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The map label for this gene is yagI [H]

Identifier: 78224220

GI number: 78224220

Start: 3412703

End: 3413485

Strand: Reverse

Name: yagI [H]

Synonym: Gmet_3027

Alternate gene names: 78224220

Gene position: 3413485-3412703 (Counterclockwise)

Preceding gene: 78224222

Following gene: 78224216

Centisome position: 85.39

GC content: 57.09

Gene sequence:

>783_bases
ATGGCCAAGAAGGATAAATCGGAATATATCATTCAGGCGGTCGATCACGCCCTTGACCTTCTTGAGCAGTTTCATGAAGA
TGTGGACGAACTCGGCGTTACGGAGCTCTCCAAGCGGCTGAAACTCCACAAGAATAACGTGTTCCGGCTCCTGGCCACCC
TGGAGTCGCGGGGCTATATCGAGCAGAACAGGGTTACGGAGAACTACCGGCTCGGGCTCAAGACTCTGGAACTGGGGCAG
ACCTTCATCAAGCAGATGGGACTTCTGCGCCAGTCGCGGCCGGTGCTCGAGTGGCTCGTCAAGGAGTGCAACGAGACCGC
CTACGTGGCGATTCTCAAGGAGCAGAGCATTGTCTATCTGGATGTGGTGGAGACCGACCTCACCGTGCGGGTTGTACCCC
GTGTCGGCTCCCGGCTTCCGGCATACTGCACCGCAGCGGGAAAGGTTCAGATTGCATACATGCCCGACGAAGAACTTGAT
GCCTTTTTCCCTGTCAAAGAGCTCAAGCCGTACACTCCCAATACGATCACCGACCGTGACGTGCTGAGAAAGCACCTGAT
GGAAGTGGCGGAGCAGGGGTACGCCATCGACAACGAGGAACTCGATCTGGGGGTCCGCTGTGCCGGCGCGCCGATCCGTG
ACTATACCCGCCGGATCATCGGCGCGGTCAGTCTTTCCGGGCCATCCATGCGTTTTACCGACGAGAGGATGGCAAAGGAA
CTCATTCCCCTCATTACGCAGGCCGCCGATGAGATTTCCATGAAACTCGGCTACCACAAATAA

Upstream 100 bases:

>100_bases
CAATGGAGTGACGGTTTCTGGCAGAGACGTTACCCTTGACTTATTTCCCCTTAAAGCTTATGTAAGAAGGCTTATTTCTT
TCTGTGAAAGAGGAGGGCCA

Downstream 100 bases:

>100_bases
AAAAACCCCCGGATTTACCGGGGGTTTTTTTCTATTCTCTCCTTCCAAAGGGCAACCACCTGCCGTTCCGTCTCCTCTGA
CGTCCCCCGGTTGTCAATGA

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MAKKDKSEYIIQAVDHALDLLEQFHEDVDELGVTELSKRLKLHKNNVFRLLATLESRGYIEQNRVTENYRLGLKTLELGQ
TFIKQMGLLRQSRPVLEWLVKECNETAYVAILKEQSIVYLDVVETDLTVRVVPRVGSRLPAYCTAAGKVQIAYMPDEELD
AFFPVKELKPYTPNTITDRDVLRKHLMEVAEQGYAIDNEELDLGVRCAGAPIRDYTRRIIGAVSLSGPSMRFTDERMAKE
LIPLITQAADEISMKLGYHK

Sequences:

>Translated_260_residues
MAKKDKSEYIIQAVDHALDLLEQFHEDVDELGVTELSKRLKLHKNNVFRLLATLESRGYIEQNRVTENYRLGLKTLELGQ
TFIKQMGLLRQSRPVLEWLVKECNETAYVAILKEQSIVYLDVVETDLTVRVVPRVGSRLPAYCTAAGKVQIAYMPDEELD
AFFPVKELKPYTPNTITDRDVLRKHLMEVAEQGYAIDNEELDLGVRCAGAPIRDYTRRIIGAVSLSGPSMRFTDERMAKE
LIPLITQAADEISMKLGYHK
>Mature_259_residues
AKKDKSEYIIQAVDHALDLLEQFHEDVDELGVTELSKRLKLHKNNVFRLLATLESRGYIEQNRVTENYRLGLKTLELGQT
FIKQMGLLRQSRPVLEWLVKECNETAYVAILKEQSIVYLDVVETDLTVRVVPRVGSRLPAYCTAAGKVQIAYMPDEELDA
FFPVKELKPYTPNTITDRDVLRKHLMEVAEQGYAIDNEELDLGVRCAGAPIRDYTRRIIGAVSLSGPSMRFTDERMAKEL
IPLITQAADEISMKLGYHK

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=249, Percent_Identity=36.144578313253, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1788131, Length=258, Percent_Identity=35.2713178294574, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI87082359, Length=249, Percent_Identity=29.718875502008, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1786715, Length=246, Percent_Identity=29.6747967479675, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789999, Length=261, Percent_Identity=28.3524904214559, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790752, Length=225, Percent_Identity=28.8888888888889, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788581, Length=225, Percent_Identity=24.4444444444444, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29658; Mature: 29526

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKDKSEYIIQAVDHALDLLEQFHEDVDELGVTELSKRLKLHKNNVFRLLATLESRGYI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EQNRVTENYRLGLKTLELGQTFIKQMGLLRQSRPVLEWLVKECNETAYVAILKEQSIVYL
HHHCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCEEEE
DVVETDLTVRVVPRVGSRLPAYCTAAGKVQIAYMPDEELDAFFPVKELKPYTPNTITDRD
EEECCCCEEEEECHHCCCCCCCEECCCCEEEEECCCHHHHHHCCHHHCCCCCCCCCCCHH
VLRKHLMEVAEQGYAIDNEELDLGVRCAGAPIRDYTRRIIGAVSLSGPSMRFTDERMAKE
HHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LIPLITQAADEISMKLGYHK
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKKDKSEYIIQAVDHALDLLEQFHEDVDELGVTELSKRLKLHKNNVFRLLATLESRGYI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EQNRVTENYRLGLKTLELGQTFIKQMGLLRQSRPVLEWLVKECNETAYVAILKEQSIVYL
HHHCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCEEEE
DVVETDLTVRVVPRVGSRLPAYCTAAGKVQIAYMPDEELDAFFPVKELKPYTPNTITDRD
EEECCCCEEEEECHHCCCCCCCEECCCCEEEEECCCHHHHHHCCHHHCCCCCCCCCCCHH
VLRKHLMEVAEQGYAIDNEELDLGVRCAGAPIRDYTRRIIGAVSLSGPSMRFTDERMAKE
HHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LIPLITQAADEISMKLGYHK
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]