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The map label for this gene is macA [H]

Identifier: 78223718

GI number: 78223718

Start: 2861199

End: 2862347

Strand: Reverse

Name: macA [H]

Synonym: Gmet_2519

Alternate gene names: 78223718

Gene position: 2862347-2861199 (Counterclockwise)

Preceding gene: 78223719

Following gene: 78223717

Centisome position: 71.6

GC content: 65.01

Gene sequence:

>1149_bases
ATGAACGTATCAAAAATATGCATGCATGCCTTGCTTCTTTTCGCGGTTCCCCTTGCCGGTTGCAAGGGAGACGAAGCGAA
GAAGTCCCCGGCGGTGAAACCGCCGGTGGCGGTGGAGGTCATTGCCGTTGCCCCCCGGGACCTGGTGGAAGGGGTCGAGG
TGACCGGCAGCCTGGAACCGAAGTTCTATGCCGATGTGAAGACCCAGATTCCGGGGCTCGTGAAGCAGGTCTATGTGACT
GAATGGCTGCGGGTCCGCAAGGGGACCCCCCTTGCCCGAATCGACGTGGCCGAGGCGGAGGCAATGGTGAAGCGGGCTGA
GGCGTCGGTGGAGTCGGCCCGGGCCGGCTTGGCCCAGGCCCAGGTGGGGGCAAACCGGGCGGAGCGGGAGCTGACCCGGA
TTCTCAAACTGAAGGAATCGGGGCTCGCGACTCAGCAGGGGGTAGACGACGCCCGCACCGAGTCGGCCGCGGCTCAGGCC
AGGATAGAGGCGGCCCAGGCCCAGATCCGGGTGGCCCAGGAAGAGGTGCGCCAGGCCCGGGCACGGCAGGCAAAGGGGCT
TGTGGTCTCTCCCCTCGATGGAGTAGTGGCCCTGCGGGATGTGAACGTGGGGGATCTGGCAAGCGACGCCGCCGCCAGCA
AGCCCATATTCCGGATAGTGGACAACCGTGTCCTTAACCTGACGGTCACCGTTCCCTCGGCCGATTCGGCCCGGGTCAAG
GTGGGACAGCCGCTGGAGTTCACCGTGGACGCCCTCCCCGGGAAAACCTTCATGGGCAGGGTGATGTACATCAATCCGGA
ACTCTCCTCTGCCGACCGGTCGCTGCGGGTGATCGCCGAAGTGAGAAATGACGGAGACCGCCTCAAGGGAGGGCTCTTCG
CCAAGGGACGGATTATCACTGGCCAGCGGCCCAAGGTGGTTCAGGTCCCCCGTGGGGCCTTGACCACGTGGGATACGGCG
GCCCGGAAGGGGAACCTGTTCATTGTCGCCGGCACCACCGCGAAGCTCAGGCAGGTGGAGACCGGAGCCGTCAGCGGCGA
TTCAGTGGAGATCGTGAAAGGGGTAGGGGCCGGCGAGCAGATCGTGGCCCGCGGAGGCTTCAACCTCAAGGACGGGGATA
CGGTTTCCGTTGCCGGTGGAAGGAAGTAG

Upstream 100 bases:

>100_bases
CCGTAAGAGTGGCGGGACTATACATCCGGTGCGGTATCAGTGTATGCTTGCGTATTGATTAGGCTGCACTAACTGCTGAT
AGTGATGAAGGGGTTAGCTG

Downstream 100 bases:

>100_bases
CGGATGATCCTCTCCGATCTTTCCATCAAGCGTCCCATCTTTGCCGCAGTGATGATGCTGGTGCTTGTGACCCTCGGCAT
CTTCTCCTACCGGCGGCTCT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNVSKICMHALLLFAVPLAGCKGDEAKKSPAVKPPVAVEVIAVAPRDLVEGVEVTGSLEPKFYADVKTQIPGLVKQVYVT
EWLRVRKGTPLARIDVAEAEAMVKRAEASVESARAGLAQAQVGANRAERELTRILKLKESGLATQQGVDDARTESAAAQA
RIEAAQAQIRVAQEEVRQARARQAKGLVVSPLDGVVALRDVNVGDLASDAAASKPIFRIVDNRVLNLTVTVPSADSARVK
VGQPLEFTVDALPGKTFMGRVMYINPELSSADRSLRVIAEVRNDGDRLKGGLFAKGRIITGQRPKVVQVPRGALTTWDTA
ARKGNLFIVAGTTAKLRQVETGAVSGDSVEIVKGVGAGEQIVARGGFNLKDGDTVSVAGGRK

Sequences:

>Translated_382_residues
MNVSKICMHALLLFAVPLAGCKGDEAKKSPAVKPPVAVEVIAVAPRDLVEGVEVTGSLEPKFYADVKTQIPGLVKQVYVT
EWLRVRKGTPLARIDVAEAEAMVKRAEASVESARAGLAQAQVGANRAERELTRILKLKESGLATQQGVDDARTESAAAQA
RIEAAQAQIRVAQEEVRQARARQAKGLVVSPLDGVVALRDVNVGDLASDAAASKPIFRIVDNRVLNLTVTVPSADSARVK
VGQPLEFTVDALPGKTFMGRVMYINPELSSADRSLRVIAEVRNDGDRLKGGLFAKGRIITGQRPKVVQVPRGALTTWDTA
ARKGNLFIVAGTTAKLRQVETGAVSGDSVEIVKGVGAGEQIVARGGFNLKDGDTVSVAGGRK
>Mature_382_residues
MNVSKICMHALLLFAVPLAGCKGDEAKKSPAVKPPVAVEVIAVAPRDLVEGVEVTGSLEPKFYADVKTQIPGLVKQVYVT
EWLRVRKGTPLARIDVAEAEAMVKRAEASVESARAGLAQAQVGANRAERELTRILKLKESGLATQQGVDDARTESAAAQA
RIEAAQAQIRVAQEEVRQARARQAKGLVVSPLDGVVALRDVNVGDLASDAAASKPIFRIVDNRVLNLTVTVPSADSARVK
VGQPLEFTVDALPGKTFMGRVMYINPELSSADRSLRVIAEVRNDGDRLKGGLFAKGRIITGQRPKVVQVPRGALTTWDTA
ARKGNLFIVAGTTAKLRQVETGAVSGDSVEIVKGVGAGEQIVARGGFNLKDGDTVSVAGGRK

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=331, Percent_Identity=28.3987915407855, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789929, Length=376, Percent_Identity=23.4042553191489, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082036, Length=322, Percent_Identity=24.5341614906832, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40434; Mature: 40434

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSKICMHALLLFAVPLAGCKGDEAKKSPAVKPPVAVEVIAVAPRDLVEGVEVTGSLEP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHCCCEECCCCCC
KFYADVKTQIPGLVKQVYVTEWLRVRKGTPLARIDVAEAEAMVKRAEASVESARAGLAQA
CEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
QVGANRAERELTRILKLKESGLATQQGVDDARTESAAAQARIEAAQAQIRVAQEEVRQAR
HHCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQAKGLVVSPLDGVVALRDVNVGDLASDAAASKPIFRIVDNRVLNLTVTVPSADSARVK
HHHHCCEEEECCCCEEEEEECCCCCCCHHHHCCCCHHEECCCEEEEEEEEECCCCCCEEE
VGQPLEFTVDALPGKTFMGRVMYINPELSSADRSLRVIAEVRNDGDRLKGGLFAKGRIIT
ECCCCEEEEECCCCCHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCHHCCCEEECCEEEE
GQRPKVVQVPRGALTTWDTAARKGNLFIVAGTTAKLRQVETGAVSGDSVEIVKGVGAGEQ
CCCCCEEECCCCCCCCCHHHCCCCCEEEEECCCHHHHEECCCCCCCCCEEEEECCCCCCC
IVARGGFNLKDGDTVSVAGGRK
EEECCCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MNVSKICMHALLLFAVPLAGCKGDEAKKSPAVKPPVAVEVIAVAPRDLVEGVEVTGSLEP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHCCCEECCCCCC
KFYADVKTQIPGLVKQVYVTEWLRVRKGTPLARIDVAEAEAMVKRAEASVESARAGLAQA
CEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
QVGANRAERELTRILKLKESGLATQQGVDDARTESAAAQARIEAAQAQIRVAQEEVRQAR
HHCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQAKGLVVSPLDGVVALRDVNVGDLASDAAASKPIFRIVDNRVLNLTVTVPSADSARVK
HHHHCCEEEECCCCEEEEEECCCCCCCHHHHCCCCHHEECCCEEEEEEEEECCCCCCEEE
VGQPLEFTVDALPGKTFMGRVMYINPELSSADRSLRVIAEVRNDGDRLKGGLFAKGRIIT
ECCCCEEEEECCCCCHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCHHCCCEEECCEEEE
GQRPKVVQVPRGALTTWDTAARKGNLFIVAGTTAKLRQVETGAVSGDSVEIVKGVGAGEQ
CCCCCEEECCCCCCCCCHHHCCCCCEEEEECCCHHHHEECCCCCCCCCEEEEECCCCCCC
IVARGGFNLKDGDTVSVAGGRK
EEECCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]