Click here to switch to the map view.

The map label for this gene is prfB [H]

Identifier: 78223569

GI number: 78223569

Start: 2673697

End: 2674791

Strand: Reverse

Name: prfB [H]

Synonym: Gmet_2366

Alternate gene names: 78223569

Gene position: 2674791-2673697 (Counterclockwise)

Preceding gene: 78223570

Following gene: 78223568

Centisome position: 66.91

GC content: 61.28

Gene sequence:

>1095_bases
TTGAAAATCTTTCGGAGCGCATCGCCAAGCTCCGGGGGTCTCTTTGACATAGACAGCAAGCGGGAGGATATCCAGGAGAT
GGAGGCCCATATCGCGGCCCCCGGCTTCTGGGATGACAACGAGAAGGCCCAGCAACTCCTCAGGGAACGGACGCGGATCG
AGAAGATCCTGGAGCTCTGGGACCGTCTGACCCGTCAGGCCGACGATATCCGGGTGCTGATCGAGCTGGGCGAGGAGGCC
GGGGACGAGGGAACCCTCGACGAGGTCCGGGCACTCAATGACCAGCTGGAAAAGGATGTGGAGGGGGCCGAGTTCCAGCG
GATGCTCTCGGGCACCCACGACAAGAACGCCTGCTACTTTTCCATCAACGCCGGCGCAGGGGGTACCGAGGCCCAGGACT
GGGCCGAGATGCTGCTCAGGATGTATCTCCGCTACTGCGAGCGCAAGGGGTGGAAAACGGAGATCACCGACTATCAGGGC
GGCGACGAGGCTGGGGTCAAGTCGGCCACCTTTACCGTTGACGGTGAGTATGCATACGGCTATCTTAAGGCCGAAGCCGG
CATCCACCGCCTCGTGCGGATTTCTCCTTTCGATTCCAATGCCCGGCGACATACCTCCTTCGCCTCGGTCTTCGCCTTCC
CCGAGATCGAGGACGATATCGAGGTAACGATCAACGAAGCAGATCTGAGAATCGACACCTACCGCTCCAGCGGGGCCGGC
GGCCAGCACGTGAATACTACCGATTCAGCCGTCCGGATCACCCATATCCCCACGGGCCTCGTGGCTGCCAGCCAGTGCGA
GCGGAGCCAGCACATGAACAAGGCGACCGCCATGAAGATGCTCCGGGCCAAGCTCTACGAGAAGGAGCTTCAGGAGCGGG
AGGCCAAGGCCGCCGAGATCGGCGGTGAGAAGAAGGAGATCGGCTGGGGAAGCCAGATTCGCTCCTATGTCCTTCATCCC
TACAAGATGGTGAAGGATCTGAGGACCGGCGTTGAAACCGGCAACCCCGACGCGGTTCTGGACGGCGATCTCGAGGAGTT
CATCGTCGCCTTCCTCATGGGGGTACGCCGGGACGTTAAGGACACCGTAGACTGA

Upstream 100 bases:

>100_bases
TGTTGCGGGCGCGGCGGTGGTTGCGGCCCTTGCTTTCAGAAGAAAGAGTATTCACCATCAATAAAGGAGTCATGGATGTT
CAGAGAAGAAGTAGCCCGCG

Downstream 100 bases:

>100_bases
TGAAGAGATTCGTTGTCCATCTGATAGCATTTATTGTCGTCTCCCTCCTGGCGGCCGCCGGCTGTGCCGCGCCGCGGGCG
GGAATCTCCCGCATCGGCCA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKIFRSASPSSGGLFDIDSKREDIQEMEAHIAAPGFWDDNEKAQQLLRERTRIEKILELWDRLTRQADDIRVLIELGEEA
GDEGTLDEVRALNDQLEKDVEGAEFQRMLSGTHDKNACYFSINAGAGGTEAQDWAEMLLRMYLRYCERKGWKTEITDYQG
GDEAGVKSATFTVDGEYAYGYLKAEAGIHRLVRISPFDSNARRHTSFASVFAFPEIEDDIEVTINEADLRIDTYRSSGAG
GQHVNTTDSAVRITHIPTGLVAASQCERSQHMNKATAMKMLRAKLYEKELQEREAKAAEIGGEKKEIGWGSQIRSYVLHP
YKMVKDLRTGVETGNPDAVLDGDLEEFIVAFLMGVRRDVKDTVD

Sequences:

>Translated_364_residues
MKIFRSASPSSGGLFDIDSKREDIQEMEAHIAAPGFWDDNEKAQQLLRERTRIEKILELWDRLTRQADDIRVLIELGEEA
GDEGTLDEVRALNDQLEKDVEGAEFQRMLSGTHDKNACYFSINAGAGGTEAQDWAEMLLRMYLRYCERKGWKTEITDYQG
GDEAGVKSATFTVDGEYAYGYLKAEAGIHRLVRISPFDSNARRHTSFASVFAFPEIEDDIEVTINEADLRIDTYRSSGAG
GQHVNTTDSAVRITHIPTGLVAASQCERSQHMNKATAMKMLRAKLYEKELQEREAKAAEIGGEKKEIGWGSQIRSYVLHP
YKMVKDLRTGVETGNPDAVLDGDLEEFIVAFLMGVRRDVKDTVD
>Mature_364_residues
MKIFRSASPSSGGLFDIDSKREDIQEMEAHIAAPGFWDDNEKAQQLLRERTRIEKILELWDRLTRQADDIRVLIELGEEA
GDEGTLDEVRALNDQLEKDVEGAEFQRMLSGTHDKNACYFSINAGAGGTEAQDWAEMLLRMYLRYCERKGWKTEITDYQG
GDEAGVKSATFTVDGEYAYGYLKAEAGIHRLVRISPFDSNARRHTSFASVFAFPEIEDDIEVTINEADLRIDTYRSSGAG
GQHVNTTDSAVRITHIPTGLVAASQCERSQHMNKATAMKMLRAKLYEKELQEREAKAAEIGGEKKEIGWGSQIRSYVLHP
YKMVKDLRTGVETGNPDAVLDGDLEEFIVAFLMGVRRDVKDTVD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=245, Percent_Identity=39.1836734693878, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI34577120, Length=223, Percent_Identity=38.5650224215247, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI166795305, Length=145, Percent_Identity=42.7586206896552, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=54.4117647058823, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787462, Length=238, Percent_Identity=42.8571428571429, Blast_Score=181, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=209, Percent_Identity=34.9282296650718, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=240, Percent_Identity=37.0833333333333, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19921226, Length=217, Percent_Identity=44.7004608294931, Blast_Score=174, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40916; Mature: 40916

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFRSASPSSGGLFDIDSKREDIQEMEAHIAAPGFWDDNEKAQQLLRERTRIEKILELW
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DRLTRQADDIRVLIELGEEAGDEGTLDEVRALNDQLEKDVEGAEFQRMLSGTHDKNACYF
HHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEE
SINAGAGGTEAQDWAEMLLRMYLRYCERKGWKTEITDYQGGDEAGVKSATFTVDGEYAYG
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEE
YLKAEAGIHRLVRISPFDSNARRHTSFASVFAFPEIEDDIEVTINEADLRIDTYRSSGAG
EEEHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCC
GQHVNTTDSAVRITHIPTGLVAASQCERSQHMNKATAMKMLRAKLYEKELQEREAKAAEI
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGEKKEIGWGSQIRSYVLHPYKMVKDLRTGVETGNPDAVLDGDLEEFIVAFLMGVRRDVK
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHH
DTVD
HCCC
>Mature Secondary Structure
MKIFRSASPSSGGLFDIDSKREDIQEMEAHIAAPGFWDDNEKAQQLLRERTRIEKILELW
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DRLTRQADDIRVLIELGEEAGDEGTLDEVRALNDQLEKDVEGAEFQRMLSGTHDKNACYF
HHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEE
SINAGAGGTEAQDWAEMLLRMYLRYCERKGWKTEITDYQGGDEAGVKSATFTVDGEYAYG
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEE
YLKAEAGIHRLVRISPFDSNARRHTSFASVFAFPEIEDDIEVTINEADLRIDTYRSSGAG
EEEHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCC
GQHVNTTDSAVRITHIPTGLVAASQCERSQHMNKATAMKMLRAKLYEKELQEREAKAAEI
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGEKKEIGWGSQIRSYVLHPYKMVKDLRTGVETGNPDAVLDGDLEEFIVAFLMGVRRDVK
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHH
DTVD
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA