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The map label for this gene is zraR [H]

Identifier: 78224454

GI number: 78224454

Start: 3672337

End: 3673731

Strand: Reverse

Name: zraR [H]

Synonym: Gmet_3263

Alternate gene names: 78224454

Gene position: 3673731-3672337 (Counterclockwise)

Preceding gene: 78224461

Following gene: 78224453

Centisome position: 91.9

GC content: 58.14

Gene sequence:

>1395_bases
ATGGATACGCCGCGACTGCTCATTGCCGACGACGACAAAAAAACCCGAGACTTTGTAGCCGCTTTTCTCAAGTACAAAGG
GTACGACGTGGTCCAGGCCTGTGACGGTCAGGACGCCCTCGACAAGCTGGAAACCGAAGAGGTTCACCTTGTCATCACCG
ACCTGATGATGCCCCGGGTGAACGGCCTCGAATTCGTCAAGAAGCTGAAGACCATGCGTCCGGGCACCGTCATCATTGCC
TACAGCGCCTTCGGCAACTACGAAATGGCCTCCAACCTCCTCAAGGCAGGGGTCTTTTTCTATCTGGAAAAGCCCTTCAA
TCTGGACGAGCTTGAAACGCACGTGAAGCGCGGGTTCGAGCACCAGTCCCTCCAGAGCCAGACCTACCGGGCCAAGCCGG
CCCTCAAGAACCGGGCCCTCATGCCGAATATCATCGGCGAGAGCTCCAAGATGCTCTCCCTCTTCGAGCTGATCGAAAAG
GTTGCCGAGTCCGATTCCACCGTCCTCATTCAGGGTGAGTCGGGGACCGGCAAGGAACTGGTCGCCCGGGCGGTCCATGA
CCTGAGCAATCGCAAGACTCGCAACTTCGTGCCGGTCAACTGCGCCGCCATCCCCGACGAGCTCCTTGAAAGCGAGCTCT
TCGGCCACGTGAAAGGTTCCTTCACCGGCGCCGTGGCGAACCGCATCGGCCGTTTCGAGATGGCCGACCGCGGCACCCTG
TTTCTCGACGAAATCGGCGACATGAAACCGAACCTCCAGGTGAAGCTCTTGCGGGTGCTGCAGAACCGCGAGCTGGAGCC
GGTGGGTGCCACCCGCTCCAAGAAGATCGACGTGCGGATCATTGCCGCCACCAACCAGAACCTGGAAAAGCTGGTCGCCA
CGAAACAGTTCCGCGAAGACCTCTACTACCGGCTCTCTGTCATTCCCATCTTCATTCCGCCCCTGCGGGAGCGCAAGGAA
GATATCCCGCTCCTGGTCAACTCTTTCCTTGAGCGTTTCAACCGCAACAAGAAGAGCAAGGTGAAGGGGATCGACTCCGA
CGCCATGGACCTTCTCGTCCACTACGACTGGCCCGGCAACGTGCGCGAACTGGAGAACCTCGTGGAGCGCCTCGTGATCC
TCAAGGGGTTCGGCGCCATTGGTGTCAATGATCTGCCCGAAAAGTTCTCCGGCGTCACCATTTCCCCCCCTTCCGAAAGC
ATGACCCTTCCCGATTCGGGCATCTGCCTCAATACCGTGGTGGAGGAGTTCGAAAACAACCTCATCCTCCAGGCCCTCAA
GAAGACCGGCGGCAACAAGAAGGAGGCCGCTCTTCTTCTGAACCTCAAGCGAACCACCCTCATCGAGAAATTAAAAAAGC
GGAGACTCGATACAATTGCCACGTCTTTTGCATAA

Upstream 100 bases:

>100_bases
TCACTGGTCGTCATTTTGTTGACTTTTGTCAGGATTATGACTACACTGACGTCCGCATCGAGATGACCCGCTCCAGATAC
CCAGTTTGTTCGAGGAAACC

Downstream 100 bases:

>100_bases
GACGTGGGTATGATCATCGATACTCTTATCAATCGTTCCACTCCATCCGAATCCAAACCCCAGACTGACCGCCCCACTGT
TCGAGAGGCCGGTGCAGGCC

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MDTPRLLIADDDKKTRDFVAAFLKYKGYDVVQACDGQDALDKLETEEVHLVITDLMMPRVNGLEFVKKLKTMRPGTVIIA
YSAFGNYEMASNLLKAGVFFYLEKPFNLDELETHVKRGFEHQSLQSQTYRAKPALKNRALMPNIIGESSKMLSLFELIEK
VAESDSTVLIQGESGTGKELVARAVHDLSNRKTRNFVPVNCAAIPDELLESELFGHVKGSFTGAVANRIGRFEMADRGTL
FLDEIGDMKPNLQVKLLRVLQNRELEPVGATRSKKIDVRIIAATNQNLEKLVATKQFREDLYYRLSVIPIFIPPLRERKE
DIPLLVNSFLERFNRNKKSKVKGIDSDAMDLLVHYDWPGNVRELENLVERLVILKGFGAIGVNDLPEKFSGVTISPPSES
MTLPDSGICLNTVVEEFENNLILQALKKTGGNKKEAALLLNLKRTTLIEKLKKRRLDTIATSFA

Sequences:

>Translated_464_residues
MDTPRLLIADDDKKTRDFVAAFLKYKGYDVVQACDGQDALDKLETEEVHLVITDLMMPRVNGLEFVKKLKTMRPGTVIIA
YSAFGNYEMASNLLKAGVFFYLEKPFNLDELETHVKRGFEHQSLQSQTYRAKPALKNRALMPNIIGESSKMLSLFELIEK
VAESDSTVLIQGESGTGKELVARAVHDLSNRKTRNFVPVNCAAIPDELLESELFGHVKGSFTGAVANRIGRFEMADRGTL
FLDEIGDMKPNLQVKLLRVLQNRELEPVGATRSKKIDVRIIAATNQNLEKLVATKQFREDLYYRLSVIPIFIPPLRERKE
DIPLLVNSFLERFNRNKKSKVKGIDSDAMDLLVHYDWPGNVRELENLVERLVILKGFGAIGVNDLPEKFSGVTISPPSES
MTLPDSGICLNTVVEEFENNLILQALKKTGGNKKEAALLLNLKRTTLIEKLKKRRLDTIATSFA
>Mature_464_residues
MDTPRLLIADDDKKTRDFVAAFLKYKGYDVVQACDGQDALDKLETEEVHLVITDLMMPRVNGLEFVKKLKTMRPGTVIIA
YSAFGNYEMASNLLKAGVFFYLEKPFNLDELETHVKRGFEHQSLQSQTYRAKPALKNRALMPNIIGESSKMLSLFELIEK
VAESDSTVLIQGESGTGKELVARAVHDLSNRKTRNFVPVNCAAIPDELLESELFGHVKGSFTGAVANRIGRFEMADRGTL
FLDEIGDMKPNLQVKLLRVLQNRELEPVGATRSKKIDVRIIAATNQNLEKLVATKQFREDLYYRLSVIPIFIPPLRERKE
DIPLLVNSFLERFNRNKKSKVKGIDSDAMDLLVHYDWPGNVRELENLVERLVILKGFGAIGVNDLPEKFSGVTISPPSES
MTLPDSGICLNTVVEEFENNLILQALKKTGGNKKEAALLLNLKRTTLIEKLKKRRLDTIATSFA

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=453, Percent_Identity=43.9293598233996, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788550, Length=453, Percent_Identity=40.6181015452539, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1788905, Length=456, Percent_Identity=41.2280701754386, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1790299, Length=465, Percent_Identity=39.7849462365591, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1789087, Length=320, Percent_Identity=49.375, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI87082117, Length=338, Percent_Identity=45.2662721893491, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1789233, Length=345, Percent_Identity=46.6666666666667, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=46.3722397476341, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI1786524, Length=349, Percent_Identity=38.9684813753582, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=39.8148148148148, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=34.8909657320872, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081858, Length=338, Percent_Identity=34.9112426035503, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1789828, Length=240, Percent_Identity=35.8333333333333, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1788713, Length=112, Percent_Identity=34.8214285714286, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1786784, Length=112, Percent_Identity=33.9285714285714, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52213; Mature: 52213

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPRLLIADDDKKTRDFVAAFLKYKGYDVVQACDGQDALDKLETEEVHLVITDLMMPRV
CCCCCEEEECCCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHCCCCHHEEEHHHHHCCCC
NGLEFVKKLKTMRPGTVIIAYSAFGNYEMASNLLKAGVFFYLEKPFNLDELETHVKRGFE
CHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCCC
HQSLQSQTYRAKPALKNRALMPNIIGESSKMLSLFELIEKVAESDSTVLIQGESGTGKEL
HHHHHHHHHHCCCHHHCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
VARAVHDLSNRKTRNFVPVNCAAIPDELLESELFGHVKGSFTGAVANRIGRFEMADRGTL
HHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEECCCCCE
FLDEIGDMKPNLQVKLLRVLQNRELEPVGATRSKKIDVRIIAATNQNLEKLVATKQFRED
EHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHH
LYYRLSVIPIFIPPLRERKEDIPLLVNSFLERFNRNKKSKVKGIDSDAMDLLVHYDWPGN
HHHHHEEEEEECCCHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCCC
VRELENLVERLVILKGFGAIGVNDLPEKFSGVTISPPSESMTLPDSGICLNTVVEEFENN
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LILQALKKTGGNKKEAALLLNLKRTTLIEKLKKRRLDTIATSFA
HHHHHHHHCCCCCCHHEEEEECHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTPRLLIADDDKKTRDFVAAFLKYKGYDVVQACDGQDALDKLETEEVHLVITDLMMPRV
CCCCCEEEECCCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHCCCCHHEEEHHHHHCCCC
NGLEFVKKLKTMRPGTVIIAYSAFGNYEMASNLLKAGVFFYLEKPFNLDELETHVKRGFE
CHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCCC
HQSLQSQTYRAKPALKNRALMPNIIGESSKMLSLFELIEKVAESDSTVLIQGESGTGKEL
HHHHHHHHHHCCCHHHCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
VARAVHDLSNRKTRNFVPVNCAAIPDELLESELFGHVKGSFTGAVANRIGRFEMADRGTL
HHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEECCCCCE
FLDEIGDMKPNLQVKLLRVLQNRELEPVGATRSKKIDVRIIAATNQNLEKLVATKQFRED
EHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHH
LYYRLSVIPIFIPPLRERKEDIPLLVNSFLERFNRNKKSKVKGIDSDAMDLLVHYDWPGN
HHHHHEEEEEECCCHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCCC
VRELENLVERLVILKGFGAIGVNDLPEKFSGVTISPPSESMTLPDSGICLNTVVEEFENN
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LILQALKKTGGNKKEAALLLNLKRTTLIEKLKKRRLDTIATSFA
HHHHHHHHCCCCCCHHEEEEECHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]