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The map label for this gene is trpB2 [H]

Identifier: 78223694

GI number: 78223694

Start: 2835098

End: 2836453

Strand: Reverse

Name: trpB2 [H]

Synonym: Gmet_2493

Alternate gene names: 78223694

Gene position: 2836453-2835098 (Counterclockwise)

Preceding gene: 78223695

Following gene: 78223693

Centisome position: 70.96

GC content: 66.52

Gene sequence:

>1356_bases
ATGCAGACAAAAATCCTGCTCGACGAAAGCCGCATCCCCACCCACTGGTATAACATCATCCCCGACATGCCCGGCCCCCT
GGCGCCAGTCATCAATCCCCGGACCCTCCAGCCGGTGACACCCGACGACCTCCTCCCCATCTTCCCCATGGCCATTATCG
AGCAGGAGGTCTCGGGCGAGCGGTGGATCCCGATTCCCGAGGAGGTGCGGGAGATCTACCGGCTCTGGCGGCCGTCACCC
CTCTACCGGGCGCGGCGGCTGGAGCAGGCCCTCGGCACCCCGGCGAAGATCTACTACAAGTACGAAGGGGTGTCCCCGGC
CGGCTCCCACAAGCCCAATTCGGCCATCCCCCAGGCCTTCTACAACAAGCAGGCCGGCATCCGTCGCCTCGCCACCGAGA
CCGGCGCCGGCCAGTGGGGGAGTTCCCTGGCCCTGGCCTGCTCCATGTTCGGCCTTGAGTGCACGGTCTACATGGTGAAA
GTGTCGTGCACCCAGAAGCCCTACCGCAAGAGCATGATGCAGCTCTGGGGGGCCAATGTGATCCCGTCGCCCTCTGAGTT
CACCAACGCCGGCCGTGCCATCCTGGCCCACGACCCGGACAGCAACGGGAGCCTCGGCATCGCCATCTCCGAGGCTGTGG
AGGACGCTGTCTCCCGCCCCGACACCAACTACGCCCTGGGGAGCGTCCTGAACCACGTCTGCCTCCACCAGACCGTCATC
GGCCAGGAGGCCAAGGAGCAGCTGGCCCTGGCCGGCGACTACCCAGACGTGGTCATCGCCTGCTGCGGCGGCGGCTCCAA
CTTCGCCGGCACGGCGTTCCCGTTCCTGGCCGACCGAGCCGCCGGCAAGAACGTCCGCTGCCTGGCCGTGGAGCCCGCCT
CCTGCCCGACCCTCACCAAGGGGGTCTATGCCTTCGACTACGGCGACACCGCCAAGATGGCCCCCATCGCCATGATGTAC
ACCCTGGGCCACGACTTCATGCCGCCGGGGATCCACGCTGGGGGCTTGCGCTACCATGGCGAGTCCGCCCTGGTGTCGCA
GCTCCATCACGCGGGGCTCATCGAGGCGAAGTCGTACCGTCAGAACGCCTGTTTTGAGGCGGCCCACCTCTTTGCCCGCA
GCGAGGGGATCGTCCCGGCCCCCGAATCGTCCCACGCGGTGCGGGCTGCCATTGACGAAGCGGTCCTCGCCAAGGAAGAG
GGAAAGGAAAAGACTATCCTTTTCTGCCTCTCGGGCCACGGCCAGCTGGACATGGGGGCCTACGACGCCTACCTCTCCGG
CGGGCTGGAGGATTTCGAGTATCCCGAGGAGATGATTCGGGAGTCCCTGGCGCGGCTGCCGAAGGTGGATCTGTAA

Upstream 100 bases:

>100_bases
GATGCTTCTGCTTCTATTGATAGGATCAGGCGTTATAAATTCATTGTTTTTCTCTGTTGCTCAGTTGCTCTGTGTTATTC
CTTAACAAGCCGAGGTTTCC

Downstream 100 bases:

>100_bases
GCGCATGGTTCGCGTCAAGATCTGCGGCATAACCTCCCTGGAGGACGCCCTCATGGCGGTGGAGGCCGGAGCCGATGCCC
TCGGCTTCGTCTTCCATGAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQTKILLDESRIPTHWYNIIPDMPGPLAPVINPRTLQPVTPDDLLPIFPMAIIEQEVSGERWIPIPEEVREIYRLWRPSP
LYRARRLEQALGTPAKIYYKYEGVSPAGSHKPNSAIPQAFYNKQAGIRRLATETGAGQWGSSLALACSMFGLECTVYMVK
VSCTQKPYRKSMMQLWGANVIPSPSEFTNAGRAILAHDPDSNGSLGIAISEAVEDAVSRPDTNYALGSVLNHVCLHQTVI
GQEAKEQLALAGDYPDVVIACCGGGSNFAGTAFPFLADRAAGKNVRCLAVEPASCPTLTKGVYAFDYGDTAKMAPIAMMY
TLGHDFMPPGIHAGGLRYHGESALVSQLHHAGLIEAKSYRQNACFEAAHLFARSEGIVPAPESSHAVRAAIDEAVLAKEE
GKEKTILFCLSGHGQLDMGAYDAYLSGGLEDFEYPEEMIRESLARLPKVDL

Sequences:

>Translated_451_residues
MQTKILLDESRIPTHWYNIIPDMPGPLAPVINPRTLQPVTPDDLLPIFPMAIIEQEVSGERWIPIPEEVREIYRLWRPSP
LYRARRLEQALGTPAKIYYKYEGVSPAGSHKPNSAIPQAFYNKQAGIRRLATETGAGQWGSSLALACSMFGLECTVYMVK
VSCTQKPYRKSMMQLWGANVIPSPSEFTNAGRAILAHDPDSNGSLGIAISEAVEDAVSRPDTNYALGSVLNHVCLHQTVI
GQEAKEQLALAGDYPDVVIACCGGGSNFAGTAFPFLADRAAGKNVRCLAVEPASCPTLTKGVYAFDYGDTAKMAPIAMMY
TLGHDFMPPGIHAGGLRYHGESALVSQLHHAGLIEAKSYRQNACFEAAHLFARSEGIVPAPESSHAVRAAIDEAVLAKEE
GKEKTILFCLSGHGQLDMGAYDAYLSGGLEDFEYPEEMIRESLARLPKVDL
>Mature_451_residues
MQTKILLDESRIPTHWYNIIPDMPGPLAPVINPRTLQPVTPDDLLPIFPMAIIEQEVSGERWIPIPEEVREIYRLWRPSP
LYRARRLEQALGTPAKIYYKYEGVSPAGSHKPNSAIPQAFYNKQAGIRRLATETGAGQWGSSLALACSMFGLECTVYMVK
VSCTQKPYRKSMMQLWGANVIPSPSEFTNAGRAILAHDPDSNGSLGIAISEAVEDAVSRPDTNYALGSVLNHVCLHQTVI
GQEAKEQLALAGDYPDVVIACCGGGSNFAGTAFPFLADRAAGKNVRCLAVEPASCPTLTKGVYAFDYGDTAKMAPIAMMY
TLGHDFMPPGIHAGGLRYHGESALVSQLHHAGLIEAKSYRQNACFEAAHLFARSEGIVPAPESSHAVRAAIDEAVLAKEE
GKEKTILFCLSGHGQLDMGAYDAYLSGGLEDFEYPEEMIRESLARLPKVDL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=359, Percent_Identity=29.8050139275766, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321412, Length=378, Percent_Identity=31.2169312169312, Blast_Score=131, Evalue=3e-31,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 49065; Mature: 49065

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKILLDESRIPTHWYNIIPDMPGPLAPVINPRTLQPVTPDDLLPIFPMAIIEQEVSGE
CCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
RWIPIPEEVREIYRLWRPSPLYRARRLEQALGTPAKIYYKYEGVSPAGSHKPNSAIPQAF
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHH
YNKQAGIRRLATETGAGQWGSSLALACSMFGLECTVYMVKVSCTQKPYRKSMMQLWGANV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHCCCC
IPSPSEFTNAGRAILAHDPDSNGSLGIAISEAVEDAVSRPDTNYALGSVLNHVCLHQTVI
CCCCHHHHCCCCEEEEECCCCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GQEAKEQLALAGDYPDVVIACCGGGSNFAGTAFPFLADRAAGKNVRCLAVEPASCPTLTK
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHC
GVYAFDYGDTAKMAPIAMMYTLGHDFMPPGIHAGGLRYHGESALVSQLHHAGLIEAKSYR
CEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCHHHHHHH
QNACFEAAHLFARSEGIVPAPESSHAVRAAIDEAVLAKEEGKEKTILFCLSGHGQLDMGA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCH
YDAYLSGGLEDFEYPEEMIRESLARLPKVDL
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQTKILLDESRIPTHWYNIIPDMPGPLAPVINPRTLQPVTPDDLLPIFPMAIIEQEVSGE
CCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
RWIPIPEEVREIYRLWRPSPLYRARRLEQALGTPAKIYYKYEGVSPAGSHKPNSAIPQAF
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHH
YNKQAGIRRLATETGAGQWGSSLALACSMFGLECTVYMVKVSCTQKPYRKSMMQLWGANV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHCCCC
IPSPSEFTNAGRAILAHDPDSNGSLGIAISEAVEDAVSRPDTNYALGSVLNHVCLHQTVI
CCCCHHHHCCCCEEEEECCCCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GQEAKEQLALAGDYPDVVIACCGGGSNFAGTAFPFLADRAAGKNVRCLAVEPASCPTLTK
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHC
GVYAFDYGDTAKMAPIAMMYTLGHDFMPPGIHAGGLRYHGESALVSQLHHAGLIEAKSYR
CEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCHHHHHHH
QNACFEAAHLFARSEGIVPAPESSHAVRAAIDEAVLAKEEGKEKTILFCLSGHGQLDMGA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCH
YDAYLSGGLEDFEYPEEMIRESLARLPKVDL
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]