Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 78223678

GI number: 78223678

Start: 2815423

End: 2816697

Strand: Reverse

Name: folC [H]

Synonym: Gmet_2475

Alternate gene names: 78223678

Gene position: 2816697-2815423 (Counterclockwise)

Preceding gene: 78223679

Following gene: 78223677

Centisome position: 70.46

GC content: 65.18

Gene sequence:

>1275_bases
ATGACCTACGAGGAGACCCTGACACATATCTACGGCCTGAGGCGTTTCGGGATCAAACCCGGGCTCGAACGGATCGCCAC
GCTTCTGGAGAGGCTCGGCAATCCCCACGAGCGGCTGCGGGTCGTTCACGTGGCAGGGACCAACGGCAAGGGGTCCACGG
CGGCGTTCCTGGCCTCCATCCTGACGACAGGCGGGTATCGGACCGGCCTCTTCACTTCTCCCCATCTCACCCGATTCACC
GAACGGATTCGCATCGACGGCACCGAGATCGCCGAGGATGCGGTTTGCCGCATTGCCGGGCTGGTCATGGCTGCCGCCCC
GGATGGCACCACCTTTTTCGAGATCGTGACCGCCATGGCGCTCCGTTATTTTGCCGAGGAGCAGGTGACTGTCGCCATTC
TCGAAGCGGGTATGGGGGGAGGCTCCGACGCCACCAGCGCCGCTTTCGGAATCCTTTCCGTCATCACTCCCGTGGCCCTT
GATCACTGCGTGTGGCTCGGCGATTCGCTGGCGGCAATCGCCCGGGAGAAGGCCGGCATCATCAGGCCGGGGAGGCCGGT
GGTACTTGCGGAGCAGCAGGCGGAGGTTCTTGACGTCATCAAGGAGCGGAGCACGGCCCTCAATAGCCCGTTGGTACGCT
TCGGCAGCGATTTCGGCGCATCTTGGGGAACGGATGGGCTTGACTACCAGGGGATGGGACGGGTGCTGTCAGGTCTCACA
CTGGGAATCGGCGGGCGTTATCAGGCCGTGAATGCGGCGACTGCCCTGGCTGCCGCGGAGCTTCTGACCACCGTCGGCTA
CTCACTTGCGGATGAAGCGCTGCGCAGCGGTATCTCCCAAGCCCGCTGGCCAGGGAGAATGGAATATTTTGCCGGCCCCC
CCCGGATCATTTTTGATGGGGCCCACAATCCGGCAGGTGCCTTGGCCCTGGCCGAATCCCTGGCGGGAGTGCCGCGCCGG
CGTCTGATCGTGGTGGTCGGGGTCATGGGGGATAAGGATGCCGCCGGAATCCTCGGGCCGCTCCTTCCCCTTACTGACGA
GGTGGTGGCGGTTACCCCGGCGGTGGAGCGGGGGTTGTCCTCGGCCGAGCTTGCCGCCCTGTGCCGCCGGTTGGGCGTTT
CCGCTACCGACGGCGGACGCGTGCCCGAGGGGCTTGCCATGGCCCGCAACATGGCCGCGGTCGATGATCTGATCCTTGTC
TGCGGCTCCCTCTTTACGGTGGGAGAGGCCCGGTCGGCGCTTCTCGCCCAGCGGTTCGAACCTTTCCGAGGATAA

Upstream 100 bases:

>100_bases
GATTGACCGGCAGGGGCGGAGGTGTTCTCTCCGCCCCTGTTGCTTTCGTGGGTCAAAGCGGTGGATTTTCCCCCTCTCTT
CCCGTATAATCCCGCCCGTC

Downstream 100 bases:

>100_bases
GTGACAGTTACCCATGGGAATTTTTTCATACAGACACCTCCTTCTCTTTGCGCCAGTTTTTCTTTCGGTACCGGCAGCGG
CGTTAGCCCAGGGCGACGCG

Product: folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTYEETLTHIYGLRRFGIKPGLERIATLLERLGNPHERLRVVHVAGTNGKGSTAAFLASILTTGGYRTGLFTSPHLTRFT
ERIRIDGTEIAEDAVCRIAGLVMAAAPDGTTFFEIVTAMALRYFAEEQVTVAILEAGMGGGSDATSAAFGILSVITPVAL
DHCVWLGDSLAAIAREKAGIIRPGRPVVLAEQQAEVLDVIKERSTALNSPLVRFGSDFGASWGTDGLDYQGMGRVLSGLT
LGIGGRYQAVNAATALAAAELLTTVGYSLADEALRSGISQARWPGRMEYFAGPPRIIFDGAHNPAGALALAESLAGVPRR
RLIVVVGVMGDKDAAGILGPLLPLTDEVVAVTPAVERGLSSAELAALCRRLGVSATDGGRVPEGLAMARNMAAVDDLILV
CGSLFTVGEARSALLAQRFEPFRG

Sequences:

>Translated_424_residues
MTYEETLTHIYGLRRFGIKPGLERIATLLERLGNPHERLRVVHVAGTNGKGSTAAFLASILTTGGYRTGLFTSPHLTRFT
ERIRIDGTEIAEDAVCRIAGLVMAAAPDGTTFFEIVTAMALRYFAEEQVTVAILEAGMGGGSDATSAAFGILSVITPVAL
DHCVWLGDSLAAIAREKAGIIRPGRPVVLAEQQAEVLDVIKERSTALNSPLVRFGSDFGASWGTDGLDYQGMGRVLSGLT
LGIGGRYQAVNAATALAAAELLTTVGYSLADEALRSGISQARWPGRMEYFAGPPRIIFDGAHNPAGALALAESLAGVPRR
RLIVVVGVMGDKDAAGILGPLLPLTDEVVAVTPAVERGLSSAELAALCRRLGVSATDGGRVPEGLAMARNMAAVDDLILV
CGSLFTVGEARSALLAQRFEPFRG
>Mature_423_residues
TYEETLTHIYGLRRFGIKPGLERIATLLERLGNPHERLRVVHVAGTNGKGSTAAFLASILTTGGYRTGLFTSPHLTRFTE
RIRIDGTEIAEDAVCRIAGLVMAAAPDGTTFFEIVTAMALRYFAEEQVTVAILEAGMGGGSDATSAAFGILSVITPVALD
HCVWLGDSLAAIAREKAGIIRPGRPVVLAEQQAEVLDVIKERSTALNSPLVRFGSDFGASWGTDGLDYQGMGRVLSGLTL
GIGGRYQAVNAATALAAAELLTTVGYSLADEALRSGISQARWPGRMEYFAGPPRIIFDGAHNPAGALALAESLAGVPRRR
LIVVVGVMGDKDAAGILGPLLPLTDEVVAVTPAVERGLSSAELAALCRRLGVSATDGGRVPEGLAMARNMAAVDDLILVC
GSLFTVGEARSALLAQRFEPFRG

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=362, Percent_Identity=29.0055248618785, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI66932984, Length=362, Percent_Identity=29.0055248618785, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1788654, Length=420, Percent_Identity=34.5238095238095, Blast_Score=204, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI17553148, Length=364, Percent_Identity=27.4725274725275, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71984923, Length=364, Percent_Identity=27.4725274725275, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17553150, Length=364, Percent_Identity=28.021978021978, Blast_Score=125, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323760, Length=438, Percent_Identity=31.0502283105023, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6324815, Length=406, Percent_Identity=26.3546798029557, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322718, Length=294, Percent_Identity=26.1904761904762, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24641571, Length=294, Percent_Identity=31.9727891156463, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24581568, Length=201, Percent_Identity=33.8308457711443, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44513; Mature: 44382

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYEETLTHIYGLRRFGIKPGLERIATLLERLGNPHERLRVVHVAGTNGKGSTAAFLASI
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHEEEEEEECCCCCCHHHHHHHHH
LTTGGYRTGLFTSPHLTRFTERIRIDGTEIAEDAVCRIAGLVMAAAPDGTTFFEIVTAMA
HHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
LRYFAEEQVTVAILEAGMGGGSDATSAAFGILSVITPVALDHCVWLGDSLAAIAREKAGI
HHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCC
IRPGRPVVLAEQQAEVLDVIKERSTALNSPLVRFGSDFGASWGTDGLDYQGMGRVLSGLT
CCCCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCE
LGIGGRYQAVNAATALAAAELLTTVGYSLADEALRSGISQARWPGRMEYFAGPPRIIFDG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEEEC
AHNPAGALALAESLAGVPRRRLIVVVGVMGDKDAAGILGPLLPLTDEVVAVTPAVERGLS
CCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCEEECHHHHHCCC
SAELAALCRRLGVSATDGGRVPEGLAMARNMAAVDDLILVCGSLFTVGEARSALLAQRFE
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PFRG
CCCC
>Mature Secondary Structure 
TYEETLTHIYGLRRFGIKPGLERIATLLERLGNPHERLRVVHVAGTNGKGSTAAFLASI
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHEEEEEEECCCCCCHHHHHHHHH
LTTGGYRTGLFTSPHLTRFTERIRIDGTEIAEDAVCRIAGLVMAAAPDGTTFFEIVTAMA
HHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
LRYFAEEQVTVAILEAGMGGGSDATSAAFGILSVITPVALDHCVWLGDSLAAIAREKAGI
HHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCC
IRPGRPVVLAEQQAEVLDVIKERSTALNSPLVRFGSDFGASWGTDGLDYQGMGRVLSGLT
CCCCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCE
LGIGGRYQAVNAATALAAAELLTTVGYSLADEALRSGISQARWPGRMEYFAGPPRIIFDG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEEEC
AHNPAGALALAESLAGVPRRRLIVVVGVMGDKDAAGILGPLLPLTDEVVAVTPAVERGLS
CCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCEEECHHHHHCCC
SAELAALCRRLGVSATDGGRVPEGLAMARNMAAVDDLILVCGSLFTVGEARSALLAQRFE
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PFRG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]