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The map label for this gene is rpfG [H]

Identifier: 78224192

GI number: 78224192

Start: 3384504

End: 3385589

Strand: Direct

Name: rpfG [H]

Synonym: Gmet_2999

Alternate gene names: 78224192

Gene position: 3384504-3385589 (Clockwise)

Preceding gene: 78224191

Following gene: 78224199

Centisome position: 84.67

GC content: 56.26

Gene sequence:

>1086_bases
ATGCCAGCGCTTGAGCAGCCGCCCAATATCATGATAGTGGACGACACTCCTGCCAATCTCCGGCTTCTTGAAACGATGCT
GCAGGAAAAGAATTACCGCGTGCTGTTGTTTCCACGGGCGGATATCGCCCTGAAAGCGGCGAAGAAAAATCCCCCCGACC
TGGTGCTGCTCGACATCACCATGCCGGGAATGGATGGCTTCCAGTTTTGCGCGAGCCTGAAGGCGGACAGCCGTTTATGC
TCCACTCCGGTAATCTTTCTCAGCGGCCTGAACGAGCCGGCGGATAAGGTCAAGGCGTTCAGCCTGGGGGGGGTGGATTA
CATAACCAAGCCGTTCCAGCTCGAAGAGGTCCATGCGCGGGTGGAAACCCATCTCAACCTTCGTCGTCTGCAGATGGAAG
TGGAATCCCATAACCGCAACCTTGAGAGCCAAGTTGAGGCGTATGCGAAAGAGATTATCGCCTCCCAGATGGCGACCATT
TTCGCCCTGGCGAAACTTGCCGAGTCCCGCGATGACAACACCGGCAAGCACGTCGAACGGGTTCAGATTTACTGCCGCCT
GCTTGCGCAGCGACTGGCGGAAAAGGGACCGTACCGGGGAGAAGTCACCCCGGAATTCATCAACAACATAACCTTTGCAA
GCCCGCTCCATGACATAGGAAAGGTGGCCATAGTCGACCGCGTGCTGCTTAAGCCCGACAAACTGACAGCGGAAGAATTC
AAGATCATGCAGACCCATACGGTAATCGGAGCGCAAACCCTTGCCACGGCCCTGGCAAAATATCCGCAGAACGAGTTTCT
CCGTACCGGCATTGCCATAGCGCAGTCACATCACGAATGGTGGGATGGCACCGGTTACCCCGACGGGATCAAGGGGAGGG
AAATCCCGCTCCATGCCCGCATCACCGCCGTCGCGGACGTTTACGACGCACTCCGTTCCAATCGATGCTATCGGTTGTCA
CTACCCCATGAGGAAGTCCGTGCAATGATTCTGGAAGGATCAGGCACCCAGTTCGACCCGGATGTGGTTGCAGCGTTCAT
CGAATCCGAGGATGAATTCTTTGCGATAAGCTGCGAATTGGGCTGA

Upstream 100 bases:

>100_bases
GGCGAGTCCGAACCGATACCGGCCGAAGCGTTACGCCGCCTTGTCGATTCTTATTCCTACGACGAACTGACCACACTGCT
TGCAGGGGGGGACGCCAGAT

Downstream 100 bases:

>100_bases
TGGGGCACCGGTAGAGGCTGGTCGGCAACGGGAGACCTGGCGCTGAGGCAGTCGCAGGGCCGTCAGGCGTTTGCGTCGGT
CTTCTCGGCACTCGCATGCA

Product: response regulator receiver (CheY-like) modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MPALEQPPNIMIVDDTPANLRLLETMLQEKNYRVLLFPRADIALKAAKKNPPDLVLLDITMPGMDGFQFCASLKADSRLC
STPVIFLSGLNEPADKVKAFSLGGVDYITKPFQLEEVHARVETHLNLRRLQMEVESHNRNLESQVEAYAKEIIASQMATI
FALAKLAESRDDNTGKHVERVQIYCRLLAQRLAEKGPYRGEVTPEFINNITFASPLHDIGKVAIVDRVLLKPDKLTAEEF
KIMQTHTVIGAQTLATALAKYPQNEFLRTGIAIAQSHHEWWDGTGYPDGIKGREIPLHARITAVADVYDALRSNRCYRLS
LPHEEVRAMILEGSGTQFDPDVVAAFIESEDEFFAISCELG

Sequences:

>Translated_361_residues
MPALEQPPNIMIVDDTPANLRLLETMLQEKNYRVLLFPRADIALKAAKKNPPDLVLLDITMPGMDGFQFCASLKADSRLC
STPVIFLSGLNEPADKVKAFSLGGVDYITKPFQLEEVHARVETHLNLRRLQMEVESHNRNLESQVEAYAKEIIASQMATI
FALAKLAESRDDNTGKHVERVQIYCRLLAQRLAEKGPYRGEVTPEFINNITFASPLHDIGKVAIVDRVLLKPDKLTAEEF
KIMQTHTVIGAQTLATALAKYPQNEFLRTGIAIAQSHHEWWDGTGYPDGIKGREIPLHARITAVADVYDALRSNRCYRLS
LPHEEVRAMILEGSGTQFDPDVVAAFIESEDEFFAISCELG
>Mature_360_residues
PALEQPPNIMIVDDTPANLRLLETMLQEKNYRVLLFPRADIALKAAKKNPPDLVLLDITMPGMDGFQFCASLKADSRLCS
TPVIFLSGLNEPADKVKAFSLGGVDYITKPFQLEEVHARVETHLNLRRLQMEVESHNRNLESQVEAYAKEIIASQMATIF
ALAKLAESRDDNTGKHVERVQIYCRLLAQRLAEKGPYRGEVTPEFINNITFASPLHDIGKVAIVDRVLLKPDKLTAEEFK
IMQTHTVIGAQTLATALAKYPQNEFLRTGIAIAQSHHEWWDGTGYPDGIKGREIPLHARITAVADVYDALRSNRCYRLSL
PHEEVRAMILEGSGTQFDPDVVAAFIESEDEFFAISCELG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=143, Percent_Identity=34.2657342657343, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786911, Length=118, Percent_Identity=35.5932203389831, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=34.7107438016529, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789149, Length=109, Percent_Identity=36.697247706422, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=32.4561403508772, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788550, Length=134, Percent_Identity=31.3432835820896, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=31.8965517241379, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI145693140, Length=102, Percent_Identity=34.3137254901961, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790552, Length=126, Percent_Identity=31.7460317460317, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=30.7692307692308, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787229, Length=119, Percent_Identity=27.7310924369748, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 40384; Mature: 40253

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALEQPPNIMIVDDTPANLRLLETMLQEKNYRVLLFPRADIALKAAKKNPPDLVLLDIT
CCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCEEEEEEE
MPGMDGFQFCASLKADSRLCSTPVIFLSGLNEPADKVKAFSLGGVDYITKPFQLEEVHAR
CCCCCHHHHHHHHCCCCHHHCCCEEEEECCCCCHHHHEEEECCCHHHHCCCCHHHHHHHH
VETHLNLRRLQMEVESHNRNLESQVEAYAKEIIASQMATIFALAKLAESRDDNTGKHVER
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
VQIYCRLLAQRLAEKGPYRGEVTPEFINNITFASPLHDIGKVAIVDRVLLKPDKLTAEEF
HHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
KIMQTHTVIGAQTLATALAKYPQNEFLRTGIAIAQSHHEWWDGTGYPDGIKGREIPLHAR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCHHH
ITAVADVYDALRSNRCYRLSLPHEEVRAMILEGSGTQFDPDVVAAFIESEDEFFAISCEL
HHHHHHHHHHHHCCCEEEEECCHHHHHEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEEC
G
C
>Mature Secondary Structure 
PALEQPPNIMIVDDTPANLRLLETMLQEKNYRVLLFPRADIALKAAKKNPPDLVLLDIT
CCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCEEEEEEE
MPGMDGFQFCASLKADSRLCSTPVIFLSGLNEPADKVKAFSLGGVDYITKPFQLEEVHAR
CCCCCHHHHHHHHCCCCHHHCCCEEEEECCCCCHHHHEEEECCCHHHHCCCCHHHHHHHH
VETHLNLRRLQMEVESHNRNLESQVEAYAKEIIASQMATIFALAKLAESRDDNTGKHVER
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
VQIYCRLLAQRLAEKGPYRGEVTPEFINNITFASPLHDIGKVAIVDRVLLKPDKLTAEEF
HHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
KIMQTHTVIGAQTLATALAKYPQNEFLRTGIAIAQSHHEWWDGTGYPDGIKGREIPLHAR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCHHH
ITAVADVYDALRSNRCYRLSLPHEEVRAMILEGSGTQFDPDVVAAFIESEDEFFAISCEL
HHHHHHHHHHHHCCCEEEEECCHHHHHEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEEC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]