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The map label for this gene is glyS

Identifier: 78224137

GI number: 78224137

Start: 3330385

End: 3332445

Strand: Reverse

Name: glyS

Synonym: Gmet_2941

Alternate gene names: 78224137

Gene position: 3332445-3330385 (Counterclockwise)

Preceding gene: 78224138

Following gene: 78224136

Centisome position: 83.36

GC content: 65.65

Gene sequence:

>2061_bases
ATGAAAGAGTTGTTCCTTGAGATAGGCACCGAAGAGATCCCGGCGGGCTTCATCCCGAAGGCCATGGCCGATATGGAGGC
CCTGCTGGCGAAGGAGCTTGAGAATGCCCGCATCTCCTTCGACGACATCCGGACCCTCGGCACCCCCCGTCGCCTGGCCC
TGACGGTGAAGGGTCTTCCGACGGTCCAGCCCGATGCGGAGATCACTGCCATGGGGCCGGCCAGGAACGTAGCCTTCGAC
GCCGAAGGAAAACCCACCAGGGCTGCCGAAGGGTTTGCCCGGGGGCAGGGGGTGGACGTGGCGTCCCTCACCCTCGTCGC
CACTGAAAAGGGTGAGTACGTGGCCGCCGTCCGCAAGGAGAGCGGTCGCCCCGTGCCGGAGCTCCTGGCCGAGATCCTGC
CGCGGCTTGTTGCGAATATCCCTTTCCGCAAGTCCATGCGCTGGGGTGCTCTCGACGTCCGCTTCGCCCGCCCCATCCAC
TGGATCGTGGCCCTCTTTGACGGCGTCGTGGTTCCCTTCACCTTCGGGAACATCGAAAGCGGCACCATTTCCCGGGGGCA
CCGCTTCATGGCCAACCAGCCCTTCCCGGTGCGGGACTTTGCCCACTATCTGGAGGAGTGCGAGCGCCACTTCGTCATTC
CCGACCCGGAACGGAGACAGGAGATCATCCGGCGGGAGATCCACCGGGTTGCCAAGGCCGCCGGCGGGCATCTTCTCCCC
GACGAGGGGCTTCTGGAGGAGGTCTCCTTCCTCTGCGAGTACCCGAGCGCGGTTCACGGCACCTTTTCCGCCGAGTTCCT
GAAGGTTCCCCGGGAGGTTCTCATCACCTCCATGCGGAGCCACCAGCGTTACTTCTCCATTGTCGACGACGCCGGTAAGC
TCATGCCCGGTTTCATCACCATCAACAACACCCTCACCGAGGATCCCACCGTGGTGGTCAAGGGGAACGAGCGGGTGCTC
AGGGCCCGGCTCTCCGATGCCCGTTTCTTCTTCGAGGAGGACCAGAAGGTCAAGCTGGAGACCCGGGTGGAGTCCCTGAA
GAACGTGGTCTACCAGCAAAAGCTCGGCACCTCCTTTGAAAAGATGGAGCGGTTCCGTGCCTTGGCCGAGGGACTTGCCG
ACCTCCTGAACCCGGCGGTGAAAGTGAAGGTTTCCCAGGCCGCGTTCCTCTGCAAGGCGGATCTCGTCTCCGGCATGGTG
GGTGAGTTCCCCGAGGTGCAGGGGATCATGGGGCGCGAGTACGCCCTCATCGAGGGGGAGGACGCCGAGGTGGCCGCGGC
CATCGCCGAGCACTACCTCCCGACCCAGGCGGGGGGCGAGCTGCCGGCGTCGGACATTGGCGCCTTCGTCTCCATGGCCG
ACAAGCTGGACACCATCTGCGGCTGCTTCGGCGTGGGGCTCATCCCCACCGGTTCCGCCGACCCCTACGCCCTGCGCCGC
TCGGCCCTGGGGATCATCAATATCATTCTCGACAAGGGGTACCGCCTCTCCCTGGAGGAGCAGGTGGACAAGGCCCTTGG
CCTTCTGGCCGCCAAGCTCACCCGGCCGGCCGTTGACGTGAAGGCCGACGTTCTGGAGTTCTTCCGGGGCCGGTTCGTGA
ACCTGATGGCCGACCGCCATGCCTCCGACGCGGTGGATGCCGCCGTGGCCGCCGGCTGCGCCGATCTCGTGGATGCCGCC
GCCCGGATCGCGGCCCTTTCGGAGTTCAGGTCGCACCCCGACTTCGAGCCTCTGGCCGTGGCCTTCAAGCGGGTCGGCAA
CATCGTGAAGGAAGGGGTGGACGCCCCTGTGGATACGGCTCTCTTCCAGGACGCCGCCGAGGGGCTCCTCAACGATGCCG
TCCAGGGGGTTGCCGTATCGGTGCGCGAAAAGATCGCAACCGGAGCCTATCTGGAAGCCCTCACGGAGATCGCCGCCCTG
CGGGGACCCGTCGACACCTTCTTCGACAAGGTCATGGTCATGGCCGAGGACGAACGGGTCCGGACCAACCGTCTGGCGCT
CCTGACCGGGATCGCCCGGATGCTCGGCGCCATCGCCGACTTCGCCAAGATAGCCGCCTAG

Upstream 100 bases:

>100_bases
TACATCGGCCGGGTGCGAAACGTGGCGCGCCTCTGTGCCGAAGGGTATCTGAAACTGCGCGAAAGCCTCGGCTTCCCGCT
CCTGAAAGGAGGCCGCTAGC

Downstream 100 bases:

>100_bases
CCCACGCTAGGCGGCTTTTTTTATGTTTTAGTCCGGCTGAATCTCTGTTGAGTTACGCTTAATCACATTTAATGCAGCTG
AAGGGTTTTGACTTTCAGTA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MKELFLEIGTEEIPAGFIPKAMADMEALLAKELENARISFDDIRTLGTPRRLALTVKGLPTVQPDAEITAMGPARNVAFD
AEGKPTRAAEGFARGQGVDVASLTLVATEKGEYVAAVRKESGRPVPELLAEILPRLVANIPFRKSMRWGALDVRFARPIH
WIVALFDGVVVPFTFGNIESGTISRGHRFMANQPFPVRDFAHYLEECERHFVIPDPERRQEIIRREIHRVAKAAGGHLLP
DEGLLEEVSFLCEYPSAVHGTFSAEFLKVPREVLITSMRSHQRYFSIVDDAGKLMPGFITINNTLTEDPTVVVKGNERVL
RARLSDARFFFEEDQKVKLETRVESLKNVVYQQKLGTSFEKMERFRALAEGLADLLNPAVKVKVSQAAFLCKADLVSGMV
GEFPEVQGIMGREYALIEGEDAEVAAAIAEHYLPTQAGGELPASDIGAFVSMADKLDTICGCFGVGLIPTGSADPYALRR
SALGIINIILDKGYRLSLEEQVDKALGLLAAKLTRPAVDVKADVLEFFRGRFVNLMADRHASDAVDAAVAAGCADLVDAA
ARIAALSEFRSHPDFEPLAVAFKRVGNIVKEGVDAPVDTALFQDAAEGLLNDAVQGVAVSVREKIATGAYLEALTEIAAL
RGPVDTFFDKVMVMAEDERVRTNRLALLTGIARMLGAIADFAKIAA

Sequences:

>Translated_686_residues
MKELFLEIGTEEIPAGFIPKAMADMEALLAKELENARISFDDIRTLGTPRRLALTVKGLPTVQPDAEITAMGPARNVAFD
AEGKPTRAAEGFARGQGVDVASLTLVATEKGEYVAAVRKESGRPVPELLAEILPRLVANIPFRKSMRWGALDVRFARPIH
WIVALFDGVVVPFTFGNIESGTISRGHRFMANQPFPVRDFAHYLEECERHFVIPDPERRQEIIRREIHRVAKAAGGHLLP
DEGLLEEVSFLCEYPSAVHGTFSAEFLKVPREVLITSMRSHQRYFSIVDDAGKLMPGFITINNTLTEDPTVVVKGNERVL
RARLSDARFFFEEDQKVKLETRVESLKNVVYQQKLGTSFEKMERFRALAEGLADLLNPAVKVKVSQAAFLCKADLVSGMV
GEFPEVQGIMGREYALIEGEDAEVAAAIAEHYLPTQAGGELPASDIGAFVSMADKLDTICGCFGVGLIPTGSADPYALRR
SALGIINIILDKGYRLSLEEQVDKALGLLAAKLTRPAVDVKADVLEFFRGRFVNLMADRHASDAVDAAVAAGCADLVDAA
ARIAALSEFRSHPDFEPLAVAFKRVGNIVKEGVDAPVDTALFQDAAEGLLNDAVQGVAVSVREKIATGAYLEALTEIAAL
RGPVDTFFDKVMVMAEDERVRTNRLALLTGIARMLGAIADFAKIAA
>Mature_686_residues
MKELFLEIGTEEIPAGFIPKAMADMEALLAKELENARISFDDIRTLGTPRRLALTVKGLPTVQPDAEITAMGPARNVAFD
AEGKPTRAAEGFARGQGVDVASLTLVATEKGEYVAAVRKESGRPVPELLAEILPRLVANIPFRKSMRWGALDVRFARPIH
WIVALFDGVVVPFTFGNIESGTISRGHRFMANQPFPVRDFAHYLEECERHFVIPDPERRQEIIRREIHRVAKAAGGHLLP
DEGLLEEVSFLCEYPSAVHGTFSAEFLKVPREVLITSMRSHQRYFSIVDDAGKLMPGFITINNTLTEDPTVVVKGNERVL
RARLSDARFFFEEDQKVKLETRVESLKNVVYQQKLGTSFEKMERFRALAEGLADLLNPAVKVKVSQAAFLCKADLVSGMV
GEFPEVQGIMGREYALIEGEDAEVAAAIAEHYLPTQAGGELPASDIGAFVSMADKLDTICGCFGVGLIPTGSADPYALRR
SALGIINIILDKGYRLSLEEQVDKALGLLAAKLTRPAVDVKADVLEFFRGRFVNLMADRHASDAVDAAVAAGCADLVDAA
ARIAALSEFRSHPDFEPLAVAFKRVGNIVKEGVDAPVDTALFQDAAEGLLNDAVQGVAVSVREKIATGAYLEALTEIAAL
RGPVDTFFDKVMVMAEDERVRTNRLALLTGIARMLGAIADFAKIAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=690, Percent_Identity=39.2753623188406, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_GEOMG (Q39RG5)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385884.1
- ProteinModelPortal:   Q39RG5
- STRING:   Q39RG5
- GeneID:   3740835
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2941
- NMPDR:   fig|269799.3.peg.3228
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   Q39RG5
- ProtClustDB:   PRK01233
- BioCyc:   GMET269799:GMET_2941-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 74760; Mature: 74760

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELFLEIGTEEIPAGFIPKAMADMEALLAKELENARISFDDIRTLGTPRRLALTVKGLP
CCCHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEECCCC
TVQPDAEITAMGPARNVAFDAEGKPTRAAEGFARGQGVDVASLTLVATEKGEYVAAVRKE
CCCCCCCEEECCCCCCCEECCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCEEEHEECC
SGRPVPELLAEILPRLVANIPFRKSMRWGALDVRFARPIHWIVALFDGVVVPFTFGNIES
CCCCHHHHHHHHHHHHHHCCCCHHCCCCCCEEEHHHHHHHHHHHHHCCEEEEEEECCCCC
GTISRGHRFMANQPFPVRDFAHYLEECERHFVIPDPERRQEIIRREIHRVAKAAGGHLLP
CCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DEGLLEEVSFLCEYPSAVHGTFSAEFLKVPREVLITSMRSHQRYFSIVDDAGKLMPGFIT
CCCHHHHHHHHHCCCHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
INNTLTEDPTVVVKGNERVLRARLSDARFFFEEDQKVKLETRVESLKNVVYQQKLGTSFE
ECCCCCCCCEEEEECCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCHH
KMERFRALAEGLADLLNPAVKVKVSQAAFLCKADLVSGMVGEFPEVQGIMGREYALIEGE
HHHHHHHHHHHHHHHCCCHHEEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEECC
DAEVAAAIAEHYLPTQAGGELPASDIGAFVSMADKLDTICGCFGVGLIPTGSADPYALRR
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
SALGIINIILDKGYRLSLEEQVDKALGLLAAKLTRPAVDVKADVLEFFRGRFVNLMADRH
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
ASDAVDAAVAAGCADLVDAAARIAALSEFRSHPDFEPLAVAFKRVGNIVKEGVDAPVDTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
LFQDAAEGLLNDAVQGVAVSVREKIATGAYLEALTEIAALRGPVDTFFDKVMVMAEDERV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
RTNRLALLTGIARMLGAIADFAKIAA
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKELFLEIGTEEIPAGFIPKAMADMEALLAKELENARISFDDIRTLGTPRRLALTVKGLP
CCCHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEECCCC
TVQPDAEITAMGPARNVAFDAEGKPTRAAEGFARGQGVDVASLTLVATEKGEYVAAVRKE
CCCCCCCEEECCCCCCCEECCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCEEEHEECC
SGRPVPELLAEILPRLVANIPFRKSMRWGALDVRFARPIHWIVALFDGVVVPFTFGNIES
CCCCHHHHHHHHHHHHHHCCCCHHCCCCCCEEEHHHHHHHHHHHHHCCEEEEEEECCCCC
GTISRGHRFMANQPFPVRDFAHYLEECERHFVIPDPERRQEIIRREIHRVAKAAGGHLLP
CCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DEGLLEEVSFLCEYPSAVHGTFSAEFLKVPREVLITSMRSHQRYFSIVDDAGKLMPGFIT
CCCHHHHHHHHHCCCHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
INNTLTEDPTVVVKGNERVLRARLSDARFFFEEDQKVKLETRVESLKNVVYQQKLGTSFE
ECCCCCCCCEEEEECCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCHH
KMERFRALAEGLADLLNPAVKVKVSQAAFLCKADLVSGMVGEFPEVQGIMGREYALIEGE
HHHHHHHHHHHHHHHCCCHHEEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEECC
DAEVAAAIAEHYLPTQAGGELPASDIGAFVSMADKLDTICGCFGVGLIPTGSADPYALRR
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
SALGIINIILDKGYRLSLEEQVDKALGLLAAKLTRPAVDVKADVLEFFRGRFVNLMADRH
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
ASDAVDAAVAAGCADLVDAAARIAALSEFRSHPDFEPLAVAFKRVGNIVKEGVDAPVDTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
LFQDAAEGLLNDAVQGVAVSVREKIATGAYLEALTEIAALRGPVDTFFDKVMVMAEDERV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
RTNRLALLTGIARMLGAIADFAKIAA
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA