGLIKAVIKAESNFNPNAVSPVGAQGLMQLMPATAKGLGVTNSFDPEQNVMAGTKFLKDLLARYGGNVDK
ALAAYNWGPGNVDRKPHLLPRETREYLAKVKDYYNQYA
>Mature_198_residues
MRLDMMRSAFTLGDSEGGGNPPAMGRAVEFMLKSFAENSREPVDPASPVAAGGSSAAPVASAAPEPLPAKGSGNWLDDVV
NRASRRHGVEVGLIKAVIKAESNFNPNAVSPVGAQGLMQLMPATAKGLGVTNSFDPEQNVMAGTKFLKDLLARYGGNVDK
ALAAYNWGPGNVDRKPHLLPRETREYLAKVKDYYNQYA

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082191, Length=115, Percent_Identity=39.1304347826087, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI87082441, Length=106, Percent_Identity=35.8490566037736, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 21017; Mature: 21017

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDMMRSAFTLGDSEGGGNPPAMGRAVEFMLKSFAENSREPVDPASPVAAGGSSAAPVA
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SAAPEPLPAKGSGNWLDDVVNRASRRHGVEVGLIKAVIKAESNFNPNAVSPVGAQGLMQL
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
MPATAKGLGVTNSFDPEQNVMAGTKFLKDLLARYGGNVDKALAAYNWGPGNVDRKPHLLP
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCC
RETREYLAKVKDYYNQYA
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLDMMRSAFTLGDSEGGGNPPAMGRAVEFMLKSFAENSREPVDPASPVAAGGSSAAPVA
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SAAPEPLPAKGSGNWLDDVVNRASRRHGVEVGLIKAVIKAESNFNPNAVSPVGAQGLMQL
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
MPATAKGLGVTNSFDPEQNVMAGTKFLKDLLARYGGNVDKALAAYNWGPGNVDRKPHLLP
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCC
RETREYLAKVKDYYNQYA
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]