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The map label for this gene is int [H]

Identifier: 78224199

GI number: 78224199

Start: 3390705

End: 3391709

Strand: Direct

Name: int [H]

Synonym: Gmet_3006

Alternate gene names: 78224199

Gene position: 3390705-3391709 (Clockwise)

Preceding gene: 78224192

Following gene: 78224200

Centisome position: 84.82

GC content: 62.59

Gene sequence:

>1005_bases
ATGCACCCCATCAACATGGGCAAAAACGCCTCTGCTGTCGAACCCGAAAAACCCCGCCTCCTGGATATCCTCCGCACCAG
AATCCGCCTCAAACATTACAGCCTCCGCACGGAGCAGACTTACACCGAATGGGCGAAGCGCTACATTCTCTTCCATGGCA
AACGCCATCCCGCCGAGATGGGGAAGGAAGAGGTGGAGGCGTTTCTCTCCCATCTGGCGGTGAAGCACAATGTCTCGGCC
TCGACCCAGAGCCAAGCCCGGGCCGCGATTCTTTTTCTCTACCGCGAGGTACTCGACATTGAACTCCCCTGGCTGGACGA
TGTGGAACAGGCGAAGAAGCCCCAACGGCTGCCGGTGGTGCTGACGGTAAGCGAGGTGCAGGCTCTCCTCTCCCGCCTGA
CCGGAACCCATGCGCTGATGGGGCGACTGATGTACGGTACCGGCATGCGCCTGATGGAGACGGTGCGGCTGCGGGTGAAG
GATGTGGATTTCGAGCGGAGCGAGATCATCGTCCGGGAAGGTAAGGGTGCAAAGGATCGGATCACCATGCTCCCCCAAGC
GGTTGCGGCCCCGCTGCGGGAGCACCTGGGGCGGGTGGAGGCTCTTCACGGGGCGGATTTGAGGGCCGGCTTCGGCGAGG
TCCACCTCCCCCATGCCCTCGGGAAGAAGTACCCGAACGCGGGTCGGGAATGGGGATGGCAGTATGTGTTCCCGGCAAAG
CAGCTTTCGACGGACCCGCGCACGGGGGTAACCCGTCGGCACCACGCTGACGAGAAGGGAATCCAGCGGGCCATCCGTCA
GGCAGTGCGGGACGCGGAGCTTGCCAAGCCAGCCACTCCCCACACGCTACGGCACTCCTTCGCGACGCACCTGCTCCAGT
CGGGCTACGATATCCGCACGGTGCAGGAACTTCTGGGCCACAAGGATGTGCAGACGACGATGATCTACACCCATGTGCTG
AACCGTGGCGGCAAAGGGGTGGAAAGTCCCCTTGATCGGCTGTAG

Upstream 100 bases:

>100_bases
TTGGACGCCCTAAAAGGCGTGGAATTTTGCGCCAGTATGGTGTATAAGGCAGTCTTGAGTCAAGCTAATTAATGAATTCT
ACTCGTAAAGGGATTCTGAA

Downstream 100 bases:

>100_bases
AATTGTCCGGTAACGTCATTGCCACGGAGGTAACGCCATGAGGGAAAAGATCGAGATCATACCGGGGGATATCACGAAGC
TGGCGGTGGACACCATTGTG

Product: integron integrase

Products: NA

Alternate protein names: E2 protein [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MHPINMGKNASAVEPEKPRLLDILRTRIRLKHYSLRTEQTYTEWAKRYILFHGKRHPAEMGKEEVEAFLSHLAVKHNVSA
STQSQARAAILFLYREVLDIELPWLDDVEQAKKPQRLPVVLTVSEVQALLSRLTGTHALMGRLMYGTGMRLMETVRLRVK
DVDFERSEIIVREGKGAKDRITMLPQAVAAPLREHLGRVEALHGADLRAGFGEVHLPHALGKKYPNAGREWGWQYVFPAK
QLSTDPRTGVTRRHHADEKGIQRAIRQAVRDAELAKPATPHTLRHSFATHLLQSGYDIRTVQELLGHKDVQTTMIYTHVL
NRGGKGVESPLDRL

Sequences:

>Translated_334_residues
MHPINMGKNASAVEPEKPRLLDILRTRIRLKHYSLRTEQTYTEWAKRYILFHGKRHPAEMGKEEVEAFLSHLAVKHNVSA
STQSQARAAILFLYREVLDIELPWLDDVEQAKKPQRLPVVLTVSEVQALLSRLTGTHALMGRLMYGTGMRLMETVRLRVK
DVDFERSEIIVREGKGAKDRITMLPQAVAAPLREHLGRVEALHGADLRAGFGEVHLPHALGKKYPNAGREWGWQYVFPAK
QLSTDPRTGVTRRHHADEKGIQRAIRQAVRDAELAKPATPHTLRHSFATHLLQSGYDIRTVQELLGHKDVQTTMIYTHVL
NRGGKGVESPLDRL
>Mature_334_residues
MHPINMGKNASAVEPEKPRLLDILRTRIRLKHYSLRTEQTYTEWAKRYILFHGKRHPAEMGKEEVEAFLSHLAVKHNVSA
STQSQARAAILFLYREVLDIELPWLDDVEQAKKPQRLPVVLTVSEVQALLSRLTGTHALMGRLMYGTGMRLMETVRLRVK
DVDFERSEIIVREGKGAKDRITMLPQAVAAPLREHLGRVEALHGADLRAGFGEVHLPHALGKKYPNAGREWGWQYVFPAK
QLSTDPRTGVTRRHHADEKGIQRAIRQAVRDAELAKPATPHTLRHSFATHLLQSGYDIRTVQELLGHKDVQTTMIYTHVL
NRGGKGVESPLDRL

Specific function: Putative integrase believed to be involved in the insertion of antibiotic resistance genes into plasmids and transposons [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1789261, Length=253, Percent_Identity=31.6205533596838, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790244, Length=217, Percent_Identity=35.0230414746544, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR011946
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 37936; Mature: 37936

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPINMGKNASAVEPEKPRLLDILRTRIRLKHYSLRTEQTYTEWAKRYILFHGKRHPAEM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHC
GKEEVEAFLSHLAVKHNVSASTQSQARAAILFLYREVLDIELPWLDDVEQAKKPQRLPVV
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEE
LTVSEVQALLSRLTGTHALMGRLMYGTGMRLMETVRLRVKDVDFERSEIIVREGKGAKDR
EEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHEEEECCCCCHHH
ITMLPQAVAAPLREHLGRVEALHGADLRAGFGEVHLPHALGKKYPNAGREWGWQYVFPAK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHCCCCCCCHHCCCEEECCHH
QLSTDPRTGVTRRHHADEKGIQRAIRQAVRDAELAKPATPHTLRHSFATHLLQSGYDIRT
HHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHH
VQELLGHKDVQTTMIYTHVLNRGGKGVESPLDRL
HHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHCC
>Mature Secondary Structure
MHPINMGKNASAVEPEKPRLLDILRTRIRLKHYSLRTEQTYTEWAKRYILFHGKRHPAEM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHC
GKEEVEAFLSHLAVKHNVSASTQSQARAAILFLYREVLDIELPWLDDVEQAKKPQRLPVV
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEE
LTVSEVQALLSRLTGTHALMGRLMYGTGMRLMETVRLRVKDVDFERSEIIVREGKGAKDR
EEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHEEEECCCCCHHH
ITMLPQAVAAPLREHLGRVEALHGADLRAGFGEVHLPHALGKKYPNAGREWGWQYVFPAK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHCCCCCCCHHCCCEEECCHH
QLSTDPRTGVTRRHHADEKGIQRAIRQAVRDAELAKPATPHTLRHSFATHLLQSGYDIRT
HHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHH
VQELLGHKDVQTTMIYTHVLNRGGKGVESPLDRL
HHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2821509; 2823258; 3054482; 8195081; 2188588 [H]