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The map label for this gene is sucB [H]

Identifier: 78223962

GI number: 78223962

Start: 3125564

End: 3126820

Strand: Reverse

Name: sucB [H]

Synonym: Gmet_2766

Alternate gene names: 78223962

Gene position: 3126820-3125564 (Counterclockwise)

Preceding gene: 78223963

Following gene: 78223961

Centisome position: 78.22

GC content: 64.28

Gene sequence:

>1257_bases
ATGGAAATAAAGGTTCCGTCAGTGGGCGAATCGGTCTACGAGGCACTGGTGGGGAAGTGGCTGAAAAAGAACGGCGAGGC
GGTCCGGAAGGACGAGCCGGTCTGCGAGATCGAGACCGACAAGATCACCATGGAGATCGACGCCGGGGCCGACGGTGTGC
TCACCATCATGGTGCCGGAAGGGGCCACGGTGAAGATCGGCTCCGTCATCGGCATTATAGAAGCCGGGACCGGGGACCGG
GGACCGGGGACCGGGAAAGGCAAGGAGGTGCCTCCACTGTCGCCGGCGGTTCGCAAGATTGCCCAGGAGCTGGGGATTAA
GCCGGAAACTGTCCATGGAAGCGGCCGGGGCGGGCGGGTGACGGTGGATGATCTCTTAACGGCCGGGACCAGGGACTTGA
CAGCCGGGACGGGGGACCGGGGACCGGGGACCGGGAAAGGCGGGGAAGTGCCGAAGGATGCTGCGGTGAAATCGGGTGAA
GCGGCGGCGGTTGGGGGTAAGCAGAAAGAATTTGCTTTTGAGCCGGTCCCCGGTCCCCGGTCCCCGGTCCCCGCGGCAGA
GCGCGTTACGCGCTCGCCGATGACCCCCATCCGCAAACGGATCGCCGAGCGGCTCCTGGTGGCCCGGCAGCAGACCGCCA
TGCTCACCACGTTCAACGAGGCGGATCTGGGCCGGGTGATGGAACTGCGGAAGAAGTACAAGGAGCATTTCCAGAAGAAG
CACGGCGTCTCCCTCGGTTTCATGTCGTTCTTCGTGAAGGCGTGCGTGGAGGCCCTGAAGGAGTATCCGGCGGTGAACGG
CTCCATCGAGGGGGATGACATCGTCTTCCACCACTACTACCATATCGGCATCGCCATTGGGGCCGAGAAGGGGCTCGTGG
TGCCGGTGCTCCGGGACGCCGACCGGCTCTCCTTTGCCGAGATCGAGACGACCATCGCCGGGTTCGCGGAAAAGACCAAG
GCCAACCGGCTGGAGCTGTCGGATCTCCAGGGGGGGACTTTCACCATCTCCAACGGCGGGGTCTACGGCTCGCTCCTCTC
CACTCCCATTCTCAACCCGCCCCAGAGCGGCGTCCTCGGCATGCACGCCGTCCAGGAGCGCCCCGTGGTCCGCGACGGCC
AGATCGTCATACGTCCCATGATGTACCTGGCCCTCTCCTACGACCACCGGATCATCGACGGCCGCGAGGCGGTCGGGTTC
CTGAAGAAGGTGAAGGAGTATGTGGAAGAGCCCGAGGAACTCTTCCTTGAAGGGTAG

Upstream 100 bases:

>100_bases
CGGGAGTACAACGACGGTATCTGGGGGCTCCAGCCTGTGCCGGTACTTGCTGCAAGGTAACCGGACCTGCTTTTTGTAAT
GTGAGAGAGAGGTGTCCCCT

Downstream 100 bases:

>100_bases
ATTGACCGCCGCAGGGACCTGCTCACTGCTGGCGGCCCTTCTCCTGGCCGCTCCTGATCCGTCGGCATCGGCCCGGGTCG
GCAGGAATGGCAAAACACCT

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MEIKVPSVGESVYEALVGKWLKKNGEAVRKDEPVCEIETDKITMEIDAGADGVLTIMVPEGATVKIGSVIGIIEAGTGDR
GPGTGKGKEVPPLSPAVRKIAQELGIKPETVHGSGRGGRVTVDDLLTAGTRDLTAGTGDRGPGTGKGGEVPKDAAVKSGE
AAAVGGKQKEFAFEPVPGPRSPVPAAERVTRSPMTPIRKRIAERLLVARQQTAMLTTFNEADLGRVMELRKKYKEHFQKK
HGVSLGFMSFFVKACVEALKEYPAVNGSIEGDDIVFHHYYHIGIAIGAEKGLVVPVLRDADRLSFAEIETTIAGFAEKTK
ANRLELSDLQGGTFTISNGGVYGSLLSTPILNPPQSGVLGMHAVQERPVVRDGQIVIRPMMYLALSYDHRIIDGREAVGF
LKKVKEYVEEPEELFLEG

Sequences:

>Translated_418_residues
MEIKVPSVGESVYEALVGKWLKKNGEAVRKDEPVCEIETDKITMEIDAGADGVLTIMVPEGATVKIGSVIGIIEAGTGDR
GPGTGKGKEVPPLSPAVRKIAQELGIKPETVHGSGRGGRVTVDDLLTAGTRDLTAGTGDRGPGTGKGGEVPKDAAVKSGE
AAAVGGKQKEFAFEPVPGPRSPVPAAERVTRSPMTPIRKRIAERLLVARQQTAMLTTFNEADLGRVMELRKKYKEHFQKK
HGVSLGFMSFFVKACVEALKEYPAVNGSIEGDDIVFHHYYHIGIAIGAEKGLVVPVLRDADRLSFAEIETTIAGFAEKTK
ANRLELSDLQGGTFTISNGGVYGSLLSTPILNPPQSGVLGMHAVQERPVVRDGQIVIRPMMYLALSYDHRIIDGREAVGF
LKKVKEYVEEPEELFLEG
>Mature_418_residues
MEIKVPSVGESVYEALVGKWLKKNGEAVRKDEPVCEIETDKITMEIDAGADGVLTIMVPEGATVKIGSVIGIIEAGTGDR
GPGTGKGKEVPPLSPAVRKIAQELGIKPETVHGSGRGGRVTVDDLLTAGTRDLTAGTGDRGPGTGKGGEVPKDAAVKSGE
AAAVGGKQKEFAFEPVPGPRSPVPAAERVTRSPMTPIRKRIAERLLVARQQTAMLTTFNEADLGRVMELRKKYKEHFQKK
HGVSLGFMSFFVKACVEALKEYPAVNGSIEGDDIVFHHYYHIGIAIGAEKGLVVPVLRDADRLSFAEIETTIAGFAEKTK
ANRLELSDLQGGTFTISNGGVYGSLLSTPILNPPQSGVLGMHAVQERPVVRDGQIVIRPMMYLALSYDHRIIDGREAVGF
LKKVKEYVEEPEELFLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=52.8384279475983, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI203098753, Length=458, Percent_Identity=27.7292576419214, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI203098816, Length=459, Percent_Identity=28.322440087146, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI110671329, Length=439, Percent_Identity=26.1958997722096, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=28.3446712018141, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=29.8136645962733, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1786946, Length=421, Percent_Identity=45.6057007125891, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1786305, Length=452, Percent_Identity=25.4424778761062, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25146366, Length=420, Percent_Identity=38.3333333333333, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17537937, Length=446, Percent_Identity=25.1121076233184, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=25.8426966292135, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=30.0884955752212, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=416, Percent_Identity=41.5865384615385, Blast_Score=295, Evalue=9e-81,
Organism=Saccharomyces cerevisiae, GI6324258, Length=231, Percent_Identity=26.8398268398268, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=52.4886877828054, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI18859875, Length=451, Percent_Identity=26.3858093126386, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24582497, Length=234, Percent_Identity=27.7777777777778, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI20129315, Length=234, Percent_Identity=27.7777777777778, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44848; Mature: 44848

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKVPSVGESVYEALVGKWLKKNGEAVRKDEPVCEIETDKITMEIDAGADGVLTIMVPE
CEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEECCCCCEEEEEECC
GATVKIGSVIGIIEAGTGDRGPGTGKGKEVPPLSPAVRKIAQELGIKPETVHGSGRGGRV
CCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCEE
TVDDLLTAGTRDLTAGTGDRGPGTGKGGEVPKDAAVKSGEAAAVGGKQKEFAFEPVPGPR
EHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCEECCCCCCC
SPVPAAERVTRSPMTPIRKRIAERLLVARQQTAMLTTFNEADLGRVMELRKKYKEHFQKK
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHH
HGVSLGFMSFFVKACVEALKEYPAVNGSIEGDDIVFHHYYHIGIAIGAEKGLVVPVLRDA
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCC
DRLSFAEIETTIAGFAEKTKANRLELSDLQGGTFTISNGGVYGSLLSTPILNPPQSGVLG
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEHHHHHCCCCCCCCCCCCH
MHAVQERPVVRDGQIVIRPMMYLALSYDHRIIDGREAVGFLKKVKEYVEEPEELFLEG
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MEIKVPSVGESVYEALVGKWLKKNGEAVRKDEPVCEIETDKITMEIDAGADGVLTIMVPE
CEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEECCCCCEEEEEECC
GATVKIGSVIGIIEAGTGDRGPGTGKGKEVPPLSPAVRKIAQELGIKPETVHGSGRGGRV
CCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCEE
TVDDLLTAGTRDLTAGTGDRGPGTGKGGEVPKDAAVKSGEAAAVGGKQKEFAFEPVPGPR
EHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCEECCCCCCC
SPVPAAERVTRSPMTPIRKRIAERLLVARQQTAMLTTFNEADLGRVMELRKKYKEHFQKK
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHH
HGVSLGFMSFFVKACVEALKEYPAVNGSIEGDDIVFHHYYHIGIAIGAEKGLVVPVLRDA
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCC
DRLSFAEIETTIAGFAEKTKANRLELSDLQGGTFTISNGGVYGSLLSTPILNPPQSGVLG
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEHHHHHCCCCCCCCCCCCH
MHAVQERPVVRDGQIVIRPMMYLALSYDHRIIDGREAVGFLKKVKEYVEEPEELFLEG
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]