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The map label for this gene is sir2 [H]

Identifier: 78223719

GI number: 78223719

Start: 2862590

End: 2863897

Strand: Reverse

Name: sir2 [H]

Synonym: Gmet_2520

Alternate gene names: 78223719

Gene position: 2863897-2862590 (Counterclockwise)

Preceding gene: 78223725

Following gene: 78223718

Centisome position: 71.64

GC content: 64.14

Gene sequence:

>1308_bases
ATGATGTTCGACCCGCAAAGCGTGCGGATAGACGGGGTGTACGAGCAACGGCAACCGGGCAATTTCATGCTCCGGATCAA
GGTTCCGGCAGGGATCATCTCCACGGAACAGGCCCTCAGGGTGGCTGAGATCGCCGACCGGTTCGCCGGCGGTTCGGTTC
ATCTGACTACCCGGGGAAGTATTGAGCTCCATTGGCTGAAACAGGAAAACCTGGCGGAAGCATGGCGGATGCTGGCATCG
GTGGGACTGACCACGCGGGGAGCATGCGGCGGCGCGGTGCGCGGCGTGGTCTGCGCCATGCCCGGCGATGCCGGCTATGG
CGCTGCCCAACTCCTGGCGCGCCGGCTTCTGGCGCATTTCACCCACAACCCCCGGTTCGAAGGGCTCCCCAAGAAGTTCA
AGATCGGCGTATTCACCGGTTACGGTGACGGGCGGCACCTGATCCAGGACCTTTCCGTCATTCTTCGGGAAGGGGCCGAT
GGGAACAGCAGCTATGACGTTTGGATCGCGGGAGGGCTCGGACGGGAGCCCCACGCATCCTTTCTCCTGGAAGAGGGAGT
TGCGGGAGAGCGCCTCATCCCCCTGGCCGAGGCGATCATTTCCCTGTACCGGGAATTGACCCCCAAGGGGAAGCGGCTCA
AGCATCTCGTGCGGGAACGAGGTCGTGAGGAGTTCGTGAAGCTCGTTCGGGAGCGTGTCGCGGCGATTCCCCCTCTCTCC
CTCGGCGCAGGGCTTGCCACCCCCCTGACGTCTGCCCCTCGCGAGGGAGCGGCCGAGCGGCTCACGGCGGGGATATTTGC
CGGCGAACTGGGGACGAAGAGGTTTGTTGACCTTGCGGAGATCGCACGGCAGTATGCAGGGGGCTACATGGTCGTCACAG
GGGAACAGAATGTCCTGTTTATCCTCGATGATGGGGCGCTACGCGATGATGCGGCCCGGGCCCTGGCGGCGGCCGGCTTT
GGCTGCGATTCTCCGGCGGAGAGGGTTTGCTTCCGGATCTGCCCCGGCAACCACGAGTGCAAGATGGGTCTCTCGCCAAC
CCGCGATGTGGCCCGGGAGCTTGCCGGTACTCTGGGGGAGGCGGGGGAACGCCTCTCCTGGTCCATTTGCGGCTGCCCCA
ACTCCTGCTCCCATCCCCAACTGGCCGACGTGGGGATAGCCACCGTGAAAAGTGTCAAGGACGAACAGGGCGAGCGGCAT
CCCCTCTTCGATCTCTATCGCCGCTACGGCACCGACGGCTTCGGCGTTGTAGTCCGTCATGGTATCGGCATTGACGAGCT
TCTGGAGGCGGTGCGAGAGATCGGGTGA

Upstream 100 bases:

>100_bases
TTGCCATAATTTCATGGGCAATCTTTGGTTGTATTGTGGACGATAAAAGTGTAGATTATGTCCTGCGTGGTGTGACAATT
TAAGACAAAAGAGGTAGACA

Downstream 100 bases:

>100_bases
GGTGGTGAAGGAATGGAATAGCCTGAATCTCTGATGGATTCAGAAATAGCACGGTAAGGGCTAAGGCCGGGGGATTCAAT
TCTCCCGGCCTTTTTGTGTT

Product: nitrite and sulphite reductase 4Fe-4S region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MMFDPQSVRIDGVYEQRQPGNFMLRIKVPAGIISTEQALRVAEIADRFAGGSVHLTTRGSIELHWLKQENLAEAWRMLAS
VGLTTRGACGGAVRGVVCAMPGDAGYGAAQLLARRLLAHFTHNPRFEGLPKKFKIGVFTGYGDGRHLIQDLSVILREGAD
GNSSYDVWIAGGLGREPHASFLLEEGVAGERLIPLAEAIISLYRELTPKGKRLKHLVRERGREEFVKLVRERVAAIPPLS
LGAGLATPLTSAPREGAAERLTAGIFAGELGTKRFVDLAEIARQYAGGYMVVTGEQNVLFILDDGALRDDAARALAAAGF
GCDSPAERVCFRICPGNHECKMGLSPTRDVARELAGTLGEAGERLSWSICGCPNSCSHPQLADVGIATVKSVKDEQGERH
PLFDLYRRYGTDGFGVVVRHGIGIDELLEAVREIG

Sequences:

>Translated_435_residues
MMFDPQSVRIDGVYEQRQPGNFMLRIKVPAGIISTEQALRVAEIADRFAGGSVHLTTRGSIELHWLKQENLAEAWRMLAS
VGLTTRGACGGAVRGVVCAMPGDAGYGAAQLLARRLLAHFTHNPRFEGLPKKFKIGVFTGYGDGRHLIQDLSVILREGAD
GNSSYDVWIAGGLGREPHASFLLEEGVAGERLIPLAEAIISLYRELTPKGKRLKHLVRERGREEFVKLVRERVAAIPPLS
LGAGLATPLTSAPREGAAERLTAGIFAGELGTKRFVDLAEIARQYAGGYMVVTGEQNVLFILDDGALRDDAARALAAAGF
GCDSPAERVCFRICPGNHECKMGLSPTRDVARELAGTLGEAGERLSWSICGCPNSCSHPQLADVGIATVKSVKDEQGERH
PLFDLYRRYGTDGFGVVVRHGIGIDELLEAVREIG
>Mature_435_residues
MMFDPQSVRIDGVYEQRQPGNFMLRIKVPAGIISTEQALRVAEIADRFAGGSVHLTTRGSIELHWLKQENLAEAWRMLAS
VGLTTRGACGGAVRGVVCAMPGDAGYGAAQLLARRLLAHFTHNPRFEGLPKKFKIGVFTGYGDGRHLIQDLSVILREGAD
GNSSYDVWIAGGLGREPHASFLLEEGVAGERLIPLAEAIISLYRELTPKGKRLKHLVRERGREEFVKLVRERVAAIPPLS
LGAGLATPLTSAPREGAAERLTAGIFAGELGTKRFVDLAEIARQYAGGYMVVTGEQNVLFILDDGALRDDAARALAAAGF
GCDSPAERVCFRICPGNHECKMGLSPTRDVARELAGTLGEAGERLSWSICGCPNSCSHPQLADVGIATVKSVKDEQGERH
PLFDLYRRYGTDGFGVVVRHGIGIDELLEAVREIG

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 46963; Mature: 46963

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFDPQSVRIDGVYEQRQPGNFMLRIKVPAGIISTEQALRVAEIADRFAGGSVHLTTRGS
CCCCCCCEEECCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
IELHWLKQENLAEAWRMLASVGLTTRGACGGAVRGVVCAMPGDAGYGAAQLLARRLLAHF
EEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
THNPRFEGLPKKFKIGVFTGYGDGRHLIQDLSVILREGADGNSSYDVWIAGGLGREPHAS
CCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHH
FLLEEGVAGERLIPLAEAIISLYRELTPKGKRLKHLVRERGREEFVKLVRERVAAIPPLS
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
LGAGLATPLTSAPREGAAERLTAGIFAGELGTKRFVDLAEIARQYAGGYMVVTGEQNVLF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEE
ILDDGALRDDAARALAAAGFGCDSPAERVCFRICPGNHECKMGLSPTRDVARELAGTLGE
EECCCCCCHHHHHHHHHHCCCCCCHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
AGERLSWSICGCPNSCSHPQLADVGIATVKSVKDEQGERHPLFDLYRRYGTDGFGVVVRH
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEHEEC
GIGIDELLEAVREIG
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MMFDPQSVRIDGVYEQRQPGNFMLRIKVPAGIISTEQALRVAEIADRFAGGSVHLTTRGS
CCCCCCCEEECCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
IELHWLKQENLAEAWRMLASVGLTTRGACGGAVRGVVCAMPGDAGYGAAQLLARRLLAHF
EEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
THNPRFEGLPKKFKIGVFTGYGDGRHLIQDLSVILREGADGNSSYDVWIAGGLGREPHAS
CCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHH
FLLEEGVAGERLIPLAEAIISLYRELTPKGKRLKHLVRERGREEFVKLVRERVAAIPPLS
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
LGAGLATPLTSAPREGAAERLTAGIFAGELGTKRFVDLAEIARQYAGGYMVVTGEQNVLF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEE
ILDDGALRDDAARALAAAGFGCDSPAERVCFRICPGNHECKMGLSPTRDVARELAGTLGE
EECCCCCCHHHHHHHHHHCCCCCCHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
AGERLSWSICGCPNSCSHPQLADVGIATVKSVKDEQGERHPLFDLYRRYGTDGFGVVVRH
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEHEEC
GIGIDELLEAVREIG
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA