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The map label for this gene is lipB

Identifier: 78223943

GI number: 78223943

Start: 3106581

End: 3107222

Strand: Reverse

Name: lipB

Synonym: Gmet_2747

Alternate gene names: 78223943

Gene position: 3107222-3106581 (Counterclockwise)

Preceding gene: 78223944

Following gene: 78223942

Centisome position: 77.73

GC content: 66.82

Gene sequence:

>642_bases
ATGATGATTCGTGACCTGGGGAAGTTGGAGTTCGAGGCGGCGCTGGCCATTCAGGAACAACTGGCGGCCGGGGTGGCTGC
CGGAACGTCGCCGGAGACGCTTCTCCTCCTGGAGCATCCGCCGGTCTACACTATTGGTCGGGGCGGTTGCCGGGAGAATG
TGCTCGACCCCGGAATCCGGGTGGTGGAAGCCAACCGTGGCGGCGACGTGACGTTCCACGGGCCGGGGCAACTGGTCGGC
TACCCCGTCATCGACCTGGGGCGCCGTGGCCGCGACCTTCACCGCTACCTCCGTATCCTCGAAGAACTACTCATCAATGT
GGCGGCCGAATTCGGCGTCGCCGCCCGCCGCGAACCAGGCCGCACCGGCATCTGGACCGACGGGGGGAAGCTGGCTTCCA
TCGGCGTCGGCGTTCGCCGCTGGGTGACGATGCACGGTTTCGCCCTCAACGTCACCAACGAATTGGCCCCCTTCCGCTCC
ATCAACCCCTGCGGCATTGTTGCCTGCCCCATCACCTCTCTCTCCGCCATCCTCGGTCGCCCCGTCCCCCTGGGTGACGT
CCGCCGTTCGGTGGCCGGGCGGTTTCCGTTCCTCCTCGACGAGCGGCTTCCTTTCCTTGAGACGGCTGAACCGGCCCCCT
GA

Upstream 100 bases:

>100_bases
GTACCATCCGGGCCGAGGGAAGGCCCGGGGAAGGCGCCTGCTTCTCATTCACCCTTGGCAGCGAAACACCCCCTGTCCCG
CCGAACGTCGCCGACCCGGC

Downstream 100 bases:

>100_bases
CCTTACAAATCCTGAAGGCGTGGTATACTTGTTCAATTCAAATGCAATGAATAAGGAGGCCCGTCATGCCACTCCCCAGC
CAGAGAAAACTTCTTCCCGA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MMIRDLGKLEFEAALAIQEQLAAGVAAGTSPETLLLLEHPPVYTIGRGGCRENVLDPGIRVVEANRGGDVTFHGPGQLVG
YPVIDLGRRGRDLHRYLRILEELLINVAAEFGVAARREPGRTGIWTDGGKLASIGVGVRRWVTMHGFALNVTNELAPFRS
INPCGIVACPITSLSAILGRPVPLGDVRRSVAGRFPFLLDERLPFLETAEPAP

Sequences:

>Translated_213_residues
MMIRDLGKLEFEAALAIQEQLAAGVAAGTSPETLLLLEHPPVYTIGRGGCRENVLDPGIRVVEANRGGDVTFHGPGQLVG
YPVIDLGRRGRDLHRYLRILEELLINVAAEFGVAARREPGRTGIWTDGGKLASIGVGVRRWVTMHGFALNVTNELAPFRS
INPCGIVACPITSLSAILGRPVPLGDVRRSVAGRFPFLLDERLPFLETAEPAP
>Mature_213_residues
MMIRDLGKLEFEAALAIQEQLAAGVAAGTSPETLLLLEHPPVYTIGRGGCRENVLDPGIRVVEANRGGDVTFHGPGQLVG
YPVIDLGRRGRDLHRYLRILEELLINVAAEFGVAARREPGRTGIWTDGGKLASIGVGVRRWVTMHGFALNVTNELAPFRS
INPCGIVACPITSLSAILGRPVPLGDVRRSVAGRFPFLLDERLPFLETAEPAP

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=200, Percent_Identity=39.5, Blast_Score=91, Evalue=7e-19,
Organism=Escherichia coli, GI87081767, Length=176, Percent_Identity=35.7954545454545, Blast_Score=118, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21355483, Length=207, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_GEOMG (Q39S09)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385690.1
- ProteinModelPortal:   Q39S09
- SMR:   Q39S09
- STRING:   Q39S09
- GeneID:   3740015
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2747
- NMPDR:   fig|269799.3.peg.2725
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HIEMSEF
- PhylomeDB:   Q39S09
- ProtClustDB:   CLSK828834
- BioCyc:   GMET269799:GMET_2747-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 22962; Mature: 22962

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIRDLGKLEFEAALAIQEQLAAGVAAGTSPETLLLLEHPPVYTIGRGGCRENVLDPGIR
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCE
VVEANRGGDVTFHGPGQLVGYPVIDLGRRGRDLHRYLRILEELLINVAAEFGVAARREPG
EEECCCCCEEEECCCCCEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCC
RTGIWTDGGKLASIGVGVRRWVTMHGFALNVTNELAPFRSINPCGIVACPITSLSAILGR
CCCEECCCCEEEEHHHHHHHHHHHCCEEEECCHHCCCCCCCCCCCEEEECHHHHHHHHCC
PVPLGDVRRSVAGRFPFLLDERLPFLETAEPAP
CCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MMIRDLGKLEFEAALAIQEQLAAGVAAGTSPETLLLLEHPPVYTIGRGGCRENVLDPGIR
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCE
VVEANRGGDVTFHGPGQLVGYPVIDLGRRGRDLHRYLRILEELLINVAAEFGVAARREPG
EEECCCCCEEEECCCCCEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCC
RTGIWTDGGKLASIGVGVRRWVTMHGFALNVTNELAPFRSINPCGIVACPITSLSAILGR
CCCEECCCCEEEEHHHHHHHHHHHCCEEEECCHHCCCCCCCCCCCEEEECHHHHHHHHCC
PVPLGDVRRSVAGRFPFLLDERLPFLETAEPAP
CCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA